Two approaches to polymer surface nanostructuring by laser beam are discussed: (i) exposure of surface to a polarized excimer laser beam and (ii) scanning of doped polymer surface by a semiconductor laser. The effect of laser fluence, the angle of incident laser beam and its wavelength on the nanostructure formation is described. Nanostructured polymers are used as substrates for deposition of Au nanolayers and biocompatibility studies. The properties of deposited Au nanolayers are significantly influenced by surface structure and chemical nature of the used substrate. Polymer films doped with porphyrin were irradiated with laser and simultaneously mechanically scanned. By combination of these two techniques a regular periodic pattern was formed. The structural properties depend on scanning rate and laser intensity. Nanostructured polymer surfaces are promising substrates in electronics, optics and tissue engineering.

• Klíčová slova
• laserový svazek, interakce,
• MeSH
• biokompatibilní materiály MeSH
• biopolymery terapeutické užití   MeSH
• buněčné kultury MeSH
• lasery využití   MeSH
• nanostruktury MeSH
• nanotechnologie MeSH
• polymery terapeutické užití   MeSH
• povrchové vlastnosti MeSH
• tkáňové inženýrství MeSH
• tkáňové podpůrné struktury MeSH
• Publikační typ
• práce podpořená grantem MeSH

The present work provides an overview of the results of studies devoted to the use of two polymers, polyetheretherketone and ultrahigh molecular weight polyethylene in the field of tissue engineering. The effect of plasma modification and cathode sputtering of gold on polymer surface properties and especially on cytocompatibility of these polymers was described. Both modification steps lead to significant changes of surface properties, such as the chemical composition of the surface layer, wettability, roughness and surface morphology. These properties have a significant effect on the surface biocompatibility. Plasma modification has a beneficial effect on cell adhesion and proliferation depending on the duration of exposure. The duration of sputtering affects the size and stability of the gold nanostructures, isolated nanoclusters can partially be released into the biological solution and thus affect the cytocompatibility of the polymer.

• MeSH
• biokompatibilní materiály MeSH
• polymery * MeSH
• tkáňové inženýrství MeSH
• Publikační typ
• práce podpořená grantem MeSH

Carbon-based materials have emerged as promising candidates for a wide variety of biomedical applications, including tissue engineering. We have developed a simple but unique technique for patterning carbon-based substrates in order to control cell adhesion, growth and phenotypic maturation. Carbon films were deposited on PLLA foils from distances of 3 to 7 cm. Subsequent heat-treatment (60 °C, 1 h) created lamellar structures with dimensions decreasing from micro- to nanoscale with increasing deposition distance. All carbon films improved the spreading and proliferation of human osteoblast-like MG 63 cells, and promoted the alignment of these cells along the lamellar structures. Similar alignment was observed in human osteoblast-like Saos-2 cells and in human dermal fibroblasts. Type I collagen fibers produced by Saos-2 cells and fibroblasts were also oriented along the lamellar structures. These structures increased the activity of alkaline phosphatase in Saos-2 cells. Carbon coatings also supported adhesion and growth of vascular endothelial and smooth muscle cells, particularly flatter non-heated carbon films. On these films, the continuity of the endothelial cell layer was better than on heat-treated lamellar surfaces. Heat-treated carbon-coated PLLA is therefore more suitable for bone and skin tissue engineering, while carbon-coated PLLA without heating is more appropriate for vascular tissue engineering.

• MeSH
• biokompatibilní potahované materiály chemie   farmakologie   MeSH
• buněčná adheze účinky léků   MeSH
• buněčná diferenciace účinky léků   MeSH
• buněčné linie MeSH
• kolagen typu I metabolismus   MeSH
• lidé MeSH
• polyestery chemie   MeSH
• povrchové vlastnosti MeSH
• testování materiálů MeSH
• tkáňové inženýrství * MeSH
• uhlík chemie   MeSH
• vysoká teplota MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• chemie MeSH
• koloidy MeSH
• Publikační typ
• periodika MeSH

• Konspekt
• Chemie. Mineralogické vědy
• NLK Obory
• chemie, klinická chemie

• MeSH
• biomedicínské inženýrství MeSH
• Publikační typ
• abstrakty MeSH
• kongresy MeSH
• sborníky MeSH

• Konspekt
• Lékařské vědy. Lékařství
• NLK Obory
• biomedicínské inženýrství

In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO2-i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO2-i-PCL due to collagen coating and incorporation of TiO2, respectively. EDX and XPS studies revealed distribution of entrapped TiO2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

• MeSH
• biokompatibilní materiály chemie   MeSH
• buněčná adheze fyziologie   MeSH
• fibroblasty chemie   MeSH
• fotoelektronová spektroskopie metody   MeSH
• hydrofobní a hydrofilní interakce MeSH
• kolagen chemie   MeSH
• kůže chemie   MeSH
• nanovlákna chemie   MeSH
• polyestery chemie   MeSH
• povrchové vlastnosti MeSH
• titan chemie   MeSH
• tkáňové inženýrství metody   MeSH
• tkáňové podpůrné struktury MeSH
• Publikační typ
• časopisecké články MeSH

In this study, fibrous scaffolds based on poly(γ-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20-400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres' surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N5 -hydroxyethyl-l-glutamine units; and second, modification with the short peptide sequence azidopentanoyl-GGGRGDSGGGY-NH2 , using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the 125 I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10-3 -10 pm/cm2 ). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm2 and 200 ± 100 fm/cm2 RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs. Copyright © 2015 John Wiley & Sons, Ltd.

• MeSH
• benzylové sloučeniny chemická syntéza   chemie   farmakologie   MeSH
• chrupavka účinky léků   fyziologie   MeSH
• click chemie MeSH
• dítě MeSH
• glutamáty chemická syntéza   chemie   farmakologie   MeSH
• glykosaminoglykany metabolismus   MeSH
• kmenové buňky cytologie   účinky léků   MeSH
• kultivované buňky MeSH
• lidé MeSH
• magnetická rezonanční spektroskopie MeSH
• mladiství MeSH
• peptidy farmakologie   MeSH
• povrchové vlastnosti MeSH
• proliferace buněk účinky léků   MeSH
• tkáňové inženýrství metody   MeSH
• tkáňové podpůrné struktury chemie   MeSH
• viabilita buněk účinky léků   MeSH
• zubní zárodek cytologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Polysaccharides meet several criteria for a suitable biomaterial for tissue engineering, which include biocompatibility and ability to support the delivery and growth of cells. Nevertheless, most of these polysaccharides, for example dextran, alginate, and glycosaminoglycans, are highly soluble in aqueous solutions. Hyaluronic acid hydrophobized by palmitic acid and processed to the form of wet-spun fibers and the warp-knitted textile scaffold is water non-soluble, but biodegradable material, which could be used for the tissue engineering purpose. However, its surface quality does not allow cell attachment. To enhance the biocompatibility the surface of palmitoyl-hyaluronan was roughened by freeze drying and treated by different cell adhesive proteins (fibronectin, fibrinogen, laminin, methacrylated gelatin and collagen IV). Except for collagen IV, these proteins covered the fibers uniformly for an extended period of time and supported the adhesion and cultivation of dermal fibroblasts and mesenchymal stem cells. Interestingly, adipose stem cells cultivated on the fibronectin-modified scaffold secreted increasing amount of HGF, SDF-1, and VEGF, three key growth factors involved in cardiac regeneration. These results suggested that palmitoyl-hyaluronan scaffold may be a promising material for various applications in tissue regeneration, including cardiac tissue repair. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1488-1499, 2018.

• MeSH
• biokompatibilní materiály chemie   MeSH
• buněčná adheze MeSH
• buněčné linie MeSH
• fibronektiny chemie   MeSH
• kmenové buňky cytologie   MeSH
• kultivované buňky MeSH
• kyselina hyaluronová chemie   MeSH
• kyselina palmitová chemie   MeSH
• lidé MeSH
• povrchové vlastnosti MeSH
• proliferace buněk MeSH
• tkáňové inženýrství MeSH
• tkáňové podpůrné struktury chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• biomedicínské a zubní materiály MeSH
• chemické inženýrství MeSH
• glukosaoxidasa MeSH
• metody MeSH
• povrchové vlastnosti MeSH
• transplantace orgánů přístrojové vybavení   MeSH
• transplantační imunologie MeSH
• umělé srdce MeSH

Pojednání se zabývá seznámením s transmisní profilometrií, která je založena na absorpci rovnoběžného světla po průchodu otiskem kůže. Ten je zhotoven speciálním postupem do standardizované formy modře zbarveným silikonem. Světlo se detekuje CCD kamerou a získané parametry se vyhodnotí k tomu určeným softwarem. Výstupem jsou křivky a číselné hodnoty, které hodnotí nerovnosti, včetně poskytnutí dvojči trojrozměrných obrazů. Metoda umožňuje v přijatelných mezích vyhodnocovat statisticky kvantitativní i kvalitativní změny kožního povrchu a tak objektivizovat nejrůznější fyziologické či patologické jevy nebo léčebné postupy.

Article introduces the method of transmission profilometry based on the absorption of parallel light after penetration of skin imprint. The imprint is made by a special procedure from a blue silicone to a standardized form. The light is detected by a CCD camera and the obtained values are evaluated by a specific software. The output curves and numbers which evaluate the roughness also provide two- or three-dimensional pictures. The method tolerably enables to statistically evaluate quantitative and qualitative changes of skin surface and thus objectively assess various physiological or pathological phenomena or treatment procedures.

• MeSH
• fotometrie metody   přístrojové vybavení   využití   MeSH
• kůže MeSH
• lidé MeSH
• povrch těla MeSH
• software MeSH
• Check Tag
• lidé MeSH