BACKGROUND: Autologous vein grafts are widely used for bypass procedures in cardiovascular surgery. However, these grafts are susceptible to failure due to vein graft disease. Our study aimed to evaluate the impact of the latest-generation FRAME external support on vein graft remodeling in a preclinical model. METHODS: We performed autologous internal jugular vein interposition grafting in porcine carotid arteries for one month. Four grafts were supported with a FRAME mesh, while seven unsupported grafts served as controls. The conduits were examined through flowmetry, angiography, macroscopy, and microscopy. RESULTS: The one-month patency rate of FRAME-supported grafts was 100% (4/4), whereas that of unsupported controls was 43% (3/7, Log-rank p = 0.071). On explant angiography, FRAME grafts exhibited significantly more areas with no or mild stenosis (9/12) compared to control grafts (3/21, p = 0.0009). Blood flow at explantation was higher in the FRAME grafts (145 ± 51 mL/min) than in the controls (46 ± 85 mL/min, p = 0.066). Area and thickness of neo-intimal hyperplasia (NIH) at proximal anastomoses were similar for the FRAME and the control groups: 5.79 ± 1.38 versus 6.94 ± 1.10 mm2, respectively (p = 0.558) and 480 ± 95 vs. 587 ± 52 μm2/μm, respectively (p = 0.401). However, in the midgraft portions, the NIH area and thickness were significantly lower in the FRAME group than in the control group: 3.73 ± 0.64 vs. 6.27 ± 0.64 mm2, respectively (p = 0.022) and 258 ± 49 vs. 518 ± 36 μm2/μm, respectively (p = 0.0002). CONCLUSIONS: In our porcine model, the external mesh FRAME improved the patency of vein-to-carotid artery grafts and protected them from stenosis, particularly in the mid regions. The midgraft neo-intimal hyperplasia was two-fold thinner in the meshed grafts than in the controls.
- Publikační typ
- časopisecké články MeSH
Although the cat flea, Ctenocephalides felis, has been identified as the primary vector of Rickettsia felis, additional flea, tick, mite, and louse species have also been associated with this bacterium by molecular means; however, the role of these arthropods in the transmission of R. felis has not been clarified. Here, we succeeded in culture isolation of R. felis from a host-seeking castor bean tick, Ixodes ricinus, the most common tick in Slovakia. The bacterial isolation was performed on XTC-2 cells at 28 °C using the shell-vial technique. An evaluation of the growth properties was performed for both the XTC-2 and Vero cell lines. We observed R. felis in the infected host cells microscopically by Gimenez staining and immunofluorescence assay. The R. felis isolate was purified by gradient ultracentrifugation and visualized by electron microscopy. Fragments of the genes gltA, ompA, ompB, htrA, rpoB, sca4, rffE, and rrs were amplified and compared with the corresponding sequences of the type strain URRWXCal2 and other R. felis culture -isolated strains. We did not detect any nucleotide polymorphisms; however, plasmid pRFδ, characteristic of the standard strain, was absent in our isolate. Herein, we describe the first successful isolation and characterization of a tick-derived R. felis strain "Danube", obtained from an I. ricinus nymph.
- MeSH
- buněčné linie MeSH
- členovci * MeSH
- klíště * mikrobiologie MeSH
- Rickettsia felis * genetika MeSH
- Rickettsia * genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Poly(ADP-ribose) polymerase 1 (PARP1) is implicated in the detection and processing of unligated Okazaki fragments and other DNA replication intermediates, highlighting such structures as potential sources of genome breakage induced by PARP inhibition. Here, we show that PARP1 activity is greatly elevated in chicken and human S phase cells in which FEN1 nuclease is genetically deleted and is highest behind DNA replication forks. PARP inhibitor reduces the integrity of nascent DNA strands in both wild-type chicken and human cells during DNA replication, and does so in FEN1-/- cells to an even greater extent that can be detected as postreplicative single-strand nicks or gaps. Collectively, these data show that PARP inhibitors impede the maturation of nascent DNA strands during DNA replication, and implicate unligated Okazaki fragments and other nascent strand discontinuities in the cytotoxicity of these compounds.
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
1 svazek : ilustrace, tabulky ; 30 cm
The physico-chemical characteristics of an oral environment of patients with dental alloys and with the diagnosis of metal hypersensitivity will be determined by means of modified LTT. Detection of cytokines produced by mononuclear cells (MC) will be studied with proteomic approach using "protein arrays" technology. Corrosion analysis in vivo including measurment of free corrosion potential of dental alloys will be used. Alloys surface analysis by photoelectron spectroscopy will be performed. For in situ detection of metallic deposits transmission electron microscopy and x-ray microanalysis will be used. Metal ions in sulcular fluid will be analysed by mass spectrometry with inductively bound plasma.
Fyzikálně-chemická charakteristika ústního prostředí, provedená neinvazivními metodami, u pacientů s chrupem sanovaným dentálními slitinami a diagnózou hypersensitivity na kovy, provedenou modifikovaným testem proliferace lymfocytů (LTT). Detekce cytokinů, produkovaných mononukleárními buňkami, s použitím proteomového přístupu a využitím "protein array". Stanovení samovolných korozních potenciálů in vivo. Povrchová analýza dentálních slitin pomocí fotoelektronové spektroskopie. Analýza korozí napadenýchtkání metodou transmisní elektronové mikroskopie a x-ray mikroanalýzou. Stanovení kovových iontů v sulkulární tekutině pomocí hmotnostní spektrometrie s induktivně vázaným plazmatem.
- MeSH
- aktivace lymfocytů MeSH
- alergie MeSH
- cytokiny MeSH
- hmotnostní spektrometrie MeSH
- koroze MeSH
- kovy imunologie MeSH
- mikroanalýza elektronovou sondou MeSH
- proliferace buněk MeSH
- transmisní elektronová mikroskopie MeSH
- zubní slitiny škodlivé účinky MeSH
- Konspekt
- Stomatologie
- NLK Obory
- zubní lékařství
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
Naše studie se zabývá kinetikou akutní zánětlivé reakce (interleukinů a vaskulárního endoteliálního růstového faktoru VEGF) po traumatu mozku ve vztahu k aktivaci mikrogliálních mozkových buněk u pacientů s expanzní kontuzí v rámci sekundárního traumatu mozku. Interleukin IL-6, monocyt chemoatrahující protein (MCP-1) a VEGF mají rozdílný časový nástup u skupin pacientů s rozvojem a bez rozvoje expanzní kontuze mozku (p<0.05). Hodnoty VEGF v krvi byly významně vyšší u pacientů s nekomplikovanou mozkovou kontuzí ve srovnání s nižšími hodnotami u pacientů s expanzní kontuzí mozku. Pacienti s rozvojem sepse měli výrazný vzestup koncentrací TNF alpha a interleukinu IL-8 v průběhu prvních 72 hodin. P-selektin glykoprotein ligand PSGL a CD68 imunopozitivní mikrogliové elementy byly detekovány u obou typů, ložiskových i difuzních poranění mozku, zejména v perivaskulárních prostorech a imunopozitivita korelovala s nálezem telolysosomů v cytoplasmě mikroglie. Pozorovali jsme, že některé polymorfismy genů PAI-1, MTHFR, eNOS, VEGF a Apo E při genetickém vyšetření pacientů s traumatem mozku byly spojené s projevem ucpávání kapilár nahromaděnými leukocyty u případů expanzních kontuzí mozku.
Our present study was aimed to investigate time-profile kinetics of interleukins, vascular endothelial growth factor (VEGF) in acute inflammatory response following traumatic brain injury, and the influence of activated microglial cells in patients who developed severe space occupying lesion (SOL) of secondary traumatic brain injury. Interleukins IL-6, monocyte chemo attractant protein (MCP-1), and VEGF had a significant different time-profile kinetics (p<0.05) in patient with, and without expansive traumatic brain contusions (SOL). The serum VEGF was significantly higher in trauma patients with uncomplicated brain contusions, and lower in patients with SOL. The patients with septic complications developed the sudden increase of TNF alpha and IL-8 within the first 72 hours. Our data suggested PSGL and CD68 immunopositivity of microglial cells in both focal and diffuse TBI, predominantly in perivascular space correlated with telolysosome formation in cytoplasma. Polymorphism of PAI-1, MTHFR, eNOS, VEGF, and Apo E genes in TBI were in patients with SOL were bound to show up leucocyte plugging in capillaries.
- MeSH
- chemokin CCL2 biosyntéza fyziologie chemie MeSH
- imunohistochemie MeSH
- interleukin-6 biosyntéza fyziologie chemie MeSH
- interleukin-8 fyziologie MeSH
- lidé MeSH
- polymorfismus genetický MeSH
- poranění mozku * genetika patofyziologie MeSH
- TNF-alfa biosyntéza fyziologie chemie MeSH
- vaskulární endoteliální růstové faktory biosyntéza fyziologie chemie MeSH
- zánět patofyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Úvod: Do diagnostiky kardiomyopatií ve forenzní medicíně patří metody světelné i elektronové mikroskopie a v současné době i metody molekulární biologie. Případ: Vyšetření myokardu u 27-letého muž, který zkolaboval při cestě do práce, přivolané RZS se nepodařilo obnovit vitální funkce. Výsledky: Světelnou a elektronovou mikroskopií byly prokázány změny svědčící pro kardiomyopatii (nepravidelně probíhající hypertrofické kardiomyocyty, ultrastrukturálně neuspořádanost Z proužků, zmnožení mitochondrií, myofibrily probíhající longitudinálně i transverzálně). Genetické vyšetření prokázalo mutaci beta-aktinu. Závěr: Kardiomyopatie může být příčinou náhlého úmrtí u mladých jedinců a její diagnostika vyžaduje interdisciplinární spolupráci.
Introduction: Light microscopy and electron microscopy rank among methods to diagnose of cardiomyopathy in forensic medicine, and, recently, the methods of molecular biology. Methods: Investigation of 27 year old man who collapsed on his way to work. The Rescuers did not succeed resuscitation of vital function. Samples were H-E stained and processed for the electron microscopy. RNA was isolated from the tissue for the alpha, beta, gama actine primer investigation. Results: By H-E staining we proved irregular hypertrophic cardiomyocytes (disarray) with the links and loci patches of thin fibrosis. Ultrastructurally we diagnosed a disarray of Z-bands, accumulation of mitochondria, rectangular nuclei of cardiomyocytes. We have detected rare plasmocytes and leucocytes with specific granules in cytoplasma. In the electronogrames we can see myofibriles oriented longitudinally and transversally. A genetic examination demonstrated beta actin mutation. Conclusion: Cardiomyopathy can be a cause of sudden and unexpected death in young individuals and its diagnostics requires an interdisciplinary collaboration.
- Klíčová slova
- ultrastruktura kardiomyocytu, genové mutace,
- MeSH
- aktiny genetika MeSH
- bodová mutace MeSH
- dospělí MeSH
- elektronová mikroskopie MeSH
- genetické testování MeSH
- hypertrofická kardiomyopatie * patologie MeSH
- kardiomyocyty patologie ultrastruktura MeSH
- lidé MeSH
- myokard patologie ultrastruktura MeSH
- náhlá srdeční smrt * etiologie MeSH
- pitva MeSH
- polymerázová řetězová reakce MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The concept of vena contracta space reduction in tricuspid valve position was tested in an animal model. Feasibility of specific artificial obturator body (REMOT) fixed to the right ventricular apex and interacting with tricuspid valve leaflets was evaluated in three different animal studies. Catheter-based technique was used in three series of experiment in 7 sheep. First acute study was designed for evaluation if the screwing mode of guide wire anchoring to the right ventricular apex is feasible for the whole REMOT body fixing. Longer study was aimed to evaluate stability of the REMOT body in desired position when fixing the screwing wire on its both ends (to the right ventricular apex and to the skin in the neck area). X-ray methods and various morphological methods were used. The third acute study was intended to the REMOT body deployment without any fixing wire. In all of 7 sheep the REMOT was successfully inserted into the right heart cavities and then fixed to the right ventricular apex area. When the REMOT was left in situ more than 6 months it was stable, induced adhesion to the tricuspid valve leaflet and was associated with a specific cell invasion. Releasing of the REMOT from the guiding tools was also successfully verified. Deployment of the obturator body in the aim to reduce the tricuspid valve orifice is feasible and well tolerated in the short and longer term animal model. Specific cell colonization including neovascularization of the obturator body was observed.
- MeSH
- chirurgická náhrada chlopně metody MeSH
- ovce MeSH
- pilotní projekty MeSH
- studie proveditelnosti MeSH
- trikuspidální chlopeň patologie chirurgie MeSH
- trikuspidální insuficience patologie chirurgie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
