Ochrana člověka za mimořádných událostí je povinný vzdělávací obsah volně navazující na bývalou brannou výchovu, o jejímž obnovení se v kontextu událostí poslední doby opět diskutuje. Nabízí velmi širokou paletu témat, která lze zařadit nejen do výuky chemie, ale i jiných (nejen) přírodních věd. Velmi vhodné je např. zařazení do výuky jako (krátko či střednědobý) projekt zaměřený na téma radioaktivita a jaderné zbraně, které je pro žáky zajímavé. Pro realizaci projektu byla autory článku vytvořena pojmová mapa a návodné otázky, a také seznam doporučených zdrojů informací, které mohou učitelé a jejich žáci využít. Cílem projektu je nejen nabýt nové znalosti a vědomosti v souvislosti s tématem jaderných zbraní a radioaktivity, ale také získat ucelený přehled díky mezipředmětovému uspořádání.
Human protection in emergencies is a compulsory educational content in the Czech Republic loosely related to the former military education. It offers a very wide range of topics that can be included not only in chemistry education, but also in other (not only) natural sciences. For example, it is very suitable to include it as a (short- or medium-term) project focused on radioactivity and nuclear weapons, which is of interest to the students. For this purpose, we have created a concept map and guiding questions, as well as a list of recommended sources of information that teachers and their pupils can use. The aim of the project is not only to gain new knowledge and understanding, but also to gain holistic insight into the topic through cross-curricular educational approach.
- MeSH
- Radiation, Ionizing MeSH
- Nuclear Weapons MeSH
- Humans MeSH
- Radioactivity MeSH
- Education methods MeSH
- Teaching * MeSH
- Check Tag
- Humans MeSH
PURPOSE: Despite advancements in radiation techniques, concerns persist regarding the adverse effects of radiation therapy, particularly cardiotoxicity or radiation-induced heart disease. Recently, arrhythmogenic toxicity has come to the forefront-the impact of radiation therapy on the cardiac conduction system. Our objective was to conduct a dosimetric study and subsequently investigate the feasibility of optimizing the sinoatrial (SA) and atrioventricular (AV) nodes as organs at risk (OARs) in proton radiation therapy for non-small cell lung cancer with N3 disease. PATIENTS AND METHODS: Thirty-two non-small cell lung cancer patients with N3 disease undergoing proton radiation therapy were included. Sinoatrial and AV nodes, along with standard OARs, were delineated. Dosimetric analysis and optimization were performed using intensity-modulated proton therapy. RESULTS: Patients surpassing a predefined SA node dose threshold underwent dose optimization. Proton radiation therapy with pencil beam scanning demonstrated a significant reduction in SA and AV node doses without compromising target volume coverage or significant shift in the dose to other monitored OARs. CONCLUSION: Dose reduction to the SA and AV nodes for pencil beam scanning is a relatively simple task, and the reduction can be very substantial. Larger cohort studies and diverse radiotherapeutic modalities are needed for further validation and refinement of dose constraints.
- Publication type
- Journal Article MeSH
Francisella is a highly infectious gram-negative bacterium that causes tularemia in humans and animals. It can survive and multiply in a variety of cells, including macrophages, dendritic cells, amoebae, and arthropod-derived cells. However, the intracellular life cycle of a bacterium varies depending on the cell type. Shortly after the infection of mammalian cells, the bacterium escapes the phagosome into the cytosol, where it replicates. In contrast, in the amoebae Acanthamoeba castellanii and Hartmannella vermiformis, the bacterium replicates within the membrane-bound vacuole. In recent years, the amoeba Dictyostelium discoideum has emerged as a powerful model to study the intracellular cycle and virulence of many pathogenic bacteria. In this study, we used D. discoideum as a model for the infection and isolation of Francisella novicida-containing vacuoles (FCVs) formed after bacteria invade the amoeba. Our results showed that F. novicida localized in a vacuole after invading D. discoideum. Here, we developed a method to isolate FCV and determined its composition by proteomic analyses. Proteomic analyses revealed 689 proteins, including 13 small GTPases of the Rab family. This is the first evidence of F. novicida-containing vacuoles within amoeba, and this approach will contribute to our understanding of host-pathogen interactions and the process of pathogen vacuole formation, as vacuoles containing bacteria represent direct contact between pathogens and their hosts. Furthermore, this method can be translocated on other amoeba models.
- Publication type
- Journal Article MeSH
CONTEXT: Surgery is the gold standard for the local treatment of primary renal cell carcinoma (RCC), but alternatives are emerging. We conducted a systematic review and meta-analysis to assess the results of prospective studies using definitive stereotactic body radiotherapy (SBRT) to treat primary localised RCC. EVIDENCE ACQUISITION: This review was prospectively registered in PROSPERO (CRD42023447274). We searched PubMed, Embase, Scopus, and Google Scholar for reports of prospective studies published since 2003, describing the outcomes of SBRT for localised RCC. Meta-analyses were performed for local control (LC), overall survival (OS), and rates of adverse events (AEs) using generalised linear mixed models (GLMMs). Outcomes were presented as rates with corresponding 95% confidence intervals (95% CIs). Risk-of-bias was assessed using the ROBINS-I tool. EVIDENCE SYNTHESIS: Of the 2983 records, 13 prospective studies (n = 308) were included in the meta-analysis. The median diameter of the irradiated tumours ranged between 1.9 and 5.5 cm in individual studies. Grade ≥ 3 AEs were reported in 15 patients, and their estimated rate was 0.03 (95%CI: 0.01-0.11; n = 291). One- and two-year LC rates were 0.98 (95%CI: 0.95-0.99; n = 293) and 0.97 (95%CI: 0.93-0.99; n = 253), while one- and two-year OS rates were 0.95 (95%CI: 0.88-0.98; n = 294) and 0.86 (95%CI: 0.77-0.91; n = 224). There was no statistically significant heterogeneity, and the estimations were consistent after excluding studies at a high risk of bias in a sensitivity analysis. Major limitations include a relatively short follow-up, inhomogeneous reporting of renal function deterioration, and a lack of prospective comparative evidence. CONCLUSIONS: The short-term results suggest that SBRT is a valuable treatment method for selected inoperable patients (or those who refuse surgery) with localised RCC associated with low rates of high-grade AEs and excellent LC. However, until the long-term data from randomised controlled trials are available, surgical management remains a standard of care in operable patients.
- Publication type
- Journal Article MeSH
- Review MeSH
INTRODUCTION: Prenatal mental health problems are associated with morbidity for the pregnant person, and their infants are at long-term risk for poor health outcomes. We aim to explore how the SARS-CoV-2 pandemic affected the mental health of pregnant people in the United Kingdom (UK), and to further identify resilience factors which may have contributed to varying mental health outcomes. We also aim to examine the quality of antenatal care provided during the pandemic in the UK and to identify potential inadequacies to enhance preparedness for future events. METHODS: During June-November 2020, we recruited 3666 individuals in the UK for the EPPOCH pregnancy cohort (Maternal mental health during the COVID-19 pandemic: Effect of the Pandemic on Pregnancy Outcomes and Childhood Health). Participants were assessed for depression, anxiety, anger and pregnancy-related anxiety using validated scales. Additionally, physical activity, social support, individualized support and personal coping ability of the respondents were assessed as potential resilience factors. RESULTS: Participants reported high levels of depression (57.05%), anxiety (58.04%) and anger (58.05%). Higher levels of social and individualized support and personal coping ability were associated with lower mental health challenges. Additionally, pregnant individuals in the UK experienced higher depression during the pandemic than that reported in Canada. Finally, qualitative analysis revealed that restrictions for partners and support persons during medical appointments as well as poor public health communication led to increased mental health adversities and hindered ability to make medical decisions. DISCUSSION: This study revealed increased mental health challenges among pregnant individuals in the UK during the SARS-CoV-2 pandemic. These results highlight the need for reassessing the mental health support measures available to pregnant people in the UK, both during times of crisis and in general.
- Publication type
- Journal Article MeSH
Vydání první 381 stran ; 21 cm
Román odehrávající se na počátku 20. století v zapadlém městečku Svatý Jiří. Obyvatelé tohoto zvláštního místa žijí zcela odříznuti od velkých technických zázraků a ruchu velkoměst. Přesto začínají tušit, že se doba mění.; Přejít po vodě suchou nohou, lámat kámen hvízdáním, rozmlouvat s anděly a se sochami svatých – to všechno jsou pro obyvatele zapadlého městečka Svatý Jiří naprosto běžné věci. Na začátku 20. století žijí úplně odtrženě od světa, ale začínají pomalu tušit, že se přiblížila doba velkých technických zázraků, které nahradí ty jejich tradiční. Do městečka se vrací místní rodák Odysseus, považovaný dosud za mrtvého. Jako zkušený světoběžník pomáhá sousedům připravit se na nastávající převratné změny. Současně soupeří s mladším bratrem Tomášem o přízeň krásné a dočasně němé Julie, kterou Tomáš našel před pár lety polomrtvou v lese. Nebývalé vzrušení přinese také správní kontrola z hlavního města, před níž je třeba některé věci, včetně dosud fungujících zázraků, utajit. Všichni se v roce 1904 těší na skvělé nové století, v němž bude všechno krásné a šťastné i bez kouzel. Vše tak, jak to má být. Román s nápaditě využitými prvky magického realismu odkazuje na velká vyprávění minulosti od Odyssea přes Dona Quijota po Tristrama Shandyho.
- Conspectus
- Slovenská literatura
Endophytic microbes are plant-associated microorganisms that reside in the interior tissue of plants without causing damage to the host plant. Endophytic microbes can boost the availability of nutrient for plant by using a variety of mechanisms such as fixing nitrogen, solubilizing phosphorus, potassium, and zinc, and producing siderophores, ammonia, hydrogen cyanide, and phytohormones that help plant for growth and protection against various abiotic and biotic stresses. The microbial endophytes have attained the mechanism of producing various hydrolytic enzymes such as cellulase, pectinase, xylanase, amylase, gelatinase, and bioactive compounds for plant growth promotion and protection. The efficient plant growth promoting endophytic microbes could be used as an alternative of chemical fertilizers for agro-environmental sustainability. Endophytic microbes belong to different phyla including Euryarchaeota, Ascomycota, Basidiomycota, Mucoromycota, Firmicutes, Proteobacteria, and Actinobacteria. The most pre-dominant group of bacteria belongs to Proteobacteria including α-, β-, γ-, and δ-Proteobacteria. The least diversity of the endophytic microbes have been revealed from Bacteroidetes, Deinococcus-Thermus, and Acidobacteria. Among reported genera, Achromobacter, Burkholderia, Bacillus, Enterobacter, Herbaspirillum, Pseudomonas, Pantoea, Rhizobium, and Streptomyces were dominant in most host plants. The present review deals with plant endophytic diversity, mechanisms of plant growth promotion, protection, and their role for agro-environmental sustainability. In the future, application of endophytic microbes have potential role in enhancement of crop productivity and maintaining the soil health in sustainable manner.
- MeSH
- Ascomycota * MeSH
- Bacillus * MeSH
- Bacteria genetics MeSH
- Basidiomycota * MeSH
- Endophytes MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
UNLABELLED: Aim of our study is to analyse the sensitisation profile to molecular components of latex and of food allergens with the use of ALEX2 Allergy Xplorer test and to compare these results with the anamnestical data after latex exposure and with the anamnestical data after exposure to food allergens in atopic dermatitis patients. METHODS: 100 patients were included in the study (49 men and 51 women with the average age 40.6 years). The specific IgE was examined with the use of ALEX2 Allergy Xplorer test. A detailed personal history of allergic reaction to latex and allergic reaction to food allergens was taken in all included patients. RESULTS: The sensitisation to latex was recorded in 17 % of patients, majority of patients have positive results of specific IgE to Hev b 8 without clinical reaction to latex. In 7 % of patients with positive results of specific IgE to Hev b 1, Hev b 3, Hev b 5, Hev b 6.02 and Hev b 11 the contact urticaria or contact dermatitis were recorded. The latex fruit syndrome was recorded in 7 % of patients; in another 10 % of patients we recorded no clinical reaction to latex, but the positive results to molecular components of latex and the clinical symptoms after ingestion of different kinds of fruits. CONCLUSION: The significant relation between the results of specific IgE to molecular components Hev b 3, Hev b 5 and Hev b 6.02 and the clinical reaction to latex was confirmed; these components significantly imply clinical reactivity to latex.
- MeSH
- Allergens * immunology MeSH
- Latex Hypersensitivity * immunology MeSH
- Dermatitis, Atopic * immunology MeSH
- Adult MeSH
- Immunoglobulin E * immunology MeSH
- Latex immunology MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Food Hypersensitivity * immunology MeSH
- Aged MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
