• MeSH
• acyltransferasy MeSH
• cholesterol analýza   krev   MeSH
• organická chemie MeSH

• MeSH
• acyltransferasy analýza   MeSH
• cholesterol krev   metabolismus   MeSH
• lidé MeSH
• organická chemie MeSH
• svaly analýza   metabolismus   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH

• MeSH
• acyltransferasy MeSH
• cholesterol analýza   krev   metabolismus   MeSH
• klinické enzymatické testy MeSH
• nízká teplota MeSH
• organická chemie MeSH

• MeSH
• cholesterol analýza   krev   MeSH
• hypertenze krev   metabolismus   MeSH
• lidé středního věku MeSH
• organická chemie MeSH
• Check Tag
• lidé středního věku MeSH

• MeSH
• esterifikace MeSH
• játra fyziologie   MeSH
• ketolátky biosyntéza   MeSH
• mastné kyseliny MeSH

OBJECTIVE: To investigate the mechanism by which fat-specific transgenic expression of resistin affects fatty acid metabolism in the spontaneously hypertensive rat (SHR). DESIGN: Basal- and adrenaline-stimulated lipolysis, basal- and insulin-stimulated lipogenesis as well as the site (glycerol versus acyl moiety) of glucose incorporated into triglycerides were determined in adipose tissue isolated from SHR-Resistin transgenic and SHR control rats. RESULTS: A moderate expression of transgenic resistin in adipose tissue was associated with significant increase in the FFA/glycerol ratio during adrenaline-stimulated lipolysis in the SHR-Resistin transgenic rats (3.27+/-0.26) compared to SHR controls (2.11+/-0.10, P=0.0005). Transgenic SHR also exhibited a significant decrease in FFA re-esterification in adipose tissue (approximately by 23%). CONCLUSION: These findings raise the possibility that the prodiabetic effects of transgenic resistin may be partly mediated by increased FFA release from adipose tissue due to impaired FFA re-esterification in adipocytes.

• MeSH
• adrenalin farmakologie   MeSH
• esterifikace MeSH
• geneticky modifikovaná zvířata MeSH
• glukosa metabolismus   MeSH
• glycerol metabolismus   MeSH
• krysa rodu rattus MeSH
• kyseliny mastné neesterifikované * metabolismus   MeSH
• lipolýza fyziologie   účinky léků   MeSH
• potkani inbrední SHR MeSH
• resistin fyziologie   genetika   metabolismus   MeSH
• tuková tkáň * metabolismus   MeSH
• tukové buňky metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH

• MeSH
• esterifikace MeSH
• estery cholesterolu MeSH
• finanční podpora výzkumu jako téma MeSH
• kardiovaskulární nemoci MeSH
• krevní plazma chemie   MeSH
• lidé MeSH
• lipoproteiny HDL krev   MeSH
• lipoproteiny LDL krev   MeSH
• transplantace ledvin škodlivé účinky   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

We examined the association between rate of cholesterol esterification in plasma depleted of apolipoprotein B-containing lipoproteins (FER(HDL)), atherogenic index of plasma (AIP) [(log (TG/HDL-C)], concentrations, and size of lipoproteins and changes in coronary artery stenosis in participants in the HDL-Atherosclerosis Treatment Study. A total of 160 patients was treated with simvastatin (S), niacin (N), antioxidants (A) and placebo (P) in four regimens. FER(HDL) was measured using a radioassay; the size and concentration of lipoprotein subclasses were determined by nuclear magnetic resonance spectroscopy. The S+N and S+N+A therapy decreased AIP and FER(HDL), reduced total VLDL (mostly the large and medium size particles), decreased total LDL particles (mostly the small size), and increased total HDL particles (mostly the large size). FER(HDL) and AIP correlated negatively with particle sizes of HDL and LDL, positively with VLDL particle size, and closely with each other (r = 0.729). Changes in the proportions of small and large lipoprotein particles, which were reflected by FER(HDL) and AIP, corresponded with findings on coronary angiography. Logistic regression analysis of the changes in the coronary stenosis showed that probability of progression was best explained by FER(HDL) (P = 0.005). FER(HDL) and AIP reflect the actual composition of the lipoprotein spectrum and thus predict both the cardiovascular risk and effectiveness of therapy. AIP is already available for use in clinical practice as it can be readily calculated from the routine lipid profile.

• MeSH
• antioxidancia terapeutické užití   MeSH
• apolipoproteiny B metabolismus   MeSH
• ateroskleróza krev   farmakoterapie   metabolismus   radiografie   MeSH
• cholesterol krev   metabolismus   MeSH
• esterifikace MeSH
• fixní kombinace léků MeSH
• koronární angiografie MeSH
• koronární stenóza komplikace   farmakoterapie   MeSH
• lidé MeSH
• lipoproteiny krev   chemie   MeSH
• niacin terapeutické užití   MeSH
• simvastatin terapeutické užití   MeSH
• velikost částic MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH

Tall fescue (Festuca arundinacea Schreb) shows remarkable tolerance to lead (Pb), but the mechanisms involved in metal tolerance are not yet well understood. Here, tall fescue were firstly cultivated hydroponically with Pb2+ (0, 50, 200 and 1000 mg/L) for 14 days. The results showed that remodeling of root architecture plays important roles in tolerance of tall fescue to Pb2+ stress. Increased cell wall (CW) components contribute to restrict high amount of Pb2+ in roots. Additionally, the uronic acid contents of pectin, hemicellulose 1 (HC1) and hemicellulose 2 (HC2) increased under Pb2+ stress. We further observed that tall fescue cultivated with H2O2 showed similar remodeling of root architecture as Pb2+ treatment. Furthermore, pectin, HC1 and HC2 fractions were sequentially extracted from 0 and 10 mM H2O2 treated roots, and Pb2+ adsorption capacity and contents of carboxyl groups of pectin and HC2 fractions were steadily increased under H2O2 treatment in vitro. Our results suggest that degrees of esterification of pectin and HC2 are regulated by H2O2. High amount of low-esterified pectin and HC2 offer more carboxyl groups, provide more Pb2+ binding sites, and restrict more Pb2+ in the CW, which may enhance tolerance of tall fescue to Pb2+ stress.

• MeSH
• buněčná stěna účinky léků   metabolismus   MeSH
• esterifikace MeSH
• Festuca účinky léků   metabolismus   MeSH
• kořeny rostlin účinky léků   metabolismus   MeSH
• látky znečišťující půdu metabolismus   toxicita   MeSH
• olovo metabolismus   toxicita   MeSH
• oxidační stres účinky léků   MeSH
• polysacharidy chemie   metabolismus   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Klíčová slova
• LIPANOR (SANOFI),
• MeSH
• ateroskleróza diagnóza   MeSH
• dítě MeSH
• dospělí MeSH
• finanční podpora výzkumu jako téma MeSH
• hodnocení rizik MeSH
• hyperlipidemie farmakoterapie   MeSH
• krevní plazma analýza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lipoproteiny fyziologie   krev   MeSH
• senioři MeSH
• triglyceridy fyziologie   krev   MeSH
• velikost částic MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• přehledy MeSH
• srovnávací studie MeSH