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Acta paediatrica ; Vol. 83 Supplement 397
[1st ed.] 102 s. : obr., tab., grafy ; 28 cm
- MeSH
- celková anestezie MeSH
- dítě MeSH
- intratracheální intubace metody MeSH
- ketamin aplikace a dávkování MeSH
- popálení chirurgie MeSH
- Check Tag
- dítě MeSH
- Publikační typ
- kazuistiky MeSH
The epithelial cells of choroid plexus (CP) in brain ventricles produce cerebrospinal fluid and act as the blood-cerebrospinal fluid barrier. In this study, we confirmed that CP in the 4th ventricle is composed of cellular oscillators that all harbor glucocorticoid receptors and are mutually synchronized to produce a robust clock gene expression rhythm detectable at the tissue level in vivo and in vitro. Animals lacking glucocorticoids (GCs) due to surgical removal of adrenal glands had Per1, Per2, Nr1d1 and Bmal1 clock gene rhythmicity in their CP significantly dampened, whereas subjecting them to daily bouts of synthetic GC analog, dexamethasone (DEX), reinforced those rhythms. We verified these in vivo effects using an in vitro model of organotypic CP explants; depending on the time of its application, DEX significantly increased the amplitude and efficiently reset the phase of the CP clock. The results are the first description of a PRC for a non-neuronal clock in the brain, demonstrating that CP clock shares some properties with the non-neuronal clocks elsewhere in the body. Finally, we found that DEX exhibited multiple synergic effects on the CP clock, including acute activation of Per1 expression and change of PER2 protein turnover rate. The DEX-induced shifts of the CP clock were partially mediated via PKA-ERK1/2 pathway. The results provide the first evidence that the GC rhythm strengthens and entrains the clock in the CP helping thus fine-tune the brain environment according to time of day.
- MeSH
- cirkadiánní hodiny * MeSH
- cirkadiánní proteiny Period genetika metabolismus MeSH
- dexamethason MeSH
- glukokortikoidy metabolismus MeSH
- MAP kinasový signální systém MeSH
- nadledviny metabolismus MeSH
- nucleus suprachiasmaticus metabolismus MeSH
- plexus chorioideus metabolismus MeSH
- potkani Wistar MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- lidé MeSH
- nemoci páteře chirurgie MeSH
- poruchy dýchání komplikace MeSH
- Check Tag
- lidé MeSH
