BACKGROUND: Auer rods (AuRs) are prominent intracellular structures found almost exclusively in myeloid cell malignancies, such as acute myeloid leukemia (AML), chronic and juvenile myelomonocytic leukemia and myelodysplastic syndrome. Extremely rare AuRs have been reported in patients with acute lymphoblastic leukemia (ALL) or among ambiguous lineage leukemia patients with a dominantly lymphoblastic immunophenotype. PROCEDURE: We report diagnostic and follow-up data of an international cohort of 11 children suffering from leukemias with AuRs and with significant presence of T and myeloid markers, majority of whom categorized as early T-cell precursor (ETP, n = 7); or T-ALL (ETP status unknown, n = 2), ALAL (acute leukemia of ambiguous lineage, n = 1), and AML reclassified from ALAL (n = 1). We described other diagnostic details and treatment types and responses. Moreover, we summarize previously published data. RESULTS: Among the four patients who started and remained on ALL-type therapy, all were in the first complete remission, whereas both patients who started and remained on AML-type therapy relapsed and died. Of the patients who followed either a combined ALL/AML protocol (Interfant 06) or who switched from one of the two types of therapy to the other, one patient died, and the remaining four were in first complete remission at the most recent follow-up. We also searched for similar cases in the literature and found only three additional children with nonmyeloid leukemia and AuRs and 10 adults with this type of leukemia. CONCLUSIONS: Briefly, ALL- or combined ALL/AML-type therapy may be effective for treating AuR-positive leukemia patients with a lymphoid immunophenotype.

• MeSH
• akutní lymfatická leukemie patologie   terapie   imunologie   MeSH
• akutní myeloidní leukemie patologie   terapie   imunologie   MeSH
• dítě MeSH
• imunofenotypizace * MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• následné studie MeSH
• předškolní dítě MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: A number of recent studies have shown that the intestinal microbiome, part of the brain-gut axis, is implicated in the pathophysiology of multiple sclerosis. An essential part of this axis, is the intestinal barrier and gastrointestinal disorders with intestinal barrier dysregulation appear to be linked to CNS demyelination, and hence involved in the etiopathogenesis of multiple sclerosis (MS). OBJECTIVE: The aim of this study was to evaluate the integrity of the intestinal barrier in patients with clinically definite multiple sclerosis (CDMS) and clinically isolated syndrome (CIS) using two serum biomarkers, claudin-3 (CLDN3), a component of tight epithelial junctions, and intestinal fatty acid binding protein (I-FABP), a cytosolic protein in enterocytes. METHODS: Serum levels of CLDN3 in 37 MS patients and 22 controls, and serum levels of I-FABP in 46 MS patients and 51 controls were measured using commercial ELISA kits. Complete laboratory tests excluded the presence of gluten-related disorders in all subjects. Thirty MS patients received either disease-modifying drugs (DMD), immunosuppression (IS) or corticosteroid treatment. RESULTS: CLDN3 levels were only significantly higher in the MS patients treated with DMD or IS compared to the control group (P=0.006). There were no differences in I-FABP serum levels between the groups. Serum CLDN3 levels did not correlate with serum I-FABP levels in CDMS, in CIS patients or controls. CONCLUSIONS: In multiple sclerosis patients, the intestinal epithelium may be impaired with increased permeability, but without significant enterocyte damage characterized by intracellular protein leakage. Based on our data, CLDN3 serum levels appear to assess intestinal dysfunction in MS patients but mainly in treated ones.

• MeSH
• biologické markery * krev   MeSH
• claudin-3 * metabolismus   MeSH
• dospělí MeSH
• funkce střevní bariéry MeSH
• lidé středního věku MeSH
• lidé MeSH
• permeabilita * MeSH
• proteiny vázající mastné kyseliny * krev   MeSH
• roztroušená skleróza * patofyziologie   metabolismus   krev   MeSH
• střevní sliznice metabolismus   MeSH
• studie případů a kontrol MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Cíl: Prezentovat ojedinělý případ náhodné detekce nádorové kvadruplicity (prostata, tračník, trachea) pomocí hybridního 18 F-FDG PET/CT zobrazení u 74letého muže s pozitivní anamnézou bronchioloalveolárního karcinomu. Metodika: PET/CT vyšetření bylo provedeno za 60 minut po intravenózní aplikaci 18 F-FDG o aktivitě 4,5 MBq/kg. V identickém rozsahu bylo provedeno též monofázické CT zobrazení, s předcházejícím podáním per os kontrastní látky a intravenózní aplikací bolusu jodové kontrastní látky. Výsledky: Zobrazilo se ložisko akumulace 18 F-FDG v distální části trachey, v lymfatické uzlině na krku vlevo, dále excentrické zesílení stěny esovitého tračníku s akumulací 18 F-FDG a ložisko zvýšené akumulace 18 F-FDG v periferii pravého laloku prostaty. Nález byl hodnocen jako viabilní metastáza na krku a v distální části trachey a velmi suspektní podezření na synchronní tumor tračníku a prostaty. Bronchoskopie s biopsií prokázala dlaždicobuněčný karcinom v průdušnici. Pacient také absolvoval koloskopii s biopsií, která potvrdila přítomnost adenokarcinomu v tračníku. Při následné operaci se zjistilo, že tumor tračníku je inoperabilní, prorůstá do močového měchýře a byla založena kolostomie. Při vyšetření sérové hladiny PSA byla zjištěna elevace (6,5 μg/l; norma 0–4 μg/l), biopsie potvrdila přítomnost karcinomu prostaty. Vzhledem ke klinickému stavu pacienta a jeho přání byla poté ukončena kurativní terapie a dále pokračovala pouze paliativní symptomatická léčba. Závěr: 18 F-FDG PET/CT vyšetření správně náhodně detekovalo nádorovou kvadruplicitu (tračník, prostata, průdušnice), což zcela změnilo prognózu pacienta a v podstatě i strategii léčby. PET/CT vyšetření významně zkrátilo dobu diagnostického procesu.

Aim: A rare case of incidental detection of quadruple primary cancer (colon, prostate and trachea) by means of 18 F-FDG PET/CT in a 74-year-old man with positive history of bronchioalveolar carcinoma. Method: PET/CT scan was performed in 60 minutes after intravenous administration of 18 F-FDG with activity of 4.5 MBq/kg. Monophasic contrast-enhanced CT imaging was performed in identical extent. Acquisition of CT imaging data was targeted to the venous circulation. Results: A focus of increased 18 F-FDG uptake in the left neck lymph node and the distal part of the trachea, as well as eccentric thickening of the wall of the sigmoid colon with 18 F- FDG uptake and a focus of increased 18 F-FDG uptake in the periphery of the right lobe of the prostate were detected. The finding was evaluated as a viable metastasis to the neck and distal trachea and highly suspicious for a synchronous tumour of the sigmoid colon and prostate. Bronchoscopy with biopsy showed squamous cell carcinoma in the trachea. The patient also underwent colonoscopy and biopsy, which confirmed the presence of adenocarcinoma in the colon. Abdomen surgery revealed locally advanced, inoperable tumour and a colostomy was established. Serum PSA levels were found to be increased (6.5 μg/l; normal 0–4 μg/l) and biopsy confirmed the presence of prostate cancer. Due to the patient‘s clinical condition and wishes, curative therapy was then discontinued, and only palliative symptomatic treatment was continued. Conclusion: The 18 F-FDG PET/CT scan correctly detected tumour quadruple (colon, prostate, trachea) in a patient with a positive history of bronchioalveolar carcinoma, which completely changed the prognosis of the patient and essentially the treatment strategy. Furthermore, PET/CT scanning significantly reduced the diagnostic process time.

Léčebny dlouhodobě nemocných jsou typem zdravotnického zařízení, které je definováno nejen legislativou, ale především specifickými léčebnými postupy. Diagnostické možnosti jsou limitované a na léčebný tým jsou kladeny především nároky na zkušenost a komplexní znalosti mnoha lékařských oborů. Měření výdeje tekutin, posouzení jejího vzhledu, změny objemu a charakteru v čase u polymorbidních nemocných s polypragmazií je jeden z mála postupů, jehož data mohou mít lékaři následné péče k dispozici. Vedle skupiny nemocných s patologií ledvin neschopných objektivizovat výdej tekutin jinak, je indikací přechodného zavedení permanentního močového katétru snaha zabránit infikování proleženin nebo intertriga, léčba infekcí vyžadující derivaci dolních močových cest, imobilita nemocných s anatomickou nebo funkční patologií dolních močových cest bránící fyziologické evakuaci močového měchýře, včetně poruch vědomí. Imperativní indikací je retence moče pro subvezikální obstrukci. Každá jiná indikace je mimo evropská i severoamerická doporučení, neboť na jakýkoliv způsob zevní derivace moče je nahlíženo jako na instrumentaci s významným rizikovým potenciálem vzniku infekce močových cest dominantně nemocničními multirezistentními bakteriálními kmeny (Catheter­‐Associated Urinary Tract Infection – CAUTI). Při fyziologických změnách imunity u seniorů, násobené přítomností mnoha život ohrožujících diagnóz, jsou CAUTI u 40 % zemřelých v přímé příčinné souvislosti. Přes lege artis indikace se počet nemocných s trvalou derivací dolních močových cest permanentním močovým katétrem na českých pracovištích následné péče jeví výrazně vyšší, než tomu je v zahraničí na srovnatelných pracovištích. Hodnotitelná data z České republiky však chybí. Problematika nezbytnosti zavedení permanentního močového katétru, profit nemocných s permanentním močovým katétrem je důležitou otázkou, kterou si musíme, nejen v následné dlouhodobé péči, pokládat.

Long-term care hospitals are a type of healthcare facility that is defined not only by legislation, but above all by specific treatment procedures. Diagnostic possibilities are limited and the treatment team is mainly required to have experience and comprehensive knowledge of many medical disciplines. Measuring fluid output, assessing its appearance, volume change and character over time in polymorbid patients with polypragmasia is one of the few procedures whose data may be available to follow-up care physicians. In addition to the group of patients with renal pathology unable to objectify fluid output in any other way, the indications for temporary placement of a indwelling urinary catheter include efforts to prevent infection of bedsores or interstitium, treatment of infections requiring lower urinary tract drainage, immobility of patients with anatomic or functional lower urinary tract pathology preventing physiologic evacuation of the bladder, including disturbances of consciousness. Urinary retention due to subvesical obstruction is an imperative indication. Any other indication is outside of European and North American recommendations, as any method of external urinary diversion is viewed as instrumentation with significant risk potential for urinary tract infection with predominantly hospital-acquired multidrug-resistant bacterial strains (Catheter-Associated Urinary Tract Infection (CAUTI).With physiological changes in immunity in the elderly, multiplied by the presence of many life-threatening diagnoses, CAUTIs are directly causal in 40% of deaths. Despite the lege artis indications, the number of patients with permanent lower urinary tract catheter derivation in Czech aftercare units appears to be significantly higher than in comparable units abroad. However, evaluable data from the Czech Republic are lacking. The issue of the necessity of permanent urinary catheter placement and the profit of patients with permanent urinary catheter placement is an important question that must be asked, not only in long-term follow-up care.

BACKGROUND: In our previous study on erythropoiesis-stimulating agent (ESA) treatment in lower risk myelodysplastic syndromes from the European MDS (EUMDS) Registry, we showed that patients treated with ESAs had longer survival compared with patients who receive red blood cell transfusion (RBCT). In this study, with a longer follow up time and more patients included, we aimed to assess long-term effects on survival and health-related quality of life (HRQoL) of exposure to ESAs with or without RBCT in patients with lower risk myelodysplastic syndromes. METHODS: The EUMDS Registry is a non-interventional, longitudinal, real-world registry prospectively enrolling newly diagnosed patients older than 18 years with lower risk (International Prognostic Scoring System low or intermediate-1) myelodysplastic syndromes from 16 European countries and Israel. The analysis was restricted to patients with haemoglobin concentrations less than 100 g/L enrolled between Jan 1, 2008, and July 1, 2019, with last censoring of data on Dec 31, 2021. Patient management was recorded every 6 months, including treatment, transfusions, and HRQoL. ESA treatment followed local guidelines. The patients were separated into four groups at each study visit: no ESA or RBCT, ESA only, ESA plus RBCT, and RBCT only. The data were analysed longitudinally over time according to ESA and RBCT status during each 6-month interval, using propensity score matching. The main outcomes were median overall survival and leukaemia-free survival, and HRQoL. This study is registered with ClinicalTrials.gov, NCT00600860, as is ongoing. FINDINGS: 2448 patients (the ESA-unexposed group [n=1265] and ESA-exposed group [n=1183]) were diagnosed before July 1, 2019; 1520 (62·1%) were male and 928 (37·9%) were female. Median follow-up time was 3·9 years (IQR 1·6-6·5). After applying eligibility criteria and propensity matching, there were 426 patients in the ESA-unexposed group and 744 patients in the ESA-exposed group. Median overall survival in the ESA exposed group was 44·9 months (95% CI 40·2-50·5) compared with 34·8 months (28·6-39·2) in the ESA unexposed group; the absolute difference was 10·1 months (95% CI 2·2-18·0; hazard ratio [HR] 0·70 [95% CI 0·59-0·83]; p<0·0001). Patients without RBCT in the presence or absence of ESA exposure maintained significantly better HRQoL than those with RBCT, irrespective of ESA exposure (linear mixed effect model of EQ-5d-3L index score, RBCT coefficient -0·04 [95% CI -0·06 to 0·03], p<0·0001; linear mixed effect model of VAS, -4·57 [-6·02 to -3·13], p<0·0001). INTERPRETATION: ESA treatment in patients with lower risk myelodysplastic syndromes significantly improves overall survival when started before or early after the onset of regular transfusion therapy. Avoiding RBCT is associated with significantly better HRQoL. FUNDING: H2020 European Research Council, Novartis Pharmacy B V Oncology Europe, Amgen, BMS/Celgene International, Janssen Pharmaceutica, Takeda Pharmaceuticals International, and Gilead Sciences.

• MeSH
• hematinika * terapeutické užití   MeSH
• kohortové studie MeSH
• kvalita života * MeSH
• lidé středního věku MeSH
• lidé MeSH
• longitudinální studie MeSH
• myelodysplastické syndromy * farmakoterapie   mortalita   terapie   komplikace   MeSH
• registrace MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• transfuze erytrocytů škodlivé účinky   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• pozorovací studie MeSH

INTRODUCTION: Moderate and high levels of anticardiolipin antibodies (aCL), especially in the setting of the antiphospholipid syndrome, are associated with adverse obstetric outcomes. However, the clinical relevance of low aCL levels (<40 MPL/GPL units) is still a matter of debate. The aim of the study was to evaluate obstetric outcomes in pregnancies with low immunoglobulin M (IgM) and/or immunoglobulin G (IgG) aCL positivity. The association between low aCL positivity and maternal baseline characteristics was also studied. MATERIAL AND METHODS: The retrospective monocentric cohort study of prospectively collected data involved a total 3047 singleton pregnancies that underwent the first-trimester screening involving an aCL test and delivered on site. Obstetric outcomes were compared between the low-titer aCL group (IgM ≥7 MPL units and <40 MPL units and/or IgG ≥10 GPL units and <40 GPL units) and the aCL negative group (IgM <7 MPL units and IgG <10 GPL units, reference group). In addition, obstetric outcomes were evaluated with regard to the antibody isotype: IgM-positive group (IgM <40 MPL units, IgG negative) and IgG-positive group (IgG <40 GPL units, IgM negative or <40 MPL units). RESULTS: Overall, the occurrence of pregnancy-related complications was significantly higher (27.91% vs. 19.32%, p = 0.034) in the low-titer aCL group. Concerning the antibody isotype, a higher rate of pregnancy-related complications was observed in the IgG-positive group (54.55% vs. 19.32%, p = 0.001), but not in the IgM-positive group (22.43% vs. 19.32%, p = 0.454). The stillbirth rate did not reach statistical significance. Low-titer aCL pregnancies were more frequently of advanced maternal age (p < 0.001), suffered from autoimmune diseases (p < 0.001), chronic hypertension (p = 0.040), and hereditary thrombophilia (p = 0.040). In addition, they had more often a positive history of stillbirth (p < 0.001), underwent conception via assisted reproductive technologies (p < 0.001), were administered low-dose aspirin (p < 0.001), low-molecular-weight heparin (p = 0.018) and immunomodulatory drugs (p < 0.001), and delivered earlier (p = 0.018). CONCLUSIONS: Even low aCL levels are associated with a higher incidence of pregnancy-related complications, but only in the case of IgG antibody isotype presence. Screening for aCL in the first trimester has some prognostic value, but further studies are needed to determine whether its potential implementation into routine clinical practice would improve antenatal care.

• MeSH
• antifosfolipidový syndrom krev   MeSH
• antikardiolipinové protilátky * krev   MeSH
• dospělí MeSH
• imunoglobulin G krev   MeSH
• imunoglobulin M krev   MeSH
• kohortové studie MeSH
• komplikace těhotenství * krev   imunologie   epidemiologie   MeSH
• lidé MeSH
• retrospektivní studie MeSH
• těhotenství MeSH
• výsledek těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The exon junction complex (EJC) is a key player in metazoan mRNA quality control and is placed upstream of the exon-exon junction after splicing. Its inner core is composed of Magoh, Y14, eIF4AIII and BTZ and the outer core of proteins involved in mRNA splicing (CWC22), export (Yra1), translation (PYM) and nonsense mediated decay (NMD, UPF1/2/3). Trypanosoma brucei encodes only two genes with introns, but all mRNAs are processed by trans-splicing. The presence of three core EJC proteins and a potential BTZ homologue (Rbp25) in trypanosomes has been suggested to adapt of the EJC function to mark trans-spliced mRNAs. We analysed trypanosome EJC components and noticed major differences between eIF4AIII and Magoh/Y14: (i) whilst eIF4AIII is essential, knocking out both Magoh and Y14 elicits only a mild growth phenotype (ii) eIF4AIII localization is mostly nucleolar, while Magoh and Y14 are nucleolar and nucleoplasmic but excluded from the cytoplasm (iii) eIF4AIII associates with nucleolar proteins and the splicing factor CWC22, but not with Y14 or Magoh, while Magoh and Y14 associate with each other, but not with eIF4AIII, CWC22 or nucleolar proteins. Our data argue against the presence of a functional EJC in trypanosomes, but indicate that eIF4AIII adopted non-EJC related, essential functions, while Magoh and Y14 became redundant. Trypanosomes also possess homologues to the NMD proteins UPF1 and UPF2. Depletion of UPF1 causes only a minor reduction in growth and phylogenetic analyses show several independent losses of UPF1 and UPF2, as well as complete loss of UPF3 in the Kinetoplastida group, indicating that UPF1-dependent NMD is not essential. Regardless, we demonstrate that UPF1 depletion restores the mRNA levels of a PTC reporter. Altogether, we show that the almost intron-less trypanosomes are in the process of losing the canonical EJC/NMD pathways: Y14 and Magoh have become redundant and the still-functional UPF1-dependent NMD pathway is not essential.

• MeSH
• eukaryotický iniciační faktor 4A metabolismus   genetika   MeSH
• exony genetika   MeSH
• messenger RNA genetika   metabolismus   MeSH
• nonsense mediated mRNA decay * MeSH
• protozoální proteiny * metabolismus   genetika   MeSH
• sestřih RNA MeSH
• Trypanosoma brucei brucei * metabolismus   genetika   MeSH
• Publikační typ
• časopisecké články MeSH

Aim: The study aimed to assess the prevalence of birth trauma in postpartum women and to identify factors that may influence the development of birth trauma. Methods: The sample consisted of 238 women at 6-12 weeks postpartum. Data were collected using the City Birth Trauma Scale (CityBiTS), a standardized tool that identifies a woman's potentially traumatic experiences during or after her last childbirth. The questionnaire was supplemented with sociodemographic data. Results: Nearly 11% of participants experienced some form of post-traumatic stress disorder (PTSD) after childbirth. The most intense symptoms were those belonging to the hyperarousal subscale, especially feelings of nervousness, tension, irritability, and aggression. Statistically significant differences in the prevalence of birth trauma were found with regard to the presence of a birth support person (p = 0.044) and week of gestation at the time of delivery (p = 0.001). Differences related to participants' age, education, and type of delivery were not statistically significant. Conclusion: Our findings suggest that participants only experienced birth trauma symptoms to a small extent. The CityBiTS is a useful tool for measuring postpartum trauma. Women identified as having some form of PTSD should be referred for further psychological testing and psychological care.

• MeSH
• hodnocení rizik metody   MeSH
• lidé MeSH
• poporodní období psychologie   MeSH
• porod * psychologie   MeSH
• posttraumatická stresová porucha * diagnóza   epidemiologie   etiologie   MeSH
• průřezové studie MeSH
• průzkumy a dotazníky MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Geografické názvy
• Česká republika MeSH

Ticks are important vectors of various microorganisms, including bacteria. In this study, we examined Hyalomma aegyptium ticks collected from 240 spur-thighed tortoises Testudo graeca at 42 localities in the Mediterranean and Middle East and analysed them for the presence of bacteria of the genera Anaplasma, Borrelia, Coxiella, and Rickettsia. Altogether, 576 out of 928 analysed ticks (62.1%) were positive for at least one of the tested bacteria. The highest prevalence in individual ticks was found for Borrelia turcica (43.6%), followed by Rickettsia (12.3%) and Anaplasma (6.1%). No sample was positive for Coxiella burnetii. Among Rickettsia, we detected two species, Rickettsia africae and Rickettsia aeschlimannii, and also other unspecified Rickettsia. Anaplasma (100% identity with A. phagocytophilum) was detected at 15 (35%) out of 42 studied localities, any of Rickettsia at 28 (67%), and B. turcica at 32 (76%) localities. The geographic distribution of the studied microorganisms varied, with none of them detected in Syria, and only Rickettsia spp. detected in Morocco. Sequence analysis revealed substantial genetic variability in all detected agents, with the most variable (36 new haplotypes) being glpQ gene used as a marker for B. turcica. We also analysed the prevalence of various co-infections among studied ticks, with the mean number of co-infected ticks per tortoise increased with the number of ticks per tortoise. However, the frequencies of co-infected ticks do not indicate the presence of antagonistic or synergistic facilitative interactions between the agents. According to our data, we could expect that the eco-epidemiological importance of H. aegyptium does not stem from their tortoise hosts but rather from the low host specificity of its larvae and nymphs, feeding on a wider spectrum of reptilian, avian, and mammalian hosts.

• MeSH
• Anaplasma * izolace a purifikace   MeSH
• Borrelia izolace a purifikace   MeSH
• Coxiella izolace a purifikace   genetika   MeSH
• infestace klíšťaty veterinární   epidemiologie   parazitologie   MeSH
• Ixodidae * mikrobiologie   růst a vývoj   MeSH
• Rickettsia * izolace a purifikace   MeSH
• želvy * mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Střední východ MeSH
• Středomoří MeSH

Urinary tract infections (UTIs) are one of the most common bacterial diseases both in communities and in hospitalized patients, and at the same, time they are one of the most common indications for the use of antibiotics. UTI guidelines are generally available nationally or internationally, but they do not address all aspects of UTI treatment for different patient cohorts, age, gender, or comorbidities. The aim of the study was to point out the importance of stratified cumulative antibiograms at the level of individual health care facilities and the significant differences between epidemiological data, not only at the national level, but also at the local level. Our study analyses data from 383 patients with UTIs from a hospital department, General University Hospital (GUH), and 272 patients from an outpatient medical facility, Urocentrum (UC). This analysis focuses on the most common UTI causative agent, Escherichia coli, its representation as the causative agent of UTI in patients with complicated acute cystitis (N30), and its representation in complicated acute cystitis in patients with prostate cancer (C61). In addition to the frequency of occurrence, a sub-analysis of the incidence of resistance of E. coli to commonly used antibiotics by age, gender, diagnosis, and medical facility was performed. Results: The most common causative agent of UTI was E. coli. In patients with N30, it was 70% in GUH and 54% in UC, but in oncological patients with UTI, it was only 39% and 35%, respectively. In patients with UTI in C61, there was a significant difference in susceptibility of E. coli between individual health care facilities. Lower resistance was found in UC opposite to GUH isolates in ampicillin, with 29.8% vs. 65%, p = 0.001; amoxicillin/clavulanic acid, with 8.5% vs. 30%, p = 0.01; with 2.1% vs. 17.5% in pivmecillinam, p = 0.01; with 10.6% vs. 37.5% in co-trimoxazole, p = 0.003; and ciprofloxacin, with 10.6% vs. 30%, p = 0.04. The study shows significant differences in the sensitivity of urinary E. coli isolates in patients in relation to age, gender, medical devices, and the presence of comorbidities.

• MeSH
• antibakteriální látky * terapeutické užití   MeSH
• bakteriální léková rezistence MeSH
• dospělí MeSH
• Escherichia coli * účinky léků   izolace a purifikace   MeSH
• infekce močového ústrojí * farmakoterapie   mikrobiologie   epidemiologie   MeSH
• infekce vyvolané Escherichia coli farmakoterapie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mikrobiální testy citlivosti * MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH