In yeast, the STB5 gene encodes a transcriptional factor belonging to binuclear cluster class (Zn2Cys6) of transcriptional regulators specific to ascomycetes. In this study, we prepared the Kluyveromyces lactis stb5Δ strain and assessed its responses to different stresses. We showed that KlSTB5 gene is able to complement the deficiencies of Saccharomyces cerevisiae stb5Δ mutant. The results of phenotypic analysis suggested that KlSTB5 gene deletion did not sensitize K. lactis cells to oxidative stress inducing compounds but led to Klstb5Δ resistance to 4-nitroquinoline-N-oxide and hygromycin B. Expression analysis indicated that the loss of KlSTB5 gene function induced the transcription of drug efflux pump encoding genes that might contribute to increased 4-nitroquinoline-N-oxide and hygromycin B tolerance. Our results show that KlStb5p functions as negative regulator of some ABC transporter genes in K. lactis.
- MeSH
- 4-Nitroquinoline-1-oxide pharmacology MeSH
- Gene Deletion MeSH
- Fungal Proteins genetics metabolism MeSH
- Kluyveromyces drug effects genetics metabolism MeSH
- Oxidative Stress drug effects MeSH
- Gene Expression Regulation, Fungal drug effects MeSH
- Saccharomyces cerevisiae Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae drug effects genetics metabolism MeSH
- Transcription Factors genetics metabolism MeSH
- Publication type
- Journal Article MeSH
Plant-derived smoke and certain smoke compounds improve seed germination and enhance seedling growth of many species. Thus, smoke-infused water and the active smoke-derived compounds have the potential to be used in different agricultural and horticultural applications. However, despite these interesting and potentially practical properties, it should also be ascertained whether such compounds may pose a health risk, particularly if they are to be used in the production of food or fodder crops. Amongst some of the aspects that would be important to understand are any possible genotoxic properties that the compounds may possess due to potential carry-over effects. Here, we report on a genotoxicity study of 3,4,5-trimethylfuran-2(5H)-one, a compound from plant-derived smoke previously shown to have germination inhibitory activity. Using two in vitro tests, namely the bacterial VITOTOX® test (with/without S9 metabolic activation) and the cytome assay on human C3A cells, no genotoxicity or toxicity was found. Furthermore, these results support a previous study where a related smoke-derived compound with germination promoting properties was investigated.
- MeSH
- 4-Nitroquinoline-1-oxide toxicity MeSH
- Benzopyrenes toxicity MeSH
- Quinolones toxicity MeSH
- Furans pharmacology MeSH
- Hepatocytes cytology drug effects metabolism MeSH
- Germination drug effects MeSH
- Humans MeSH
- Luciferases genetics metabolism MeSH
- Mutagens pharmacology MeSH
- Cell Line, Tumor MeSH
- Genes, Reporter MeSH
- Plants drug effects MeSH
- Salmonella typhimurium drug effects genetics growth & development MeSH
- Seeds drug effects growth & development MeSH
- SOS Response, Genetics drug effects MeSH
- Mutagenicity Tests MeSH
- Transcription Factors genetics metabolism MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Several authors have reported schistosomiasis caused by Schistosoma haematobium in the female genital tract of patients in endemic areas. This work describes tubal schistosomiasis by Schistosoma mansoni in a Brazilian woman submitted to hysterectomy for uterine myomatosis and metrorrhagia. Macroscopy evidenced hydrosalpinx of the left tube and multiple Schistosoma mansoni eggs were identified by anatomopathological examination. This article illustrates a rare form of schistosomiasis as the cause of tubal damage.
- MeSH
- Adult MeSH
- Drug Therapy methods utilization MeSH
- Hysterectomy methods utilization MeSH
- Humans MeSH
- Metrorrhagia surgery MeSH
- Microscopy methods utilization MeSH
- Myoma surgery MeSH
- Fallopian Tube Diseases diagnosis etiology parasitology MeSH
- Oxamniquine administration & dosage therapeutic use MeSH
- Praziquantel administration & dosage therapeutic use MeSH
- Schistosomiasis mansoni diagnosis etiology parasitology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
- Geographicals
- Brazil MeSH
Differential pulse voltammetry, direct current voltammetry, adsorptive stripping voltammetry and HPLC with electrochemical detection were used for the determination of 5-amino-6-nitroquinoline at a carbon paste electrode. The methods are based either on anodic oxidation or cathodic reduction of this substance, whose electrochemical behavior at carbon paste electrode was further studied by cyclic voltammetry. Practical applicability of these methods was demonstrated on the determination of 5-amino-6-nitroquinoline in model samples of drinking and river water. The detection limit was 2.0 × 10–6 mol l–1 for anodic differential pulse voltammetry in a mixture of Britton–Robinson buffer (pH 11)–methanol 1:1 (v/v) and 1.6 × 10–7 mol l–1 for HPLC with electrochemical detection (E = +1.2 V) in a mobile phase Britton–Robinson buffer (pH 7)–methanol 1:9 (v/v).
- MeSH
- Hepatomegaly diagnosis etiology parasitology MeSH
- Snails parasitology growth & development MeSH
- Oxamniquine administration & dosage adverse effects therapeutic use MeSH
- Intestinal Diseases, Parasitic etiology complications transmission MeSH
- Liver Diseases, Parasitic etiology complications transmission MeSH
- Praziquantel administration & dosage adverse effects therapeutic use MeSH
- Developing Countries MeSH
- Schistosomiasis diagnosis etiology therapy MeSH
- Splenomegaly diagnosis etiology parasitology MeSH
- Hepatitis Viruses pathogenicity growth & development MeSH
Genotoxická aktivita dvojjadrových [Cu2(RCOO)4(N-oxide)2] komplexov (R = 2-chlórfenoxymetyl) bola testovaná voči kmeňom Salmonella typhimurium (TA97, TA100 a TA102) Amesovou metódou in vitro a porovnávaná s aktivitou volných N-oxidov - 4-nitrochinolín-N-oxidom, 4-nitropyridín-N-oxidom a 2-metyl-4-nitropyridín-N-oxidom. Komplexáciou 4-nitrochinolín-N-oxidu s bis(2-chlórfenoxyacetáto)meďnatým fragmentom bol redukovaný jeho mutagénny účinok (9-krát, vzťahované na obsah N-oxidu). V prípade 4-nitropyridín-N-oxidu, 2-metyl-4-mtropyridín-N-oxidu a ich bis(N-oxid)-tetrakis (2-chlórfenoxyacetáto)mednatých komplexov nebol tento fenomén pozorovaný.
Using Ames method in vitro, the genotoxic activity of binuclear [Cu2(RCOO)4(N-oxide)2] complexes (R = 2-chlorophenoxymethyl) was tested against the strains of Salmonella typhimurium (TA97, TA100 and TA102) and compared to that of the free N-oxides - 4-nitroquinoline N-oxide, 4-nitropyridine N-oxide and 2-methyl-4-mtiopyridine N-oxide. Complexation of 4-nitroquinohne N-oxide with bisC2-chlorophenoxyacetato)copper(II) fragment reduced its mutagenic effect (9-times, correlated with N-oxide content). In the case of 4-nitropyridine N-oxide, 2-methyl-4-nitropyridine N-oxide and their bis(N-oxide)tetrakis(2-chlorophenoxyacetato)copper(II) complexes this phenomenon was not observed.
- MeSH
- 4-Nitroquinoline-1-oxide administration & dosage MeSH
- Chlorophenols analogs & derivatives administration & dosage MeSH
- Copper analogs & derivatives administration & dosage MeSH
- Organometallic Compounds administration & dosage MeSH
- Salmonella typhimurium MeSH
- In Vitro Techniques MeSH
- Mutagenicity Tests MeSH
- Toxicity Tests MeSH
