In an increasingly aging society, there is a growing demand for the development of technology related to tissue regeneration. It involves the development of the appropriate biomaterials whose properties will allow the desired biological response to be obtained. Bioactivity is strongly affected by the proper selection of active ingredients. The aim of this study was to produce bioactive hydrogel materials based on hyaluronic acid and collagen modified by the addition of placenta. These materials were intended for use as dressings, and their physicochemical properties were investigated under simulated biological environmental conditions. The materials were incubated in vitro in different fluids simulating the environment of the human body (e.g., simulated body fluid) and then stored at a temperature close to body temperature. Using an FT-IR spectrophotometer, the functional groups present in the composites were identified. The materials with the added placenta showed an increase in the swelling factor of more than 300%. The results obtained confirmed the potential of using this material as an absorbent dressing. This was indicated by pH and conductometric measurements, sorption, degradation, and surface analysis under an optical microscope. The results of the in vitro biological evaluation confirmed the cytosafety of the tested biomaterials. The tested composites activate monocytes, which may indicate their beneficial properties in the first phases of wound healing. The material proved to be nontoxic and has potential for medical use.
- MeSH
- biokompatibilní materiály farmakologie MeSH
- hojení ran MeSH
- hydrogely * farmakologie chemie MeSH
- kolagen farmakologie chemie MeSH
- kyselina hyaluronová * farmakologie chemie MeSH
- lidé MeSH
- ovce MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
This study reports on the successful use of a machine learning approach using attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectroscopy for the classification and prediction of a donor's sex from the fingernails of 63 individuals. A significant advantage of ATR FT-IR is its ability to provide a specific spectral signature for different samples based on their biochemical composition. The infrared spectrum reveals unique vibrational features of a sample based on the different absorption frequencies of the individual functional groups. This technique is fast, simple, non-destructive, and requires only small quantities of measured material with minimal-to-no sample preparation. However, advanced multivariate techniques are needed to elucidate multiplex spectral information and the small differences caused by donor characteristics. We developed an analytical method using ATR FT-IR spectroscopy advanced with machine learning (ML) based on 63 donors' fingernails (37 males, 26 females). The PLS-DA and ANN models were established, and their generalization abilities were compared. Here, the PLS scores from the PLS-DA model were used for an artificial neural network (ANN) to create a classification model. The proposed ANN model showed a greater potential for predictions, and it was validated against an independent dataset, which resulted in 92% correctly classified spectra. The results of the study are quite impressive, with 100% accuracy achieved in correctly classifying donors as either male or female at the donor level. Here, we underscore the potential of ML algorithms to leverage the selectivity of ATR FT-IR spectroscopy and produce predictions along with information about the level of certainty in a scientifically defensible manner. This proof-of-concept study demonstrates the value of ATR FT-IR spectroscopy as a forensic tool to discriminate between male and female donors, which is significant for forensic applications.
In the present study, a single Arcanobacterium (A.) pinnipediorum strain isolated from discharge of a jaw swelling of a grey seal pup (Halichoerus grypus) in England, UK, was identified. This strain was further characterized by phenotypical investigations, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), by Fourier transform infrared spectroscopy (FT-IR), and genotypically by sequencing the 16S rRNA gene and the genes gap encoding glyceraldehyde 3-phosphate dehydrogenase, tuf encoding elongation factor tu, and rpoB encoding the β subunit of bacterial RNA polymerase. The present study gives a first detailed characterization of the species A. pinnipediorum from a grey seal in the UK. However, the route of infection of the grey seal with the bacterial pathogen remains unclear.
- MeSH
- Arcanobacterium * genetika MeSH
- RNA ribozomální 16S genetika MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- tuleňovití * genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Spojené království MeSH
Efficient bacterial strain was isolated from the dye contaminated area and identified as Bacillus stratosphericus SCA1007 based on 16S rRNA gene sequence (GenBank under accession number KY992944). This isolate was selected based on its potential to efficiently decolorize reactive orange 16 dye which is extensively used in textile industries. Various culture conditions like dye concentration, temperature, pH, salinity, and additional nitrogen source were optimized in the present study. The optimal conditions for decolorization of reactive orange 16 was found to be: dye concentration 150 mg/L, pH 7, temperature 35 °C, and yeast extract as nitrogen source. The isolate was also resistant to 4% saline culture condition. Decolorization and degradation of dye were confirmed through UV-visible spectroscopy, Fourier transform infrared (FTIR) and liquid chromatography-mass spectrometry analysis (LC-MS). Toxicity studies were performed on Escherichia coli and Vigna radiata to confirm the non-toxic nature of the degraded metabolites. This is the first study demonstrating complete decolorization of reactive orange 16 dye by Bacillus stratosphericus SCA1007 at high salinity within 10 h of incubation under optimized conditions.
Wound dressing materials fabricated using biocompatible polymers have become quite relevant in medical applications, and one such material is bacterial cellulose (BC) with exceptional properties in terms of biocompatibility, high purity, crystallinity (∼88%), and high water holding capacity. However, the lack of antibacterial activity slightly restricts its application as a wound dressing material. In this work, polycaprolactone (PCL) was first impregnated into the BC matrix to fabricate flexible bacterial cellulose-based PCL membranes (BCP), which was further functionalized with antibiotics gentamicin (GEN) and streptomycin (SM) separately, to form wound dressing composite scaffolds to aid infectious wound healing. Fourier transform infrared spectroscopy (FT-IR) results confirmed the presence of characteristic PCL and cellulose peaks in the composite scaffolds at 1720 cm-1, 3400 cm-1, and 2895 cm-1, respectively, explaining the successful interaction of PCL with the BC matrix, which is further corroborated by scanning electron microscopy (SEM) images. X-ray diffraction (XRD) studies revealed the formation of highly crystalline BCP films (∼86%). In vitro studies of the BC and BCP scaffolds against baby hamster kidney (BHK-21) cells revealed their cytocompatible nature; also the wettability studies indicated the hydrophilicity of the developed scaffolds, qualifying the main criterion in wound dressing applications. Energy dispersive X-ray analysis (EDX) of the drug loaded scaffolds showed the presence of sulfur in the composites. The prepared scaffolds also exhibited excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus. The release profiles initially indicated a burst release (6 h) followed by controlled release of GEN (∼42%) and SM (∼58%) from the prepared scaffolds within 48 h. Hence, these results interpret that the prepared drug-functionalized cellulosic scaffolds have great potential as a wound dressing material in biomedical applications.
- MeSH
- antibakteriální látky farmakologie MeSH
- Bacteria MeSH
- celulosa * farmakologie MeSH
- Escherichia coli MeSH
- hojení ran MeSH
- mikrobiální testy citlivosti MeSH
- obvazy * MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This study illustrates the synthesis of functionalized carbon quantum dots (CQDs) by the one-pot pyrolysis method. The functionalization agent used in CQD synthesis was poly l- lysine (PLL). Various physicochemical techniques were employed to confirm the successful formation of PLLCQD including High resolution transmission electron microscopy (HR-TEM), UV-Vis spectroscopy, fluorescence spectroscopy; Atomic force microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The size of PLLCQD was confirmed by HRTEM and AFM. The synthesized PLLCQD shows bright blue fluorescence and has a quantum yield of 19.35%. The highest emission band was observed at 471nm when excited to 370nm. The prepared PLLCQD exhibited excellent antibacterial activity against Escherichia coli and Staphylococcus aureus with inhibition zone 7-20 mm. The concentrations of 0.9 to 0.1gmL-1 were studied to determine minimum inhibitory concentration (MIC) by the agar well diffusion assay method. MIC of 0.2gml -1 concentration of PLLCQD is achieved. The anti-angiogenic activity of PLLCQD was determined using (Chick Chorioallantoic Membrane) CAM assay. CAM assay is a reliable in -vivo model to study angiogenesis also; many stimulators and inhibitors have been examined by this method. This study proves higher antibacterial efficiency of PLLCQD over non functionalized CQD. PLLCQD was successfully employed in bio-imaging of the bacterial cell through fluorescence microscopy. Further, PLLCQD displayed cytotoxic effect on endothelial cells and inhibited blood vessel formation in the CAM model.
Zein is renewable plant protein with valuable film-forming properties that can be used as a packaging material. It is known that the addition of natural cross-linkers can enhance a film's tensile properties. In this study, we aimed to prepare antimicrobial zein-based films enriched with monolaurin, eugenol, oregano, and thyme essential oil. Films were prepared using the solvent casting technique from ethanol solution. Their physicochemical properties were investigated using structural, morphological, and thermal techniques. Polar and dispersive components were analyzed using two models to evaluate the effects on the surface free energy values. The antimicrobial activity was proven using a disk diffusion method and the suppression of bacterial growth was confirmed via a growth kinetics study with the Gompertz function. The films' morphological characteristics led to systems with uniform distribution of essential oils or eugenol droplets combined with a flat-plated structure of monolaurin. A unique combination of polyphenolic eugenol and amphiphilic monoglyceride provided highly stretchable films with enhanced barrier properties and efficiency against Gram-positive and Gram-negative bacteria, yeasts, and molds. The prepared zein-based films with tunable surface properties represent an alternative to non-renewable resources with a potential application as active packaging materials.
- MeSH
- antibakteriální látky farmakologie MeSH
- antifungální látky farmakologie MeSH
- biomechanika účinky léků MeSH
- diferenciální skenovací kalorimetrie MeSH
- Escherichia coli účinky léků MeSH
- eugenol farmakologie MeSH
- laurany farmakologie MeSH
- mikroskopie atomárních sil MeSH
- monoglyceridy farmakologie MeSH
- obaly potravin * MeSH
- oleje prchavé farmakologie MeSH
- pára MeSH
- permeabilita MeSH
- povrchové vlastnosti MeSH
- smáčivost MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Staphylococcus aureus účinky léků MeSH
- zein farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
In this study, a simple and green strategy was reported to prepare bimetallic nanoparticles (NPs) by the combination of zinc oxide (ZnO) and copper oxide (CuO) using Sambucus nigra L. extract. The physicochemical properties of these NPs such as crystal structure, size, and morphology were studied by X-ray diffraction (XRD), field emission gun scanning electron microscopy (FEG-SEM), and transmission electron microscopy (TEM). The results suggested that these NPs contained polygonal ZnO NPs with hexagonal phase and spherical CuO NPs with monoclinic phase. The anticancer activity of the prepared bimetallic NPs was evaluated against lung and human melanoma cell lines based on MTT assay. As a result, the bimetallic ZnO/CuO NPs exhibited high toxicity on melanoma cancer cells while their toxicity on lung cancer cells was low.
- MeSH
- antibakteriální látky chemie farmakologie MeSH
- bez černý chemie MeSH
- buňky A549 MeSH
- cytotoxiny chemie farmakologie MeSH
- difrakce rentgenového záření metody MeSH
- kovové nanočástice chemie MeSH
- lidé MeSH
- listy rostlin chemie MeSH
- měď chemie farmakologie MeSH
- mikrobiální testy citlivosti metody MeSH
- nádorové buněčné linie MeSH
- oxid zinečnatý chemie farmakologie MeSH
- rostlinné extrakty chemie farmakologie MeSH
- spektroskopie infračervená s Fourierovou transformací metody MeSH
- technologie zelené chemie metody MeSH
- transmisní elektronová mikroskopie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The design of improved biopolymeric based hydrogel materials with high load-capacity to serve as biocompatible drug carriers is a challenging task with vital implications in health sciences. In this work, chitosan crosslinked dialdehyde xanthan gum interpenetrated hydroxypropyl methylcellulose gels were developed for the controlled delivery of different antibiotic drugs including ampicillin, minocycline and rifampicin. The prepared hydrogel scaffolds were characterized by rheology method, FTIR, SEM, TGA and compression analysis. In addition, gelation kinetics, swelling, in vitro degradation and drug release rate were studied under simulated gastrointestinal fluid conditions of pH 2.0 and 7.4 at 37 °C. Results demonstrated the gel composition and structure affected drug release kinetics. The release study showed more than 50% cumulative release within 24 h for all investigated antibiotic drugs. In vitro cell cytocompatibility using mouse embryonic fibroblast cell lines depicted ≥80% cell viability, indicating the gels are non-toxic. Finally, the antibacterial activity of loaded gels was evaluated against Gram-negative and positive bacteria (Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia), which correlated well with swelling and drug release results. Overall, the present study demonstrated that the produced hydrogel scaffolds serves as promising material for controlled antibiotic delivery towards microbial growth inhibition.
- MeSH
- ampicilin farmakologie MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální polysacharidy chemie MeSH
- biokompatibilní materiály chemie MeSH
- buněčné linie MeSH
- chitosan chemie MeSH
- deriváty hypromelózy chemie MeSH
- Escherichia coli účinky léků MeSH
- fibroblasty MeSH
- hydrogely chemická syntéza chemie farmakokinetika toxicita MeSH
- koncentrace vodíkových iontů MeSH
- mikrobiální testy citlivosti MeSH
- mikroskopie elektronová rastrovací MeSH
- minocyklin farmakologie MeSH
- myši MeSH
- nosiče léků chemie MeSH
- reologie MeSH
- rifampin farmakologie MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Staphylococcus aureus účinky léků MeSH
- termogravimetrie MeSH
- uvolňování léčiv MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
