Background: Femoroacetabular impingement syndrome (FAI) is a complex, often post-traumatically developing impairment of the hip joint. It is characterized by ambiguous symptomatology, which makes early diagnosis difficult. Aim: The study was conducted to evaluate the applicability of a triaxial gyroscopic sensor in routine practice as an additional indication criterion for operative versus conservative treatment procedures. Methods: Ninety-two patients were included in the experimental retrospective study and 62 completed the examination. All patients signed informed consent. A gyroscopic sensor was placed on the right side of the pelvis above the hip joint and patients walked approximately 15 steps. Data were also evaluated while the patients climbed stairs. A complete clinical examination of the dynamics and physiological movements in the joint was performed. The data measured by the gyroscopic sensor were processed using differential geometry methods and subsequently evaluated using spectral analysis and neural networks. Results: FAI diagnosis using gyroscopic measurement is fast and easy to implement. Our approach to processing the gyroscopic signals used to detect the stage of osteoarthritis and post-traumatic FAI could lead to more accurate detection and capture early in FAI development. Conclusions: The obtained data are easily evaluated, interpretable, and beneficial in the diagnosis of the early stages of FAI. The results of the study show that this approach can lead to more accurate and early detection of osteoarthritis and post-traumatic FAI.
- MeSH
- analýza chůze metody MeSH
- biomechanika * MeSH
- femoroacetabulární impingement * chirurgie diagnóza patofyziologie MeSH
- kyčelní kloub patofyziologie MeSH
- lidé MeSH
- nositelná elektronika * MeSH
- osteoartróza diagnóza patofyziologie MeSH
- retrospektivní studie MeSH
- telemedicína metody MeSH
- umělá inteligence MeSH
- Check Tag
- lidé MeSH
Background: Lipocalin-2 (LCN2) is a protein that has been associated with skeletal muscle regeneration, but details regarding its role in Arthritis remain unclear. The aim of the current study was to investigate LCN2 levels of Arthritis patients and its relationship with oxidative and antioxidative factors.Methods: The study includes (125) blood samples of persons aged 20-65 years were divided into a control group (apparently healthy) consisting of 55 samples [31female, 24 males] and a Patient group consisting of 70 samples [37female, 33 males] who were attending the bone diseases consultation unit at the Ibn Sina Teaching Hospital in Mosul, Iraq. Venous blood samples (10 ml) were collected after overnight fasting. To conduct Clinical analyses: Serum LCN2 level was determined by ELISA, also Malonaldehyde, glutathione, vitamin E, vitamin C, peroxy nitrite, peroxidase, and aryl esterase were estimated.Results: The findings revealed a significant increase in the levels of LCN2 in Arthritis compared to the control group and there was a significant decrease in the concentration of vitamin C, glutathione, vitamin E and the activity of the arylesterase in serum of patients with arthritis compared with the control group. Also, a significant increase in the activity of peroxidase, concentration of peroxynitrite and malondialdehyde for patients than a control group.Conclusion: These findings imply that LCN2 may play a substantial role in iron-related oxidative stress damage in arthritis. Thus a therapeutic candidate target for treatment.
Příspěvek je určen lékařům pro rychlou orientaci v legislativě týkající se předpisu lázeňské léčebně rehabilitační péče na úhradu z veřejného zdravotního pojištění a upozorňuje na nejčastější nedostatky při indikaci lázeňské péče.
The contribution is intended for doctors to gain a quick orientation in the legislation regarding the prescription of spa medical rehabilitation care for payment from public health insurance and draws attention to the most common shortcomings in the indication of spa care.
Herein, we describe and investigate biological activity of three octahedral ruthenium(II) complexes of the type [Ru(C∧N)(phen)2]+, RuL1-RuL3, containing a π-expansive cyclometalating substituted benzo[g]quinoxaline ligand (C∧N ligand) (phen = 1,10-phenanthroline). Compounds RuL1-RuL3 in cervical, melanoma, and colon human cancer cells exhibit high phototoxicity after irradiation with light (particularly blue), with the phototoxicity index reaching 100 for the complex RuL2 in most sensitive HCT116 cells. RuL2 accumulates in the cellular membranes. If irradiated, it induces lipid peroxidation, likely connected with photoinduced ROS generation. Oxidative damage to the fatty acids leads to the attenuation of the membranes, the activation of caspase 3, and the triggering of the apoptotic pathway, thus implementing membrane-localized photodynamic therapy. RuL2 is the first photoactive ruthenium-based complex capable of killing the hardly treatable colon cancer stem cells, a highly resilient subpopulation within a heterogeneous tumor mass, responsible for tumor recurrence and the metastatic progression of cancer.
- MeSH
- apoptóza účinky léků MeSH
- buněčná membrána účinky léků metabolismus MeSH
- chinoxaliny * chemie farmakologie chemická syntéza MeSH
- fotochemoterapie * MeSH
- fotosenzibilizující látky * farmakologie chemie chemická syntéza terapeutické užití MeSH
- komplexní sloučeniny * farmakologie chemie chemická syntéza terapeutické užití MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádorové kmenové buňky * účinky léků patologie MeSH
- nádory tračníku * farmakoterapie patologie MeSH
- protinádorové látky * farmakologie chemie chemická syntéza terapeutické užití MeSH
- reaktivní formy kyslíku metabolismus MeSH
- ruthenium * chemie farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
TP73 is a member of the TP53 gene family and produces N- and C-terminal protein isoforms through alternative promoters, alternative translation initiation and alternative splicing. Most notably, p73 protein isoforms may either contain a p53-like transactivation domain (TAp73 isoforms) or lack this domain (ΔTAp73 isoforms) and these variants have opposing or independent functions. To date, there is a lack of well-characterised isoform-specific p73 antibodies. Here, we produced polyclonal and monoclonal antibodies to N-terminal p73 variants and the C-terminal p73α isoform, the most common variant in human tissues. These reagents show that TAp73 is a marker of multiciliated epithelial cells, while ΔTAp73 is a marker of non-proliferative basal/reserve cells in squamous epithelium. We were unable to detect ΔNp73 variant proteins, in keeping with recent data that this is a minor form in human tissues. Most cervical squamous cell carcinomas (79%) express p73α, and the distribution of staining in basal cells correlated with lower tumour grade. TAp73 was found in 17% of these tumours, with a random distribution and no association with clinicopathological features. These data indicate roles for ΔTAp73 in maintaining a non-proliferative state of undifferentiated squamous epithelial cells and for TAp73 in the production of differentiated multiciliated cells.
- MeSH
- epitelové buňky metabolismus MeSH
- lidé MeSH
- monoklonální protilátky MeSH
- nádorové buněčné linie MeSH
- nádory děložního čípku metabolismus patologie genetika MeSH
- nádory metabolismus patologie genetika MeSH
- protein - isoformy * metabolismus genetika MeSH
- protein p73 * metabolismus genetika MeSH
- spinocelulární karcinom metabolismus patologie genetika MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The RNA content is crucial for the formation of nuclear compartments, such as nuclear speckles and nucleoli. Phosphatidylinositol 4,5-bisphosphate (PIP2) is found in nuclear speckles, nucleoli, and nuclear lipid islets and is involved in RNA polymerase I/II transcription. Intriguingly, the nuclear localization of PIP2 was also shown to be RNA-dependent. We therefore investigated whether PIP2 and RNA cooperate in the establishment of nuclear architecture. In this study, we unveiled the RNA-dependent PIP2-associated (RDPA) nuclear proteome in human cells by mass spectrometry. We found that intrinsically disordered regions (IDRs) with polybasic PIP2-binding K/R motifs are prevalent features of RDPA proteins. Moreover, these IDRs of RDPA proteins exhibit enrichment for phosphorylation, acetylation, and ubiquitination sites. Our results show for the first time that the RDPA protein Bromodomain-containing protein 4 (BRD4) associates with PIP2 in the RNA-dependent manner via electrostatic interactions, and that altered PIP2 levels affect the number of nuclear foci of BRD4 protein. Thus, we propose that PIP2 spatiotemporally orchestrates nuclear processes through association with RNA and RDPA proteins and affects their ability to form foci presumably via phase separation. This suggests the pivotal role of PIP2 in the establishment of a functional nuclear architecture competent for gene expression.
- MeSH
- buněčné jádro * metabolismus genetika MeSH
- fosfatidylinositol-4,5-difosfát * metabolismus MeSH
- fosforylace MeSH
- jaderné proteiny * metabolismus genetika MeSH
- lidé MeSH
- proteiny buněčného cyklu metabolismus genetika MeSH
- proteiny obsahující bromodoménu MeSH
- RNA metabolismus genetika MeSH
- transkripční faktory * metabolismus genetika MeSH
- vazba proteinů MeSH
- vnitřně neuspořádané proteiny * metabolismus genetika chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
