- MeSH
- biologická terapie * metody MeSH
- idiopatické střevní záněty * farmakoterapie MeSH
- lidé MeSH
- management farmakoterapie MeSH
- udržovací chemoterapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- směrnice pro lékařskou praxi MeSH
- MeSH
- biologická terapie metody škodlivé účinky MeSH
- idiopatické střevní záněty * farmakoterapie MeSH
- imunosupresiva škodlivé účinky terapeutické užití MeSH
- léková kontraindikace MeSH
- lidé MeSH
- reprodukční zdraví * MeSH
- těhotenství MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- směrnice pro lékařskou praxi MeSH
- MeSH
- biosimilární léčivé přípravky terapeutické užití MeSH
- dítě MeSH
- dospělí MeSH
- idiopatické střevní záněty * farmakoterapie MeSH
- inhibitory TNF terapeutické užití MeSH
- lidé MeSH
- těhotenství MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- směrnice pro lékařskou praxi MeSH
There is still a need for synthetic approaches that are much faster, easier to scale up, more robust and efficient for generating gold(I)-thiolates that can be easily converted into gold-thiolate nanoclusters. Mechanochemical methods can offer significantly reduced reaction times, increased yields and straightforward recovery of the product, compared to the solution-based reactions. For the first time, a new simple, rapid and efficient mechanochemical redox method in a ball-mill was developed to produce the highly luminescent, pH-responsive Au(I)-glutathionate, [Au(SG)]n. The efficient productivity of the mechanochemical redox reaction afforded orange luminescent [Au(SG)]n in isolable amounts (mg scale), usually not achieved by more conventional methods in solution. Then, ultrasmall oligomeric Au10-12(SG)10-12 nanoclusters were prepared by pH-triggered dissociation of [Au(SG)]n. The pH-stimulated dissociation of the Au(I)-glutathionate complex provides a time-efficient synthesis of oligomeric Au10-12(SG)10-12 nanoclusters, it avoids high-temperature heating or the addition of harmful reducing agent (e.g., carbon monoxide). Therefore, we present herein a new and eco-friendly methodology to access oligomeric glutathione-based gold nanoclusters, already finding applications in biomedical field as efficient radiosensitizers in cancer radiotherapy.
- Publikační typ
- časopisecké články MeSH
Diagnostika a liečba (eradikácia) Helicobactera pylori (Hp) je medicínskym aj ekonomickým problémom. Narastajúca rezistencia baktérie na makrolidy a metronidazol, novšie aj na chinolóny (levofloxacín) a nárast nákladov na diagnostiku či novozavádzané liečivá, nás nútia hľadať racionálne prístupy k liečbe Hp a dodržiavať európske aj národné odporúčania. Práca na novom odbornom usmernení z dielne MZ SR, hlavného odborníka pre gastroenterológiu a pracovnej skupiny pre Hp pri SGS zabrala viac ako rok. Tretie upravené a doplnené odporúčania pre diagnostiku a liečbu Hp potvrdilo nutnosť medziodborovej spolupráce.
Diagnostics and treatment of Helicobacter pylori (Hp) is a medical and economical problem as well. Rising resistance of bacteria towards macrolids and metronidazol, lately also to chinolons (Levofloxacin), together with a growth of costs needed for diagnostics or newly introduced medicaments, force us to look for the rational approach to Hp treatment that have to comply with European and national guidelines. Work on new technical directions from the workshop of Healthcare Ministry of the Slovak Republic, general expert for gastroenterology, and the working group for Hp at the SGS, has taken more than one year time. These revised and filled in guidelines for Hp diagnostics and treatment proved the necessity of cooperation among medical departments and branches.
Česká a slovenská gastroenterologie, ISSN 1210-7824 roč. 53, suppl., prosinec 1999
120 s. ; 32 cm
Allogeneic HSCT represents the only potentially curative treatment for very high risk (VHR) ALL. Two consecutive international prospective studies, ALL-SCT-(I)BFM 2003 and 2007 were conducted in 1150 pediatric patients. 569 presented with VHR disease leading to any kind of HSCT. All patients >2 year old were transplanted after TBI-based MAC. The median follow-up was 5 years. 463 patients were transplanted from matched donor (MD) and 106 from mismatched donor (MMD). 214 were in CR1. Stem cell source was unmanipulated BM for 330 patients, unmanipulated PBSC for 135, ex vivo T-cell depleted PBSC for 62 and cord-blood for 26. There were more advanced disease, more ex vivo T-cell depletion, and more chemotherapy based conditioning regimen for patients transplanted from MMD as compared to those transplanted from MSD or MD. Median follow up (reversed Kaplan Meier estimator) was 4.99 years, median follow up of survivals was 4.88, range (0.01-11.72) years. The 4-year CI of extensive cGvHD was 13 ± 2% and 17 ± 4% (p = NS) for the patients transplanted from MD and MMD, respectively. 4-year EFS was statistically better for patients transplanted from MD (60 ± 2% vs. 42 ± 5%, p < 0.001) for the whole cohort. This difference does not exist if considering separately patients treated in the most recent study. There was no difference in 4-year CI of relapse. The 4-year NRM was lower for patients transplanted from MD (9 ± 1% vs. 23 ± 4%, p < 0.001). In multivariate analysis, donor-type appears as a negative risk-factor for OS, EFS, and NRM. This paper demonstrates the impact of donor type on overall results of allogeneic stem cell transplantation for very-high risk pediatric acute lymphoblastic leukemia with worse results when using MMD stem cell source.
- MeSH
- akutní lymfatická leukemie * terapie MeSH
- dárci tkání MeSH
- dítě MeSH
- lidé MeSH
- nemoc štěpu proti hostiteli * MeSH
- předškolní dítě MeSH
- příprava pacienta k transplantaci MeSH
- prospektivní studie MeSH
- retrospektivní studie MeSH
- transplantace hematopoetických kmenových buněk * MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- předškolní dítě MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Stresová granula (Sgs) a procesní tělíska (PB) jsou mikroskopem viditelná ribonukleinová granula, která regulují translaci a rozklad RNA. Bylo identifikováno 101 lidských genů, nutných pro Sg a 39 pro PB a 31 pro jejich koordinaci. Některé složky hexosamin-biosyntetické cesty reverzibilně modifikují proteiny s O-N-acetylglukosaminem (O-GlcNAc) v odpovědi na stres. Ukazuje se, že právě modifikace O GlcNAc v translačním mechanismu je nutná pro agregaci nepřeložených mesenžerových ribonukleinových proteinů do Sgs. Nedostatek enzymů hexosaminové biosyntetické cesty v bujících kvasinkách může přispívat k rozdílům mezi savčími Sgs a příbuznými kvasinkovými tělísky EGP.
Česká a slovenská gastroenterologie a hepatologie, ISSN 1213-323X roč. 59, suppl. 2, listopad 2005
118 s. ; 28 cm
- MeSH
- gastroenterologie metody trendy MeSH
- gastrointestinální nemoci MeSH
- nemoci jater MeSH
- Publikační typ
- abstrakt z konference MeSH
- sborníky MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- gastroenterologie
- hepatologie
Stress granules (SGs), hallmarks of the cellular adaptation to stress, promote survival, conserve cellular energy, and are fully dissolved upon the cessation of stress treatment. Different stresses can initiate the assembly of SGs, but arsenite and heat are the best studied of these stresses. The composition of SGs and posttranslational modifications of SG proteins differ depending on the type and severity of the stress insult, methodology used, cell line, and presence of overexpressed and tagged proteins. A group of 18 proteins showing differential localization to SGs in heat- and arsenite-stressed mammalian cell lines is described. Upon severe and prolonged stress, physiological SGs transform into more solid protein aggregates that are no longer reversible and do not contain mRNA. Similar pathological inclusions are hallmarks of neurodegenerative diseases. SGs induced by heat stress are less dynamic than SGs induced by arsenite and contain a set of unique proteins and linkage-specific polyubiquitinated proteins. The same types of ubiquitin linkages have been found to contribute to the development of neurodegenerative disorders such as Parkinson disease, Alzheimer disease, and amyotrophic lateral sclerosis (ALS). We propose heat stress-induced SGs as a possible model of an intermediate stage along the transition from dynamic, fully reversible arsenite stress-induced SGs toward aberrant SGs, the hallmark of neurodegenerative diseases. Stress- and methodology-specific differences in the compositions of SGs and the transition of SGs to aberrant protein aggregates are discussed. This article is categorized under: RNA in Disease and Development > RNA in Disease RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes RNA Export and Localization > RNA Localization.
