loop test
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OBJECTIVES: The aim of the present study was to diagnose heart failure with preserved ejection fraction (HFPEF) in outpatients with unexplained chronic dyspnea and to elucidate its underlying mechanisms in this population using invasive pressure-volume loop analysis. BACKGROUND: The diagnosis of HFPEF in stable outpatients with unexplained dyspnea is difficult. METHODS: Thirty patients (age 67 +/- 8.6 years, 27% males) with preserved left ventricular (LV) ejection fraction (>50%) and unexplained chronic New York Heart Association functional class II to III dyspnea underwent heart catheterization. Patients with significant coronary artery stenosis (>50%) were excluded. Pressure-volume loops were assessed using a conductance catheter at rest, hand-grip exercise, leg lifting, and nitroprusside and dobutamine infusion. RESULTS: Twenty (66%) patients showed LV end-diastolic pressure >16 mm Hg (HFPEF), whereas the remaining 10 patients served as controls. Patients with HFPEF had significantly higher end-diastolic stiffness (0.205 +/- 0.074 vs. 0.102 +/- 0.017, p < 0.001) at rest, and their end-diastolic pressure-volume relationship showed a consistent upward and leftward shift during all hemodynamic interventions compared with controls. Regarding the underlying mechanism of HFPEF, 14 (70%) patients had markedly increased end-diastolic stiffness, which was considered a sufficient single pathology to induce increased LV end-diastolic pressure. Four (20%) patients showed a concomitant presence of moderately increased stiffness and severe LV dyssynchrony, and the remaining 2 (10%) patients, with normal stiffness, showed significant exercise-induced mitral regurgitation at hand-grip exercise. If the invasive pressure measurements were absent, only 5 (25%) of the outpatients with HFPEF fulfilled the European Society of Cardiology definition of HFPEF. CONCLUSIONS: A significant proportion of stable outpatients with unexplained chronic dyspnea may have HFPEF. In the patients whom we studied, increased LV stiffness, dyssynchrony, and dynamic mitral regurgitation were the major mechanisms underlying development of HFPEF. Copyright (c) 2010 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.
- MeSH
- diastola MeSH
- diferenciální diagnóza MeSH
- dyspnoe * diagnóza etiologie patofyziologie MeSH
- funkce levé komory srdeční * fyziologie MeSH
- komorový tlak (srdce) * fyziologie MeSH
- lidé MeSH
- následné studie MeSH
- pacienti ambulantní * MeSH
- progrese nemoci MeSH
- pulzní dopplerovská echokardiografie MeSH
- senioři MeSH
- srdeční katetrizace MeSH
- srdeční selhání diagnóza komplikace patofyziologie MeSH
- stupeň závažnosti nemoci MeSH
- tepový objem * fyziologie MeSH
- zátěžový test MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Implantabilní monitorovací jednotka (ILR) kontinuálně monitoruje EKG po dobu několika měsíců s možností telemetrické aktivace po proběhlé synkopě a registrací záznamu během symptomů. Cílem práce je zhodnotit přínos ILR k diagnóze původu synkopy v souboru 48 pacientů s rekurentní synkopou neobjasněnou konvenčními testy. Na základě dokumentovaného rytmu při recidivě synkopy u 34 pacientů (71 % souboru) po implantaci ILR byla synkopa arytmického původu u 13 pacientů (38 %), nearytmická v 21 případech (62 %). Užití ILR představuje efektivní metodu v diagnóze synkop neobjasněných konvenčními testy.
Conventional tests have known limitations in the diagnosis of syncope. Implantable loop recorder (ILR) is capable to monitor continously ECG over a period of several months with an option to record signals during a period of symptoms after post hoc telemetric activation. The aim of the study was to evaluate the significance of ILR in patients (pts) with recurrent syncope of unknown origin. ILR was implanted in 48 pts (29 female), mean age 51±17 years, (range 16-78 years) with unexplained syncope. A total of 21 pts (44%) had structural heart disease; none of them had significant left ventricular dysfunction. Of this study population 47 pts (98%) had previous negative 24-hour ECG monitoring (Holter), 38 pts (79%) had undergone previous negative head-up tilt test and 31 pts (65%) electrophysiological study with negative result. The mean follow-up was 6,4±4,2 months, (range 1-14 months). Syncope occurred in 34 pts (71%) during mean time 3,1±2,3 months, (range 1-10 months) after implantation. Significant arrhythmia was detected in 13 pts (38%): bradycardia in 5 pts, tachycardia in 8 pts. In 21 pts the recording did not explain syncope: 19 pts were in sinus rhythm, 2 pts had atrial fibrillation and syncope was classified as nonarrhythmic (62%). No complications were associated with the procedure. ILR is a useful diagnostic tool in syncopal patients with infrequent symptoms and negative results of previous conventional tests.
- MeSH
- defibrilátory implantabilní MeSH
- dospělí MeSH
- elektrokardiografie ambulantní metody MeSH
- lidé MeSH
- monitorování fyziologických funkcí metody MeSH
- srdeční arytmie patologie MeSH
- synkopa terapie MeSH
- technologie lékařská MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
Cieľom štúdie je overiť reliabilitu, konvergentnú a divergentnú validitu Pamäťového testu pre bilingválnu detskú populáciu s materinským jazykom maďarským (PTB). Štruktúra PTB a jeho administrácia vychádza z Pamäťového testu učenia, nie je však jeho prekladom. Obsahová validita testu bola overená expertmi. Výskumný súbor tvorilo 224 respondentov, 118 dievčat a 106 chlapcov s priemerným vekom 11,84 (SD = 1,93). Každý respondent bol testovaný individuálne nasledujúcimi výskumnými metódami: PTB, Re produkcia bezvýznamných slov (RBS) a Wechslerova inteligenčná škála (WISC IV). Reliabilita PTB vyjadrená koreláciou paralelných foriem testu je 0,79, hodnota Cronbachovej alphy je 0,7–0,77. Korelácia výkonu v PTB a RBS odrážajúca konvergentnú validitu je slabá až stredne silná. Medzi indexmi verbálneho porozumenia a pracovnej pamäti z WISC IV a PTB sa potvrdila iba veľmi slabá až slabá korelácia, poukazujúca na divergentnú validitu PTB. Reliabilita testu je dostatočná, kým konvergentná a divergentná validita čaká na opätovné overenie. Výsledky práce môžu tvoriť východiskový bod pre doplňujúce psychometrické postupy.
Objective: Despite the broad scientific interest in bilingual advantage/disadvantage in the domain of cognitive and executive functions, little attention is paid to the actual language of applied psychodiagnostic tools. However, the performance of bilingual clients may be lessened by the absence of match between their first language and the language of the psychological questionnaire. When bilinguals take verbal working memory tests in their second language, the operation of phonological loop is paired with language code switching, making the performance more difficult. In Slovakia, an estimated 9% of the population claim Hungarian to be their first language; however, adequate psychodiagnostic tools are not available for their psychological evaluation. The aim of the present study was to prepare a Memory test for bilingual children with Hungarian as their first language (PTB) and to verify its reliability, convergent and divergent validity.Methods: The PTB resembles the Auditory Verbal Learning test (Pamäťový test učenia) as it consists of 15 words for memorizing (set A) and 15 words for interference as well as 15 words for retest (set B). One has to memorize and recall set A for five times after it is read out by the administrator, then memorize and recall the words for inference (after being read out) and for the sixth time recall the words from set A without them being read out. Finally, after 30 minutes, one has to recall once again the words from set A without them being previously read out. The PTB is not a translated form of the Auditory Verbal Learning test. It consists of new, updated words frequently used by Slovak citizens with Hungarian as their first language in a specific geographical region. The content validity of the PTB was verified by experts. The research group comprised 224 respondents, 118 girls and 106 boys, with the average age of 11,84 (SD = 1,93). Each respondent was tested individually by the following research methods: the PTB, reproduction of meaningless words, and the Wechsler intelligence scale for children (WISC IV) in Hungarian. The reproduc tion of meaningless words requires the activity of phonological loop, being active also during memory tasks similar to the PTB. For this reason, a comparison of results obtained by these two methods can clarify the convergent validity of the PTB. While declaring discri minant validity we compared the achievement in the PTB with the verbal comprehension index and the working memory index from WISC IV. According to the factor analysis, verbal subtests such as similarities, vocabulary, information and digit span loaded a different factor from The Rey Auditory Verbal Learning Test that is very similar to the PTB.Results: The reliability of the PTB as a correlation between the two forms of tests is 0,79 (p=0,01), Cronbach’s alpha for set A is 0,7, while it is 0,77 for set B. Also, weak to moderate positive correlations (0,21–0,44; p=0,01) were found between the PTB and the reproduction of meaningless words; weak positive correlations were found between the PTB and the verbal comprehension index (0,06–0,18; p=0,01) and the working memory index (0,08 0,29; p=0,01).Discussion and conclusion: The obtained results of reliability are comparable to the reliability of The Rey Auditory Verbal Learning Test (0,6–0,77) and are considered sufficient. The rates of the other reliability indicators are acceptable; however, they are little lower than those obtained during The Rey Auditory Verbal Learning Test. The rate of correlation between the PTB and the reproduction of meaningless words indicating conver gent validity is acceptable as a significant difference being detected between the memorizing capacity and the accuracy of meaningful and meaningless words. Lack of correlation between the PTB and the verbal comprehension index is in line with the factor analysis of Ryan, Rosenberg and Mittenberg (1984), and thus ratifies the discriminant validity of the PTB. The weak correlation between the PTB and the working memory index may be unexpected as both measure hypothetically the same domains. However, in a spectre of research results, the ability of memorizing a digit span may differ from the ability of memorizing verbal spans. Indicators of reliability, convergent and divergent validity of the PTB are sufficient but further reassessment is needed.
- MeSH
- dítě MeSH
- jazyk (prostředek komunikace) MeSH
- lidé MeSH
- psychometrie MeSH
- testy paměti a učení * MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
A loop-mediated isothermal amplification (LAMP) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (PCV1) in commercial swine vaccines. This method was highly specific for PCV1. No cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. The analytical sensitivity of the LAMP for PCV1 DNA was 10 copies/μl in the case of positive recombinant plasmid comparable to that obtained from the nested polymerase chain reaction (nested PCR). Furthermore, 25 commercial swine vaccines were tested by both the LAMP and the nested PCR, and three of them were tested positive for PCV1 DNA. These results indicate that PCV1 DNA can be real-time detected by the LAMP; the method was highly specific, sensitive, and rapid for the detection of PCV1 DNA, particularly in commercial swine vaccines.
- MeSH
- Circovirus klasifikace genetika izolace a purifikace MeSH
- DNA primery genetika MeSH
- infekce viry čeledi Circoviridae prevence a kontrola veterinární virologie MeSH
- nemoci prasat prevence a kontrola virologie MeSH
- prasata MeSH
- techniky amplifikace nukleových kyselin metody MeSH
- virové vakcíny genetika izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
Interactions of colicins U and Y with the OmpA (Outer membrane protein A) receptor molecule were studied using site-directed mutagenesis and colicin binding assay. A systematic mutagenesis of the colicin-susceptible OmpA sequence from Escherichia coli (OmpAEC) to the colicin-resistant OmpA sequence from Serratia marcescens (OmpASM) was performed in regions corresponding to extracellular OmpA loops 1-4. Susceptibility to colicins U and Y was significantly affected by the OmpA mutation in loop 1. As with functional analysis, a decrease in binding capacity of His-tagged colicin U was found for recombinant OmpA with a mutated segment in loop 1 compared to control OmpAEC. To verify the importance of the identified amino acid residues in OmpA loop 1, we introduced loop 1 from OmpAEC into OmpASM, which resulted in the substantial increase of susceptibility to colicins U and Y. In addition, colicins U and Y were tested against a panel of 118 bacteriocin non-producing strains of four Escherichia species, including E. coli (39 strains), E. fergusonii (10 strains), E. hermannii (42 strains), and E. vulneris (27 strains). A majority (82%) of E. coli strains was susceptible to colicins U and Y. Interestingly, colicins U and Y also inhibited all of the 30 tested multidrug-resistant E. coli O25b-ST131 isolates. These findings, together with the fact that OmpA loop 1 is important for bacterial virulence and is evolutionary conserved, offer the potential of using colicins U and Y as specific anti-OmpA loop 1 directed antibacterial proteins.
- MeSH
- Escherichia coli účinky léků genetika růst a vývoj MeSH
- koliciny metabolismus MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mutační analýza DNA MeSH
- proteiny vnější bakteriální membrány genetika metabolismus MeSH
- Serratia marcescens genetika MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings.
- MeSH
- bakteriální proteiny genetika MeSH
- DNA bakterií genetika MeSH
- frambézie mikrobiologie MeSH
- fylogeneze MeSH
- lidé MeSH
- techniky amplifikace nukleových kyselin metody MeSH
- techniky typizace bakterií MeSH
- Treponema pallidum klasifikace genetika izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
Poultry Flavivirus (PF) was a recently emerged virus with high morbidity rates and mortality rates in China. It is the causative agent of egg drop syndrome at present. Development of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was the most efficient way to prevent and control the PF disease. The assay was performed at 64 °C for 45 min, using six specific primers that recognized eight targets of the PF E gene. The RT-LAMP assay, compared to conventional reverse transcription polymerase chain reaction, has 100-fold-greater sensitivity, with a detection limit of 1 × 10(-3) copies per μL RNA and no cross-reaction with poultry other viruses. The RT-LAMP assay is a valuable tool for detected PF without requiring any sophisticated equipment, and the detection has potential usefulness for clinical diagnosis in the field.
- MeSH
- diagnostické techniky molekulární metody MeSH
- DNA primery genetika MeSH
- drůbež MeSH
- Flavivirus genetika izolace a purifikace MeSH
- infekce viry z rodu Flavivirus diagnóza veterinární virologie MeSH
- nemoci drůbeže diagnóza virologie MeSH
- proteiny virového obalu genetika MeSH
- senzitivita a specificita MeSH
- techniky amplifikace nukleových kyselin metody MeSH
- veterinární lékařství metody MeSH
- virologie metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Čína MeSH
In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2–6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.
- MeSH
- lidé MeSH
- myši inbrední ICR MeSH
- myši MeSH
- protozoální DNA genetika moč MeSH
- techniky amplifikace nukleových kyselin metody MeSH
- Toxoplasma genetika izolace a purifikace MeSH
- toxoplazmóza diagnóza parazitologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
