Improper use of nicosulfuron (NSF) may induce harmful effects on plants during weed control. Melatonin (MT) regulates photosynthetic and physiological processes in plants. This study aimed to explore the effects of MT on alleviating NSF toxicity by measuring the growth parameters, photosynthetic capacity, and antioxidative responses in sweet corn seedlings. Compared to NSF alone, exogenous MT increased chlorophyll content, transpiration rate, net photosynthetic rate, stomatal conductance, and maximum efficiency of PSII photochemistry, while reduced malondialdehyde, hydrogen peroxide, superoxide anion radical, and proline contents. Moreover, MT also increased the activity of ascorbate peroxidase and the expression levels of ZmAPX1, ZmAPX2, ZmALS1, and ZmCYP81A9. The inhibition of p-chlorophenylalanine inhibited the positive effects of MT on photosynthetic and physiological indexes. The results indicated that pretreatment with MT might effectively mitigate NSF toxicity in sweet corn seedlings.

• Klíčová slova
• antioxidative system, herbicide stress, melatonin, photosystem, sweet corn seedlings,
• Publikační typ
• časopisecké články MeSH

Exogenously applied brassinosteroids (BRs) improve plant response to drought. However, many important aspects of this process, such as the potential differences caused by different developmental stages of analyzed organs at the beginning of drought, or by BR application before or during drought, remain still unexplored. The same applies for the response of different endogenous BRs belonging to the C27, C28-and C29- structural groups to drought and/or exogenous BRs. This study examines the physiological response of two different leaves (younger and older) of maize plants exposed to drought and treated with 24-epibrassinolide (epiBL), together with the contents of several C27, C28-and C29-BRs. Two timepoints of epiBL application (prior to and during drought) were utilized to ascertain how this could affect plant drought response and the contents of endogenous BRs. Marked differences in the contents of individual BRs between younger and older maize leaves were found: the younger leaves diverted their BR biosynthesis from C28-BRs to C29-BRs, probably at the very early biosynthetic steps, as the levels of C28-BR precursors were very low in these leaves, whereas C29-BR levels vere extremely high. Drought also apparently negatively affected contents of C28-BRs (particularly in the older leaves) and C29-BRs (particularly in the younger leaves) but not C27-BRs. The response of these two types of leaves to the combination of drought exposure and the application of exogenous epiBL differed in some aspects. The older leaves showed accelerated senescence under such conditions reflected in their reduced chlorophyll content and diminished efficiency of the primary photosynthetic processes. In contrast, the younger leaves of well-watered plants showed at first a reduction of proline levels in response to epiBL treatment, whereas in drought-stressed, epiBL pre-treated plants they were subsequently characterized by elevated amounts of proline. The contents of C29- and C27-BRs in plants treated with exogenous epiBL depended on the length of time between this treatment and the BR analysis regardless of plant water supply; they were more pronounced in plants subjected to the later epiBL treatment. The application of epiBL before or during drought did not result in any differences of plant response to this stressor.

• Klíčová slova
• OJIP analysis, brassinosteroids, drought, endogenous content, exogenous application, leaf age, proline,
• Publikační typ
• časopisecké články MeSH

Salinity is the primary environmental stress that adversely affects plants' growth and productivity in many areas of the world. Published research validated the role of aspartic acid in improving plant tolerance against salinity stress. Therefore, in the present work, factorial pot trials in a completely randomized design were conducted to examine the potential role of exogenous application of aspartic acid (Asp) in increasing the tolerance of wheat (Triticum aestivum L.) plants against salt stress. Wheat plants were sown with different levels of salinity (0, 30, or 60 mM NaCl) and treated with three levels of exogenous application of foliar spray of aspartic acid (Asp) (0, 0.4, 0.6, or 0.8 mM). Results of the study indicated that salinity stress decreased growth attributes like shoot length, leaf area, and shoot biomass along with photosynthesis pigments and endogenous indole acetic acid. NaCl stress reduced the total content of carbohydrates, flavonoid, beta carotene, lycopene, and free radical scavenging activity (DPPH%). However, Asp application enhanced photosynthetic pigments and endogenous indole acetic acid, consequently improving plant leaf area, leading to higher biomass dry weight either under salt-stressed or non-stressed plants. Exogenous application of Asp, up-regulate the antioxidant system viz. antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and nitrate reductase), and non-enzymatic antioxidants (ascorbate, glutathione, total phenolic content, total flavonoid content, beta carotene, lycopene) contents resulted in declined in reactive oxygen species (ROS). The decreased ROS in Asp-treated plants resulted in reduced hydrogen peroxide, lipid peroxidation (MDA), and aldehyde under salt or non-salt stress conditions. Furthermore, Asp foliar application increased compatible solute accumulation (amino acids, proline, total soluble sugar, and total carbohydrates) and increased radical scavenging activity of DPPH and enzymatic ABTS. Results revealed that the quadratic regression model explained 100% of the shoot dry weight (SDW) yield variation. With an increase in Asp application level by 1.0 mM, the SDW was projected to upsurge through 956 mg/plant. In the quadratic curve model, if Asp is applied at a level of 0.95 mM, the SDW is probably 2.13 g plant-1. This study concluded that the exogenous application of aspartic acid mitigated the adverse effect of salt stress damage on wheat plants and provided economic benefits.

• Klíčová slova
• Antioxidant enzymes, Triticum aestivum, aspartic acid, hydrogen peroxide, lipid peroxidation, osmoprotectants, salinity stress,
• Publikační typ
• časopisecké články MeSH

In this study, we observed the effect of the application of soil dust enriched with risk elements (Cd, Pb, As and Zn) to leaf surfaces of lettuce (Lactuca sativa var. capitata) while it was grown under hydroponic conditions. This study aimed to determine how low soil dust particulate matter (PM) doses affected the activity of or damaged the photosynthetic apparatus and how the uptake of risk elements was associated with both epigenetic changes (5-methylcytosine content, i.e., 5mC) and stress metabolism. During the study, we obtained many results pertaining to risk element contents and biochemical (total phenolic content (TPC), malondialdehyde (MDA) content and the amount of free amino acids (AAs)) and physiological (photosynthesis parameters: net photosynthetic rate, transpiration rate, intercellular CO2 concentration, stomatal conductance, instantaneous water-use efficiency, maximum quantum yield of PSII, chlorophyll and carotenoid contents, and leaf water potential (WP)) plant features. The results showed an increase in MDA and 5mC. However, the transpiration rate, WP and free AAs decreased. In conclusion, contamination by very low doses of soil dust PM had no direct or significant effect on plant fitness, as shown by the TPC and 5mC content, which indicates that plants can overcome the oxidative stress caused by the accumulation of risk elements. From the above, we propose the use of epigenetic changes as biomarkers of potential changes in the activation of plant metabolism under stress caused by environmental pollution.

• MeSH
• chlorofyl metabolismus   MeSH
• fotosyntéza MeSH
• listy rostlin metabolismus   MeSH
• pevné částice * metabolismus   MeSH
• prach * MeSH
• půda chemie   MeSH
• rostliny metabolismus   MeSH
• salát (hlávkový) metabolismus   MeSH
• voda metabolismus   MeSH
• znečištění životního prostředí MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chlorofyl MeSH
• pevné částice * MeSH
• prach * MeSH
• půda MeSH
• voda MeSH

The ability to modulate photosynthesis is essential for plants to adapt to fluctuating growing conditions. Populus species show high tolerance to various and highly variable environments. To understand their response strategies against fluctuating environments, this study investigated the morphological and physiological differences of white poplar (Populus alba) leaves when grown in a phytotron, glasshouse, and field. Our results show that the palisade cells were elongated in the field, which would enhance intercellular CO2 exchange. Photosynthetic capacity was the highest in the field leaves, as shown by higher electron transport rates (1.8 to 6.5 times) and carbon assimilation rates (2.7 to 4.2 times). The decrease of PSI acceptor-side limitation and increase of PSI donor-side limitation suggests changes in PSI redox status may contribute to photoprotection. This plasticity of white poplar allows adjusting its structure and photosynthesis under fluctuating conditions, which may partly enable its outstanding tolerance against environmental changes.

• Klíčová slova
• Populus alba, fluctuating conditions, photoinhibition, photosynthesis, plasticity,
• Publikační typ
• časopisecké články MeSH

Sugar-mediated osmotic acclimation and a strong antioxidative response reduce drought-induced biomass loss at the vegetative stage in rice. A clear understanding of the physiological and biochemical adaptations to water limitation in upland and aerobic rice can help to identify the mechanisms underlying their tolerance to low water availability. In this study, three indica rice varieties-IR64 (lowland), Apo (aerobic), and UPL Ri-7 (upland)-, that are characterized by contrasting levels of drought tolerance, were exposed to drought at the vegetative stage. Drought-induced changes in biomass, leaf metabolites and oxidative stress markers/enzyme activities were analyzed in each variety at multiple time points. The two drought-tolerant varieties, Apo and UPL Ri-7 displayed a reduced water use in contrast to the susceptible variety IR64 that displayed high water consumption and consequent strong leaf dehydration upon drought treatment. A sugar-mediated osmotic acclimation in UPL Ri-7 and a strong antioxidative response in Apo were both effective in limiting the drought-induced biomass loss in these two varieties, while biomass loss was high in IR64, also after recovery. A qualitative comparison of these results with the ones of a similar experiment conducted in the field at the reproductive stage showed that only Apo, which also in this stage showed the highest antioxidant power, was able to maintain a stable grain yield under stress. Our results show that different metabolic and antioxidant adaptations confer drought tolerance to aerobic and upland rice varieties in the vegetative stage. The effectiveness of these adaptations differs between developmental stages. Unraveling the genetic control of these mechanisms might be exploited in breeding for new rice varieties adapted to water-limited environments.

• Klíčová slova
• Antioxidative response, Biomass, Drought, Metabolism, Oryza sativa, Osmotic adjustment, Vegetative stage,
• MeSH
• antioxidancia MeSH
• fyziologická adaptace MeSH
• období sucha MeSH
• rýže (rod) * MeSH
• šlechtění rostlin MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH

Plant survival in temperate zones requires efficient cold acclimation, which is strongly affected by light and temperature signal crosstalk, which converge in modulation of hormonal responses. Cold under low light conditions affected Arabidopsis responses predominantly in apices, possibly because energy supplies were too limited for requirements of these meristematic tissues, despite a relatively high steady-state quantum yield. Comparing cold responses at optimal light intensity and low light, we found activation of similar defence mechanisms-apart from CBF1-3 and CRF3-4 pathways, also transient stimulation of cytokinin type-A response regulators, accompanied by fast transient increase of trans-zeatin in roots. Upregulated expression of components of strigolactone (and karrikin) signalling pathway indicated involvement of these phytohormones in cold responses. Impaired response of phyA, phyB, cry1 and cry2 mutants reflected participation of these photoreceptors in acquiring freezing tolerance (especially cryptochrome CRY1 at optimal light intensity and phytochrome PHYA at low light). Efficient cold acclimation at optimal light was associated with upregulation of trans-zeatin in leaves and roots, while at low light, cytokinin (except cis-zeatin) content remained diminished. Cold stresses induced elevation of jasmonic acid and salicylic acid (in roots). Low light at optimal conditions resulted in strong suppression of cytokinins, jasmonic and salicylic acid.

• Klíčová slova
• auxin, combined stress, cryptochrome, cytokinin, gene expression, gibberellin, phytochrome, plant hormones,
• MeSH
• aklimatizace * MeSH
• Arabidopsis * genetika   metabolismus   MeSH
• proteiny huseníčku * biosyntéza   genetika   MeSH
• regulace genové exprese u rostlin * MeSH
• světlo * MeSH
• zmrazování * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• proteiny huseníčku * MeSH

To elucidate the effect of light intensity on the cold response (5°C; 7 days) in Arabidopsis thaliana, we compared the following parameters under standard light (150 μmol m-2 s-1), low light (20 μmol m-2 s-1), and dark conditions: membrane damage, photosynthetic parameters, cytokinin oxidase/dehydrogenase (CKX) activity, phytohormone levels, and transcription of selected stress- and hormone-related genes and proteome. The impact of cytokinins (CKs), hormones directly interacting with the light signaling pathway, on cold responses was evaluated using transformants overexpressing CK biosynthetic gene isopentenyl transferase (DEX:IPT) or CK degradation gene HvCKX2 (DEX:CKX) under a dexamethasone-inducible promoter. In wild-type plants, cold treatment under light conditions caused down-regulation of CKs (in shoots) and auxin, while abscisic acid (ABA), jasmonates, and salicylic acid (SA) were up-regulated, especially under low light. Cold treatment in the dark strongly suppressed all phytohormones, except ABA. DEX:IPT plants showed enhanced stress tolerance associated with elevated CK and SA levels in shoots and auxin in apices. Contrarily, DEX:CKX plants had weaker stress tolerance accompanied by lowered levels of CKs and auxins. Nevertheless, cold substantially diminished the impact from the inserted genes. Cold stress in dark minimized differences among the genotypes. Cold treatments in light strongly up-regulated stress marker genes RD29A, especially in roots, and CBF1-3 in shoots. Under control conditions, their levels were higher in DEX:CKX plants, but after 7-day stress, DEX:IPT plants exhibited the highest transcription. Transcription of genes related to CK metabolism and signaling showed a tendency to re-establish, at least partially, CK homeostasis in both transformants. Up-regulation of strigolactone-related genes in apices and leaves indicated their role in suppressing shoot growth. The analysis of leaf proteome revealed over 20,000 peptides, representing 3,800 proteins and 2,212 protein families (data available via ProteomeXchange, identifier PXD020480). Cold stress induced proteins involved in ABA and jasmonate metabolism, antioxidant enzymes, and enzymes of flavonoid and glucosinolate biosynthesis. DEX:IPT plants up-regulated phospholipase D and MAP-kinase 4. Cold stress response at the proteome level was similar in all genotypes under optimal light intensity, differing significantly under low light. The data characterized the decisive effect of light-CK cross-talk in the regulation of cold stress responses.

• Klíčová slova
• acclimation, cold stress, cytokinin, cytokinin oxidase/dehydrogenase, isopentenyl transferase, karrikin, light intensity, phytohormone,
• Publikační typ
• časopisecké články MeSH

BACKGROUND: Although many genetic manipulations of crops providing biofortified or safer food have been done, the acceptance of biotechnology crops still remains limited. We report on a transgenic barley expressing the multi-functional protein osmotin that improves plant defense under stress conditions. METHODS: An Agrobacterium-mediated technique was used to transform immature embryos of the spring barley cultivar Golden Promise. Transgenic barley plants of the T0 and T1 generation were evaluated by molecular methods. Transgenic barley tolerance to stress was determined by chlorophyll, total protein, malondialdehyde and ascorbate peroxidase content. Methanol extracts of i) Fusarium oxysporum infected or ii) salt-stressed plants, were characterized by their acute toxicity effect on human dermal fibroblasts (HDF), genotoxicity and affection of biodiversity interactions, which was tested through monitoring barley natural virus pathogen-host interactions-the BYDV and WDV viruses transmitted to the plants by aphids and leafhoppers. RESULTS: Transgenic plants maintained the same level of chlorophyll and protein, which significantly declined in wild-type barley under the same stressful conditions. Salt stress evoked higher ascorbate peroxidase level and correspondingly less malondialdehyde. Osmotin expressing barley extracts exhibited a lower cytotoxicity effect of statistical significance than that of wild-type plants under both types of stress tested on human dermal fibroblasts. Extract of Fusarium oxysporum infected transgenic barley was not able to damage DNA in the Comet assay, which is in opposite to control plants. Moreover, this particular barley did not affect the local biodiversity. CONCLUSION: Our findings provide a new perspective that could help to evaluate the safety of products from genetically modified crops.

• MeSH
• bezpečnost potravin * MeSH
• biologická adaptace MeSH
• ektopická exprese * MeSH
• fyziologický stres genetika   MeSH
• geneticky modifikované rostliny MeSH
• interakce hostitele a patogenu genetika   MeSH
• ječmen (rod) genetika   MeSH
• lidé MeSH
• rekombinantní proteiny * MeSH
• rostlinné proteiny genetika   MeSH
• tabák genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• rekombinantní proteiny * MeSH
• rostlinné proteiny MeSH

The contents of endogenous brassinosteroids (BRs) together with various aspects of plant morphology, water management, photosynthesis and protection against cell damage were assessed in two maize genotypes that differed in their drought sensitivity. The presence of 28-norbrassinolide in rather high quantities (1-2 pg mg-1 fresh mass) in the leaves of monocot plants is reported for the first time. The intraspecific variability in the presence/content of the individual BRs in drought-stressed plants is also described for the first time. The drought-resistant genotype was characterised by a significantly higher content of total endogenous BRs (particularly typhasterol and 28-norbrassinolide) compared with the drought-sensitive genotype. On the other hand, the drought-sensitive genotype showed higher levels of 28-norcastasterone. Both genotypes also differed in the drought-induced reduction/elevation of the levels of 28-norbrassinolide, 28-norcastasterone, 28-homocastasterone and 28-homodolichosterone. The differences observed between both genotypes in the endogenous BR content are probably correlated with their different degrees of drought sensitivity, which was demonstrated at various levels of plant morphology, physiology and biochemistry.

• MeSH
• brassinosteroidy farmakologie   MeSH
• fotosyntéza * MeSH
• fyziologický stres * MeSH
• genotyp MeSH
• kukuřice setá účinky léků   genetika   růst a vývoj   MeSH
• listy rostlin účinky léků   genetika   růst a vývoj   MeSH
• období sucha * MeSH
• regulátory růstu rostlin farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• brassinosteroidy MeSH
• regulátory růstu rostlin MeSH