There is increasing evidence indicating a role for Fusobacterium nucleatum (F. nucleatum) in colorectal cancer (CRC) development and prognosis. This study evaluated F. nucleatum as a prognostic biomarker, by assessing its association with post-diagnosis survival from CRC. From September 2008 to April 2012 CRC patients (n = 190) were recruited from three hospitals within the Czech Republic. F. nucleatum DNA copies were measured in adjacent non-malignant and colorectal tumor tissues using quantitative real-time PCR. Cox Proportional Hazards (HR) models were applied to evaluate the association between F. nucleatum DNA and overall survival, adjusting for key confounders. Risk prediction modeling was conducted to evaluate the ability to predict survival based on F. nucleatum status. High, compared with low, levels of F. nucleatum in colorectal tumor tissues were associated with poorer overall survival (adjusted HR 1.68, 95% CI 1.02-2.77), which was slightly attenuated after additional adjustment for microsatellite instability status. However, inclusion of F. nucleatum in risk prediction models did not improve the ability to identify patients who died beyond known prognostic factors such as disease pathology staging. Although the increased presence of F. nucleatum was associated with poorer prognosis in CRC patients, this may have limited clinical relevance as a prognostic biomarker.
- MeSH
- analýza přežití MeSH
- biologické markery analýza MeSH
- DNA bakterií analýza MeSH
- Fusobacterium nucleatum genetika MeSH
- hodnocení rizik MeSH
- infekce bakteriemi rodu Fusobacterium mikrobiologie MeSH
- kohortové studie MeSH
- kolorektální nádory mortalita patologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- prognóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
The colonization of the oral cavity by cariogenic and periodontal pathogens occurs earlier than previously thought. This study aimed to identify the presence and quantity of representative cariogenic and periodontal pathogens in the oral cavities of 6- and 12-month olds and to evaluate the influence of C-section delivery on early Streptococcus mutans (Sm) colonization of the oral cavity. The research cohort was composed of 59 infants (35 infants were delivered vaginally and 24 via C-section) and their mothers. At 6 months of age, the infants were examined, and unstimulated saliva samples were collected. Variables concerning mothers were DMF index and salivary levels of Sm. Repeated saliva samples were taken 6 months later. The representative cariogenic and periodontal microorganisms were identified, and their quantities were measured using a polymerase chain reaction-based method. The relationships between the presence of detected microbes, the mode of delivery, and maternal variables were evaluated using paired t tests, chi-squared test, and ANOVAs. High rates of cariogenic bacteria, Aggregatibacter actinomycetemcomitans (Aa) and Fusobacterium nucleatum (Fn), were found in both infant cohorts. An analysis of the differences between delivery methods revealed that the group of 6-month-old vaginally delivered infants had a significantly higher amount of Sm. We conclude that the cariogenic bacteria, Aa and Fn, are present in edentulous infants. This presence increases in the months following the eruption of the deciduous teeth. Results did not confirm the influence of C-section delivery on the early Sm colonization of the oral cavity.
- MeSH
- Aggregatibacter actinomycetemcomitans izolace a purifikace MeSH
- bakteriální nálož MeSH
- Fusobacterium nucleatum izolace a purifikace MeSH
- kojenec MeSH
- lidé MeSH
- mikrobiota * MeSH
- polymerázová řetězová reakce MeSH
- rizikové faktory MeSH
- sliny mikrobiologie MeSH
- Streptococcus mutans izolace a purifikace MeSH
- studie případů a kontrol MeSH
- ústa mikrobiologie MeSH
- věkové faktory MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Conventional chemical antiseptics used for treatment of oral infections often produce side-effects, which restrict their long-term use. Plants are considered as perspective sources of novel natural antiseptics. However, little is still known about their inhibitory properties against oral pathogens. The objective of this study was to test in vitro antimicrobial activities of generally recognized as safe (GRAS) species against planktonic cultures of cariogenic, periodontal and candidal microorganisms and identify active compounds of the most active extracts. Growth-inhibitory effects of ethanol extracts from 109 GRAS plant species, six Humulus lupulus cultivars and two hop supercritical CO2 extracts were evaluated using broth microdilution method. The chemical analysis was done through high-performance liquid chromatography. Best results were obtained for supercritical CO2 and ethanol extracts of H. lupulus with minimum inhibitory concentrations (MIC) ≥8 μg/mL and ≥16 μg/mL, respectively. The chemical analysis of supercritical CO2H. lupulus extracts revealed that α- and β-acids were their main constituents. Capsicum annuum and Capsicum frutescens showed antibacterial effect against Streptococcus sobrinus and Streptococcus salivarius (MIC=64-128 μg/mL). These strains were further inhibited by Zanthoxylum clava-herculis (MIC=64-128 μg/mL) and Myristica fragrans (both MIC≥128 μg/mL). The latter also exhibited antimicrobial activity against Fusobacterium nucleatum (MIC=64 μg/mL). Punica granatum possessed inhibitory effects against Candida albicans (MIC=128 μg/mL) and F. nucleatum (MIC=64 μg/mL). The results indicate that supercritical CO2H. lupulus extracts together with ethanol extracts of C. annuum, C. frutescens, M. fragrans, P. granatum and Z. clava-herculis are promising materials for further investigation on new antiseptic agents of oral care products.
- MeSH
- antibakteriální látky chemie MeSH
- Candida albicans účinky léků MeSH
- Capsicum chemie MeSH
- ethanol MeSH
- Fusobacterium nucleatum účinky léků MeSH
- Humulus chemie MeSH
- mikrobiální testy citlivosti MeSH
- Myristica chemie MeSH
- rostlinné extrakty chemie MeSH
- Streptococcus sobrinus účinky léků MeSH
- Zanthoxylum chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Commensal bacteria in the colon may play a role in colorectal cancer (CRC) development. Recent studies from North America showed that Fusobacterium nucleatum (Fn) infection is over-represented in disease tissue versus matched normal tissue in CRC patients. Using quantitative real-time polymerase chain reaction (qPCR) of DNA extracted from colorectal tissue biopsies and surgical resections of three European cohorts totalling 122 CRC patients, we found an over-abundance of Fn in cancerous compared to matched normal tissue (p<0.0001). To determine whether Fn infection is an early event in CRC development, we assayed Fn in colorectal adenoma (CRA) tissue from 52 Irish patients. While for all CRAs the Fn level was not statistically significantly higher in disease versus normal tissue (p=0.06), it was significantly higher for high-grade dysplasia (p=0.015). As a secondary objective, we determined that CRC patients with low Fn levels had a significantly longer overall survival time than patients with moderate and high levels of the bacterium (p=0.008). The investigation of Fn as a potential non-invasive biomarker for CRC screening showed that, while Fn was more abundant in stool samples from CRC patients compared to adenomas or controls, the levels in stool did not correlate with cancer or adenoma tissue levels from the same individuals. This is the first study examining Fn in the colonic tissue and stool of European CRC and CRA patients, and suggests Fn as a novel risk factor for disease progression from adenoma to cancer, possibly affecting patient survival outcomes. Our results highlight the potential of Fn detection as a diagnostic and prognostic determinant in CRC patients.
- MeSH
- adenom * MeSH
- bakteriální nálož MeSH
- feces mikrobiologie MeSH
- Fusobacterium nucleatum genetika izolace a purifikace MeSH
- Kaplanův-Meierův odhad MeSH
- kohortové studie MeSH
- kolorektální nádory MeSH
- lidé středního věku MeSH
- lidé MeSH
- missense mutace MeSH
- molekulární typizace MeSH
- nádorový supresorový protein p53 genetika MeSH
- prognóza MeSH
- protoonkogenní proteiny B-raf genetika MeSH
- protoonkogenní proteiny p21(ras) MeSH
- protoonkogenní proteiny genetika MeSH
- ras proteiny genetika MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- staging nádorů MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
BACKGROUND: Recently, the dental literature has focused mainly on the microbial colonization of healthy full-term infants and their mothers or caretakers. However, oral microbial acquisition by premature infants has not been adequately investigated, and the correlation between pre-term birth and the presence of cariogenic and periodontal pathogens has not been determined. The aim of this study was to identify the presence and quantities of representative cariogenic and periodontal pathogens in the oral cavities of 12-month-old infants and compare the occurrence of these microbes between a cohort of pre-term infants with very low birthweights and a control cohort comprising full-term infants. METHODS: The research cohort was composed of 69 one-year-old infants, of whom 24 were born prematurely with very low birthweights and 45 of whom were carried to full term. Information regarding the infants' gestational age, mode of delivery, general health status, birthweight and antibiotic use were obtained from hospital records and through oral interviews. At 12 months of age, both groups of infants were examined, and unstimulated saliva samples from the dorsum of the tongue and dental plaque samples were collected. The microorganisms (Streptococcus mutans, Lactobacillus spp., Actinomyces spp., Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Peptostreptococcus micros, Prevotella intermedia, Fusobacterium nucleatum) were identified and their quantities were evaluated using a PCR-based method. The chi-squared and Fisher's factorial tests were used for the statistical evaluations. RESULTS: The infants had a high prevalence of cariogenic microbes and of Fusosbacterium nucleatum and Aggregatibacter actinomycetemcomitans. Cariogenic microbes were detected in 91.7% of the very low birthweight infants and in all full-term infants. Periodontal pathogens were present in 83% of the pre-term infants and in 96% of the full-term infants. A significant difference was found between the cohorts in terms of the presence of S. mutans. Most of the very low birthweight infants had negative values of this microbe, while the full-term infants had positive values. CONCLUSIONS: This study confirms the early transmission of representative cariogenic and periodontal pathogens to the oral cavity of one-year-old infants and a higher prevalence of S. mutans in full-term infants than in premature infants.
- MeSH
- Actinomyces izolace a purifikace MeSH
- Aggregatibacter actinomycetemcomitans izolace a purifikace MeSH
- antibakteriální látky terapeutické užití MeSH
- bakteriální nálož MeSH
- Fusobacterium nucleatum izolace a purifikace MeSH
- gestační stáří MeSH
- jazyk mikrobiologie MeSH
- kohortové studie MeSH
- kojenec MeSH
- Lactobacillus izolace a purifikace MeSH
- lidé MeSH
- nemoci parodontu mikrobiologie MeSH
- novorozenec nedonošený * MeSH
- novorozenec s nízkou porodní hmotností * MeSH
- novorozenec MeSH
- porod v termínu MeSH
- porodní hmotnost MeSH
- sliny mikrobiologie MeSH
- Streptococcus mutans izolace a purifikace MeSH
- studie případů a kontrol MeSH
- ústa mikrobiologie MeSH
- vedení porodu MeSH
- zdravotní stav MeSH
- zubní kaz mikrobiologie MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- MeSH
- čištění zubů MeSH
- dítě MeSH
- Fusobacterium nucleatum patogenita MeSH
- komplikace těhotenství krev MeSH
- lidé MeSH
- orální hygiena * MeSH
- parodontitida diagnóza etiologie prevence a kontrola terapie MeSH
- zubní hygienici MeSH
- zubní kámen patologie prevence a kontrola MeSH
- zubní kaz patologie prevence a kontrola MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- ženské pohlaví MeSH
Patients with fixed orthodontic appliances tend to exhibit signs of gingivitis and gingival enlargement with false pockets. The aim of this study was to evaluate the occurrence of periodontal pathogens in patients with clinical manifestation of plaque-associated gingivitis treated with fixed orthodontic appliances. Thirty-two consecutive orthodontic patients – 11 females (age range from 10 to 32; mean age 17.43 years) and 21 males (age range from 12 to 28; average age 16.25 years) were included in this study. All individuals were diagnosed with clinical signs of gingivitis. CPI and PBI were evaluated. Subgingival plaque samples were collected after periodontal examination. Genomic DNA was extracted from these samples and bacterial detection was performed by polymerase chain reaction (Test VariOr-Dento). We recorded the following occurrence of periodontal pathogens: Actinobacillus actinomycetemcomitans – 25 %, Porphyromonas gingivalis – 31.25 %, Treponema denticola – 43.75 %, Tannerella forsythensis – 59 %, Prevotella intermedia – 65.6 %, Peptostreptococcus micros – 75 %, and Fusobacterium nucleatum – 100 %. Two and more pathogens were detected simultaneously in 93.75 % of the patients. Simultaneous occurrence of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis was recorded in 9.37 % of the patients. Likewise, simultaneous occurrence of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythensis was registered in 15.6 % of the patients. The number of Porphyromonas gingivalis bacteria in the sample correlated significantly (p=0.01) with the values of the CPI index (evaluated by the Kruskal-Wallis ANOVA test). In this study 80 % of the patients treated with the fixed orthodontic appliance and with clinical manifestations of plaqueinduced gingivitis are bearers of periodontal pathogens in a quantity significant for the damage of periodontium. To treat these patients successfully, collaboration with a periodontologist is highly recommended.
- MeSH
- Aggregatibacter actinomycetemcomitans izolace a purifikace MeSH
- bakteriální infekce diagnóza etiologie komplikace MeSH
- DNA bakterií izolace a purifikace MeSH
- financování organizované MeSH
- Fusobacterium nucleatum izolace a purifikace MeSH
- gingivitida diagnóza etiologie terapie MeSH
- lidé MeSH
- ortodontické aparáty škodlivé účinky MeSH
- Peptostreptococcus izolace a purifikace MeSH
- polymerázová řetězová reakce metody využití MeSH
- Porphyromonas gingivalis izolace a purifikace MeSH
- Prevotella intermedia izolace a purifikace MeSH
- prospektivní studie MeSH
- statistika jako téma metody MeSH
- Treponema denticola izolace a purifikace MeSH
- zubní plak diagnóza etiologie komplikace MeSH
- Check Tag
- lidé MeSH
Infekce vyvolaná anaerobní gramnegativní tyčkou Fusobacterium nucleatum patří mezi endogenní infekce. U jednačtyřicetiletého muže se vytvořily mnohočetné mozkové abscesy odontogenního původu. Příčinou byla velmi špatná hygiena dutiny ústní a absence návštěv u stomatologa. Ve hnisu získaného stereoaspirací jednoho mozkového ložiska byla prokázána deoxyribonukleová kyselina bakterie Fusobacterium nucleatum. Byla provedena extrakce několika zubů s periapikálními ložisky. Pacient byl nejprve léčen empiricky kombinací cefotaximu a metronidazolu (18 dnů), následně byl dlouhodobě (8 měsíců) podáván cotrimoxazol a rifampicin. Při vyšetření magnetickou rezonancí za 4 a 8 měsíců od počátku onemocnění přetrvávaly jen reziduální změny v mozkovém parenchymu, pacient byl zcela bez obtíží, bez neurologického nálezu a chodil do práce.
Infection caused by the anaerobic gram-negative rod Fusobacterium nucleatum belongs to endogenous infections. A 41-year-old man developed multiple brain abscesses of odontogenic origin. The main causes were poor oral hygiene and no dental check-ups. Pus aspired from a site in the brain contained DNA of the bacterium Fusobacterium nucleatum. Several teeth with periapical abscesses were extracted. Initially, the patient was treated empirically with a combination of cefotaxime and metronidazole (18 days). Subsequently, co-trimoxazole and rifampicin were administered (8 months). Four and eight months after the onset of the disease, MRI scans revealed only persistent residual changes in the brain parenchyma; the patient was asymptomatic, with no neurological signs and back at work.
- MeSH
- absces mozku diagnóza etiologie mikrobiologie MeSH
- dospělí MeSH
- fokální infekce zubní komplikace mikrobiologie MeSH
- Fusobacterium nucleatum MeSH
- infekce bakteriemi rodu Fusobacterium diagnóza etiologie MeSH
- lidé MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
