human pulp Dotaz Zobrazit nápovědu
- MeSH
- lidé MeSH
- mikroskopie MeSH
- ortodontické aparáty MeSH
- pevnost v tahu MeSH
- zubní dřeň anatomie a histologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- hydroxid vápenatý chemie terapeutické užití MeSH
- lidé MeSH
- nemoci zubní dřeně klasifikace terapie MeSH
- odhalení zubní dřeně terapie MeSH
- překrytí zubní dřeně metody trendy využití MeSH
- terapie kořenového kanálku metody trendy využití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- extrakce zubů MeSH
- lidé MeSH
- replantace MeSH
- zubní dřeň enzymologie patologie MeSH
- Check Tag
- lidé MeSH
BACKGROUND AND AIMS: Teeth extracted are usually disposed as bio-waste whereas they could serve as an autologous tissue for culturing multipotent dental pulp cells which have application potential in regenerative medicine. This study aimed to examine the feasibility of cryopreserving dental pulp tissue at teeth extraction for later culturing of cells. METHODS: The pulp tissue from each of a total of 10 teeth was cut into small fragments which were then divided into two portions. One portion was directly used for culturing pulp cells using the explant method. The other portion was cryopreserved with 10% DMSO in liquid nitrogen for at least one month and then thawed for culturing pulp cells. RESULTS: Vital cells were obtained from all the 10 pulp fragment suspensions which went through cryopreservation. The cell outgrowth from the explants of cryopreserved pulp fragments was two days later than that of corresponding fresh pulp tissue. Otherwise, no difference was observed in proliferation, expression of stem cell markers and differentiation into adipose cells and osteoblasts between the two groups of cells cultured from the fresh or the cryopreserved pulp fragments. CONCLUSIONS: Cryopreserving fragmented dental pulp tissue provides a feasible option for saving pulp tissues as autologous cell sources for possible later application.
- MeSH
- buněčná diferenciace MeSH
- buněčné kultury MeSH
- kmenové buňky * MeSH
- kryoprezervace MeSH
- kultivované buňky MeSH
- lidé MeSH
- proliferace buněk MeSH
- zubní dřeň * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
INTRODUCTION: The unique pulp-dentin complex demonstrates strong regenerative potential, which enables it to respond to disease and traumatic injury. Identifying the proteins of the pulp-dentin complex is crucial to understanding the mechanisms of regeneration, tissue calcification, defense processes, and the reparation of dentin by dental pulp. The lack of knowledge of these proteins limits the development of more efficient therapies. METHODS: The proteomic profile of human tooth pulp was investigated and compared with the proteome of human dentin and blood. The samples of tooth pulp were obtained from 5 sound permanent human third molars of 5 adults (n = 5). The extracted proteins were separated by 2-dimensional gel electrophoresis, analyzed by nano-liquid chromatography tandem mass spectrometry, and identified by correlating mass spectra to the proteomic databases. RESULTS: A total of 342 proteins were identified with high confidence, and 2 proteins were detected for the first time in an actual human sample. The identified tooth pulp proteins have a variety of functions: structural, catalytic, transporter, protease activity, immune response, and many others. In a comparison with dentin and blood plasma, 140 (pulp/dentin) shared proteins were identified, 37 of which were not observed in plasma. It can be suggested that they might participate in the unique pulp-dentin complex. CONCLUSIONS: This proteomic investigation of human tooth pulp, together with the previously published study of human dentin, is one of the most comprehensive proteome lists of human teeth to date.
- MeSH
- 2D gelová elektroforéza MeSH
- chromatografie kapalinová MeSH
- dentin chemie MeSH
- dospělí MeSH
- energetický metabolismus fyziologie MeSH
- hmotnostní spektrometrie MeSH
- imunoproteiny analýza MeSH
- krevní proteiny analýza MeSH
- lidé MeSH
- mezibuněčná komunikace fyziologie MeSH
- mladý dospělý MeSH
- nanotechnologie MeSH
- proliferace buněk MeSH
- proteiny metabolismus MeSH
- proteom analýza klasifikace MeSH
- signální transdukce fyziologie MeSH
- tandemová hmotnostní spektrometrie MeSH
- zubní dřeň chemie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- MeSH
- elektronová mikroskopie MeSH
- lidé MeSH
- slezina krevní zásobení ultrastruktura MeSH
- Check Tag
- lidé MeSH
