DHA. Dotaz Zobrazit nápovědu
Úvod: Vliv eikospentaneové kyseliny a dokosahexaneové kyseliny na imunitní reakce je studován velmi intenzivně. Cílem studie bylo zjistit, zda-li ovlivňuje DHA a EPA hladiny vybraných cytokinů Th1 odpovědi (IL-2, IL12-p70 a INF-g), a Th2 odpovědi (IL-4 a IL-6) a regulační cytokiny (IL- 10 a TGF-β1) a srovnání těchto změn u nemocných po odstranění tonzil pro chronický zánět (včetně infekce H. pylori). Materiál a metodika: Pacienti byli rozděleni do tří hlavních skupin a jedné kontrolní skupiny. Odběry byly provedeny před zahájením operace nebo před začátkem podávání EPA a DHA. Po 15 týdnech byl prováděn druhý odběr. Dávkování 540 mg EPA a 360 mg DHA bylo rozděleno do tří dávek denně. Ze získaných vzorků krvi byla odebrána plazma ke stanovení cytokinů. Jejich koncentrace byla následně změřena metodou protilátkových čipů Quantibody Custom Arrays. Statistické zpracování proběhlo za pomoci programu StatGraphics multifaktoriální analýzou ANOVA. Výsledky: Statisticky významná změna hladiny byla prokázána u cytokinu IL12-p70, kde se významnost potvrdila souběžně v obou částech měření. V první části byla zaznamenána signifikance p=0,04, a to mezi jednotlivými skupinami. V druhé části se objevil signifikantní výsledek (p=0,01), a to mezi stavem před a po podání EPA a DHA. Změna hladiny byla zjištěna i u odpovědi TH2, a to IL-6 po podávání EPA a DHA (korelační koeficient 0,48) a IL-4, kde jsou změny výraznější (korelační koeficient 0,77). Závěr: Cytokin IL12-p70 lze považovat za nejvíce ovlivněný podáním EPA a DHA a může mít vliv na odpověď Th1 a stimulaci Nk buněk.
Introduction: The effect of eikospentan acid (EPA) and dokosahexan acid (DHA) on immune reactions was very intensely studied. The aim of this study was to clarify the impact of chronic Helicobacter pylori infection and EPA and DHA levels on Th1 cytokines (IL-2, IL12-p70, INF-g), Th2 cytokines (IL-4, IL-6) and T reg cytokines (IL-10, TGF-b1) in patient after tonsillectomy. Materials and methods: Patients were sorted into three main groups and one control group. Sampling was performed before the start of surgery or before the start of EPA and DHA administration. The second sampling was performed after 15 weeks in all main groups. The daily dosage of 540 mg of EPA and 360 mg of DHA was divides into three doses . The cytokines were analysed from the blood plasma. The concentation of the cytokines was quantified by Quantibody Custom Array. Statistical analysis was performed by using StatGraphics programme – multifactorial analysis ANOVA. Results: Statistically significant result was obtained in cytokine IL12-p70 in both measurements. In first sampling (2012) was the significance p=0.04 among the individual groups. In the second sampling the significance was p=0.01 in the phase before and after preparate administration. Dependence on IL-6 was prooved after the perparate admonistration (correlative coeficient 0.48), on IL-4 was dependence little bit higher (correlative coeficient 0.77). Conclusion: Cytokine IL12-p70 seems to be the main regulatory cytokine, thus it is possible to think about Th1 and NK cells stimulation.
- MeSH
- chronická nemoc MeSH
- infekce vyvolané Helicobacter pylori MeSH
- interferon gama imunologie MeSH
- interleukin-12 imunologie MeSH
- interleukin-4 imunologie MeSH
- interleukin-6 imunologie MeSH
- kyselina eikosapentaenová * aplikace a dávkování MeSH
- kyseliny dokosahexaenové * aplikace a dávkování MeSH
- lidé MeSH
- pilotní projekty MeSH
- regresní analýza MeSH
- Th1 buňky imunologie MeSH
- Th2 buňky imunologie MeSH
- tonzilektomie MeSH
- tonzilitida * chirurgie imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- fyziologie výživy kojenců fyziologie účinky záření MeSH
- fyziologie výživy v těhotenství fyziologie účinky léků MeSH
- kyseliny dokosahexaenové aplikace a dávkování terapeutické užití MeSH
- lidé MeSH
- potravní doplňky klasifikace využití MeSH
- těhotenství MeSH
- Check Tag
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
The fads2 gene encoding delta6-desaturase, the rate-limiting enzyme of the LCPUFA biosynthesis is expressed in astrocytes. Dietary fatty acids, which cross the blood-brain barrier, may regulate the transcription of lipogenic enzymes through activation of transcription factors such as peroxisome proliferator-activated receptors (PPARs). The PPARs form the transcription complex with retinoid X receptors (RXRs) that are activated by 9-cis retinoic acid, a metabolite of vitamin A (VA). The study examines whether challenge of astrocytes with VA, prior 24-h treatment with palmitic acid (PA), alpha-linolenic acid (ALA) or docosahexaenoic acid (DHA) has the effect on the FADS2 expression. RT-qPCR showed that in astrocytes not challenged with VA, PA increased fads2 gene expression and DHA decreased it. However, in VA-primed astrocytes, PA doubled the FADS2 mRNA levels, while DHA increased fads2 gene expression, oppositely to non-primed cells. Furthermore, similar changes were seen in VA-primed astrocytes with regard to delta6-desaturase protein levels following PA and DHA treatment. ALA did not have any effect on the FADS2 mRNA and protein levels in either VA-primed or non-primed astrocytes. These findings indicate that in the presence of vitamin A, DHA upregulates fads2 gene expression in astrocytes.
- MeSH
- astrocyty účinky léků metabolismus MeSH
- exprese genu MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- kyseliny dokosahexaenové farmakologie MeSH
- mozková kůra účinky léků metabolismus MeSH
- novorozená zvířata MeSH
- potkani Wistar MeSH
- stearyl-CoA-desaturasa biosyntéza genetika MeSH
- upregulace účinky léků fyziologie MeSH
- vitamin A farmakologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The n-3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), exert hypolipidemic effects and prevent development of obesity and insulin resistance in animals fed high-fat diets. We sought to determine the efficacy of alpha-substituted DHA derivatives as lipid-lowering, antiobesity, and antidiabetic agents. C57BL/6 mice were given a corn oil-based high-fat (35% weight/weight) diet (cHF), or cHF with 1.5% of lipids replaced with alpha-methyl DHA ethyl ester (Substance 1), alpha-ethyl DHA ethyl ester (Substance 2), alpha,alpha-di-methyl DHA ethyl ester (Substance 3), or alpha-thioethyl DHA ethyl ester (Substance 4) for 4 months. Plasma markers of glucose and lipid metabolism, glucose tolerance, morphology, tissue lipid content, and gene regulation were characterized. The cHF induced obesity, hyperlipidemia, impairment of glucose homeostasis, and adipose tissue inflammation. Except for Substance 3, all other substances prevented weight gain and Substance 2 exerted the strongest effect (63% of cHF-controls). Glucose intolerance was significantly prevented (~67% of cHF) by both Substance 1 and Substance 2. Moreover, Substance 2 lowered fasting glycemia, plasma insulin, triacylglycerols, and nonesterified fatty acids (73, 9, 47, and 81% of cHF-controls, respectively). Substance 2 reduced accumulation of lipids in liver and skeletal muscle, as well as adipose tissue inflammation associated with obesity. Substance 2 also induced weight loss in dietary obese mice. In contrast to DHA administered either alone or as a component of the EPA/DHA concentrate (replacing 15% of dietary lipids), Substance 2 also reversed established glucose intolerance in obese mice. Thus, Substance 2 represents a novel compound with a promising potential in the treatment of obesity and associated metabolic disturbances.
- MeSH
- dietní tuky MeSH
- energetický příjem MeSH
- glukosa metabolismus MeSH
- glukózový toleranční test MeSH
- hmotnostní přírůstek MeSH
- hypolipidemika terapeutické užití MeSH
- kyseliny dokosahexaenové terapeutické užití MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- obezita farmakoterapie prevence a kontrola MeSH
- polymerázová řetězová reakce MeSH
- porucha glukózové tolerance farmakoterapie prevence a kontrola MeSH
- RNA genetika izolace a purifikace MeSH
- triglyceridy metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
OBJECTIVES: The aim of the study was to determine whether increased intake of DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid) would affect the weight loss or the various biochemical parameters in the blood of obese children following dietary/physical intervention. There were 120 obese (BMIs≥30 kg/m(2); mean 33.5 ± 3.9) children included in this randomized crossover study; aged 8-12 years (10.0 ± 1.9). METHODS: The children consumed an extra 300 mg DHA and 42 mg EPA (Haliborange ®) daily for a period of 3 weeks. Anthropometric and biochemical parameters were measured and documented for each of the subjects at the beginning of the study, after three weeks of treatment and at the end of the study. RESULTS: The daily consumption of 300 mg DHA and 42 mg of EPA was associated with decreased body weight (with DHA: 86.4 ± 19.6 to 80.8 ± 20.4 kg vs. without DHA: 85.6 ± 20.8 to 80.9 ± 19.9 kg; p<0.005) and total cholesterol concentration (with DHA: 3.72 ± 0.78 to 3.32 ± 0.53 mmol/l vs. without DHA: 3.74 ± 0.78 to 3.56 ± 0.56 mmol/l; p<0.05 and respectively with DHA). CONCLUSION: Daily consumption of 300 mg DHA and 42 mg EPA (Haliborange®) for 3 weeks leads to an improvement of the anthropometric and lipid parameters in obese children following dietary physical intervention.
- MeSH
- antropometrie MeSH
- C-reaktivní protein analýza MeSH
- dítě MeSH
- hmotnostní úbytek účinky léků MeSH
- klinické křížové studie MeSH
- kyseliny dokosahexaenové farmakologie terapeutické užití MeSH
- kyseliny mastné omega-3 terapeutické užití MeSH
- lidé MeSH
- lipidy krev MeSH
- metabolismus lipidů účinky léků MeSH
- obezita dietoterapie farmakoterapie metabolismus MeSH
- tělesná hmotnost účinky léků fyziologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- randomizované kontrolované studie MeSH
BACKGROUND: Experimental, epidemiological and clinical data substantiate the beneficial role of n-3 polyunsaturated fatty acids (PUFAs) in preventing inflammation and cancer of the colon. This review covers the unsaturated docosahexaenoic fatty acid (DHA), describes some of its important cellular and molecular mechanisms, its interaction with another dietary lipid, butyrate and with endogenous apoptotic regulators of the tumour necrosis factor (TNF) family. We also discuss the clinical impact of this knowledge and the use of these lipids in colon cancer prevention and treatment. RESULTS: From the literature, DHA has been shown to suppress the growth, induce apoptosis in colon cancer cells in vitro and decrease the incidence and growth of experimental tumours in vivo. Based on these data and our own experimental results, we describe and discuss the possible mechanisms of DHA anticancer effects at various levels of cell organization. We show that DHA can sensitize colon cancer cells to other chemotherapeutic/chemopreventive agents and affect the action of physiological apoptotic regulators of the TNF family. CONCLUSION: Use of n-3 PUFAs could be a relatively non-toxic form of supportive therapy for improving colon cancer treatment and slowing down or preventing its recurrence. However, it is necessary to use them with caution, based on solid scientific evidence of their mechanisms of action from the molecular to the cellular and organism levels.
- MeSH
- antitumorózní látky farmakologie MeSH
- apoptóza účinky léků MeSH
- kolon cytologie účinky léků MeSH
- kyseliny dokosahexaenové farmakologie fyziologie MeSH
- lidé MeSH
- nádory tračníku patologie MeSH
- proliferace buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Publikační typ
- novinové články MeSH
- MeSH
- Alzheimerova nemoc dietoterapie MeSH
- klinické zkoušky jako téma MeSH
- kognice MeSH
- kyseliny mastné omega-3 aplikace a dávkování terapeutické užití MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
AIMS: Male infertility has been associated with imbalance in the polyunsaturated fatty acids arachidonic acid/docosahexaenoic acid (AA/DHA) ratio. This studyAIMed to assess whether use of the potent antioxidant, lycopene, would affect this imbalance in seminal plasma and to test whether modulation of the AA/DHA ratio correlates with male fertility. METHODS: The study included 44 males from infertile couples who were treated with lycopene for three months before scheduled IVF treatment. The control group was 13 fertile males who were also treated with lycopene for three months. AA and DHA levels were measured in seminal plasma before and after treatment. The outcome of IVF treatment after lycopene use in of males from infertile couples was recorded. RESULTS: The subjects were subdivided into 21 normozoospermic males from couples with idiopathic infertility and 23 males with semen abnormalities. Prior to the treatment with lycopene, the AA/DHA ratios in both subgroups of patients were significantly higher than in fertile controls and improved following treatment with lycopene. Improvement was more marked in the normospermic males. Seven spontaneous pregnancies (16%) occurred before the scheduled IVF treatment and 15 couples (42%) achieved pregnancy after IVF. CONCLUSIONS: Three months of treatment with lycopene led to a significant improvement in the AA/DHA ratio in seminal plasma of males from infertile couples and facilitated the spontaneous as well as IVF conception.
- MeSH
- dospělí MeSH
- karotenoidy terapeutické užití MeSH
- kyselina arachidonová metabolismus MeSH
- kyseliny dokosahexaenové metabolismus MeSH
- lidé MeSH
- mužská infertilita farmakoterapie metabolismus MeSH
- oxidační stres účinky léků MeSH
- protozoální proteiny metabolismus MeSH
- sperma účinky léků metabolismus MeSH
- těhotenství MeSH
- transkripční faktory metabolismus MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
