Cíl studie: Vyhodnocením základních spermiologických parametrů podle standardů WHO u potenciálních dárců spermatu přispět k současné diskusi o mužském faktoru neplodnosti. Typ studie: Retrospektivní demografická studie. Název a sídlo pracoviště: Gyn.-por. klinika LF UP a FN Olomouc, Ústav biologie LF UP Olomouc. Metodika: V období leden 2000 – duben 2004 se dostavilo ke vstupnímu vyšetření celkem 108 potenciálních dárců spermií ve věku 18–35 let. Spermiologické vyšetření čerstvého semene získaného masturbací bylo provedeno podle standardů WHO. Vyhodnocovanými parametry byly objem ejakulátu, koncentrace spermií a celková motilita. Výsledky: Normospermie byla zjištěna jen u 46 mužů (42,6 %). U tří mužů (2,8 %) byla prokázána azoospermie. Nejčastějším patologickým nálezem byla astenospermie, která byla zjištěna u 49 (46,6 %) mužů. Závěr: Na základě rozboru získaných dat můžeme usuzovat na zřejmý pokles kvality spermiologických parametrů mladé české populace. Podle našeho názoru by provedení multicentrické analýzy se zaměřením na hodnocení plodnosti českých mužů bylo vysoce žádoucí.

Objective: To contribute to the contemporary discussion on the male factor infertility through the evaluation of the basic spermatological parameters in men recruiting for semen donation. Design: A retrospective demographic study. Setting: Dept. of Obstetrics and Gynaecology, Dept. of Biology, Faculty of Medicine, Palacký University, Olomouc. Methods: A total of 108 men aged 18 to 35 years came to the preliminary examination for the potential semen donors between January 2000 and April 2004. Spermatological analysis of the fresh ejaculates obtained by masturbation was performed according to WHO guidelines. The following parameters were evaluated: the volume of ejaculate, sperm concentration and total percent motility. Results: Normospermia was found in only 46 men (42.6%). Three males (2.8%) were azoospermic. The most frequent problem was asthenospermia, which was documented in 49 (46.6%) men. Conclusion: The analysis of the obtained data may suggest a marked semen quality decline in the Czech male population. In our opinion, further multicentric epidemiological studies concerning fertility potential in Czech male population seem highly needed.

• MeSH
• dospělí MeSH
• finanční podpora výzkumu jako téma MeSH
• lidé MeSH
• motilita spermií MeSH
• mužská infertilita diagnóza   patologie   MeSH
• počet spermií MeSH
• reprodukční lékařství MeSH
• retrospektivní studie MeSH
• spermabanky MeSH
• žijící dárci MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH

V současné dohě je hledáno vysvětleni pro snižováni parametrů lidského semene pozorovaného v posledních 50 letech. Jednou z možných příčin je kouření. Cílem studie, jejíž dílčí výsledky publikigeme, bylo sledování markerů kvality semene u kouřících a nekouřících mužů a vyhodnocení vlivu multivitaminové a minerálové suplementace. K šetření byla vybrána sociálně homogenní skupina 50 mladých a zdravých studentů vysoké školy vojenské, exponovaná rovněž podobným podmínkám života a profesním faktorům. Vzorky ejakulátu získávané masturbací byly opakovaně vyšetřovány po dobu 2 cyklů spermiogeneze podle standardní metodiky WHO. Dle harmonogramu dvojitého zkříženého slepého pokusu byla podávána multivitaminová a minerálová suplementace vždy 50 % kuřákům a 50 % nekuřákům v kombinaci 8 placebem. Dávky účinných nutrientů doplňovaly jejich přirozený přívod stravou. Objektivním markerem suplementace byla hladina zinku v ejakulátu stanovená metodou AAS. Kvantitativní znaky semene byly ve všech vyšetřeních lepší než limity WHO: objem v průměru 3,45 ml, SD 1,48 ml, hustota spermií 83,5 miUml (SD 63,2), celkový počet spermii 358 mil. (SD 382). Hustota spermií vykazovala statisticky významné sezónní kolísání s maximem v březnu (123 miUml. SD 74,4 miliml). Soubor měl rovněž nadlimitní zastoupení spermií s normálními morfologickými znaky (34,5 % SD 11,6% buněk). Kuřáci měli v průměru signifikantně nižší objem semene než nekuřáci (o 0,13 ml), nižší hustotu spermií (o 4,84 miUml) a nižší celkový počet spermií (o 163 mil.). Základní funkční vlastnost spermií - motilita - byla narušena v celém souboru: v průměru jen 38,3 % (SD 15,7 %) buněk bylo pohyblivých a z nich jen 43,8 % (SD 18,3 %) vykazovalo lineární pohyblivost. Vitaminová a minerálová suplementace signifikantně zlepšila lineární pohyblivost spermií: v ovlivňované skupině se zvýšil počet lineárně pohyblivých buněk o 12,5 % resp. o 17 % ve srovnání se skupinou dostávsgící placebo. Prevalence lineárně pohyblivých buněk signifikantně korelovala s obsaJiem zinku v semeni (regr. koef. r = +0,19, p < 0,01).

The reasons for sperm parameters decrease during the last 50 years are investigated. Smoking can be one of the risk factors. This study observed the markers of human semen quality among smoking and no-smoking men and the influence of multivitamins and minerals supplementation on semen quality. A group of 50 healthy young students of army academy were chosen for their homogenous social, environmental and occupational conditions. Their semen samples were obtained by masturbation repeatedly and investigated according to WHO standardized methods during two cycles of spermiogenesis. 50 % of non-smokers and 50 % of smokers received vitamins and minerals supplementation by the double-blind cross intervention schedule in combination with placebo. The active nutrient doses were calculated as an addition to their natural intake with food. The zinc levels in semen (measured by AAS) were used as a marker of supplementation. Markers of semen quantity were on the average better thant the WHO limits: volume 3.46 (SD 1.48), sperm density 83.5 mil/l ml (SD 63.2), sperm count 358 mil (SD 382 mil). Significant seasonal variation in sperm density has been observed with the highest values in March (123 mil/1 ml, SD 74.4). Also the prevalence of cells with normal morphology was above the WHO limit (34.6 %, SD 11.6 %). Lower average sperm volume (about 0.13 ml), lower sperm density (about 4.84 mil/l ml) and lower sperm count (about 163 mil) were observed among smokers as compared with non-smokers: these differences were significant. Fundamental sperm function - their motUity - has been failured among whole group: on the average, only 38.3 % (SD 16.7 %) of cells were motile and among them, only 43.8 % (SD 18.3 %) were with the linear motility. Supplementation with vitamins and minerals has greatly improved the sperm linear motility: the prevalence of lineary motile cells in the interventioned grou increased about 12.6 % and 17 % resp. in comparison with the placebo group. Th significant linear regression of zinc levels in semen and linear motile cells prevc lence has been documented (r = +0.19, p < 0.01).

• MeSH
• dospělí MeSH
• kouření MeSH
• lidé MeSH
• minerály terapeutické užití   MeSH
• mladiství MeSH
• motilita spermií MeSH
• spermie anatomie a histologie   MeSH
• vitaminy terapeutické užití   MeSH
• výzkum MeSH
• zinek metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• Publikační typ
• přehledy MeSH

Posouzení plodnosti muže je založeno na vyšetření spermatu. Neexistuje univerzální ukazatel, o plodnosti vypovídá až komplex kvantitativních a kvalitativních parametrů. Metody ke stanovení těchto parametrů se stále vyvíjejí a zpřesňují. Jsou standardizovány v Manuálu WHO, který je pravidelně aktualizován. Poslední, šesté vydání je z roku 2021. Cílem přehledového článku je stručně shrnout současné možnosti vyšetření plodnosti muže a současně poukázat na některé změny, které přineslo poslední vydání manuálu.

The assessment of fertility in a man is based on the semen examination. There isn't any single universal indicator, fertility is determined by a complex of quantitative and qualitative parameters. Methods for determining these parameters are evolving and becoming more precise. They are standardised in the WHO Manual, which is regularly updated. The latest is the sixth edition of 2021. The aim of this review article is to summarize the current options for male fertility testing and to highlight some of the changes brought by the latest edition of the manual.

• MeSH
• analýza spermatu * metody   MeSH
• lidé MeSH
• mužská infertilita * diagnóza   MeSH
• směrnice pro lékařskou praxi jako téma normy   MeSH
• sperma cytologie   diagnostické zobrazování   MeSH
• Check Tag
• lidé MeSH

The objective of this study was to investigate the effects of a preparation from herbal extracts (PHE) on libido and semen quality in breeding artificial insemination boars. Ten fertile boars were divided into control and experimental groups according to significant difference of libido. There were no differences in semen quality between groups. Animals were fed a commercial feeding mixture for boars. The feeding mixture for the experimental group was enriched with PHE, which was prepared from Eurycoma longifolia, Tribulus terrestris and Leuzea carthamoides. Duration of the experiment was 10 weeks. Samples of ejaculate were collected weekly. Libido was evaluated according to a scale of 0-5 points. Semen volume, sperm motility, percentage of viable spermatozoa, sperm concentration, morphologically abnormal spermatozoa, daily sperm production and sperm survival were assessed. Amounts of mineral components and free amino acids were analysed in seminal plasma. Significant differences were found in these parameters: libido (4.05 ± 0.22 vs 3.48 ± 0.78; p < 0.001), semen volume (331.75 ± 61.91 vs 263.13 ± 87.17 g; p < 0.001), sperm concentration (386.25 ± 107.95 vs 487.25 ± 165.50 × 10(3) /mm(3); p < 0.01), morphologically abnormal spermatozoa (15.94 ± 11.08 vs 20.88 ± 9.19%; p < 0.001) and Mg concentration (28.36 ± 11.59 vs 20.27 ± 13.93 mm; p < 0.05). The experimental group's libido was increased by 20% in comparison with the beginning of the experiment. Results of this study showed positive effect of PHE on libido and some parameters of boar semen quality.

• MeSH
• analýza spermatu veterinární   MeSH
• krmivo pro zvířata MeSH
• prasata fyziologie   MeSH
• rostlinné přípravky farmakologie   MeSH
• rozvrh dávkování léků MeSH
• sexuální chování zvířat účinky léků   MeSH
• sperma chemie   MeSH
• spermie účinky léků   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky kontrolované MeSH
• práce podpořená grantem MeSH

Examination of semen characteristics is routinely performed for fertility status investigation of the male partner of an infertile couple as well as for evaluation of the sperm donor candidate. A useful tool for preliminary assessment of semen characteristics might be an artificial neural network. Thus, the aim of the present study was to construct an artificial neural network, which could be used for predicting the result of semen analysis based on the basic questionnaire data. On the basis of eleven survey questions two models of artificial neural networks to predict semen parameters were developed. The first model aims to predict the overall performance and profile of semen. The second network was developed to predict the concentration of sperm. The network to evaluate sperm concentration proved to be the most efficient. 92.93% of the patients in the learning process were properly qualified for the group with a correct or incorrect result, while the result for the test set was 85.71%. This study suggests that an artificial neural network based on eleven survey questions might be a valuable tool for preliminary evaluation and prediction of the semen profile.

• MeSH
• analýza spermatu * metody   přístrojové vybavení   MeSH
• lidé MeSH
• motilita spermií MeSH
• mužská infertilita MeSH
• neuronové sítě * MeSH
• počet spermií metody   přístrojové vybavení   MeSH
• průzkumy a dotazníky MeSH
• sperma * MeSH
• spermabanky MeSH
• spermie * abnormality   růst a vývoj   ultrastruktura   MeSH
• Check Tag
• lidé MeSH

Obesity can adversely affect human health, including fertility. While obesity can disturb the hormonal profile of the female organism and is associated with fertility loss, little is known about what effect male obesity has on fertility. The present study analysed sperm samples of 153 donors. The men were selected from couples attending an infertility clinic, who had tried for 12 months or more to achieve pregnancy without success. The age of the men under investigation was recorded, and their body mass index (BMI) was calculated. All semen samples were assessed for volume, concentration, motility and morphology. Sperm chromatin integrity was measured by sperm chromatin structure assay. Quality of sperm chromatin condensation was assessed by toluidine blue, aniline blue and chromomycin A3 staining. We can conclude that the impact of elevated BMI on the parameters investigated (basic semen parameters, chromatin integrity and chromatin condensation) was not proven in this study. On the other hand, ejaculate quality appeared to be affected by ageing. The impact was reflected by chromatin integrity, which is a factor that can substantially affect fertility in men, rather than by basic sperm parameters.

• MeSH
• analýza spermatu MeSH
• chromatin genetika   MeSH
• dospělí MeSH
• fragmentace DNA MeSH
• index tělesné hmotnosti MeSH
• lidé středního věku MeSH
• lidé MeSH
• motilita spermií MeSH
• mužská infertilita etiologie   MeSH
• obezita komplikace   MeSH
• počet spermií MeSH
• sperma MeSH
• spermie cytologie   MeSH
• stárnutí MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: Human papillomavirus (HPV) has been shown to adversely affect human reproduction. We aimed to evaluate the prevalence of human papillomavirus (HPV) infection in men and its correlation with semen parameters and reproductive outcomes. METHODS: Semen samples and penile swabs were collected from potential sperm donors (SD, n = 97) and male partners of infertile couples (IM, n = 328). The presence of HPV DNA in semen samples and penile swabs was analyzed. Associations between hrHPV positive status and fertility outcomes as well as socio-behavioral and health characteristics were evaluated using the R software package. RESULTS: High-risk HPV (hrHPV) genotypes were detected in 28.9% of SD and 35.1% of IM (P = 0.312). Penile swabs were more frequently positive for hrHPV genotypes than semen samples in both IM (32.3% vs. 11.9%, P < 0.001) and SD (26.8% vs. 6.2%, P = 0.006). Men with hrHPV positive semen samples had lower semen volume (median volume 2.5 ml vs. 3 ml, P = 0.009), sperm concentration (median concentration 16 × 106/ml vs. 31 × 106/ml, P = 0.009) and total sperm count (median count 46 × 106 vs. 82 × 106, P = 0.009) than men with hrHPV negative samples. No association was identified between penile hrHPV status and semen parameters. CONCLUSIONS: Our findings indicate that penile HPV infection is common in both potential sperm donors and men from infertile couples. Although HPV positivity is higher in penile swabs, only HPV infection in semen samples affects sperm parameters. However, there was no association between hrHPV positivity in semen and fertility outcomes including abortion rate.

• MeSH
• analýza spermatu MeSH
• charakteristiky rodiny MeSH
• dárci tkání statistika a číselné údaje   MeSH
• dospělí MeSH
• fertilizace in vitro statistika a číselné údaje   MeSH
• infekce papilomavirem komplikace   diagnóza   epidemiologie   MeSH
• infertilita komplikace   diagnóza   epidemiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• Papillomaviridae fyziologie   MeSH
• prognóza MeSH
• sperma fyziologie   virologie   MeSH
• těhotenství MeSH
• výsledek těhotenství epidemiologie   MeSH
• výsledek terapie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

BACKGROUND: We examined sperm quality in a cohort of city policemen in Ostrava at the end of a period with high concentrations of air pollutants (winter) and in the same cohort at the end of a relatively low exposure period (summer). METHODS: The study group was comprised of 54 nonsmoking city policemen living and working in Ostrava, Czech Republic. Average daily air-pollutant concentrations recorded by stationary monitoring for 90 days preceding the collection of semen samples were evaluated for different city districts of Ostrava. Standard semen parameters were assessed according to the guidelines of the World Health Organization (2010). The parameters were semen volume, sperm concentration, sperm morphology, sperm motility, acrosome reaction and sperm plasma membrane integrity. Sperm DNA damage was analysed by the sperm chromatin structure assay (SCSA). Sperm motion characteristics were determined by Computer Assisted Semen Analysis (CASA). RESULTS: The concentrations of all monitored pollutants (particulate matter, sulphur dioxide, nitrogen oxides, carbon monoxide, benzo[a]pyrene, benzene) were significantly increased during winter (p < 0.001), except for ozone, the concentration of which was significantly higher during summer. Sperm volume, concentration, % vitality, % sperm morphology (normal form) and % acrosome-intact sperm did not differ significantly between the monitoring periods. The percentages of total motility and progressive motility were significantly higher in March, i.e. at the end of winter (p = 0.001). However, CASA testing showed differences in sperm motion kinetics between spring and autumn samples. In the spring samples, we found a significantly lower % of straightness (p = 0.044) and the length of straight-line path (p = 0.01), while linearity and straight-line velocity were near the borderline value (p = 0.064; p = 0.054, respectively). As compared to summer, high exposure to air pollution during winter significantly increased the extent of sperm chromatin integrity damage (median 22.6 vs. 18.6%) (p = 0.003) and the proportion of immature spermatozoa (median 11.2 vs. 9.9%, p = 0.001). Sperm DNA damage negatively correlated with total motility and progressive motility (r = -0.611, -0.299; p < 0.001). The negative correlation with vitality, normal morphology and acrosome-intact sperm (r = -0.522, -0.550 and -0.511, respectively) was also significant (p < 0.001). CONCLUSION: The examination of the same cohort of city policemen at the end of a period of high air pollution and at the end of relatively low exposure reduced the effects of age, different lifestyles, different occupational exposures, localities and genetic polymorphism on sperm quality impairment associated with air pollution. This study did not demonstrate impaired standard semen parameters in association with exposure. It was shown that sperm chromatin damage and the percentage of immature sperm were highly sensitive to air pollution.

• MeSH
• analýza spermatu * MeSH
• DNA MeSH
• lidé MeSH
• motilita spermií MeSH
• sperma MeSH
• spermie MeSH
• znečištění ovzduší * statistika a číselné údaje   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Publikační typ
• abstrakt z konference MeSH

The role of asymptomatic infections caused by Ureaplasma species in male infertility and the efficacy of antibiotics in treatment of this failure is not yet definitely determined. A total of 165 infertile males having abnormal semen parameters (study group) as well as 165 healthy fertile men (control group) were included in this study. Semen samples were taken from all participants and after analyzing, undergone real-time PCR, microbial culture, and reactive oxygen species (ROS) as well as total antioxidant capacity (TAC) assays. Infected individuals of study group were treated with antibiotic. One month after the treatment completion, second semen samples were taken and undergone all the tests mentioned. The frequency of Ureaplasma spp. was significantly higher in the infertile men compared with the fertile ones (36.4% versus 11.5%; p < 0.001). Most of semen parameters were improved (p < 0.05) and reached their normal range, the level of TAC elevated (p < 0.001), and ROS level (p = 0.003) as well as ROS/TAC ratio (p = 0.003) reduced after antibiotic treatment. Moreover, wives of 37 infertile men (61.7%) became pregnant six months after the treatment completion. These findings indicate that Ureaplasma species are correlated with male infertility and that antibiotic therapy can improve the semen parameters and treat the male infertility.

• MeSH
• analýza spermatu metody   MeSH
• antibakteriální látky aplikace a dávkování   MeSH
• dospělí MeSH
• lidé MeSH
• motilita spermií účinky léků   MeSH
• mužská infertilita diagnóza   farmakoterapie   mikrobiologie   MeSH
• počet spermií MeSH
• sexuálně přenosné nemoci bakteriální diagnóza   farmakoterapie   MeSH
• ureaplasmatické infekce * diagnóza   farmakoterapie   komplikace   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH