nanofabrication Dotaz Zobrazit nápovědu
This review discusses the methods of the nanofabrication of metal-polymer composites, especially those based on silver nanostructures. The advantages of composites based on biocompatible polymer matrix, as well as their modifications with suitable techniques are broadly discussed. Different forms of silver nanostructures are reviewed with special emphasis on their antimicrobial activity. Mechanisms of the antimicrobial action of the silver nanostructures are summarized together with proposed ways of passing through the cell wall and processes triggered in cellular environment.
The digital polymerase chain reaction (dPCR) multiplexing method can simultaneously detect and quantify closely related deoxyribonucleic acid sequences in complex mixtures. The dPCR concept is continuously improved by the development of microfluidics and micro- and nanofabrication, and different complex techniques are introduced. In this review, we introduce dPCR techniques based on sample compartmentalization, droplet- and chip-based systems, and their combinations. We then discuss dPCR multiplexing methods in both laboratory research settings and advanced or routine clinical applications. We focus on their strengths and weaknesses with regard to the character of biological samples and to the required precision of such analysis, as well as showing recently published work based on those methods. Finally, we envisage possible future achievements in this field.
- MeSH
- biosenzitivní techniky * MeSH
- polymerázová řetězová reakce MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Refractometric sensors utilizing surface plasmon resonance (SPR) should satisfy a series of performance metrics, bulk sensitivity, thin-film sensitivity, refractive-index resolution, and high-Q-factor resonance, as well as practical requirements such as manufacturability and the ability to separate optical and fluidic paths via reflection-mode sensing. While many geometries such as nanohole, nanoslit, and nanoparticles have been employed, it is nontrivial to engineer nanostructures to satisfy all of the aforementioned requirements. We combine gold nanohole arrays with a water-index-matched Cytop film to demonstrate reflection-mode, high-Q-factor (Qexp = 143) symmetric plasmonic sensor architecture. Using template stripping with a Cytop film, we can replicate a large number of index-symmetric nanohole arrays, which support sharp plasmonic resonances that can be probed by light reflected from their backside with a high extinction amplitude. The reflection geometry separates the optical and microfluidic paths without sacrificing sensor performance as is the case of standard (index-asymmetric) nanohole arrays. Furthermore, plasmon hybridization caused by the array refractive-index symmetry enables dual-mode detection that allows distinction of refractive-index changes occurring at different distances from the surface, making it possible to identify SPR response from differently sized particles or to distinguish binding events near the surface from bulk index changes. Due to the unique combination of a dual-mode reflection-configuration sensing, high-Q plasmonic modes, and template-stripping nanofabrication, this platform can extend the utility of nanohole SPR for sensing applications involving biomolecules, polymers, nanovesicles, and biomembranes.
- MeSH
- biosenzitivní techniky metody MeSH
- fosfatidylcholiny chemie MeSH
- limita detekce MeSH
- liposomy analýza chemie MeSH
- nanopóry * MeSH
- oxid hlinitý chemie MeSH
- povrchová plasmonová rezonance metody MeSH
- sérový albumin hovězí analýza MeSH
- skot MeSH
- zlato chemie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Laboratory respirators are equipped with a set of filters for cleaning the breathing gas. Current filters are mechanical, which are based on the particle/pore size ratio. No abiotic substance avoiding pore obstruction with microbes when the particles are retained in filter is present. A multilayer nanofabric with Ag and Cu ions guarantees fast elimination of microbes trapped in filter thus allowing perfect and reliable filtration. The nanofabric can be tailormade to fit in most filters independently of the breathing gas used.
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab. ; 31 cm
Leg ulcer represents very often therapeutically difficult situation. In the present proposal we will investigate an effect of autodisinfecting properties of polymerized nanofabrics colonized by molecules of nonpolar porphyrin photosensitizer used as a wound dressing. This hypothesis, which was tested in pilot experiments, will be evaluated in a statistically significant group. At the same time an effect of this newly introduced method on the quality of life will be assessed by using the DLQI questionnaires.
Bércové vředy představují často terapeuticky obtížně řešitelnou situaci. V navrženém grantu budeme sledovat vliv autodesinfikujících vlastností polymerní nanotkaniny dopované molekulami nepolárního porfyrinového fotosenzibilizátoru jako krycího materiálu. Tuto hypotézu, kterou jsme již v pilotních studiích demonstrovali, chceme prověřit na statisticky vyhodnotitelné skupině. Zároveň bude posuzován vliv tohoto nového postupu na kvalitu života pacientů pomocí dotazníků DLQI.
- MeSH
- antiinfekční látky lokální MeSH
- bércové vředy terapie MeSH
- chronická nemoc ošetřování MeSH
- fotochemoterapie metody využití MeSH
- fotosenzibilizující látky MeSH
- hojení ran MeSH
- nanovlákna MeSH
- obvazy MeSH
- porfyriny MeSH
- žilní insuficience komplikace MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- angiologie
- ošetřovatelství
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
Anastomotic leakage is a severe complication in gastrointestinal surgery. It is often a reason for reoperation together with intestinal passage blockage due to formation of peritoneal adhesions. Different materials as local prevention of these complications have been studied, none of which are nowadays routinely used in clinical practice. Nanofabrics created proved to promote healing with their structure similar to extracellular matrix. We decided to study their impact on anastomotic healing and formation of peritoneal adhesions. We performed an experiment on 24 piglets. We constructed 3 hand sutured end-to-end anastomoses on the small intestine of each pig. We covered the anastomoses with a sheet of polycaprolactone nanomaterial in the first experimental group, with a sheet of copolymer of polylactic acid with polycaprolactone in the second one and no fortifying material was used in the Control group. The animals were sacrificed after 3 weeks of observation. Clinical, biochemical and macroscopic signs of anastomotic leakage or intestinal obstruction were monitored, the quality of the scar tissue was assessed histologically, and a newly developed scoring system was employed to evaluate the presence of adhesions. The material is easy to manipulate with. There was no mortality or major morbidity in our groups. No statistical difference was found inbetween the groups in the matter of level of peritoneal adhesions or the quality of the anastomoses. We created a new adhesion scoring system. The material appears to be safe however needs to be studied further to prove its' positive effects.
- MeSH
- adheze tkání etiologie prevence a kontrola MeSH
- anastomóza chirurgická MeSH
- duodenum chirurgie MeSH
- hojení ran MeSH
- mikroskopie elektronová rastrovací MeSH
- modely nemocí na zvířatech MeSH
- náhodné rozdělení MeSH
- nanovlákna terapeutické užití ultrastruktura MeSH
- nemoci peritonea etiologie prevence a kontrola MeSH
- netěsnost anastomózy prevence a kontrola MeSH
- polyestery MeSH
- prasata MeSH
- testování materiálů MeSH
- tkáňové podpůrné struktury * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
