electron probe microanalysis Dotaz Zobrazit nápovědu

Analýza databázy PubMed ukázala nedostatok informácií o rôntgenovej mikroanalýze (RtgMA) ľudskej sleziny. Vyšetrili sme slezinu pacienta postihnutého autoimúnnou trombocytopenickou purpurou. Na REM štúdium slezmy a na RtgMA sa pripravili vzorky fixované glutaraldehydom a pokovené meďou. Na RtgMA sme použili detektor QUANTUM a analyzátor DELTA-CLASS. V slezine fixovanej GA a pokrytej meďou sme okrem kremíka našli rôzne spektrálne čiary prvkov, ktoré sú obvykle prítomné v živočíšnych tkanivách. Biologický význam takého poznatku zostal pre nás zatiaľ nejasný.

There is a lack of information on x-ray microanalysis (XMA) of the human spleen. We have examined the spleen of a patient with autoimmune thrombocytopenic purpura. The specimens were fixed in glutaraldehyde and coated by copper. The XMA was performed by a Quantum detector Delta-Class analyzer. In addition to spectral lines of elements normally present m human tissues, we have hemonstrated silicon. The presence of silicon in human spleen may be a consequence of the organ's cleansing function.

MeSH
idiopatická trombocytopenická purpura MeSH
křemík analýza MeSH
lidé MeSH
mikroanalýza elektronovou sondou MeSH
mikroskopie elektronová rastrovací MeSH
slezina patologie MeSH
splenektomie MeSH
Check Tag
lidé MeSH

Článek

In-situ preparation of plant samples in ESEM for energy dispersive x-ray microanalysis and repetitive observation in SEM and ESEM

Temperature Method (ELTM) for the in-situ preparation of plant samples in an environmental scanning electron ...

Scientific reports. 2019 ; 9 (1) : 2300. [pub] 20190219

Sci Rep
ISSN 2045-2322
Medvik
Zdroj

The Extended Low Temperature Method (ELTM) for the in-situ preparation of plant samples in an environmental scanning electron microscope enables carrying out repetitive topographical and material analysis at a higher resolution in the vacuum conditions of a scanning electron microscope or in the low gas pressure conditions of an environmental scanning electron microscope. The method does not require any chemical intervention and is thus suitable for imaging delicate structures rarely observable with common treatment methods. The method enables both sample stabilization as close to their native state as possible, as well as the transfer of the same sample from a low vacuum to an atmospheric condition for sample storage or later study. It is impossible for wet samples in the environmental scanning electron microscope. Our studies illustrate the high applicability of the ELTM for different types of plant tissue, from imaging of plant waxes at higher resolution, the morphological study of highly susceptible early somatic embryos to the elemental microanalysis of root cells. The method established here provides a very fast, universal and inexpensive solution for plant sample treatment usable in a commercial environmental scanning electron microscope equipped with a cooling Peltier stage.