quantitative image analysis
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Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.
Práce hodnotí subjektivní názor dětí staršího školního věku na svůj vzhled a postavu, zda jsou spokojeni s tím jak vypadají a kolik váží, nebo zda touží po hubenější resp. svalnatější postavě. Dále zjistit zda s touhou po hubenější nebo svalnatější postavě mění stravovací návyky jedí více zeleniny, méně sladkostí nebo vynechávají některá denní jídla a drží diety. K tomuto zjištění byl použit dotazník, jehož součástí byl také test přiřazování siluet. Výsledky studie zahrnující 109 chlapců a 112 dívek ukázaly, že chlapci jsou mnohem více spokojeni se svým vzhledem a hmotností než dívky. 70 % chlapců je spokojeno se svou váhou, naproti tomu 57 % dívek si přeje vážit méně a vypadat štíhlejší. Dále bylo zjištěno, že dívky častěji než chlapci vynechávají některá denní jídla nebo drží diety. Z celkového souboru mělo 32 % respondentů nadváhu nebo obezitu.
The goal of this study was to measure the subjective body image and weight satisfaction of young ages 11–15. Satisfaction with their body image was measured by a series of questionnaires and other interactive interview techniques. From these results, a plan of suggested dietary changes, such as increasing daily servings of fruits and vegetables or in some cases omitting meals to reduce their overall calorie intake. An important part of the study was the silhouette matching section. The results of the study of 109 boys and 112 girls confi rmed that boys are more satisfi ed with their body than girls. In general, 70 % of boys were more satisfi ed with their bodies than girls. Many girls (57 %) felt that they should be slimmer and were actively dieting to reduce their body weight. Even though the diet regimen was a common behaviour in the girls, 32% of all respondents were overweigh or obese.
- Klíčová slova
- body image, mentální bulimie, bigorexie, objektivní a subjektivní hodnocení tělesných proporcí, test přiřazování siluet,
- MeSH
- bulimia nervosa prevence a kontrola MeSH
- dieta MeSH
- hodnotící studie jako téma MeSH
- lidé MeSH
- mentální anorexie prevence a kontrola MeSH
- mladiství * MeSH
- osobnost MeSH
- poruchy příjmu potravy prevence a kontrola MeSH
- sebepojetí * MeSH
- školy MeSH
- statistika jako téma MeSH
- stravovací zvyklosti psychologie MeSH
- tělesná hmotnost MeSH
- Check Tag
- lidé MeSH
- mladiství * MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
Jedním z pilířů, které výrazně utváří sebepojetí již od dětství, je fyzický vzhled. K měření spokojenosti s vlastním vzhledem se často využívají obrázkové škály postav. Hlavním cílem bylo vytvoření a ověření obrázkové škály postav nesoucí název Basic Olomouc Body Rating (BOBR). Škála byla vytvořena metodou analýzy dokumentů týkající se body-rating škál a metodou rozhovoru s potenciálními probandy. Výsledkem je vytvoření škály postav využitelné v dalším výzkumu i poradenské praxi. Text seznamuje stručně i s výsledky následné studie – internetový výzkum týkající se body image a vnímání sexuální atraktivity. n=5616 respondentů z České republiky odpovídalo na otázky, jak vypadají a jak by chtěli vypadat. Výsledky ukázaly signifikantní rozdíl jak u mužů, tak u žen (p < 0,0001; p < 0,0001). Výsledkem je vytvoření škály BOBR, která je validována a připravena k použití pro další výzkumy.
Problem and methods: Physical appearance is one of the key components of selfperception from early childhood. An individual's perceived physical attractiveness is largely conditioned by geographical, cultural and historical factors. Every culture develops its own criteria of attractiveness and any deviations to those are often a cause of ostracism, for example through exclusion or rejection. Still, there are certain universal principles of attractiveness (e.g. a higher waist-hip ratio, facial symmetry, sexually dimorphic features), which exist across cultures and time periods with little variation. To measure a person's level of satisfaction with his/her physical appearance, psychologists regularly employ figure rating scales. The primary goal of our work was to develop and verify an updated visual body rating scale called the Basic Olomouc Body Rating (BOBR), making sure that it is widely usable, valid and reliable. The scale was created using the method of document analysis of academic papers according body-rating scales and a method of interview with potential probands. In the pilot data gathering phase, a group of respondents was presented with the 3 scales commonly used in the European context, i.e. FDS (Stunkard, Sorensen & Schulsinger, 1983), CDRS (Thompson & Gray, 1995) and BIAS-BD (Gardner, Jappe & Gardner, 2009). The purpose of this was to get feedback on these scales and find out if people are able to use these scales to rate themselves. New schematic figure rating scale for both men and women which would do away with the limitations of the scales used so far was developed. Results: The result is creation of a body-rating scale widely usable in further research and practical consulting. The paper briefly summarizes results of an additional study – the goal definition phase was followed by online research on the subject of body image and the self-perceived sexual attractiveness. Altogether, 5,616 respondents from the Czech Republic responded to questions such as ‘How do I look?', ‘How do I want to look?' and ‘Which figures of the same and opposite sex do I find the most sexually attractive?' The participants indicated their responses on the BOBR 10-point figure rating scale. The results showed a significant divergence between the responses to the questions ‘I want to look' and ‘I look' among men, but also among women (p = 0.00; p = 0.00). Our research findings do not differ from the findings of other authors. Body dissatisfaction is a relatively common phenomenon, especially in women and in relation to weight. While women would like to be thinner, body dissatisfaction in men goes both ways, i.e. men, more frequently than women, would like to have a fuller figure. Compared with women, men are more frequently dissatisfied with their height. Conclusion: The main conclusion of the study is creation of the BOBR figure – rating scale, which was validated and is ready to be used in further researches. Further on, the scale has been verified in terms of validity and reliability; and a significant divergence was found between the responses to the questions ‘I want to look' and ‘I look' among both men and women. At this stage, the scale has been validated as an instrument and demonstrated to be useful for a variety of tasks: The fact that the scale has been received well also increases its potential and usability, for example in consulting psychology or as an ice-breaker during therapy sessions for individuals suffering from eating disorders.
- MeSH
- dospělí MeSH
- hodnotící studie jako téma MeSH
- internet MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- muži psychologie MeSH
- osobnostní testy * MeSH
- představa o vlastním těle * psychologie MeSH
- reprodukovatelnost výsledků MeSH
- sebepojetí MeSH
- výzkumný projekt MeSH
- ženy psychologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
BACKGROUND: Optical coherence tomography (OCT) is widely used in ophthalmology for viewing the morphology of the retina, which is important for disease detection and assessing therapeutic effect. The diagnosis of retinal diseases is based primarily on the subjective analysis of OCT images by trained ophthalmologists. This paper describes an OCT images automatic analysis method for computer-aided disease diagnosis and it is a critical part of the eye fundus diagnosis. METHODS: This study analyzed 300 OCT images acquired by Optovue Avanti RTVue XR (Optovue Corp., Fremont, CA). Firstly, the normal retinal reference model based on retinal boundaries was presented. Subsequently, two kinds of quantitative methods based on geometric features and morphological features were proposed. This paper put forward a retinal abnormal grading decision-making method which was used in actual analysis and evaluation of multiple OCT images. RESULTS: This paper showed detailed analysis process by four retinal OCT images with different abnormal degrees. The final grading results verified that the analysis method can distinguish abnormal severity and lesion regions. This paper presented the simulation of the 150 test images, where the results of analysis of retinal status showed that the sensitivity was 0.94 and specificity was 0.92.The proposed method can speed up diagnostic process and objectively evaluate the retinal status. CONCLUSIONS: This paper aims on studies of retinal status automatic analysis method based on feature extraction and quantitative grading in OCT images. The proposed method can obtain the parameters and the features that are associated with retinal morphology. Quantitative analysis and evaluation of these features are combined with reference model which can realize the target image abnormal judgment and provide a reference for disease diagnosis.
- MeSH
- diagnóza počítačová MeSH
- dospělí MeSH
- fundus oculi MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- optická koherentní tomografie * MeSH
- počítačové zpracování obrazu * MeSH
- poměr signál - šum MeSH
- retina diagnostické zobrazování MeSH
- rozhodování MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
The endoneurial extracellular matrix (ECM) molecules are involved in cell signalling during nervous system development and regeneration. Quantitative differences of immunofluorescence labelling for chondroitin sulfate proteoglycan (CSPG), fibronectin (FN), tenascin-C (TN-C), and thrombospondin (TSP) were evaluated in intact rat dorsal and ventral roots and dorsal and ventral roots 2 and 4 weeks after rhizotomy using image analysis. The distal stumps of spinal roots displayed increased immunolabelling for the molecules with higher immunofluorescence in dorsal than in ventral roots up to 2 weeks from transection. Four weeks after rhizotomy, the immunoreactivity for CSPG, TN-C and TSP decreased in dorsal and increased in ventral root stumps, although a higher level of immunofluorescence for FN remained in both dorsal and ventral root stumps 4 weeks after injury in comparison to 2 weeks after injury. We suggest that the amount of some ECM molecules changed differentially 2 and 4 weeks after rhizotomy to create an appropriate environment in the endoneurium for early and later regrowth of sensory and motor axons. The results presented here are the first report of differences between the endoneurial ECM content of damaged afferent and motor nerve fibers. In addition, the immunohistochemical detection of individual ECM molecules indicated that final extrinsic conditions stimulating the regrowth of regenerating axons probably arise from a balance of both growth-promoting and -inhibiting molecules in the endoneurium.
- MeSH
- chondroitinsulfát proteoglykany analýza MeSH
- fibronektiny analýza MeSH
- financování organizované MeSH
- fluorescenční protilátková technika nepřímá MeSH
- imunohistochemie MeSH
- kryoultramikrotomie MeSH
- krysa rodu rattus MeSH
- míšní kořeny chemie patologie růst a vývoj zranění MeSH
- motorické neurony patologie MeSH
- neurony aferentní patologie MeSH
- periferní nervy chemie patologie ultrastruktura MeSH
- počítačové zpracování obrazu metody MeSH
- potkani Wistar MeSH
- regenerace nervu MeSH
- rizotomie MeSH
- tenascin analýza MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- autoimunitní tyreoiditida farmakoterapie ultrasonografie MeSH
- dospělí MeSH
- finanční podpora výzkumu jako téma MeSH
- index tělesné hmotnosti MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- počítačové zpracování obrazu MeSH
- protilátky krev MeSH
- regresní analýza MeSH
- senioři MeSH
- thyreotropin krev MeSH
- thyroxin terapeutické užití MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
