paratuberculosis
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- MeSH
- Crohnova nemoc diagnóza patologie MeSH
- dospělí MeSH
- finanční podpora výzkumu jako téma MeSH
- intracelulární infekce bakterií Mycobacterium avium diagnóza patologie MeSH
- lidé MeSH
- mikrobiologické techniky metody MeSH
- Mycobacterium avium subsp. paratuberculosis patogenita MeSH
- paratuberkulóza diagnóza patologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
Úvod: Etiologie Crohnovy choroby zůstává neobjasněná. Význam infekce Mycobacterium avium subsp. paratuberculosis (MAP) pro vznik Crohnovy choroby byl dlouhodobě ovlivněn jeho obtížnou kultivací. Cílem studie bylo určit výskyt MAP pomocí vysoce citlivé metody PCR (hsPCR) pro průkaz specifického úseku DNA IS900 a posoudit na základě výsledků i literárních údajů význam MAP v etiologii Crohnovy choroby. Materiál a metodika: U 106 pacientů operovaných pro Crohnovu chorobu bylo v resekátech provedeno vyšetření na MAP metodou hsPCR. Resekce terminálního ilea a céka - primooperace - byla provedena u 61 pacientů, reoperace u 32 pacientů, dominantní operace tlustého střeva u 11 pacientů, resekce jejuna u 2 pacientů. Výsledky ze vzorků z postižené tkáně byly srovnány s výsledky z makroskopicky nepoškozené tkáně (kontrolní vzorky). Výsledky: V postižené tkáni bylo MAP prokázáno u 58 pacientů (54,7 %). U 14 z nich (13,2 %) bylo MAP prokázáno i v kontrolních vzorcích. U 4 pacientů (3,8 %) bylo MAP zjištěno jenom v nepoškozené tkáni. Celkem bylo MAP prokázáno u 62 pacientů (58,5 %). Závěr: Významně vyšší výskyt MAP u pacientů s Crohnovou chorobou v oblasti střevního postižení ve srovnání s výskytem v oblasti nepostiženého střeva podporuje úvahy o možné účasti MAP v etiologii Crohnovy choroby.
Introduction: The etiology of Crohn's disease remains open. The significance of Mycobacterium avium subsp. paratuberculosis (MAP) in the development of Crohn's Disease has for a long time been influenced by the great difficulties associated with the cultivation procedure. The goal of our study was to determine the incidence of MAP by the new highly sensitive PCR method (hsPCR) for the detection of specific DNA fragment IS900 and together with reports in the literature, to assess the position of MAP in the etiology of Crohn's disease. Material and methods: Small specimens taken from the operated gut of 106 patients with Crohn's disease were examined for the presence of MAP by a highly sensitive PCR method. Resection of terminal ileum and cecum -primary operations were performed in 61 patients, reoperation in 32 patients, dominant operation of the colon in 11 patients, resection of the jejunum in 2 patients. The results from the involved pathological tissue were compared with the results from macroscopically unaffected tissue (controls). Results: In affected tissue, MAP was present in 58 patients (54.7 %). In 14 of these patients (13.2 %), MAP was also found in control specimens. In 4 patients (3.8 %) MAP was found only in unaffected tissue. In total, MAP was found in 62 patients (58.5 %). Conclusion: A significantly higher incidence of MAP found in the affected tissue of patients with Crohn's disease in comparison with unaffected tissue supports the view of the possible role of MAP in the etiology of Crohn's disease.
- MeSH
- Crohnova nemoc mikrobiologie MeSH
- Haplorrhini MeSH
- lidé MeSH
- Mycobacterium patogenita MeSH
- paratuberkulóza veterinární MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : tab. ; 32 cm
Význam M.paratuberculosis v etiopatogenezi Crohnovy choroby bude studován průkazem specifického fragmentu IS900 v archivovaných parafinových bločcích a nativní střevní tkáni pacientů s Crohnovou chorobou,v mléku a mléčných výrobcích. Izolované kmeny M.paratuberculosis budou blíže identifikovány metodami molekulární biologie a srovnány s kmeny izolovanými v jiných laboratořích ve světě.; The role of M.pyprytuberculosis in the etiopathogenesis of Crohn s disease will be studiend using test for proof with specific fragment IS900 in stored parrafin embedded blocks and native tissue from patients with Crohn s disease. Further in milk and milk products and other biologic material. Isolated stems of M.paratuberculosis will be in detail identified using methods of molecular biology and the results will be compared with those form other laboratories in the world.
- MeSH
- Crohnova nemoc MeSH
- Mycobacterium avium subsp. paratuberculosis MeSH
- paratuberkulóza MeSH
- polymerázová řetězová reakce využití metody MeSH
- Geografické názvy
- Česká republika MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- gastroenterologie
- bakteriologie
- infekční lékařství
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
Paratuberculosis, a chronic disease affecting ruminant livestock, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). It has direct and indirect economic costs, impacts animal welfare and arouses public health concerns. In a survey of 48 countries we found paratuberculosis to be very common in livestock. In about half the countries more than 20% of herds and flocks were infected with MAP. Most countries had large ruminant populations (millions), several types of farmed ruminants, multiple husbandry systems and tens of thousands of individual farms, creating challenges for disease control. In addition, numerous species of free-living wildlife were infected. Paratuberculosis was notifiable in most countries, but formal control programs were present in only 22 countries. Generally, these were the more highly developed countries with advanced veterinary services. Of the countries without a formal control program for paratuberculosis, 76% were in South and Central America, Asia and Africa while 20% were in Europe. Control programs were justified most commonly on animal health grounds, but protecting market access and public health were other factors. Prevalence reduction was the major objective in most countries, but Norway and Sweden aimed to eradicate the disease, so surveillance and response were their major objectives. Government funding was involved in about two thirds of countries, but operations tended to be funded by farmers and their organizations and not by government alone. The majority of countries (60%) had voluntary control programs. Generally, programs were supported by incentives for joining, financial compensation and/or penalties for non-participation. Performance indicators, structure, leadership, practices and tools used in control programs are also presented. Securing funding for long-term control activities was a widespread problem. Control programs were reported to be successful in 16 (73%) of the 22 countries. Recommendations are made for future control programs, including a primary goal of establishing an international code for paratuberculosis, leading to universal acknowledgment of the principles and methods of control in relation to endemic and transboundary disease. An holistic approach across all ruminant livestock industries and long-term commitment is required for control of paratuberculosis.
- MeSH
- chov zvířat MeSH
- divoká zvířata mikrobiologie MeSH
- hlášení nemocí normy MeSH
- incidence MeSH
- Mycobacterium avium subsp. paratuberculosis izolace a purifikace MeSH
- paratuberkulóza ekonomika epidemiologie prevence a kontrola MeSH
- přežvýkavci mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Johne's disease (paratuberculosis) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) and can lead to severe economic losses in the affected cattle herds. The transmission of the disease occurs mainly orally, by the ingestion of MAP, which is shed in the feces and milk of infected animals. Calves show a high susceptibility for the infection compared to adult animals. The use of milk replacers can, therefore, contribute to the prevention of the transmission of the disease to calves in MAP-positive herds by preventing the ingestion of the bacterium with milk from infected animals. The objective of this study was to test milk replacers for calves for the presence of MAP by bacteriological culture and PCR. Therefore, commercially available milk replacers for calves were purchased from 15 different companies. All of the products were tested for MAP by solid culture and real time quantitative PCR (qPCR) targeting IS900 and F57. During the present study, MAP could not be detected by qPCR or solid culture in commercially available milk replacers for calf rearing. The results of the present study underpins that the use of milk replacers for calf rearing might contribute to the reduction of MAP intake by calves in JD positive herds. Additional studies, including more products with a higher diversity, are needed to further elucidate the presence or absence of MAP in milk replacers for calves.
- MeSH
- feces mikrobiologie MeSH
- krmivo pro zvířata mikrobiologie MeSH
- Mycobacterium avium subsp. paratuberculosis izolace a purifikace MeSH
- nemoci skotu mikrobiologie MeSH
- paratuberkulóza mikrobiologie MeSH
- polymerázová řetězová reakce metody veterinární MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
In this study, products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected dairy cattle were examined and quantified for the presence of the causal agent of paratuberculosis, Mycobacterium avium subsp. paratuberculosis, using culture and quantitative real-time PCR (qPCR). Viable M. avium subsp. paratuberculosis cells were detected using culture in fermentors for up to 2 months; the presence of M. avium subsp. paratuberculosis DNA (10(1) cells/g) was demonstrated in all anaerobic fermentors and digestate 16 months after initiation of work at a biogas plant, using IS900 qPCR. F57 qPCR was able to detect M. avium subsp. paratuberculosis DNA (10(2) cells/g) at up to 12 months. According to these results, a fermentation process that extended beyond 2 months removed all viable M. avium subsp. paratuberculosis cells and therefore rendered its product M. avium subsp. paratuberculosis free. However, M. avium subsp. paratuberculosis DNA was found during all the examined periods (more than 1 year), which could be explained by either residual DNA being released from dead cells or by the presence of viable cells whose amount was under the limit of cultivability. As the latter hypothesis cannot be excluded, the safety of the final products of digestion used for fertilization or animal bedding cannot be defined, and further investigation is necessary to confirm or refute this risk.
- MeSH
- anaerobióza MeSH
- bakteriální nálož metody MeSH
- biopaliva MeSH
- bioreaktory mikrobiologie MeSH
- časové faktory MeSH
- DNA bakterií genetika izolace a purifikace MeSH
- hnůj mikrobiologie MeSH
- Mycobacterium avium subsp. paratuberculosis genetika růst a vývoj izolace a purifikace MeSH
- nemoci skotu mikrobiologie MeSH
- paratuberkulóza mikrobiologie MeSH
- polymerázová řetězová reakce metody MeSH
- skot MeSH
- transpozibilní elementy DNA MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.
- MeSH
- DNA bakterií genetika MeSH
- multiplexová polymerázová řetězová reakce MeSH
- Mycobacterium avium subsp. paratuberculosis klasifikace genetika izolace a purifikace MeSH
- nemoci ovcí diagnóza mikrobiologie MeSH
- ovce MeSH
- paratuberkulóza diagnóza mikrobiologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- transpozibilní elementy DNA MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Argentina MeSH
The aims of this study were to describe spatial contamination of the environment on a mouflon pasture, as well as to assess the contamination of grass and roots after surface contamination and in depth contamination with feces and buried tissues from animals infected with Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis). Samples of soil, roots, and aerial parts of plants were collected from different locations inside the mouflon pasture, and one control sample site was chosen outside the area where the animals are living. M. a. paratuberculosis DNA was present in all the examined sites and was more often detected in roots than in soil. DNA was detected at up to 80 cm of depth and was spatially more widespread than the initial hypothesis of M. a. paratuberculosis leaching vertically into deeper layers of soil. This study broadens our knowledge of the spread and persistence of M. a. paratuberculosis in an environment with highly infected animals.
- MeSH
- krmivo pro zvířata analýza mikrobiologie MeSH
- lipnicovité mikrobiologie MeSH
- Mycobacterium avium subsp. paratuberculosis genetika izolace a purifikace fyziologie MeSH
- nemoci skotu mikrobiologie přenos MeSH
- paratuberkulóza mikrobiologie přenos MeSH
- půda chemie MeSH
- půdní mikrobiologie * MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
