Předčasná ejakulace je jednou z běžných sexuálních poruch mužů. V naší studii jsme se zaměřili na předčasnou ejakulaci jako možný problém celého sexuálního páru. Hodnotili jsme možný vliv předčasné ejakulace mužů na vznik sexuální dysfunkce u jejich partnerek a následné odstranění této ženské dysfunkce pouze léčbou mužů. Z 95 párů bylo do pilotní studie zařazeno 50 párů, které splnily daná kritéria na základě diagnostických nástrojů. U mužů byla potvrzena předčasná ejakulace, u žen sexuální dysfunkce, jejímž zdrojem byla pouze předčasná ejakulace. Výsledky škály Arizona Sexual Experience Scale-Male a Female vykazují Spearmanovu korelaci před léčbou (R = 0,68) a po 6 měsících léčby (R = 0,90). Dále jsme potvrdili vztah mezi Arizona Sexual Experience Scale-Female a Female Sexual Function Index před léčbou (R = 0,42) a po léčbě (R = 0,59). Všechna data byla potvrzena Mannovým-Whitneyovým testem. Výsledky naší pilotní studie poukazují na málo prozkoumaný fakt, že předčasná ejakulace není pouze problémem mužů, ale může se stát následným sexuálním problémem celého páru. Výsledky této pilotní studie představují důležité zjištění o pozitivním účinku léčby mužů s předčasnou ejakulací na následnou sexuální dysfunkci jejich sexuálních partnerek.

Premature ejaculation is one of the common sexual disorders of men. In our study we focused on premature ejaculation as a possible problem for the entire sexual couple. We evaluated the possible effect of men premature ejaculation on the development of sexual dysfunction in their wives and the subsequent elimination of this female dysfunction if only men are treated. Out of 95 couples, 50 couples who met the given criteria based on the diagnostic tools were included in the pilot study. In men, premature ejaculation was confirmed, in women, sexual dysfunction, the source of which was only premature ejaculation. Results of the Arizona Sexual Experience Scale-Male and Female relationship are characterized by Spearman’s correlation before treatment (R = 0.68), after 6 months of treatment (R = 0.90). Furthermore, we confirmed the relationship between Arizona Sexual Experience Scale-Female and Female Sexual Function Index before treatment (R = 0.42) and after treatment (R = 0.59). All data were confirmed by the Mann-Whitney test. The results of our pilot study point to the little-explored fact that premature ejaculation is not only a problem for men, but can also form a subsequent sexual problem for the couple as a whole. The results of this pilot study represent important findings regarding the positive effect of treatment of men with premature ejaculation on the resulting sexual dysfunction in their sexual partners.

• MeSH
• lidé MeSH
• paroxetin aplikace a dávkování   terapeutické užití   MeSH
• pilotní projekty MeSH
• předčasná ejakulace * diagnóza   farmakoterapie   MeSH
• průzkumy a dotazníky MeSH
• selektivní inhibitory zpětného vychytávání serotoninu MeSH
• sexuální dysfunkce fyziologická diagnóza   MeSH
• sexuální dysfunkce psychické diagnóza   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

Endometrióza je chronické, estrogen-dependentní, zánětlivé onemocnění, jehož podstatou je růst endometriotické tkáně v mimoděložních lokalitách. Z široké škály klinické manifestace endometriózy jsou chronická pánevní bolest, dysmenorea, dyspareunie a subfertilita hlavní symptomy signifikantně snižující kvalitu života postižených žen. I přes skutečnost, že je endometrióza považována za benigní onemocnění, sdílí některé rysy typické pro maligní nádory. Jedním z nich je abnormální morfologie, v rámci které mohou být v ložiscích endometriózy přítomny atypie žlázového epitelu nevykazující známky hyperplazie nebo se může jednat o glandulární hyperplazií, kterou mohou, ale nemusejí doprovázet buněčné atypie. Tuto situaci reflektuje termín atypická endometrióza, jejíž diagnostické zařazení není snadné. Buněčné atypie těžkého stupně mohou totiž být reaktivního původu, a tak je vzájemné odlišení dysplastických a reaktivních změn ve tkáni endometriózy limitované a problematické. Naše pracovní skupina z vědeckého centra pro léčbu endometriózy Nemocnice Znojmo se v nedávné době atypickou endometriózou zabývala a poukázala na potenciální využití imunohistochemie v její diagnostice. Za použití jednoduchého imunohistochemického panelu s protilátkami proti estrogenovým receptorům, progesteronovým receptorům a nádorovému supresoru p53 jsme ve tkáni atypické endometriózy zjistili výrazně nižší míru exprese hormonálních receptorů a nárůst exprese p53 ve srovnání s normální (typickou) endometriózou. Vzhledem k nízkému počtu analyzovaných případů a nekonzistentním výsledkům studií zabývajících se hormonálními receptory (i jinými markery) v atypické endometrióze musí být námi popsaný imunohistochemický panel ověřen na větším počtu případů. V rutinní histopatologické praxi není atypická endometrióza zažitou jednotkou. Je však důležité se s ní seznámit, neboť její přítomnost je spojena s častějším výskytem určité skupiny nádorů označovaných jako nádory asociované s endometriózou, zejména pak s endometroidním karcinomem a světlobuněčným karcinomem.

Endometriosis is a chronic, estrogen-dependent, inflammatory disease characterized by the growth of endometriotic tissue outside the uterus. Among the wide spectrum of clinical manifestations of endometriosis, chronic pelvic pain, dysmenorrhea, dyspareunia and subfertility are the main symptoms that significantly reduce the quality of life of affected women. Despite the fact that endometriosis is considered a benign disease, it shares some features typical of malignant tumors. One of them is abnormal morphology, which indicates atypia of the glandular epithelium without signs of hyperplasia, or it may be glandular hyperplasia, which may or may not be accompanied by cellular atypia. This situation is reflected in the term atypical endometriosis, the diagnosis of which is not easy. Cellular atypia of a severe degree can be of reactive origin, so the mutual differentiation of dysplastic and reactive changes in endometriotic tissue is limited and problematic. Our working group from the scientific center for the treatment of endometriosis at the Znojmo Hospital recently dealt with atypical endometriosis and pointed out the potential utility of immunohistochemistry in its diagnosing. Using a simple immunohistochemical panel with antibodies against estrogen receptors, progesterone receptors, and the tumor suppressor p53, we found significantly lower levels of hormone receptor expression and increased p53 expression in atypical endometriosis compared to normal (typical) endometriosis. Due to the low number of cases analyzed and the inconsistent results of studies dealing with hormone receptors (and other markers) in atypical endometriosis, the usefulness of the immunohistochemical panel described in our study must be verified on a larger number of cases. In routine histopathological practice, atypical endometriosis is not a well-known entity. However, it is important to become familiar with it because its presence is associated with a higher incidence of a certain group of tumors known as endometriosis-associated cancers, especially endometrioid carcinoma and clear cell carcinoma.

• MeSH
• diagnostické techniky molekulární metody   MeSH
• endometrióza * diagnóza   genetika   klasifikace   komplikace   MeSH
• imunohistochemie * metody   MeSH
• lidé MeSH
• nádorový supresorový protein p53 analýza   genetika   MeSH
• nádory vaječníků diagnóza   etiologie   klasifikace   MeSH
• receptory pro estrogeny analýza   genetika   MeSH
• receptory progesteronu analýza   genetika   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• klinická studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

Extraskeletal myxoid chondrosarcoma (EMC) is a rare sarcoma of uncertain lineage. Insulinoma-associated protein 1 (INSM1) has recently been described as a highly specific and sensitive immunohistochemical marker for EMC. The goal of this study was to evaluate the diagnostic significance of INSM1 immunohistochemistry in EMC. Furthermore, correlations between molecular and morphological findings were performed. Sixteen of 17 EMC cases were stained with the INSM1 antibody. Tumors with at least 5% INSM1-positive cells and any staining intensity were considered positive. Molecular testing was successfully performed in 12/17 cases. The immunohistochemical analysis detected 13 INSM1-positive (81%) and 3 INSM1-negative tumors (19%). The extent of the staining was classified as 1+ in 7 cases (44%), 2+ in 2 cases (13%), 3+ in 2 cases (13%) and 4+ in 2 cases (13%). Intensity of immunostaining was weak in 5 cases (31%), moderate in 2 cases (13%) and strong in 6 cases (38%). Molecular assays revealed 8 EWSR1::NR4A3 positive tumors (67%), 2 TAF15::NR4A3 positive tumors (17%), 1 TCF12::NR4A3 positive tumor (8%) and 1 NR4A3 positive tumor (8%) in which no other gene alteration was identified. Two of them, namely TCF12 positive and one TAF15 positive tumors, were highly cellular and partially associated with pseudopapillary architecture. Our study found that moderate/strong expression of INSM1 in more than 25% of tumor cells was present in only 31% of cases. Thus, the diagnostic utility of INSM1 is rather low. Two morphologically unique cases of non-EWSR1 rearranged EMC with an extremely rare pseudopapillary growth pattern are also reported.

• MeSH
• chondrosarkom * diagnóza   genetika   MeSH
• DNA vazebné proteiny genetika   MeSH
• fúzní onkogenní proteiny genetika   metabolismus   MeSH
• lidé MeSH
• nádory z pojivové a měkké tkáně * genetika   MeSH
• receptory thyreoidních hormonů genetika   MeSH
• represorové proteiny genetika   MeSH
• sarkom * genetika   MeSH
• steroidní receptory * genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Sarcoidosis is a disease characterised primarily by lung tissue involvement. Extrapulmonary involvement, particularly in the genitourinary tract, is extremely rare, particularly when it comes to primary disease detection in this location. The gold standard in establishing a definitive diagnosis of sarcoidosis is a combination of the clinical picture, the results of imaging methods, and histopathological examination from the biopsy taken (thus ruling out other causes of granulomatous inflammation). However, it is common for the biopsy to be infeasible or for the patient to refuse such an examination, resulting in the neglect of this critical verification. We introduce the case of a young 29-year-old man of Czech nationality who had been complaining for some time about non-specific pain above the pubic bone and in the lower abdomen, which was combined with a painless enlargement of the right half of the scrotum. Due to suspected malignancy, it was, after considering clinical, imaging, and laboratory findings, decided to perform a radical orchiectomy as a treatment option. The histological examination revealed that it was not cancer, but rather a rare genitourinary form of extrapulmonary sarcoidosis. In this case, radical resection had been, therefore, unnecessary. We also present a review of the literature on published extrapulmonary, genitourinary, and testicular sarcoidosis cases. All the above demonstrates the importance of considering a possible atypical sarcoidosis manifestation and histological confirmation before pursuing radical solutions.

• MeSH
• biopsie MeSH
• dospělí MeSH
• lidé MeSH
• orchiektomie MeSH
• sarkoidóza * diagnóza   chirurgie   patologie   MeSH
• testikulární nádory * diagnóza   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH
• přehledy MeSH

Amyloidosis is a rare metabolic disorder primarily brought on by misfolding of an autologous protein, which causes its local or systemic deposition in an aberrant fibrillar form. It is quite rare for pulmonary tissue to be impacted by amyloidosis; of the three forms it can take when involving pulmonary tissue, nodular pulmonary amyloidosis is the most uncommon. Nodular pulmonary amyloidosis rarely induces clinical symptoms, and most often, it is discovered accidentally during an autopsy or via imaging techniques. Only one case of nodular pulmonary amyloidosis, which manifested as a spontaneous pneumothorax, was found in the literature. In terms of more precise subtyping, nodular amyloidosis is typically AL or mixed AL/AH type. No publications on AH-dominant type of nodular amyloidosis were found in the literature. We present a case of an 81 years-old male with nodular pulmonary AH-dominant type amyloidosis who presented with spontaneous pneumothorax. For a deeper understanding of the subject, this study also provides a review of the literature on cases with nodular pulmonary amyloidosis in relation to precise amyloid fibril subtyping. Since it is often a difficult process, accurate amyloid type identification is rarely accomplished. However, this information is very helpful for identifying the underlying disease process (if any) and outlining the subsequent diagnostic and treatment steps. Even so, it is crucial to be aware of this unit and make sure it is taken into consideration when making a differential diagnosis of pulmonary lesions.

• MeSH
• amyloidóza * komplikace   diagnóza   patologie   MeSH
• lidé MeSH
• plíce diagnostické zobrazování   patologie   MeSH
• plicní nemoci * komplikace   diagnóza   patologie   MeSH
• pneumotorax * diagnóza   etiologie   MeSH
• senioři nad 80 let MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• Publikační typ
• kazuistiky MeSH
• přehledy MeSH

Delayed ejaculation belongs to the group of sexual disorders in men. The causes of delayed ejaculation or anejaculation are not exactly known. It is assumed that it can be caused by psychogenic or organic influences or their combinations. One of the causes of delayed ejaculation may be elevated prolactin levels, which may be increased by psychosocial stress, pituitary disorders or also treatment with selective serotonin reuptake inhibitors in the treatment of depression. We tested a selected group of 50 men who were diagnosed with a depressive disorder and whose antidepressant treatment lasted for at least 24 weeks. These patients reported long-term delayed ejaculation or, in some cases, anejaculation as comorbidity. The results showed significant Spearman's correlation between elevated prolactin levels and intravaginal ejaculation latency values (R = 0.45), as well as between Beck's Depression-II inventory and intravaginal ejaculation latency and latency values (R = 0.48).

• MeSH
• depresivní poruchy * MeSH
• ejakulace MeSH
• lidé MeSH
• předčasná ejakulace * etiologie   MeSH
• prolaktin MeSH
• selektivní inhibitory zpětného vychytávání serotoninu farmakologie   terapeutické užití   MeSH
• sexuální dysfunkce fyziologická * etiologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The aim of this study was to reveal the effects of foodborne fluoxetine on morphological and condition profile, hematological profile, biochemical and oxidative stress indices on juvenile rainbow trout. The study was performed according to OECD Guideline No. 215. Fluoxetine was incorporated into Biomar 921 pellets at a dose of 0.047 mg/kg (environmental concentration), 0.577 mg/kg and 6.7 mg/kg. There was statistically significant change in hematological profile, including an increasing trend in neutrophil/lymphocyte ratio and a decreasing trend in the number of lymphocytes. Measurements of oxidative stress indicated decreased activity of the detoxifying enzyme glutathione-S-transferase in the liver and kidney. However, the measurement of GR, GPx, CAT, SOD activity, and TBARS showed no changes. Histopathological examination revealed damage to the proximal tubules of caudal kidney in exposed groups. This study confirms that fluoxetine has a significant effect on immune response.

• MeSH
• antidepresiva druhé generace toxicita   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• fluoxetin toxicita   MeSH
• imunita účinky léků   MeSH
• kontaminace potravin MeSH
• krevní obraz MeSH
• krmivo pro zvířata MeSH
• Oncorhynchus mykiss krev   imunologie   MeSH
• oxidační stres účinky léků   MeSH
• proximální tubuly ledvin účinky léků   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: Endometrial tissue plays an important role in the regulation of female fertility and there is evidence that endometrial pathology (including endometriosis) is closely related to endocrine disorders. On the other hand, various neuroendocrine changes can be significantly affected by psychosocial stress. In connection with these findings, we tested the relationship between neuroendocrine changes, sexual dysfunction, psychosocial/traumatic stress, and dissociative symptoms in women with endometriosis. METHODS: A total of 65 patients with endometriosis were included in the study. Clinical examinations were focused on the biochemical analysis of neuroendocrine markers of endometriosis (cancer antigen 125 [CA 125] and cancer antigen 19-9 [CA 19-9]), estradiol, psychometric evaluation of sexual dysfunction, psychosocial/traumatic stress, and dissociative symptoms. RESULTS: The results showed significant Spearman correlations between the values of the revised range of sexual difficulties for sexual dysfunction (Revised Female Sexual Distress Scale), psychosocial/traumatic stress (Trauma Symptoms Checklist) (R = 0.31), and dissociative symptoms (Somatoform Dissociation Questionnaire) (R = 0.33). Positive correlations were also found between CA 125 and CA 19-9 (R = 0.63), and between CA 125 and the results of the values of the revised scale of sexual difficulties for sexual dysfunction (Revised Female Sexual Distress Scale) (R = 0.29). Also psychosocial/traumatic stress (Trauma Symptoms Checklist) significantly correlated with CA 125 (R = 0.38) and with CA 19-9 (R = 0.33). CONCLUSION: These results represent the first findings regarding the relationship of the neuroendocrine markers CA 125 and CA 19-9 and sexual dysfunction with trauma/stress-related symptoms and dissociative symptoms in women with endometriosis.

• MeSH
• antigen CA-125 krev   MeSH
• antigen CA-19-9 krev   MeSH
• disociační poruchy diagnóza   psychologie   MeSH
• dospělí MeSH
• endometrióza * krev   komplikace   psychologie   MeSH
• korelace dat MeSH
• lidé MeSH
• neurosekreční systémy metabolismus   MeSH
• psychické trauma * komplikace   diagnóza   patofyziologie   MeSH
• psychologické techniky MeSH
• psychologie MeSH
• sexuální dysfunkce fyziologická * krev   psychologie   MeSH
• somatoformní poruchy * diagnóza   patofyziologie   psychologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Recentní publikace o závažném a častém gynekologickém onemocnění - endometrióze - v českém odborném písemnictví v současnosti chybí. Cílem autorů s dlouholetými zkušenostmi v oboru gynekologie, patologické anatomie a sexuologie bylo podat komplexní a moderní pohled na toto multidisciplinární onemocnění.; Text seznamuje s klinickými příznaky onemocnění a současnými vyšetřovacími metodami, popisuje medikamentózní a chirurgickou léčbu.

• Klíčová slova
• Gynekologie a porodnictví,
• MeSH
• endometrióza MeSH

• NLK Obory
• gynekologie a porodnictví

Primary urethral adenocarcinomas are very rare neoplasms accounting for <10% of all urethral carcinomas. Site of their origin is unclear, but they seem to arise from Skene's paraurethral glands, which is the female homologue of the male prostate. The aim of this article is to report the first case of Skene's gland adenocarcinoma in which a molecular genetic profiling was performed. The patient was a 73-year-old woman with a polypoid lesion sized 3 × 2 cm located at the interface between the bladder neck and the proximal urethra. Transurethral resection was performed and small tissue fragments with positive margins were obtained. Histology revealed an epithelial neoplasm consisting of cribriform structures located in the subepithelial connective tissue of the bladder wall and proximal urethra. The lesion showed positive immunohistochemical staining with prostate specific antigen, prostatic acid phosphatase, NKX3.1, and alpha-methylacyl-CoA racemase. Using the Illumina TruSight Tumor 170 next-generation sequencing assay, a mutation and loss of heterozygosity of the phosphatase and tensin homologue (PTEN) gene was detected. No fusion in any of the examined genes was found using this assay as well as FusionPlex Solid Tumor Kit and FusionPlex Sarcoma kit assays from ArcherDX. Given the rarity of Skene's gland adenocarcinoma, it is uncertain whether the same grading and prognostic criteria that are currently used for prostatic cancer apply here as well. It is also unclear what treatment strategy should be applied, but according to the available literature, it seems that local excision or wide surgical resection could represent sufficient therapeutic modalities.

• MeSH
• adenokarcinom diagnóza   genetika   patologie   MeSH
• fosfohydroláza PTEN genetika   MeSH
• lidé MeSH
• mutace MeSH
• nádorové biomarkery genetika   MeSH
• nádory močové trubice diagnóza   genetika   patologie   MeSH
• senioři MeSH
• uretra patologie   MeSH
• ztráta heterozygozity MeSH
• Check Tag
• lidé MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH