GM-CSF, granulocyte macrophage colony-stimulating factor Dotaz Zobrazit nápovědu

Přesná shoda Sémantické

Granulocyte-macrophage colony stimulating factor (GM-CSF) is considered to be the most effective immunostimulating factor for the construction of gene-engineered anti-cancer vaccines. In some tumour cells, this type of genetic modification has resulted in the loss of the oncogenic potential. This was not the case with bcr-abl-transformed mouse 12B1 cells. A cell line, designated 12B1/GM-CSF/cl-5 producing more than 100 ng/106 cells/24 h, displayed higher pathogenicity than the parental, non-transduced cells. Although the tumours induced by the parental 12B1 cells and 12B1/GM-CSF/cl-5 cells appeared nearly at the same time and then grew at an approximately equal rate, the latter mice were in a much poorer clinical condition. In these animals the growth of the tumours was associated with gradually increasing blood levels of GM-CSF. In both groups of animals splenomegaly was observed; it was much more pronounced in the case of 12B1/GM-CSF/cl-5-inoculated animals. While in the case of animals inoculated with the parental cells the splenomegaly was probably mainly due to infiltration with tumour cells, in the animals inoculated with the GM-CSF-secreting cells splenomegaly and derangement of parenchymal organs, such as lungs, liver and kidneys, were more complex, including congestion and infiltration with hemopoietic cells, predominantly immature cells of myeloid lineage. The most conspicuous of these changes was the hyperaemia of the lungs. No such alterations were seen in animals inoculated with the parental cells. On the other hand, the contents of T regulatory cells were comparable in both groups and they increased in parallel at the end of the observation period. When GM-CSF neutralizing antibody was administered to animals inoculated with the 12B1/GM-CSF/cl-5 cells, the pathological changes observed within the organs were suppressed, this proving that the overproduced GM-CSF and not any other substance, played the key role in their induction.

MeSH
bcr-abl fúzní proteiny genetika metabolismus MeSH
experimentální nádory patologie MeSH
faktor stimulující granulocyto-makrofágové kolonie krev imunologie sekrece MeSH
interleukin-2 sekrece MeSH
játra patologie MeSH
ledviny patologie MeSH
leukemie patologie MeSH
myši inbrední BALB C MeSH
myši MeSH
nádorové buněčné linie MeSH
neutralizující protilátky aplikace a dávkování MeSH
plíce patologie MeSH
regulační T-lymfocyty imunologie MeSH
slezina imunologie patologie MeSH
transplantace nádorů MeSH
tumor burden MeSH
zvířata MeSH
Check Tag
myši MeSH
ženské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH

Článek

Úspešná liečba agranulocytózy vyvolanej karbimazolom pomocou rekombinantného granulocyty-makrofágy kolónie stimulujúceho faktora
[Successful treatment of agranulocytosis induced by carbimazole by means of a recombinant factor stimulating granulocyte - macrophage colonies]

Vnitřní lékařství. 1995 ; Roč. 41 (č. 7) : s. 470-472.

ISSN 0042-773X
Medvik
Zdroj

MeSH
agranulocytóza MeSH
faktor stimulující granulocyto-makrofágové kolonie MeSH
hypertyreóza farmakoterapie komplikace MeSH
karbimazol aplikace a dávkování škodlivé účinky terapeutické užití MeSH
lidé středního věku MeSH
lidé MeSH
Check Tag
lidé středního věku MeSH
lidé MeSH
ženské pohlaví MeSH
Publikační typ
kazuistiky MeSH

Článek online

Granulocyte-macrophage colony-stimulating factor-producing tumour vaccines

Folia biologica (Praha). 1999 ; Roč. 45 (č. 5) : s. 173-177.

ISSN 0015-5500
Medvik
Zdroj

MeSH
experimentální nádory terapie MeSH
faktor stimulující granulocyto-makrofágové kolonie MeSH
genetická terapie metody MeSH
myši MeSH
protinádorové vakcíny MeSH
transfekce MeSH
transformované buněčné linie fyziologie transplantace MeSH
zvířata MeSH
Check Tag
myši MeSH
zvířata MeSH

Článek online

Activation of adenosine A3 receptors potentiates stimulatory effects of IL-3, SCF, and GM-CSF on mouse granulocyte-macrophage hematopoietic progenitor cells

... interleukin-3 (IL-3), stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF ...

Hofer, Michal
Autor Autorita
Pospíšil, Milan, 1929-
Autor Autorita
Holá, Jiřina
Autor Autorita
Štreitová, Denisa
Autor Autorita
Znojil, Vladimír, 1941-2008
Autor Autorita
Vacek, Antonín, 1929-2009
Autor Autorita

Physiological research. 2009 ; 58 (2) : 247-252.

ISSN 0862-8408
Medvik
Zdroj

Adenosine A(3) receptor agonist N(6)-(3-iodobenzyl)adenosine-5'-N-methyluronamide (IB-MECA) has been tested from the point of view of potentiating the effects of hematopoietic growth factors interleukin-3 (IL-3), stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF) on the growth of hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC) in suspension of normal mouse bone marrow cells in vitro. IB-MECA alone induced no GM-CFC growth. Significant elevation of numbers of GM-CFC evoked by the combinations of IB-MECA with IL-3, SCF, or GM-CSF as compared with these growth factors alone has been noted. Combination of IB-MECA with G-CSF did not induce significantly higher numbers of GM-CFC in comparison with G-CSF alone. Joint action of three drugs, namely of IB-MECA + IL-3 + GM-CSF, produced significantly higher numbers of GM-CFC in comparison with the combinations of IB-MECA + IL-3, IB-MECA + GM-CSF, or IL-3 + GM-CSF. These results give evidence of a significant role of selective activation of adenosine A(3) receptors in stimulation of the growth of granulocyte/ macrophage hematopoietic progenitor cells.

Článek online

Adenosine potentiates stimulatory effects on granulocyte-macrophage hematopoietic progenitor cells in vitro of IL-3 and SCF, but not those of G-CSF, GM-CSF and IL-11

... and cytokines, namely with granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating ...

Physiological research. 2006 ; 55 (5) : 591-596.

ISSN 0862-8408
Medvik
Zdroj

The aim of the studies was to ascertain if adenosine is able to co-operate with selected hematopoietic growth factors and cytokines, namely with granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF), interleukin-3 (IL-3), and interleukin-11 (IL-11), in inducing the growth of colonies from hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC) from normal bone marrow cells in vitro. Adenosine was found not to produce any colonies when present in the cultures as the only potential stimulator. All the tested cytokines and growth factors were observed to induce the growth of distinct numbers of GM-CFC colonies, with the exception of IL-11. When suboptimal concentrations of the evaluated cytokines and growth factors were tested in the cultures in which various concentrations of adenosine were concomitantly present, mutually potentiating effects were found in the case of IL-3 and SCF. These results confirm the role of adenosine in regulation of granulopoiesis and predict IL-3 and SCF as candidates for further in vivo studies of their combined administration with adenosine.