Assembly
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Archives of physical medicine and rehabilitation, ISSN 0003-9993
svazky ; 28 cm
Ve dnech 11. - 12. 3. 2024 proběhlo v Zandvoort aan Zee v Nizozemsku roční setkání zástupců mezinárodního projektu „Union and National Capacity Building 4 IntegraTED Surveillance“ (zkráceně UNITED4Surveillance). Jedná se o tříletý projekt (na období 2023-2025) společných evropských aktivit spadající pod EU4Health Joint Action. Za Českou republiku se projektu účastní Státní zdravotní ústav. Článek je stručným souhrnem stávající situace projektu.
On March 11–12, 2024, the annual meeting of representatives of the international project "Union and National Capacity Building 4 IntegraTED Surveillance" (abbreviated as UNITED4Surveillance) took place in Zandvoort aan Zee, the Netherlands. This is a three-year project (for the period 2023–2025) of joint European activities falling under the EU4Health Joint Action. The National Institute of Public Health is participating in the project on behalf of the Czech Republic. The article is a brief summary of the current situation of the project.
sv.
- MeSH
- celosvětové zdraví MeSH
- mezinárodní spolupráce MeSH
- Světová zdravotnická organizace MeSH
- Publikační typ
- periodika MeSH
Chromatin Assembly Factor 1 (CAF-1) is a major nucleosome assembly complex which functions particularly during DNA replication and repair. Here we studied how the nucleosome landscape changes in a CAF-1 mutant in the model plant Arabidopsis thaliana. Globally, most nucleosomes were not affected by loss of CAF-1, indicating the presence of efficient alternative nucleosome assemblers. Nucleosomes that we found depleted in the CAF-1 mutant were enriched in non-transcribed regions, consistent with the notion that CAF-1-independent nucleosome assembly can compensate for loss of CAF-1 mainly in transcribed regions. Depleted nucleosomes were particularly enriched in proximal promoters, suggesting that CAF-1-independent nucleosome assembly mechanisms are often not efficient upstream of transcription start sites. Genes related to plant defense were particularly prone to lose nucleosomes in their promoters upon CAF-1 depletion. Reduced nucleosome occupancy at promoters of many defense-related genes is associated with a primed gene expression state that may considerably increase plant fitness by facilitating plant defense. Together, our results establish that the nucleosome landscape in Arabidopsis is surprisingly robust even in the absence of the dedicated nucleosome assembly machinery CAF-1 and that CAF-1-independent nucleosome assembly mechanisms are less efficient in particular genome regions.
- MeSH
- Arabidopsis genetika imunologie metabolismus MeSH
- chromatin genetika MeSH
- faktor 1 pro uspořádání chromatinu genetika metabolismus MeSH
- imunita rostlin genetika MeSH
- mutace MeSH
- nukleozomy genetika metabolismus MeSH
- oprava DNA genetika MeSH
- počátek transkripce MeSH
- promotorové oblasti (genetika) genetika MeSH
- replikace DNA genetika MeSH
- restrukturace chromatinu genetika MeSH
- sekvenční analýza DNA MeSH
- Publikační typ
- časopisecké články MeSH
1st ed. XVI, 80 s. : fot., lit. ; 24 cm
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- environmentální vědy
- veřejné zdravotnictví
- NLK Publikační typ
- publikace WHO
163 s. : tab.
- MeSH
- psychiatrie MeSH
- psychologie MeSH
- služby péče o duševní zdraví organizace a řízení MeSH
- zdravotnické plánování - směrnice MeSH
- zdravotnické služby - potřeby a požadavky MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- psychologie, klinická psychologie
- management, organizace a řízení zdravotnictví
- psychiatrie
- NLK Publikační typ
- publikace WHO
Due to the high number of drug-resistant HIV-1 mutants generated by highly active antiretroviral therapy (HAART), there is continuing demand for new types of inhibitors. Both the assembly of the Gag polyprotein into immature and mature HIV-1 particles are attractive candidates for the blocking of the retroviral life cycle. Currently, no therapeutically-used assembly inhibitor is available. One possible explanation is the lack of a reliable and simple assembly inhibitor screening method. To identify compounds potentially inhibiting the formation of both types of HIV-1 particles, we developed a new fluorescent high-throughput screening assay. This assay is based on the quantification of the assembly efficiency in vitro in a 96-well plate format. The key components of the assay are HIV-1 Gag-derived proteins and a dual-labelled oligonucleotide, which emits fluorescence only when the assembly of retroviral particles is inhibited. The method was validated using three (CAI, BM2, PF74) reported assembly inhibitors.
- MeSH
- genové produkty gag metabolismus MeSH
- HIV infekce farmakoterapie virologie MeSH
- HIV-1 účinky léků fyziologie MeSH
- látky proti HIV farmakologie MeSH
- lidé MeSH
- preklinické hodnocení léčiv metody MeSH
- rychlé screeningové testy metody MeSH
- sestavení viru účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
