"NT13108" Dotaz Zobrazit nápovědu
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
1 svazek : ilustrace, tabulky ; 30 cm
The project aims to study regulatory cells in patients at the first stage of clinically isolated syndrome (CIS) of multiple sclerosis (MS). Regular assessment of the proportion of different types of regulatory cells, with the emphasis placed on CD8+ cells, should assist in efficient therapy guidance. The first challenge will be to define the subsets that would distinguish patients with CIS from patients with confirmed diagnosis of MS (on the basis of our preliminary results, these might be CD8+CD28-), and from healthy controls. Alterations in the subpopulation might also reflect the possible disease development. Subsequently, the results will be verified by further control groups. The number of regulatory cells will be correlated with the disease development, therapy effects and potential parasite infection. The obtained results will be published in a journal with an impact factor, with sponsor dedication, and used in clinical practice for the selection of therapeutic strategies in patients with CIS.
Projekt je zaměřen na studium regulačních buněk u pacientů ve fázi prvního klinicky izolovaného syndromu (CIS) roztroušené sklerózy (RS), ještě před její definitivní diagnózou. Monitorace zastoupení různých typů regulačních buněk se zaměřením na CD8+ by měla pomoci k účelnému vedení terapie a optimálně zabránit dalšímu vývoji nemoci. V rámci projektu budou opakovaně vyšetřováni pacienti s CIS z RS centra VFN v laboratoři buněčné imunologie ÚIM 1.LF UK. První úkolem bude určit subpopulaci regulačních buněk, která bude odlišovat pacienty s CIS od pacientů s definitivní diagnózou RS (dle předběžných výsledků CD8+CD28-) a zdravých kontrol a která bude odrážet i eventuální vývoj nemoci. Následně pak bude výběr verifikován doplněním kontrolních souborů a hodnoty regulačních buněk budou korelovány s vývojem nemoci a jejím ovlivněním léčbou a možnou parazitární infekcí. Výsledky projektu budou publikovány v časopisech s impact faktorem s dedikací IGA a využity v klinické praxi při léčení nemocných s CIS.
- MeSH
- adherence k farmakoterapii MeSH
- imunofenotypizace MeSH
- interferon beta imunologie terapeutické užití MeSH
- Kaplanův-Meierův odhad MeSH
- lymfocyty cytologie MeSH
- monocyty cytologie MeSH
- prediktivní hodnota testů MeSH
- průtoková cytometrie MeSH
- ROC křivka MeSH
- roztroušená skleróza farmakoterapie MeSH
- software MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- neurologie
- cytologie, klinická cytologie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
BACKGROUND: Clinically isolated syndrome (CIS) represents first neurological symptoms suggestive of demyelinating lesion in the central nervous system (CNS). Currently, there are no sufficient immunological or genetic markers predicting relapse and disability progression, nor there is evidence of the efficacy of registered disease modifying treatments (DMTs), such as intramuscular interferon beta1a. The aim of the study is to evaluate immunological predictors of a relapse or disability progression. METHODS: One hundred and eighty one patients with CIS were treated with interferon beta1a and followed over the period of 4 years. Lymphocyte subsets were analyzed by flow cytometry. A Kaplan-Meier estimator of survival probability was used to analyze prognosis. For statistical assessment only individual differences between baseline values and values at the time of relapse or confirmed disability progression were analysed. RESULTS: Higher levels of B lymphocytes predicted relapse-free status. On the other hand, a decrease of the naïve subset of cells (CD45RA+ in CD4+) after 12, 24, and 36 months of follow-up were associated with an increased risk of confirmed disability progression. CONCLUSION: Our data suggest that the quantification of lymphocyte subsets in patients after the first demyelinating event suggestive of MS may be an important biomarker.
BACKGROUND: Human dirofilariasis is a zoonotic infection that continues to spread to previously unaffected areas of Europe. In the South Moravian Region of the Czech Republic (CR), imported as well as autochthonous canine infections were recorded in the last decade, and parasite DNA was detected in mosquitoes of Aedes vexans. In the present paper, human Dirofilaria infections are reported from the country for the first time. CASE PRESENTATION: The samples from five patients with suspected tissue helminthiases were investigated. In particular cases, nematodes were isolated from various tissues including skin of lower leg, soft tissues of finger, subcutaneous tissue of hypogastrium, lymph node and peritoneum. The diagnosis was based on light microscopic morphology and/or DNA analysis of the worms. In addition, ELISA examination of patients' sera for anti-filaria IgG antibodies was performed. CONCLUSIONS: In the CR, five cases of human dirofilariasis caused by Dirofilaria repens were recorded during 2010-2014 (species determination for three of them was confirmed besides morphological also by DNA analysis). At least, three of the cases were of autochthonous origin (the patients are Czech citizens residing in South Moravian Region who have never travelled abroad). The findings confirm the natural setting of D. repens in South Moravian Region of the CR. Dirofilariasis should be therefore considered as endemic in this area where it may represent a significant risk factor for public health.
- MeSH
- Dirofilaria repens genetika izolace a purifikace metabolismus MeSH
- dirofilarióza diagnóza parazitologie patologie MeSH
- DNA helmintů genetika metabolismus MeSH
- dospělí MeSH
- ELISA MeSH
- imunoglobulin G krev MeSH
- kůže parazitologie patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- polymerázová řetězová reakce MeSH
- protilátky helmintové krev MeSH
- RNA ribozomální 5S genetika metabolismus MeSH
- subkutánní tkáň parazitologie patologie MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- dospělí MeSH
- Echinococcus multilocularis * MeSH
- echinokokóza plic epidemiologie farmakoterapie patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- zoonózy epidemiologie přenos MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- zvířata MeSH
The purpose of this work was to investigate the associations of serum cholesterol and apolipoproteins with measures of blood-brain barrier (BBB) permeability and CNS inflammation following the first clinical demyelinating event. This study included 154 patients [67% female; age, 29.5 ± 8.2 years (mean ± SD)] enrolled in a multi-center study of interferon β1-a treatment following the first demyelinating event. Blood and cerebrospinal fluid (CSF) were obtained at screening prior to treatment. A comprehensive serum lipid profile and multiple surrogate markers of BBB breakdown and CNS immune activity were obtained. Higher levels of serum HDL cholesterol (HDL-C) and ApoA-I were associated with lower CSF total protein level, CSF albumin level, albumin quotient, and CSF IgG level (all P ≤ 0.001 for HDL-C and all P < 0.01 for ApoA-I). HDL-C was also associated with CSF CD80+ (P < 0.001) and with CSF CD80+CD19+ (P = 0.007) cell frequencies. Higher serum HDL is associated with lower levels of BBB injury and decreased CD80+ and CD80+CD19+ cell extravasation into the CSF. HDL may potentially inhibit the initiation and/or maintenance of pathogenic BBB injury following the first demyelinating event.
- MeSH
- apolipoproteiny krev MeSH
- biologické markery krev MeSH
- demyelinizační nemoci MeSH
- dospělí MeSH
- HDL-cholesterol krev MeSH
- hematoencefalická bariéra účinky léků metabolismus patologie MeSH
- interferon beta terapeutické užití MeSH
- lidé MeSH
- longitudinální studie MeSH
- nemoci nervového systému krev MeSH
- roztroušená skleróza krev farmakoterapie patologie MeSH
- zánět krev MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- pozorovací studie MeSH
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
Parasitological investigations on red-breasted mergansers (Mergus serrator L.) in Iceland revealed digenean flukes of the family Schistosomatidae. Adult worms were detected in blood vessels of the large intestine and eggs were deposited in the mucosa and surrounded by granulomatous reactions. Traditional morphological methods showed that the flukes have very slender filiform bodies, males are equipped with a short gynaecophoric canal and both suckers and spatulate ends are present on each sex. Among characteristics of the flukes which render them morphologically distinct from other Trichobilharzia species are: i) males-well developed vesicula seminalis (v.s.) consisting of a short v.s. externa and a significantly longer (approx. 3 times) v.s. interna, unusually well developed genital papilla and localization of the first testis a relatively long distance posterior to the gynaecophoric canal; ii) eggs-small and elongated with slightly rounded poles and a short terminal spine. DNA taxonomic techniques confirmed that a new species had been identified, Trichobilharzia mergi sp. n. The sequence data were deposited in GenBank under the accession numbers JX456151 to JX456172. Comparison of the results with our previously published data on characterization of DNA of cercariae isolated from freshwater lymnaeid snails showed that larval development of T. mergi is associated with Radix balthica L. (=Radix peregra Müller, 1774;=Radix ovata Draparnaud, 1805).
- MeSH
- Anseriformes parazitologie MeSH
- fylogeneze MeSH
- infekce červy třídy Trematoda parazitologie veterinární MeSH
- molekulární sekvence - údaje MeSH
- nemoci ptáků parazitologie MeSH
- ribozomální DNA chemie genetika MeSH
- Schistosomatidae klasifikace genetika izolace a purifikace MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- střeva krevní zásobení parazitologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Island MeSH
Cercarial dermatitis (swimmer's itch) is a common non-communicable water-borne disease. It is caused by penetration of the skin by larvae (cercariae) of schistosomatid flukes and develops as a maculopapular skin eruption after repeated contacts with the parasites. The number of outbreaks of the disease is increasing, and cercarial dermatitis can therefore be considered as an emerging problem. Swimmer's itch is mostly associated with larvae of the bird schistosomes of Trichobilharzia spp. Recent results have shown that mammalian infections (including man) manifest themselves as an allergic reaction which is able to trap and eliminate parasites in the skin. Studies on mammals experimentally infected by bird schistosome cercariae revealed, however, that during primary infection, parasites are able to escape from the skin to the lungs or central nervous system. This review covers basic information on detection of the infectious agents in the field and the clinical course of the disease, including other pathologies which may develop after infection by cercariae, and diagnosis of the disease.
- MeSH
- centrální nervový systém mikrobiologie MeSH
- cerkárie imunologie MeSH
- dermatitida diagnóza imunologie parazitologie MeSH
- kůže mikrobiologie patologie MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- plavání MeSH
- plíce mikrobiologie MeSH
- Schistosoma MeSH
- schistosomóza komplikace diagnóza imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
