Tkáňové expandéry jsou od osmdesátých let 20. století akceptovanou chirurgickou technikou v řešení traumatických, pooperačních a jiných defektů nebo nedostatku tkání. Ve vlasaté části hlavy se zavádí buď podkožně, nebo subgaleárně nad periost, a umožňují tak získat kožní lalok včetně vlasových folikulů. Jednou z největších komplikací je špatný výběr místa pro tkáňový expandér. Na kazuistice ukazujeme, že k plánování jeho vhodného umístění může pomoci 3D rekonstrukce z CT a následně 3D tisk.
Tissue expanders have been an accepted surgical technique in the treatment of traumatic, post-operative and other defects and loss of tissue since the 1980s. The expander is inserted in the hairy part of the scalp either subcutaneously or subgaleally above the periosteum, thus enabling the skin lobe, including hair follicles, to develop normally. One of the major complications is the poor choice of location for the tissue expander. In the case report, we will present that 3D modeling from CT and subsequent 3D printing can help to plan its most suitable location.
- MeSH
- Printing, Three-Dimensional MeSH
- Carcinoma, Basal Cell surgery MeSH
- Middle Aged MeSH
- Humans MeSH
- Scalp surgery MeSH
- Tissue Expansion Devices * MeSH
- Imaging, Three-Dimensional MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
3D printing seems to be the technology of the future for the preparation of metallic implants. For such applications, corrosion behaviour is pivotal. However, little is published on this topic and with inconsistent results. Therefore, we carried out a complex study in which we compared two techniques of the 3D printing technology - selective laser melting and electron beam melting. The corrosion behaviour was studied in physiological solution by standard electrochemical techniques and susceptibility to localised corrosion was estimated too. All samples showed typical passive behaviour. Localised corrosion was shown to be possible on the original as-printed surfaces. Corrosion experiments were repeated tree times. To reveal possible negative effects of 3D printing on cytocompatibility, direct in vitro tests were performed with U-2 OS cells. The cells showed good viability and proliferation, but their growth was impeded by surface unevenness. Our results suggest that both techniques are suitable for implants production. Statistical evaluation was performed by ANOVA followed by Tukey's test.
The authors report on three cases in which a custom-made 3D printed titanium acetabular component of total hip arthroplasty was used to manage an advanced acetabular bone defect with pelvic discontinuity. The implant surface structure impeded long-term bone integration. Nonetheless, the stable bridging of the acetabular defect resulted in full integration of impacted bone allografts at the base of the implant. The pelvic continuity was restored within 12 months after surgery, and thus the acetabulum was prepared for potential further implantation of a standard revision acetabular component. Only one of the three female patients underwent a revision surgery at 18 months after surgery, the other two female patients were satisfied to such a degree with the clinical outcome at 6 years and 5 years, respectively, after surgery that they refused to undertake the revision surgery, despite X-ray images showing signs of loosening of the custom-made titanium acetabular component. The authors concluded that the implantation technique of three-point fixed custom-made 3D printed acetabular component made of titanium combined with impaction grafting of the acetabular base is a good alternative in managing the advanced bone defects of acetabulum with pelvic discontinuity after the failure of total hip arthroplasty. Even though inadequate surface porosity of the thus produced component did not allow its permanent osteointegration, the assembly was stable enough to allow the bone allografts to rebuild and restore continuity of the pelvis and facilitated future implantation of the standard revision acetabular component. Key words: 3D printing, individual acetabular component, titanium, total hip prosthesis, revision hip arthroplasty, acetabular reconstruction, custom-made implants.
- MeSH
- Printing, Three-Dimensional MeSH
- Acetabulum diagnostic imaging surgery MeSH
- Hip Prosthesis * MeSH
- Humans MeSH
- Arthroplasty, Replacement, Hip * MeSH
- Follow-Up Studies MeSH
- Prosthesis Design MeSH
- Reoperation MeSH
- Prosthesis Failure MeSH
- Titanium MeSH
- Treatment Outcome MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
Three-dimensional (3D) printing technology offers attractive possibilities for many fields. In electrochemistry, 3D printing technology has been used to fabricate customized 3D-printed electrodes as a platform to develop bio/sensing, energy generation and storage devices. Here, we use a 3D-printed graphene/polylactic (PLA) electrode made by additive manufacturing technology and immobilize horseradish peroxidase (HRP) to create a direct electron transfer enzyme-based biosensors for hydrogen peroxide detection. Gold nanoparticles are included in the system to confirm and facilitate heterogeneous electron transfer. This work opens a new direction for the fabrication of third-generation electrochemical biosensors using 3D printing technology, with implications for applications in the environmental and biomedical fields.
Článok predstavuje a hodnotí prelomové technologické postupy v ortodoncii: skutočný a pseudo 3D záznam, ich spracovanie a vizualizácie. Popisuje postup od celohlavového záznamu CBCT iCAT cez export do stereolitografického formátu (STL) až po jeho 3D tlač. Článok hodnotí možnosti manipulácie s týmto 3D záznamom a tiež sa detailnejšie sa venuje téme „rapid prototypingu" a perspektívam klinického využitia rôznych variant 3D tlače vrátane tlače biokompatibilnými materiálmi, napríklad titánom (AM/ EMB).
The article introduces and reviews revolutionary technologies and procedures in orthodontics: true ar pseudo 3D record, its processing and visualization. In particular, it describes procedure from CBCT iCATTM scanning through its export to stereolithographic (STL) format and final 3D print. In addition, the article reviews possibilities of 3D CBCT volume and brings the current view on rapid prototyping and perspectives of various 3D print techniques in clinical orthodontics including printing by biocompatible materials like titanium (AM/EBM).
- MeSH
- Printing, Three-Dimensional instrumentation MeSH
- Technology, Dental MeSH
- Child MeSH
- Adult MeSH
- Humans MeSH
- Malocclusion diagnostic imaging MeSH
- Dental Impression Materials MeSH
- Adolescent MeSH
- Imaging, Three-Dimensional MeSH
- Models, Dental * classification MeSH
- Dental Impression Technique classification instrumentation MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Humans MeSH
- Adolescent MeSH
- Publication type
- Evaluation Study MeSH
Repairing and regenerating damaged tissues or organs, and restoring their functioning has been the ultimate aim of medical innovations. 'Reviving healthcare' blends tissue engineering with alternative techniques such as hydrogels, which have emerged as vital tools in modern medicine. Additive manufacturing (AM) is a practical manufacturing revolution that uses building strategies like molding as a viable solution for precise hydrogel manufacturing. Recent advances in this technology have led to the successful manufacturing of hydrogels with enhanced reproducibility, accuracy, precision, and ease of fabrication. Hydrogels continue to metamorphose as the vital compatible bio-ink matrix for AM. AM hydrogels have paved the way for complex 3D/4D hydrogels that can be loaded with drugs or cells. Bio-mimicking 3D cell cultures designed via hydrogel-based AM is a groundbreaking in-vivo assessment tool in biomedical trials. This brief review focuses on preparations and applications of additively manufactured hydrogels in the biomedical spectrum, such as targeted drug delivery, 3D-cell culture, numerous regenerative strategies, biosensing, bioprinting, and cancer therapies. Prevalent AM techniques like extrusion, inkjet, digital light processing, and stereo-lithography have been explored with their setup and methodology to yield functional hydrogels. The perspectives, limitations, and the possible prospects of AM hydrogels have been critically examined in this study.
- MeSH
- Printing, Three-Dimensional MeSH
- Bioprinting methods MeSH
- Cell Culture Techniques MeSH
- Hydrogels * chemistry MeSH
- Drug Delivery Systems MeSH
- Humans MeSH
- Cell Culture Techniques, Three Dimensional methods MeSH
- Tissue Engineering * methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
Due to a broad spectrum of endodontic rotary instruments on the market and no standardised protocol for comparing their mechanical properties, it can be challenging for clinician to choose proper instruments. In vitro studies using resin blocks with artificial canals can offer many valuable information because of their uniformity compared to studies performed on extracted teeth. To improve precision and reproducibility of artificial canals, 3D printing was used in this study to manufacture endodontic test block samples. 20 commercially available endodontic blocks Endo-Training-Bloc-J by Dentsply Sirona were tested. The mean values of the measured parameters were used for a 3D CAD model of their replicas. 20 copies of the endodontic training blocks were printed from acrylic resin (VeroClear-RGD810, Stratasys, Eden Prairie, USA) using the 3D printer Objet30 Pro (Stratasys, Eden Prairie, USA). The key dimensions of the commercial blocks and the 3D printed blocks were measured under and compared using t - test and Levene's test for equality of variances. The profiles of the 3D printed artificial canals showed significantly lower dimensional variability when compared with the commercial blocks. 3D polyjet printing proved to be a precise and reproducible method for production of blocks for testing endodontic rotary instruments.
- MeSH
- Printing, Three-Dimensional MeSH
- Endometriosis * MeSH
- Tooth Extraction MeSH
- Humans MeSH
- Reproducibility of Results MeSH
- Research Design * MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
Simple analytical devices suitable for the analysis of various biochemical and immunechemical markers are highly desirable and can provide laboratory diagnoses outside standard hospitals. This study focuses on constructing an easily reproducible do-it-yourself ELISA plate reader biosensor device, assembled from generally available and inexpensive parts. The colorimetric biosensor was based on standard 96-well microplates, 3D-printed parts, and a smartphone camera as a detector was utilized here as a tool to replace the ELISA method, and its function was illustrated in the assay of TNFα as a model immunochemical marker. The assay provided a limit of detection of 19 pg/mL when the B channel of the RGB color model was used for calibration. The assay was well correlated with the ELISA method, and no significant matrix effect was observed for standard biological samples or interference of proteins expected in a sample. The results of this study will inform the development of simple analytical devices easily reproducible by 3D printing and found on generally available electronics.
Úvod a cíle práce: Sádrové studijní modely jsou standardem uchovávání dat pacienta ve 3D podobě. S rozvojem digitálních technologií se vyvinuly také možnosti, jak z digitálních dat vytvořit fyzický objekt – 3D tiskem. Cílem této práce bylo: (1) srovnat přesnost lineárních měření na sádrových studijních modelech a jejich 3D rekonstruovaných kopiích, (2) zhodnotit nové možnosti, výhody a nevýhody použití 3D tisku při použití ve stomatologii a (3) zjistit, do jaké míry mohou 3D tištěné modely nahradit tradiční modely. Materiál a metody: Deset sádrových studijních modelů ortodontického oddělení Stomatologické kliniky dětí a dospělých 2. LF UK a FN Motol bylo naskenováno a digitální data se použila pro open source 3D tiskárnu RepRap. Na modelech z tiskárny bylo provedeno měření stejných vzdáleností v rovinách x, y a z, jako na tradičních sádrových modelech. Získaná data se statisticky vyhodnotila. Výsledky: 3D tisk je vhodnou metodou k převodu digitálních dat do fyzické podoby. 3D repliky vykazují výhodnější vlastnosti než tradiční sádrové modely zejména z hlediska mechanické odolnosti a možnosti získání fyzického modelu jen v případě potřeby, především pro dlouhodobou kontrolu terapie u pacienta či pro forenzní řízení. Studie prokázala, že 3D tisky mohou být alternativou k tradičním sádrovým modelům a je možné na nich provést přesnou analýzu rozměrů zubních oblouků.
Introduction: Plaster casts present golden standard in keeping patients data in 3D. The aim of this work was (1) to compare linear measurements made on traditional plaster casts and 3D printed copies of dental plaster casts, (2) to find new possibilities of reconstruction of digital data, its reliability based on various criteria and (3) to determine whether 3D printed copies obtained using open source system RepRap can replace traditional plaster casts in dental practice. Material and methods: 10 dental plaster casts from the Orthodontic department, Department of stomatology for children and adults, 2nd Faculty of medicine, Charles University Prague, Czech Republic were scanned by inEos Blue scanner and printed on 3D printer RepRap. Linear measurements between selected points on the dental arches of upper and lower jaws on plaster casts and their 3D copies were recorded and statistically analyzed. Results: Use of 3D printed copies has many advantages over use of traditional plaster casts, mainly mechanical durability and keeping the data in digital form unless needed for example for forensic reasons. The precision and accuracy of the RepRap 3D printed copies of plaster casts were confirmed based on the statistical analysis. Our study proved that 3D printed copies can replace traditional plaster casts and it is possible to use them for detailed analysis.
- MeSH
- Humans MeSH
- Orthodontics MeSH
- Calcium Sulfate MeSH
- Statistics as Topic MeSH
- Printing * methods MeSH
- Imaging, Three-Dimensional * MeSH
- Models, Dental * classification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
