Chromosome stability is conditioned by functional chromatin structure of chromosome ends - telomeres. Organisation and regulation of telomere maintenance represent a complex process whose details still remain enigmatic, especially in plants. Several telomere-binding or telomere-associated proteins and distinct epigenetic marks have been shown to influence telomere length and telomerase activity. HMGB proteins play important role in dynamic changes of chromatin structure and are involved in regulation of cellular processes of key importance, such as replication, transcription, recombination and DNA-repair. HMGB proteins in plants are more diversified than in other eukaryotes. Here, we summarise the roles of plant HMGB proteins in regulation of chromatin structure and dynamics and report on the newly identified role of AtHMGB1 protein in the regulation of plant telomere length. Astonishingly, contrary to mice mHMGB1 homologue, AtHMGB1 does not affect telomerase activity and AtHMGB1 loss or overexpression does not cause any obvious changes in chromatin architecture.

• MeSH
• Arabidopsis metabolismus   MeSH
• chromatin chemie   metabolismus   MeSH
• epigenomika MeSH
• protein HMGB1 metabolismus   MeSH
• proteiny HMGB metabolismus   MeSH
• proteiny huseníčku metabolismus   MeSH
• telomery metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

• MeSH
• chromatin MeSH
• DNA-topoisomerasy typu II MeSH
• domény HMG-Box MeSH
• genetická transkripce MeSH
• geny p53 MeSH
• protein HMGB1 izolace a purifikace   MeSH
• proteiny HMGB MeSH
• Publikační typ
• vysokoškolské kvalifikační práce MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• biologie

HMGB proteins are members of the High Mobility Group (HMG) superfamily, possessing a unique DNA-binding domain, the HMG-box, which can bind non-B-type DNA structures (bent, kinked and unwound) with high affinity, and also distort DNA by bending/looping and unwinding. HMGBs (there are four HMGBs in mammals, HMGB1-4) are highly abundant and ubiquitously expressed non-histone proteins, acting as DNA chaperones influencing multiple processes in chromatin such as transcription, replication, recombination, DNA repair and genomic stability. Although HMGB1 is a nuclear protein, it can be secreted into the extracellular milieu as a signaling molecule when cells are under stress, in particular, when necrosis occurs. Mammalian HMGBs contain two HMG-boxes arranged in tandem, share more than 80% identity and differ in the length (HMGB1-3) or absence (HMGB4) of the acidic C-tails. The acidic tails consist of consecutive runs of only Glu/Asp residues of various length, and modulate the DNA-binding properties and functioning of HMGBs. HMGBs are subject to post-translational modifications which can fine-tune interactions of the proteins with DNA/chromatin and determine their relocation from the nucleus to the cytoplasm and secretion. Association of HMGBs with chromatin is highly dynamic, and the proteins affect the chromatin fiber as architectural factors by transient interactions with nucleosomes, displacement of histone H1, and facilitation of nucleosome remodeling and accessibility of the nucleosomal DNA to transcription factors or other sequence-specific proteins.

• MeSH
• chromatin metabolismus   MeSH
• DNA metabolismus   MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• posttranslační úpravy proteinů MeSH
• proteiny HMGB chemie   genetika   metabolismus   MeSH
• regulace genové exprese MeSH
• sekvence aminokyselin MeSH
• vazba proteinů MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

Intraperitoneální podání peritoneálních dialyzačních (PD) roztoků s sebou nese i zvýšenou intraperitoneální nálož glukózy a jejích degradačních produktů (GDP), což přímo ovlivňuje systémové hladiny konečných produktů pokročilé glykace (AGEs). Plazmatické hladiny solubilního receptorů pro AGE (sRAGE) ani jeho prozánětlivých ligand extracellular newly identified receptor for AGE (EN-RAGE) a high mobility group box-1 proteinu (HMGB-1) nebyly zatím u PD detailně zkoumány. Proto jsme v návaznosti na naši předchozí studii reanalyzovali vyšetřovaný soubor s cílem ozřejmit vztah sRAGE a jeho prozánětlivých ligand k dalším laboratorním a klinickým parametrům. Soubor nemocných a metody: skupina PD pacientů (n = 33), věk 59 (20-84) let; skupina zdravých dobrovolníků (HV, n = 20) věk 41,5 (23-56) let a skupina CKD pacientů stadia 3-5/5 (n = 20). věk 70 (39-81) let. Solubilní RAGE, EN-RAGE a HMGB-1 byly měřeny pomocí standardních ELISA kitů dle protokolu výrobce. Výsledky: PD pacienti vykazovali vyšší plazmatické hladiny sRAGE a nižší hladiny HMGB-1 [ng/ml] ve srovnání s HV [2,1 (0,7-6,4) Vs. 1,0 (0,7-1,9), p < 0,001; 1,6 (0,5-4,9) vs. 2,1 (0,9-25,4), p < 0,05] a nižší hladiny EN-RAGE ve srovnání s CKD [36,3 (6,7-194,0) vs. 65,6 (13,9-160,0), ng/ml, p < 0,05]. Pacienti s vyšším/rychlejším peritoneálním transportem (D/Pcr > 0,65) vykazovali vyšší plazmatické koncentrace sRAGE než pacienti s nízkým/pomalým transportem, trend však nedosáhl statistické významnosti \.2,3 (1,5-6,4) vs. 1,8 (1,1-5,0) ng/ml, p = 0,056]. Pacienti s CRP vyšším než medián vykazovali vyšší plazmatické hladiny obou prozánětlivých ligand HMGB-1 a EN-RAGE, [1,7 (0,8-4,9) vs. 1,4 (0,7-2,9); 43,1 (8,8-112,5) vs. 27.7 (0-194,0)] (ng/ml), p < 0,05 pro oba ligandy. U HMGB-1 byly pozorovány vyšší koncentrace v PD effluentu než v plazmě a nebyla mezi nimi - na rozdíl od sRAGE a EN-RAGE - pozorována vzájemná korelace. Korelace plazmatických koncentrací HMGB-1 a BMI jsme prokázali jak u PD (r = 0,364, p < 0,05), tak u CKD (r = 0,559, p < 0,05) pacientů. Nenašli jsme vztah mezi plazmatickými koncentracemi EN-RAGE a reziduální renální funkcí u PD ani CKD pacientů. U subjektů všech tří podskupin (PD a CKD pacientů i zdravých dobrovolníků) byla zjištěna signifikantní pozitivní korelace mezi hladinami EN-RAGE a HMGB-1. Závěr: V souladu s předešlými studiemi jsme prokázali vyšší plazmatické hladiny sRAGE u PD a CKD pacientů ve srovnání se zdravými dobrovolníky, design naší studie však neumožňuje rozlišit, zda je to dáno sníženou renální clearance nebo nadprodukcí sRAGE ve snaze snížit toxické efekty AGEs akumulovaných u uremických pacientů. Při peritoneální dialýze jsou plazmatické hladiny sRAGE a jeho prozánětlivých ligand zřejmě ovlivněny nejen stupněm mikrozánětu, ale i dávkou dialýzy, peritoneálním transportem, BMI potažmo stavenri nutrice a v případě sRAGE a HMGB-1 také reziduální renální funkcí. HMGB-1 se ukazuje jako velice citlivý ukazatel cytotoxického působení peritoneálního dialyzačního roztoku. Vzájemná korelace hladin HMGB-1 a EN-RAGE demonstrovaná u všech zkoumaných podskupin pacientů podporuje představu, že biologická aktivita těchto prozánětlivých ligand spolu úzce souvisí.

The intraperitoneal administration of peritoneal dialysis (PD) solutions is associated with higher intraperitoneal load of glucose and its degradation products (GDP) which directly affects the advanced glycation end products (AGEs) systemic levels. The plasma levels of soluble receptor for AGE (sRAGE) and its proinflammatory ligands, the extracellular newly identified receptor for AGE (EN-RA GE) and high mobility group box-1 protein (HMGB-1), were not studied in PD patients in detail as yet. As a follow-up to our previou s study we carried out a reanalysis of data with the aim to find a relationship between sRAGE and its proinflammatory ligands and other laboratory and clinical parameters. Patients and methods: group of PD patients (n=33), aged 59 (20-84) years; group of healthy volunteers (HV) (n=20), aged 41.5 (23-56) years; and group of chronic kidney disease patients (CKD) stage 3-5 (n=20), aged 70 (39-81) years. Soluble RAGE, EN-RAG E and HMGB-1 were measured using standard ELISA (enzyme linked immunosorbent assay) kits, according to the manufacturer ́s protocols. Results: PD patients showed higher plasma sRAGE and lower HMGB-1 [ng/mL] levels compared to HV [2.1 (0.7-6.4) vs. 1.0 (0.7-1.9), p < 0.001; 1.6 (0.5-4.9) vs. 2.1 (0.9-25.4), p < 0.05] and lower EN-RAGE levels compared to CKD [36.3 (6.7-194.0) vs. 65.6 (13. 9- 160.0) ng/mL, p < 0.05]. Patients with higher/faster peritoneal transport characteristics (D/Pcr > 0.65) demonstrated higher sR AGE plasma levels compared to low/slow peritoneal transport but the difference did not reach statistical significance [2.3 (1.5-6.4 ) vs. 1.8 (1.1-5.0) ng/mL, p = 0.056]. Patients with CRP above median demonstrated higher plasma HMGB-1 and EN-RAGE levels, [1.7 (0.8- 4.9) vs. 1.4 (0.7-2.9); 43.1 (8.8-112.5) vs. 27.7 (0-194.0)] [ng/mL], p < 0.05 for both. HMGB-1 PD effluent levels were higher than its plasma levels and they did show mutual correlation, contrary to sRAGE and EN-RAGE plasma and effluent levels. We confirmed correlation between plasma HMGB-1 levels and BMI both in PD (r=0.364, p < 0.05) and CKD (r=0.559, p < 0.05) patients. We did not find any relationship between plasma EN-RAGE levels and residual renal function in PD and CKD patients. In subjects of all the three groups studied (PD, CKD and HV) a significant positive correlation between EN-RAGE and HMGB-1 plasma levels was demonstrated Conclusion: In consent with previous studies we confirmed higher sRAGE plasma levels in PD and CKD compared to healthy volunteers. Design of our study does not enable to determine whether it is due to the decreased renal clearance or sRAGE overproduction (in an effort to counteract the toxic effects of AGEs accumulated in uremic patients). Soluble RAGE and its proinflammatory ligands plasma levels in PD seem to be influenced by the degrese of microinflammation, dose of dialysis, perito neal transport, BMI/nutrition status and in the case of sRAGE and HMGB-1 by residual renal function as well. HMGB-1 seems to be a highly sensitive marker of cytotoxicity of peritoneal dialysis solution. Mutual correlation between HMGB-1 and EN-RAGE demonstrated in all subgroups studied supports the idea that the biological activity of these proinflammatory ligands is closely interrelated.

• Klíčová slova
• sRAGE,
• MeSH
• biologický transport MeSH
• C-reaktivní protein MeSH
• chronické selhání ledvin metabolismus   MeSH
• dialyzační roztoky * metabolismus   MeSH
• dospělí MeSH
• ELISA MeSH
• index tělesné hmotnosti MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• peritoneální dialýza MeSH
• produkty pokročilé glykace * krev   metabolismus   MeSH
• protein HMGB1 * krev   MeSH
• receptory imunologické krev   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• statistika jako téma MeSH
• studie případů a kontrol MeSH
• zánět patofyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH

Massive bleeding causes approximately 50% of deaths in patients with major trauma. Most patients die within 6 h of injury, which is preventable in at least 10% of cases. For these patients, early activation of the massive transfusion protocol (MTP) is a critical survival factor. With severe trauma, high-mobility group box 1 (HMGB-1, i.e., amphoterin) is released into the blood, and its levels correlate with the development of a systemic inflammatory response, traumatic coagulopathy, and fibrinolysis. Previous work has shown that higher levels of HMGB-1 are associated with a higher use of red blood cell transfusions. We conducted a retrospective analysis of previous prospective single-center study to assess the value of admission HMGB-1 levels in predicting activation of MTP in the emergency department. From July 11, 2019, to April 23, 2022, a total of 104 consecutive adult patients with severe trauma (injury severity score > 16) were enrolled. A blood sample was taken at admission, and HMGB-1 was measured. MTP activation in the emergency department was recorded in the study documentation. The total amount of blood products and fibrinogen administered to patients within 6 h of admission was monitored. Among those patients with massive bleeding requiring MTP activation, we found significantly higher levels of HMGB-1 compared to patients without MTP activation (median [interquartile range]: 84.3 μg/L [34.2-145.9] vs. 21.1 μg/L [15.7-30.4]; p < 0.001). HMGB-1 level showed good performance in predicting MTP activation, with an area under the receiver operating characteristic curve of 0.84 (95% CI 0.75-0.93) and a cut-off value of 30.55 μg/L. HMGB-1 levels correlated significantly with the number of red blood cell units (rs [95% CI] 0.46 [0.28-0.61]; p < 0.001), units of fresh frozen plasma (rs 0.46 [0.27-0.61]; p < 0.001), platelets (rs 0.48 [0.30-0.62]; p < 0.001), and fibrinogen (rs 0.48 [0.32-0.62]; p < 0.001) administered in the first 6 h after hospital admission. Admission HMGB-1 levels reliably predict severe bleeding requiring MTP activation in the emergency department and correlate with the amount of blood products and fibrinogen administered during the first 6 h of hemorrhagic shock resuscitation.Trial registration: NCT03986736. Registration date: June 4, 2019.

• MeSH
• biologické markery krev   MeSH
• dospělí MeSH
• krevní transfuze * metody   MeSH
• krvácení * krev   terapie   etiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• prospektivní studie MeSH
• protein HMGB1 * krev   MeSH
• rány a poranění * krev   terapie   komplikace   MeSH
• retrospektivní studie MeSH
• senioři MeSH
• skóre závažnosti úrazu MeSH
• urgentní služby nemocnice MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The HMG-box domain of approximately 75 amino acid residues was originally identified as the domain that mediates the DNA-binding of chromatin-associated high-mobility group (HMG) proteins of the HMGB type. In the last few years, HMG-box domains have been found in various DNA-binding proteins including transcription factors and subunits of chromatin-remodeling complexes. HMG-box domains mediate either non-sequence-specific (e.g., HMGB-type proteins) or sequence-specific (e.g., transcription factors) DNA binding. Both types of HMG-box domains bind non-B-type DNA structures (bent, kinked and unwound) with high affinity. In addition, HMG-box domains are involved in a variety of protein-protein interactions. Here, we have examined the human and plant genomes for genes encoding HMG-box domains. Compared to plants, human cells contain a larger variety of HMG-box proteins. Whereas in humans transcription factors are the most divergent group of HMG-box proteins, in plants the chromosomal HMGB-type proteins are most variable.

• MeSH
• DNA vazebné proteiny MeSH
• domény HMG-Box MeSH
• financování organizované MeSH
• genom lidský MeSH
• genom rostlinný MeSH
• jaderné proteiny MeSH
• lidé MeSH
• proteiny Drosophily MeSH
• proteiny HMGB MeSH
• restrukturace chromatinu MeSH
• transkripční faktory MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

Intraperitoneal glucose degradation products (GDP) load influences systemic advanced glycation end products (AGEs) but the effects on soluble receptor for AGEs (s-RAGE) and its proinflammatory ligands: extracellular newly identified receptor for advanced glycation end-products binding protein(EN-RAGE) and high mobility group box-1 protein (HMGB-1) are unknown. We aimed to compare plasma and peritoneal s-RAGE, EN-RAGE and HMGB-1 between three peritoneal dialysis (PD) prescription regimens with different intraperitoneal GDP loads. High GDP load (glucose-lactate PD fluid, D; N = 8) was compared with a low (glucose-bicarbonate/lactate with icodextrin for overnight dwell, E; N = 9) and a very low GDP load (glucose-bicarbonate/lactate, P; N = 16). D group demonstrated higher plasma EN-RAGE, 77.8 ng/mL, vs. both E, 11.2, P < 0.001 and P, 27.0, P < 0.001 as well as higher plasma HMGB-1, 2.2 ng/mL vs. both E, 1.1, P < 0.01 and P, 1.5, P < 0.01. Plasma s-RAGE, which did not differ between D, E and P, correlated with its effluent levels. Patients with faster peritoneal transport (D/Pcr > 0.65) tended to have higher plasma s-RAGE compared to slow transporters (2300 vs. 1762 pg/mL, P = 0.056). Peritoneal clearance of s-RAGE and EN-RAGE was higher with E compared to both D and P (P < 0.001 resp. P < 0.01). Subgroup of PD patients with CRP above median demonstrated higher plasma HMGB-1 and EN-RAGE, P < 0.05 for both. A lower intraperitoneal GDP load is associated with decreased plasma levels of EN-RAGE and HMGB-1. Peritoneal transport, microinflammation and the capability of icodextrin to increase peritoneal clearance of middle molecular weight substances might also exert an effect on plasma s-RAGE and its proinflammatory ligands levels.

• MeSH
• biologický transport fyziologie   MeSH
• glukany aplikace a dávkování   MeSH
• glukosa aplikace a dávkování   metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• ligandy MeSH
• mediátory zánětu metabolismus   MeSH
• mladý dospělý MeSH
• peritoneální dialýza metody   MeSH
• peritoneum metabolismus   MeSH
• produkty pokročilé glykace metabolismus   MeSH
• protein HMGB1 metabolismus   MeSH
• receptory imunologické metabolismus   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

The binding of high-mobility group box-1 (HMGB-1) to the membrane receptor for advanced glycation end-products (mRAGE) is a key early mediator of non-infectious inflammation and its triggers include ischaemia/hypoxia. The effects of acute hypoxia on soluble RAGE (sRAGE) are unknown. Fourteen healthy adults (50 % women; 26.6+/-3.8 years) were assessed at baseline normoxia (T0), followed by four time-points (T90, 95, 100 and 180 min) over three hours of continuous normobaric hypoxia (NH, 4,450 m equivalent) and again 60 min after return to normoxia (T240). A 5-min exercise step test was performed during NH at T90. Plasma concentrations of HMGB-1, sRAGE VCAM-1, ICAM-1, VEGF IL-8 and IL-13 were measured using venous blood. Arterial and tissue oxygen saturations were measured using pulse oximetry (SpO(2)) and near-infrared spectroscopy (StO(2)), respectively. NH led to a significant reduction in SpO(2), StO(2), sRAGE and VEGF, which was compounded by exercise, before increasing to baseline values with normoxic restoration (T240). NH-exercise led to a paired increase in HMGB-1. sRAGE inversely correlated with HMGB-1 (r=-0.32; p=0.006), heart rate (r=-0.43; p=0.004) but was not linked to SpO(2) or StO(2). In conclusion, short-term NH leads to a fall in sRAGE and VEGF concentrations with a transient rise post NH-exercise in HMGB-1.

• MeSH
• alarminy krev   MeSH
• biologické markery krev   MeSH
• dospělí MeSH
• hypoxie krev   diagnóza   MeSH
• lidé MeSH
• mediátory zánětu krev   MeSH
• mladý dospělý MeSH
• prospektivní studie MeSH
• protein HMGB1 krev   MeSH
• receptor pro konečné produkty pokročilé glykace krev   MeSH
• spotřeba kyslíku fyziologie   MeSH
• vaskulární endoteliální růstový faktor A krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• pozorovací studie MeSH

BACKGROUND: Placental growth factor (PlGF), pregnancy-associated plasma protein-A (PAPP-A), soluble receptor for advanced glycation end products (sRAGE), extracellular newly identified receptor for RAGE binding protein (EN-RAGE) and high mobility group box 1 (HMGB-1) are novel biomarkers in chronic kidney disease (CKD). However, their clinical significance in acute kidney injury (AKI) is unknown. The aim of this cross-sectional study was to determine whether selected biomarkers are changed in AKI patients. METHODS: Serum PlGF, PAPP-A, sRAGE, EN-RAGE and HMGB-1 levels were assessed in 40 patients with AKI, 42 CKD 5 patients, 31 haemodialysis patients (HD) and 39 age-matched healthy controls. RESULTS: PAPP-A was elevated in AKI (20.6 ± 16.9 mIU/L) compared with controls (9.1 ± 2.3 mIU/L, p < 0.001). PlGF was not increased in AKI (11.7 ± 7.4 pg/mL) versus controls (8.5 ± 2.4 pg/mL, n.s.), as well as sRAGE was not elevated in AKI (2400 ± 1400 pg/mL) compared with controls (1760 ± 730 pg/mL, n.s), but was lower compared with CKD 5 (3200 ± 1500 pg/mL, p < 0.05); EN-RAGE was elevated in AKI 480 ± 450 ng/mL in comparison with controls (60 ± 62 ng/mL), CKD 5 (190 ± 120 ng/mL), and HD (120 ± 100 ng/mL), all p < 0.001. Similarly, HMGB-1 was increased in AKI (5.8 ± 7.5 ng/mL) versus controls (1.7 ± 1.4 ng/mL), CKD 5 (3.2 ± 3.1 ng/mL) and HD (2.5 ± 2.1 ng/mL), all p < 0.001.In AKI group, in multivariate regression analysis: PAPP-A levels were associated with transferrin (p <0.001), negatively with albumin (p < 0.01) and prealbumin (p < 0.05); PlGF levels were associated with C--reactive protein (p < 0.001). EN-RAGE levels were associated with ferritin (p < 0.01) and orosomucoid (p = 0.02), and HMGB-1 levels with leukocyte count (p < 0.01) and negatively with proteinuria (p = 0.02). CONCLUSIONS: In AKI patients, PAPP-A, EN-RAGE and HMGB1 are elevated, but sRAGE and PlGF are not increased. Whereas PAPP-A correlates with markers of nutrition; PlGF, EN-RAGE and HMGB-1 are related to inflammatory parameters.

• MeSH
• akutní poškození ledvin krev   epidemiologie   MeSH
• chronická renální insuficience krev   epidemiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• prevalence MeSH
• protein HMGB1 krev   MeSH
• průřezové studie MeSH
• receptory imunologické krev   MeSH
• reprodukovatelnost výsledků MeSH
• rizikové faktory MeSH
• senzitivita a specificita MeSH
• těhotenské proteiny krev   MeSH
• těhotenský plazmatický protein A analýza   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

BACKGROUND Acute kidney injury (AKI) is a common cause of organ failure in patients after major trauma and is associated with increased morbidity and mortality. Early identification of patients at risk enables the implementation of a bundle of supportive care, which reduces the incidence of AKI. The primary objective of our study was to investigate whether the levels of biomarkers on admission predicted the onset of early AKI in patients with serious injuries. MATERIAL AND METHODS This prospective observational study included 98 adult patients of both sexes with a serious injury (injury severity score >16). At admission, blood samples were taken, and creatinine, neutrophil gelatinase-associated lipocalin (NGAL), high mobility group box 1 (HMGB-1), and markers of rhabdomyolysis (creatine kinase, myoglobin) were evaluated. The patients were provided with standard resuscitation care, and the occurrence of AKI was monitored during the first 7 days after admission to the Intensive Care Unit, according to the Kidney Disease Improving Global Outcomes diagnostic criteria. RESULTS AKI occurred in 25 (25.5%) patients, in whom the admission levels of HMGB-1, NGAL, creatinine, and myoglobin were significantly higher than in non-AKI patients (48.3±98.4 vs 113.0±209.4 μg/L, P=0.006; 150.2±349.9 vs 181.4±152.2 μg/L, P=0.004; 83.1±20.8 vs 118.8±32.2 μmol/L, P<0.005; 2734.4±2214.5 vs 4182.3±2477.1 μg/L, P=0.008, respectively). Creatine kinase was 14.5±9.2 μkat/L in non-AKI patients and 13.7±7.9 μkat/L in AKI patients (P=0.916). CONCLUSIONS Admission levels of HMGB-1, NGAL, creatinine, and myoglobin predicted the risk of AKI in severely injured patients.

• MeSH
• akutní poškození ledvin * diagnóza   etiologie   MeSH
• biologické markery MeSH
• dospělí MeSH
• kreatinin MeSH
• kreatinkinasa MeSH
• lidé MeSH
• lipokalin-2 MeSH
• myoglobin * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• pozorovací studie MeSH