buds
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- MeSH
- končetiny růst a vývoj MeSH
- kultivační média MeSH
- kyselina askorbová MeSH
- myši MeSH
- Check Tag
- myši MeSH
Acta oto-laryngologica, ISSN 0365-5237 suppl. 259, 1969
95 s. : il., tab. ; 26 cm
Rat hypodactyly (hd) is an autosomal recessive mutation manifesting in homozygotes as reduction or loss of digits II and III. We mapped the hd allele to a short segment of chromosome 10, containing 16 genes. None of these genes has been shown to influence limb development yet. In situ hybridization showed no changes in several important patterning genes (Shh, Fgf8, Bmp2, 4, 7). However, we found that expression of cartilage condensation marker Sox9, and Bmp receptor Bmpr1b (acting as an upstream activator of Sox9 expression) is absent from the subepithelial mesenchyme of the digit condensations II and III. The failure of the chondrogenic condensations to extend towards the subepithelial mesenchyme may reduce the size of digit primordia and underlie the subsequent loss of phalanges and reduction of metacarpals/metatarsals in hd rats.
- MeSH
- embryo savčí anatomie a histologie metabolismus MeSH
- fenotyp MeSH
- financování organizované MeSH
- homeodoménové proteiny genetika metabolismus MeSH
- končetinové pupeny abnormality metabolismus MeSH
- končetiny MeSH
- krysa rodu rattus MeSH
- mutace MeSH
- potkani Wistar MeSH
- receptory morfogenetických kostních proteinů typu I genetika metabolismus MeSH
- rozvržení tělního plánu genetika MeSH
- transkripční faktor SOX9 genetika metabolismus MeSH
- vývojová regulace genové exprese MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
Apical dominance is one of the fundamental developmental phenomena in plant biology, which determines the overall architecture of aerial plant parts. Here we show apex decapitation activated competition for dominance in adjacent upper and lower axillary buds. A two-nodal-bud pea (Pisum sativum L.) was used as a model system to monitor and assess auxin flow, auxin transport channels, and dormancy and initiation status of axillary buds. Auxin flow was manipulated by lateral stem wounds or chemically by auxin efflux inhibitors 2,3,5-triiodobenzoic acid (TIBA), 1-N-naphtylphtalamic acid (NPA), or protein synthesis inhibitor cycloheximide (CHX) treatments, which served to interfere with axillary bud competition. Redirecting auxin flow to different points influenced which bud formed the outgrowing and dominant shoot. The obtained results proved that competition between upper and lower axillary buds as secondary auxin sources is based on the same auxin canalization principle that operates between the shoot apex and axillary bud.
- MeSH
- biologický transport MeSH
- hrách setý účinky léků genetika růst a vývoj MeSH
- kyseliny indoloctové farmakologie MeSH
- regulace genové exprese u rostlin účinky léků MeSH
- regulátory růstu rostlin farmakologie MeSH
- rostlinné proteiny genetika MeSH
- stonky rostlin účinky léků genetika růst a vývoj MeSH
- výhonky rostlin účinky léků genetika růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Shoot branching is one of the major determinants of plant architecture. Polar auxin transport in stems is necessary for the control of bud outgrowth by a dominant apex. Here, we show that following decapitation in pea (Pisum sativum L.), the axillary buds establish directional auxin export by subcellular polarization of PIN auxin transporters. Apical auxin application on the decapitated stem prevents this PIN polarization and canalization of laterally applied auxin. These results support a model in which the apical and lateral auxin sources compete for primary channels of auxin transport in the stem to control the outgrowth of axillary buds.
- MeSH
- hrách setý genetika růst a vývoj metabolismus MeSH
- kyseliny indoloctové metabolismus MeSH
- regulace genové exprese u rostlin MeSH
- regulátory růstu rostlin metabolismus MeSH
- rostlinné proteiny genetika metabolismus MeSH
- stonky rostlin růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
