Pojmem vrozené koagulopatie označujeme krvácivé stavy zapříčiněné sníženou hladinou anebo poruchou funkce jednoho nebo více koagulačních faktorů. Hlavním klinickým projevem poklesu hladiny koagulačních faktorů je zvýšený sklon ke krvácení, ale v některých případech je to zcela opačně a hluboké deficity koagulačních faktorů doprovází zvýšený sklon k trombotizaci (FXII a ostatní faktory kontaktní fáze, část dysfibrinogenémií).
“Congenital coagulopathies” is a term denoting haemorrhaging conditions caused by low levels of functional disorder involving one or more coagulation factors. The main clinical symptom of low levels of CF is increased tendency to haemorrhage, but in some cases the opposite applies and deep deficits of coagulation factors can be accompanied by tendency to form thrombi (FXII and other factors of the contact phase, some dysfibrinogenemias).
- MeSH
- Afibrinogenemia diagnosis etiology drug therapy therapy MeSH
- Antifibrinolytic Agents therapeutic use MeSH
- Blood Coagulation Disorders, Inherited diagnosis therapy MeSH
- Deamino Arginine Vasopressin therapeutic use MeSH
- Factor IX therapeutic use MeSH
- Factor VIII therapeutic use MeSH
- Hemophilia A * diagnosis therapy MeSH
- Hemophilia B * diagnosis therapy MeSH
- Kininogen, High-Molecular-Weight deficiency MeSH
- Blood Coagulation Factors therapeutic use MeSH
- Blood Coagulation Disorders * diagnosis therapy MeSH
- Hemorrhage prevention & control therapy MeSH
- Humans MeSH
- Factor XI Deficiency diagnosis drug therapy therapy MeSH
- Factor XII Deficiency diagnosis MeSH
- Factor XIII Deficiency diagnosis therapy MeSH
- Prekallikrein deficiency MeSH
- von Willebrand Diseases diagnosis drug therapy therapy MeSH
- Rare Diseases diagnosis therapy MeSH
- Check Tag
- Humans MeSH
Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication and potentially life-threatening condition resulting from excessive ovarian stimulation during assisted reproductive technologies. Our aim was to identify candidate proteins in follicular fluid (FF) using various proteomic approaches which may help to identify patients at risk of OHSS. We analysed the proteome alterations in FF from patients suffering from severe forms of OHSS (OHSS+) compared with a control group of women without or with only mild signs of OHSS (OHSS-). The 12 abundant proteins of FF were removed using an immunoaffinity system. Pools of remaining depleted proteins were applied to the two-dimensional (2D) electrophoresis and 2D liquid chromatography and proteins in differentially expressed protein spots/fractions were identified by mass spectrometry. Among a total of 19 candidate proteins differentially expressed (P< 0.05) between OHSS+ and OHSS- FF samples, three proteins, namely ceruloplasmin, complement C3 and kininogen-1, were found using both 2D techniques. Computer modelling highlighted the important role of kininogen-1 as an anchor for mediated interactions with other identified proteins including ferritin light chain and ceruloplasmin, hepatocyte growth factor-like protein, as well as complement C3 and gelsolin, thus linking various biological processes including inflammation and angiogenesis, iron transport and storage, blood coagulation, innate immunity, cell adhesion and actin filament polymerization. The delineation of such processes may allow the development of informed corrective therapeutic intervention in patients at risk of OHSS and a set of key proteins of the FF may be helpful as potential biomarkers for monitoring IVF therapy.
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Fertilization in Vitro adverse effects MeSH
- Follicular Fluid chemistry metabolism MeSH
- Immunoblotting MeSH
- Kininogens chemistry metabolism MeSH
- Humans MeSH
- Ovarian Hyperstimulation Syndrome etiology MeSH
- Computer Simulation MeSH
- Proteomics MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Acta medica Scandinavica, ISSN 0365-463X suppl. 620
62 s. : grafy ; 24 cm
