• MeSH
• rekombinace genetická MeSH
• replikace DNA MeSH
• Publikační typ
• kongresy MeSH

• Konspekt
• Biologické vědy
• NLK Obory
• biologie
• embryologie a teratologie
• genetika, lékařská genetika

• MeSH
• DNA virů biosyntéza   MeSH
• kuřecí embryo MeSH
• replikace viru MeSH
• virus vakcinie MeSH
• Check Tag
• kuřecí embryo MeSH

• MeSH
• eukaryotické buňky MeSH
• replikace DNA MeSH
• Publikační typ
• přehledy MeSH

• MeSH
• mikrobiální genetika MeSH
• mutageneze MeSH
• replikace DNA MeSH
• Publikační typ
• kongresy MeSH

• Konspekt
• Mikrobiologie
• NLK Obory
• mikrobiologie, lékařská mikrobiologie
• embryologie a teratologie
• genetika, lékařská genetika

The replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared nucleoside and nucleotide analogues that serve as markers of newly synthesized DNA. These analogues are incorporated into the DNA during DNA replication, and are subsequently visualized. Several methods are used for their detection, including the highly popular click chemistry. This review aims to provide the readers with basic information about the various possibilities of the detection of replication activity using nucleoside and nucleotide analogues, and to show the strengths and weaknesses of those different detection systems, including click chemistry for microscopic studies.

• MeSH
• click chemie MeSH
• DNA chemie   genetika   MeSH
• halogenace MeSH
• hybridizace in situ MeSH
• imunohistochemie MeSH
• izotopové značení MeSH
• měď chemie   MeSH
• nukleosidy chemie   MeSH
• nukleotidy chemie   MeSH
• radionuklidy MeSH
• replikace DNA * MeSH
• výzkum MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

• MeSH
• antimetabolity MeSH
• DNA virů MeSH
• replikace viru MeSH
• RNA virová MeSH
• virové receptory MeSH
• Publikační typ
• přehledy MeSH

RECQ5 belongs to the RecQ family of DNA helicases. It is conserved from Drosophila to humans and its deficiency results in genomic instability and cancer susceptibility in mice. Human RECQ5 is known for its ability to regulate homologous recombination by disrupting RAD51 nucleoprotein filaments. It also binds to RNA polymerase II (RNAPII) and negatively regulates transcript elongation by RNAPII. Here, we summarize recent studies implicating RECQ5 in the prevention and resolution of transcription-replication conflicts, a major intrinsic source of genomic instability during cancer development.

• MeSH
• DNA genetika   metabolismus   MeSH
• genetická transkripce genetika   MeSH
• helikasy RecQ genetika   metabolismus   fyziologie   MeSH
• lidé MeSH
• nestabilita genomu MeSH
• replikace DNA MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

The astonishing survival abilities of Vicia faba, one the earliest domesticated plants, are associated, among other things, to the highly effective replication stress response system which ensures smooth cell division and proper preservation of genomic information. The most crucial pathway here seems to be the ataxia telangiectasia-mutated kinase (ATM)/ataxia telangiectasia and Rad3-related kinase (ATR)-dependent replication stress response mechanism, also present in humans. In this article, we attempted to take an in-depth look at the dynamics of regeneration from the effects of replication inhibition and cell cycle checkpoint overriding causing premature chromosome condensation (PCC) in terms of DNA damage repair and changes in replication dynamics. We were able to distinguish a unique behavior of replication factors at the very start of the regeneration process in the PCC-induced cells. We extended the experiment and decided to profile the changes in replication on the level of a single replication cluster of heterochromatin (both alone and with regard to its position in the nucleus), including the mathematical profiling of the size, activity and shape. The results obtained during these experiments led us to the conclusion that even "chaotic" events are dealt with in a proper degree of order.

• MeSH
• chromozomy rostlin genetika   MeSH
• fluorescence MeSH
• fyziologický stres * MeSH
• heterochromatin metabolismus   MeSH
• kinetika MeSH
• meristém fyziologie   MeSH
• oprava DNA * MeSH
• poškození DNA MeSH
• regenerace fyziologie   MeSH
• replikace DNA * MeSH
• Vicia faba fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• DNA-dependentní DNA-polymerasy fyziologie   MeSH
• eukaryotické buňky fyziologie   MeSH
• prokaryotické buňky fyziologie   MeSH
• replikace DNA * genetika   MeSH