Fibronectin
Dotaz
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- MeSH
- fibronektiny MeSH
- imunohistochemie MeSH
- lidé MeSH
- slezina cytologie imunologie patofyziologie MeSH
- Check Tag
- lidé MeSH
- MeSH
- bércové vředy terapie MeSH
- biomedicínský výzkum MeSH
- dospělí MeSH
- glykoproteiny terapeutické užití MeSH
- hojení ran MeSH
- lidé středního věku MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- Publikační typ
- kazuistiky MeSH
The authors examined by immunofluorescence the presence of collagen type IV and fibronectin in a group of nine patients with lichen ruber planus. Collagen iyipe IV was present in the form of granules in the dermis of six patients. Fibronectin was in the region of the dermoepidermal junction at the base of teeth, acanthotic epidermis and the adjacent papillary dermis. As control the authors used 10 specimens of healthy skin from the Clinic of Plastic Surgery in Košice. The authors assume that the mentioned findings of collagen IV and fibronectin reflect damage of the basal membrane zone in lichen ruber planus.
- MeSH
- bazální membrána MeSH
- fibronektiny analýza MeSH
- kolagen analýza MeSH
- lichen planus patologie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
Reviews of infectious diseases, ISSN 0162-0886 vol. 9, suppl. 4, July-August 1987
S317-S430 s. : il., tab., grafy ; 30 cm
- MeSH
- fibronektiny fyziologie MeSH
- infekce patofyziologie MeSH
- receptory fibronektinů MeSH
- Publikační typ
- souborné dílo MeSH
Autori zistovali imunohistochemicky výskyt fibronektínu a βl-integrínového retazca v skupme 11 pacientovi s erythema exsudativum multiforme v derme a epiderme. V derme zistili zvýšenú expresiu fibronektínu v jej papilárnej častí. Zvýšená expresia βl-integrínového retazca (CD29) hola zistená v oblasti hydropicky degenerovanej epidermy.
The authors assessed the immunohistochemical incidence of fibronectin and the βl-integrin chain in a group of 11 patients with erythema exsudativum multiforme in the dermis and epidermis. In the dermis they found an increased expression of fibronectin in the papillary part. An increased expression of the βl-integrin chain (CD29) was found in the area of the hydropically degenerated epidermis.
In this review protocols are described for studying protein tyrosine kinase signalling upon integrin-mediated cell adhesion. We have outlined detailed procedures for fibronectin-replating experiment, biochemical examination of the phosphotyrosine content of cellular proteins by immunoblotting using phosphorylation-specific antibodies or immunoprecipitation and analysis with general phosphotyrosine antibodies. Despite great advances that were made toward optimizing the described procedures, all these methods still remain in many respects an art, given the plentiful of variables and the extent to which the optimum conditions vary from one experimental condition to the other. Examples of performed experiments using the described procedures thus also include notes regarding variability of approaches based on experimental conditions.
Byl sledován výskyt fibronectinu v parafínových řezech vzorků popálené kůže ve srovnání s kůží intaktní a kůží vystavenou termickým změnám po smrti. K tomu byla užita imunohistochemická metoda neprime imunoperoxidasové reakce. U posmrině popálených vzorků byl patrný výpadek reakce fibronectinu v epidermo-dermálním spojení a ve stratum papillare. Ve vzorcích popálené kůže odebraných od jedinců zemřelých velmi krátce po vzniku popálenin nebyla prokázána aktivita fibronectinu. Také ve dvou pripadech, kdy pacienti zemřeli během několika hodin v důsledku těžkého popálení, nebyl zgištěn nápadný rozdfl v intenzitě aktivity fibronectinu, ve srovnám s intaktní kůží. Aktivita fibronectinu byla zvýšena u všech ostatních vzorků s dobou přežití od 30 minut do 5 týdnů a to v různé intenzitě a různé podobě distribuce. U popálenin I. a povrchových popálenin II. stupně byl fibronectin přítomný i v epidermis, ve stratum papillare se objevil spíš difusně nebo v podobě skvrn, kdežto ve stratum reticulare měl tendenci vytvářet síťové struktury mezi kolagenními vlákny. U hlubokých popálenin II. stupně a popálenin III. stupně se fibronectin ukládal predilekčně do okolí zachovaných cév a kožních adnex v j podobě jemných vláken. U 6 z 11 vzorků S dobou přežití od 7 do 21 dní byly fibroblasty uspořádané mezi jemnými kolagenními vlákny a některé z nich prezentovaly pozitivní aktivitu fibronectinu na povrchu. Hojné fibroblasty s jemně roztroušenými fibronectinovými skvrnami a obraz snížení aktivity fibronectinu ve zralejší granulační tkáni byly pozorovány u popálenin kůže 5 týdnů starých.
Occurence of fibronectin was detected in paraffin sections of bum skin samples, excised from surviving patients, as well as skin samples removed from the death persons, who succumbed bum wounds. The other groups of samples, used for comparison with the previous one, consisted of the intact skin excisions and skin samples post-mortem exposed to thermic changes. Indirect immunoperoxidase reaction was the immunohistochemical method used in all examinations. In post-mortem bum skin samples there was a loss of fibronectin activity in the epidermo-dermal junction area as well as in the papillary dermis. In bum skin samples, excised from patients who only survived for very short time their (serious skin) bums, no evidence of any fibronectin activity was seen. Also in two other cases, when patients - due to their severe bums - survived for several hours only, there were no conspicuous differences in the intensity of fibronectin activity seen in comparison with features fotmd in the intact skin samples. Fibronectin activity was, however, increased in all other burn skin samples, where the survival time of patients was from 30 minutes to 5 weeks and there were following differences in its intensity and also in its pattern of distribution. In the Ist-degree and in a superficial 2nd-degree skin bum wounds, fibronectin was also present in the epidermis. In the papillary dermis, fibronectin was distributed rather diffusely or in a spot-like pattern while in the reticular dermis, there was a tendency to form net-like structures among collagen fibers. In deeper 2nd-degree and in the 3rd-degree bum wounds, fibronectin was deposited in vicinity of blood vessels and skin appendages in a fibrillar pattern. In 6 out of 11 samples, where the survival time ranged from 7 to 21 days, fibroblasts were arranged among fine collagen fibers and some of these cells exhibited positive fibronectin activity on their surface. Numerous fibroblasts with finely scattered fibronectin spots and also a decreas of fibronectm activity were observed in more mature granidation tissue, present in bum skin samples where survival time of patients was five weeks.
Metabolism of fibronectin, the protein that plays a key role in the healing of wounds, is changed in the patients with diabetes mellitus. Fibronectin can interact with other proteins and proteoglycans and organise them to form the extracellular matrix, the basis of the granulation tissue in healing wounds. However, diabetic foot ulcers (DFUs) suffer from inadequate deposition of this protein. Degradation prevails over fibronectin synthesis in the proteolytic inflammatory environment in the ulcers. Because of the lack of fibronectin in the wound bed, the assembly of the extracellular matrix and the deposition of the granulation tissue cannot be started. A number of methods have been designed that prevents fibronectin degradation, replace lacking fibronectin or support its formation in non-healing wounds in animal models of diabetes. The aim of this article is to review the metabolism of fibronectin in DFUs and to emphasise that it would be useful to pay more attention to fibronectin matrix assembly in the ulcers when laboratory methods are translated to clinical practice.
- MeSH
- diabetes mellitus * metabolismus MeSH
- diabetická noha * terapie MeSH
- extracelulární matrix MeSH
- fibronektiny MeSH
- hojení ran MeSH
- hyperglykemie * metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
474 s. : obr., tab., přeruš.bibliogr.
