enveloped virus
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BACKGROUND: Healthcare-acquired infections by pathogenic microorganisms including viruses represent significant health concern worldwide. Next to direct transmission from person-to-person also indirect transmission from contaminated surfaces is well documented and important route of infections. Here, we tested antiviral properties of hybrid coating containing silver, copper and zinc cations that was previously shown to be effective against pathogenic bacteria including methicillin-resistant Staphylococcus aureus. Hybrid coatings containing silver, copper and zinc cations were prepared through radical polymerization via sol-gel method and applied on glass slides or into the wells of polymethylmethacrylate plates. A 10 μl droplet of several viruses such as human immunodeficiency virus type 1 (HIV-1), influenza, dengue virus, herpes simplex virus, and coxsackievirus was added to coated and uncoated slides or plates, incubated usually from 5 to 240 min and followed by titer determination of recovered virus. RESULTS: Scanning electron microscopy analysis showed better adhesion of coatings on glass surfaces, which resulted in 99.5-100 % HIV-1 titer reduction (3.1 ± 0.8 log10TCID50, n = 3) already after 20 min of exposure to coatings, than on coated polymethylmethacrylate plates with 75-100 % (1.7 ± 1.1 log10TCID50, n = 3) and 98-100 % (2.3 ± 0.5 log10TCID50, n = 3) HIV-1 titer reduction after 20 and 120 min of exposure, respectively. Slower virucidal kinetics was observed with other enveloped viruses, where 240 min exposure to coated slides lead to 97 % (dengue), 100 % (herpes simplex) and 77 % (influenza) reduction in virus titers. Interestingly, only marginal reduction in viral titer after 240 min of exposure was noticed for non-enveloped coxsackie B3 virus. CONCLUSIONS: Our hybrid coatings showed virucidal activity against HIV and other enveloped viruses thus providing further findings towards development of broad-spectrum antimicrobial coating suitable for surfaces in healthcare settings.
- MeSH
- antivirové látky chemie farmakologie MeSH
- HIV infekce MeSH
- HIV-1 účinky léků MeSH
- infekce spojené se zdravotní péčí prevence a kontrola virologie MeSH
- kationty chemie farmakologie MeSH
- kontaminace zdravotnického vybavení prevence a kontrola MeSH
- lidé MeSH
- měď chemie farmakologie MeSH
- stříbro chemie farmakologie MeSH
- viry účinky léků MeSH
- vybavení a zásoby nemocnice virologie MeSH
- zinek chemie farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Influenza spreads around the world in seasonal epidemics and it is caused by a variety of species and strains of viruses, in any given year some strains can die out while others create epidemics, while yet another strain can cause a pandemic. The peptides may present the new generation of antiviral drugs with the broad spectrum of activity. The antiviral effects depend on their structure as well as the target part of the virus. We showed the manner of action of peptides on the influenza virus. The entry blocker peptides interact with hemagglutinin and inhibit the viral fusion. Furthermore the peptides are capable to disrupt viral envelope or block the viral replication. The perceptivity of therapeutic peptides is supported by wild abilities of their synthesis, possibility of modifications, synthesis for specific action, minimizing the emergence of resistance. Clearly, all these studies are promising, and need to be expanded.
- MeSH
- antivirové látky farmakologie chemická syntéza MeSH
- chřipka lidská farmakoterapie MeSH
- hemaglutininové glykoproteiny viru chřipky účinky léků MeSH
- lidé MeSH
- peptidy farmakologie chemie terapeutické užití MeSH
- proteiny virového obalu MeSH
- replikace viru účinky léků MeSH
- RNA virová účinky léků MeSH
- virus chřipky A účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
This work is dedicated to the study of the variability of the main antigenic envelope protein E among different strains of tick-borne encephalitis virus at the level of physical and chemical properties of the amino acid residues. E protein variants were extracted from then NCBI database. Four amino acid residues properties in the polypeptide sequences were investigated: the average volume of the amino acid residue in the protein tertiary structure, the number of amino acid residue hydrogen bond donors, the charge of amino acid residue lateral radical and the dipole moment of the amino acid residue. These physico-chemical properties are involved in antigen-antibody interactions. As a result, 103 different variants of the antigenic determinants of the tick-borne encephalitis virus E protein were found, significantly different by physical and chemical properties of the amino acid residues in their structure. This means that some strains among the natural variants of tick-borne encephalitis virus can potentially escape the immune response induced by the standard vaccine.
- MeSH
- aminokyseliny chemie MeSH
- antigeny virové chemie imunologie metabolismus MeSH
- chemické jevy MeSH
- proteiny virového obalu chemie imunologie metabolismus MeSH
- protilátky virové metabolismus MeSH
- vazba proteinů MeSH
- viry klíšťové encefalitidy chemie imunologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Tick-borne encephalitis virus (TBEV) is the causative agent of severe human neuroinfections that most commonly occur after a tick bite. N-Glycosylation of the TBEV envelope (E) glycoprotein is critical for virus egress in mammalian cells, but not in tick cells. In addition, glycans have been reported to mask specific antigenic sites from recognition by neutralizing antibodies. In this regard, the main purpose of our study was to investigate the profile of N-glycans linked to the E protein of TBEV when grown in human neuronal cells and compare it to the profile of virus grown in tick cells. Mass spectrometric analysis revealed significant differences in these profiles. High-mannose glycan with five mannose residues (Man5GlcNAc2), a complex biantennary galactosylated structure with core fucose (Gal2GlcNAc2Man3GlcNAc2Fuc), and a group of hybrid glycans with the composition Gal0-1GlcNAc1Man3-5GlcNAc2Fuc0-1 were confirmed as the main asparagine-linked oligosaccharides on the surface of TBEV derived from human neuronal cells. The observed pattern was supported by examination of the glycopeptides, providing additional information about the glycosylation site in the E protein. In contrast, the profile of TBEV grown in tick cells showed that paucimannose (Man3-4 GlcNAc2Fuc0-1) and high-mannose structures with five and six mannoses (Man5-6GlcNAc2) were major glycans on the viral surface. The reported results complement existing crystallography and cryoelectron tomography data on the E protein structure and could be instrumental for designing carbohydrate-binding antiviral agents active against TBEV.
- MeSH
- glykoproteiny chemie metabolismus MeSH
- glykosylace MeSH
- klíšťata virologie MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- proteiny virového obalu chemie metabolismus MeSH
- sekvence aminokyselin MeSH
- viry klíšťové encefalitidy růst a vývoj metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- antigeny virové analýza MeSH
- buněčné linie patologie MeSH
- lidé MeSH
- proteiny virového obalu analýza imunologie klasifikace MeSH
- protilátky virové analýza MeSH
- spalničky epidemiologie etiologie MeSH
- virové vakcíny epidemiologie imunologie MeSH
- virus spalniček genetika klasifikace růst a vývoj MeSH
- Check Tag
- lidé MeSH
