Periodontitis is a chron. inflammatory disease of periodontal tissues initiated by dental plaque G- anaerobic bacteria with ~3-times higher risk in diabetics compared to non-diabetics, most likely due to a different immune reactivity to periodontal bacterial colonisation in hyperglycemia. Proposed project targets so far unsystematically mapped prevalence, type and severity of periodontal complications in diabetics in Czech population and its relationship with diabetes compensation. We will analyse periodontal status (clinical indexes + X-ray), microbial colonisation (DNA diagnostic kit for 7 common periopathogens), potential genetic risk factors (30–45 variants) and concentration of 7 soluble markers of inflammation and bone resorption (ELISA). Study will consist of case-control analysis (i.e. non- vs. diabetics, ~400 subjects) and prospective follow-up of diabetic cohort with the aim to predict event. progression of periodontitis by some of the markers ascertained or by their interactive effects.

Parodontitida je chron. zánětlivé postižení parodontu iniciované G- anaerobními bakteriemi dentálního plaku s cca 3-krát vyšším rizikem u diabetiků ve srovnání s nediabetickou populací, pravděpodobně v důsledku rozdílné imuno-/zánětlivé reaktivity na bakteriální osídlení parodontu při hyperglykémii. Navrhovaný projekt řeší v ČR dosud nesystematicky zmapovanou problematiku prevalence, typu a závažnosti parodontálního postižení u diabetiků a jeho vztahu k zákl. onemocnění. Hodláme vyšetřit parodontální status (klin. indexy + RTG), mikrobiální kolonizaci parodontu (DNA diagnostický kit pro 7 obvyklých parodont. patogenů), potenciální genetické rizikové faktory (30–45 variant) a koncentrace 7 vybraných solubilních markerů zánětu a kostní resorpce (ELISA). Studie zahrnuje analýzu„kontroly x případy“ (tj. ne- vs. diabetici, cca 400 osob) a prospektivní sledování diabetické kohorty s cílem predikovat event. progresi parodontitidy některými ze stanovovaných markerů nebo jejich příp. interakčního efektu.

• MeSH
• biologické markery MeSH
• diabetes mellitus 1. typu komplikace   MeSH
• diabetes mellitus 2. typu komplikace   MeSH
• ELISA MeSH
• nemoci parodontu diagnóza   genetika   mikrobiologie   MeSH
• parodontitida prevence a kontrola   MeSH
• zubní plak patologie   MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• diabetologie
• zubní lékařství
• otorinolaryngologie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

AIMS: The objective of the study was to measure one of the circulating Advanced Glycation End Products (AGEs) - Nε-(carboxymethyl)lysine (CML) - in a case-control study (n = 307) of pregnant women with gestational diabetes mellitus (GDM) and physiological pregnancies and to ascertain the factors contributing to CML levels and the potential relevance of CML for selected perinatal and postpartum outcomes. METHODS: All subjects underwent oGTT between 24th and 30th week of gestation and GDM was diagnosed according to WHO criteria. CML was determined by ELISA using commercial kit. RESULTS: Unadjusted and plasma protein adjusted CML levels were significantly higher in women with GDM compared to healthy controls (P = 0.00043 and P = 1x10(-5), respectively, Mann-Whitney). CML was significantly inversely correlated with both pre- and mid-gestational BMI, however, differences between GDM and control group remained significant even after adjustment for BMI. CML levels correlated with 1-h and 2-h post-load glycaemia during oGTT. CONCLUSION: In conclusion, we found statistically significantly higher protein- and BMI-normalised CML levels measured during 24-30th week of gestation in women with GDM compared to healthy pregnant controls. Further studies are warranted to comprehensively asses the spectrum of AGEs in GDM and their relevance to future metabolic health of mother and offspring.

• MeSH
• časná diagnóza MeSH
• dospělí MeSH
• druhý trimestr těhotenství krev   MeSH
• gestační diabetes krev   MeSH
• index tělesné hmotnosti MeSH
• krevní glukóza metabolismus   MeSH
• lidé MeSH
• lysin analogy a deriváty   metabolismus   MeSH
• porucha glukózové tolerance diagnóza   MeSH
• těhotenství MeSH
• výsledek těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

OBJECTIVE: Dental caries is a multifactorial, infectious disease where genetic predisposition plays an important role. Insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) has very recently been associated with caries in Polish children. The aim of this study was to analyze ACE I/D polymorphism in a group of caries-free children versus subjects affected by dental caries in the Czech population. MATERIALS AND METHODS: In this case-control study, 182 caries-free children (with decayed/missing/filled teeth, DMFT = 0), 561 subjects with dental caries (DMFT ≥1) aged 13-15 years and 220 children aged 2-6 years with early childhood caries (ECC, dmft ≥1) were included. Genotype determination of ACE I/D polymorphism in intron 16 was based on the TaqMan method. RESULTS: Although no significant differences in the allele or genotype frequencies between the caries-free children and those affected by dental caries were observed, statistically significant differences between the children with DMFT = 0 and the subgroup of 179 patients with high caries experience (DMFT ≥4; p < 0.01 and p < 0.05, respectively) were detected. The comparison of DD versus II+ID genotype frequencies between the patients with DMFT ≥1 or DMFT ≥4 and healthy children also showed significant differences (31.5% or 35.6% vs. 23.6%, p < 0.05 or p < 0.01, respectively). A gender-based analysis identified a significant difference in the DD versus II+ID genotype frequencies only in girls (p < 0.05). In contrast, no significant association of ACE I/D polymorphism with ECC in young children was found (p > 0.05). CONCLUSIONS: ACE I/D polymorphism may be associated with caries in permanent but not primary dentition, especially in girls in the Czech population.

• MeSH
• alely MeSH
• angiotensin konvertující enzym genetika   MeSH
• dentice trvalá * MeSH
• DMF Index MeSH
• genetická predispozice k nemoci genetika   MeSH
• genetické asociační studie MeSH
• genotyp MeSH
• introny MeSH
• lidé MeSH
• mladiství MeSH
• mutace INDEL * MeSH
• polymorfismus genetický * MeSH
• předškolní dítě MeSH
• sexuální faktory MeSH
• studie případů a kontrol MeSH
• zubní kaz epidemiologie   genetika   MeSH
• zuby mléčné * imunologie   mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH

OBJECTIVE: Dental caries is one of the most frequent multifactorial diseases. Among the numerous factors influencing the risk of caries, genetics plays a substantial role, with heritability ranging from 40 to 60%. Gene variants affecting taste preference and glucose transport were recently associated with caries risk. The aim of this study was to analyze two common polymorphisms in the sweet taste receptor (TAS1R2) and glucose transporter (GLUT2) genes in children with dental caries and healthy controls in the Czech population. METHODS: A total of 637 unrelated Caucasian children, aged 11-13 years, were included in this case-control study. One hundred and fifty-five subjects were caries-free (with decayed/missing/filled teeth, DMFT = 0) and 482 children were caries-affected (DMFT ≥ 1). The TAS1R2 (Ile191Val, rs35874116) and GLUT2 (Thr110Ile, rs5400) genotypes were determined using the 5' nuclease TaqMan® assay for allelic discrimination. RESULTS: Compared with subjects with the common Thr allele, carriers of the Ile allele of GLUT2 had significantly more frequently dental caries (p < 0.05, OR = 1.639, 95% CI: 1.089-2.466). Similarly, children with the Val allele for the TAS1R2 Ile191Val polymorphism were more frequently affected by caries than children who carried the Ile allele (p < 0.05, OR = 1.413, 95% CI: 1.014-1.969). In contrast, no significant associations between GLUT2 and/or TAS1R2 polymorphisms and fillings were found, but allele frequencies of the TAS1R2 variant were marginally significantly different between children with DMFT = 0 and DMFT ≥1 (p = 0.053, OR = 1.339, 95% CI: 0.996-1.799). However, no significant interaction between both genes and risk of dental caries was found. CONCLUSIONS: In conclusion, GLUT2 and TASR1 polymorphisms may influence the risk of caries in the Czech population.

• MeSH
• dítě MeSH
• DMF Index MeSH
• extrakce zubů statistika a číselné údaje   MeSH
• frekvence genu genetika   MeSH
• genetická variace genetika   MeSH
• genotyp MeSH
• guanin MeSH
• indexy plaku MeSH
• isoleucin genetika   MeSH
• jednonukleotidový polymorfismus genetika   MeSH
• lidé MeSH
• mladiství MeSH
• náchylnost k zubnímu kazu genetika   MeSH
• parodontální index MeSH
• polymorfismus genetický genetika   MeSH
• přenašeč glukosy typ 2 genetika   MeSH
• receptory spřažené s G-proteiny genetika   MeSH
• studie případů a kontrol MeSH
• threonin genetika   MeSH
• thymin MeSH
• trvalá zubní náhrada statistika a číselné údaje   MeSH
• valin genetika   MeSH
• zubní kaz genetika   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Česká republika MeSH

OBJECTIVES: Gastroesophageal reflux (GERD) is a one of the major public health problem that can lead to reflux esophagitis (RE), Barrett's esophagus (BE), and esophageal adenocarcinoma (EAC). The aim of our study was to determine the impact of IL-1 gene polymorphisms on the development of GERD, RE and BE. METHODS: Three hundred and thirty-three Czech patients with gastroesophageal reflux and 165 healthy controls were included in this case-control study. Four polymorphisms in the genes of the IL-1 cluster [IL-1A(-889C/T), IL-1B(-511C/T), IL-1B(+3953C/T), and IL-1RN(VNTR)] were analyzed. RESULTS: Significant differences were found in IL-1RN 1/2 genotype between patients with GERD/RE and controls and in IL-1B+3953 T allele between patients with BE and healthy subjects. In addition, complex analysis revealed differences in IL-1 haplotype frequencies between the groups. Specifically, the haplotype TCCL was significantly more frequent (p = 0.016) in GERD patients than in controls and the haplotype CCCL more frequent (p = 0.008) in RE patients than in controls. However, in patients with BE, frequency of haplotype TCTL was lower (p = 0.05) and haplotypes CTCL and TCCL were higher (p = 0.03 and p = 0.02) in comparison with the controls. CONCLUSIONS: Our results suggest that IL-1 haplotypes may be associated with susceptibility to GERD, RE and BE.

• MeSH
• Barrettův syndrom genetika   imunologie   MeSH
• dospělí MeSH
• frekvence genu MeSH
• gastroezofageální reflux genetika   imunologie   MeSH
• genetické asociační studie MeSH
• genotyp MeSH
• haplotypy MeSH
• interleukin-1 genetika   MeSH
• lidé středního věku MeSH
• lidé MeSH
• multigenová rodina imunologie   MeSH
• studie případů a kontrol MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

• Publikační typ
• abstrakt z konference MeSH

BACKGROUND/AIMS: Complex interplay of genetic and (patho)physiological factors influence availability of nitric oxide during the development and progression of diabetic complications. We assessed predictive value of commonly studied methylated asymmetric and symmetric dimethylarginines (ADMA and SDMA) and selected single nucleotide polymorphisms (SNPs) in dimethylarginine dimethylaminohydrolase (DDAH) 1 and 2 genes for the progression of diabetic nephropathy (DN). METHODS: A total of 341 type 1 and type 2 diabetes patients with variable degree of kidney disease were included at baseline. Plasma levels of ADMA, SDMA and L-arginine were measured and six tagging SNPs in DDAH1 and 2 were determined. Progression of DN was defined as a transition from any given stage to a more advanced stage of albuminuria. Competing risk analysis was applied. RESULTS: Plasma levels of ADMA and SDMA significantly correlated with GFR. No significant genotype-phenotype relationship was ascertained for ADMA and DDAH variants, but SNP rs805304 exhibited marginally significant association with DN. ADMA, SDMA and L-arginine/ADMA ratio standardised to GFR were identified as significant predictors of DN progression but not GFR decline using multivariate competing risk analysis. CONCLUSIONS: In our study we confirmed potentially significant role of ADMA and SDMA for the assessment of risk of DN progression in European diabetic population.

• MeSH
• amidohydrolasy genetika   MeSH
• arginin analogy a deriváty   krev   genetika   MeSH
• diabetes mellitus 1. typu komplikace   MeSH
• diabetes mellitus 2. typu komplikace   MeSH
• diabetické nefropatie * krev   etiologie   genetika   MeSH
• dospělí MeSH
• jednonukleotidový polymorfismus genetika   MeSH
• lidé středního věku MeSH
• lidé MeSH
• metylace MeSH
• multivariační analýza MeSH
• následné studie MeSH
• prediktivní hodnota testů MeSH
• prognóza MeSH
• progrese nemoci * MeSH
• průřezové studie MeSH
• reprodukovatelnost výsledků MeSH
• rizikové faktory MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

• Publikační typ
• abstrakt z konference MeSH

• Publikační typ
• abstrakt z konference MeSH

• Publikační typ
• abstrakt z konference MeSH