• Publikační typ
• abstrakt z konference MeSH

Projekt je zaměřen na vývoj a validaci UHPLC metody s využitím nejnovějšího systému UFLC-Nexera pro stanovení vitaminu D (D2 a D3) a jeho metabolitů (25-OH D2, 25-OH D3) v lidském séru s využitím moderních technologií jak v kapalinové chromatografii, tak v úpravě biologického materiálu a přípravě vzorku k analýze. Vývoj metody je iniciován požadavky klinického výzkumu Fakultní nemocnice Hradec Králové (FNHK) . Důraz je kladen nejen na vlastní výzkum studie metabolismu vitaminu D, ale i na rychlost a jednoduchost dané metody, což by umožnilo její zavedení do rutinního vyšetření např. v biochemických laboratořích. Výsledkem této studie by proto měl být přehled o hladinách vitaminu D a jeho metabolitů u vybrané skupiny pacientů. Po vyhodnocení projektu by propojení výzkumných aktivit z oblasti analytické chemie a klinického monitorování mělo vést ke zlepšení diagnostických možností a zároveň zkvalitnění léčebných postupů u závažných civilizačních chorob a zlepšení péče o jednotlivé pacienty.; The project focuses on the development and validation of UHPLC methods for determination of vitamin D and its metabolites (25-OH D2, 25-OH D3) in human serum using modern technology. Method development is initiated by clinical research requirements of the Teaching Hospital in Hradec Kralove. Emphasis is placed not only on own research studies of vitamin D metabolism, but also on the speed and simplicity of the method, which would allow its introduction into routine examinations such as biochemical laboratories. The result of this study should be therefore an overview of the levels of vitamin D and its metabolites in selected groups of patients. After evaluation of the project should link of research activities in the field of analytical chemistry and clinical monitoring lead to better diagnostic capabilities and also improve treatments for serious civilization diseases and improve the care of individual patients.

• MeSH
• biochemická analýza krve MeSH
• diagnostické techniky a postupy MeSH
• hmotnostní spektrometrie MeSH
• hypercholesterolemie diagnóza   MeSH
• klinické laboratorní techniky MeSH
• LDL-cholesterol MeSH
• separace krevních složek MeSH
• sérum MeSH
• vitamin D analýza   metabolismus   MeSH
• vysokoúčinná kapalinová chromatografie MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• chemie, klinická chemie
• biochemie
• NLK Publikační typ
• závěrečné zprávy o řešení grantu IGA MZ ČR

Vitamin D plays an important role in calcium metabolism and affects other metabolic pathways. Despite the intense interest in vitamin D, no comprehensive overview addressing the analysis of vitamin D in milk has been published. Historically, immunoassay techniques have been mainly used for the routine quantification of vitamin D and its metabolites. However, the greater accuracy and precision of chromatography makes it one of the most important methods in the analysis of vitamin D. The determination of vitamin D and its metabolites by LC-MS is the gold standard for its assessment. LC-MS has unique advantages for vitamin D determination and quantification due to its high sensitivity and specificity. In this review, the current status of vitamin D and its metabolites analysis in milk, human and bovine, including sample pre-treatment and chromatography analysis, are critically discussed and summarised.

• MeSH
• analýza potravin metody   MeSH
• chromatografie kapalinová MeSH
• extrakce kapalina-kapalina MeSH
• extrakce na pevné fázi MeSH
• hmotnostní spektrometrie MeSH
• imunoanalýza MeSH
• lidé MeSH
• mateřské mléko chemie   MeSH
• mléčné výrobky analýza   MeSH
• mléko chemie   MeSH
• senzitivita a specificita MeSH
• skot MeSH
• vitamin D analýza   MeSH
• vitaminy analýza   MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

INTRODUCTION: In the last 10 years, many studies have been published on the role of the complement system in microcirculation disorders. However, as for the changes of complement components after rheohemapheresis, there is still a lack of detailed data in the literature. Complement changes may play an important role in pathogenesis of some microcirculation disorders, such as age-related macular degeneration and acute hearing loss. The objective of this study was to investigate the effect of rheohemapheresis on the basic complement pathways. PATIENTS AND METHODS: 32 patients were treated with rheohemapheresis, including 16 patients (10 men and 6 women) for age-related macular degeneration (AMD), mean age 69.7 ± 6.06 years (range 62-87 years) and 16 patients (11 men and 5 women) aged 56.4 ± 11.5 (range 34-73 years) for acute hearing loss. RESULTS: Rheohemapheresis led to a significant drop of all three complement-activation pathways in both groups of patients. Moreover, complement factor H was also reduced. CONCLUSION: The observed reduction in all three basic complement activation pathways after rheohemapheresis could be clinically important. The search continues both to find substances which influence complement systems and to develop more effective new drugs that require less frequent administration and that provide improved intraocular therapy for AMD patients.

• MeSH
• aktivace komplementu * MeSH
• komplement - faktor H metabolismus   MeSH
• komplement metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• makulární degenerace krev   diagnóza   imunologie   terapie   MeSH
• náhlá nedoslýchavost krev   diagnóza   imunologie   terapie   MeSH
• percepční nedoslýchavost krev   diagnóza   imunologie   terapie   MeSH
• reologie krve * MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• separace krevních složek metody   MeSH
• výsledek terapie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Microplates are routinely used in Radio- or Immuno-assays. Recently, microplates have found use not only in analytical but also in the pre-analytical phase in bioanalyses (sample storage, sample preparation). New connection of this technology to liquid chromatography could be economical, fast and simple solution for many routine laboratories handling large sequences of biological samples. This review summarises the application of microplates in bioanalytical laboratories. Different types of sorbents, materials and shapes of microplates are discussed, and the main advantages and disadvantages of microplates used in clinical research are presented.

• MeSH
• biomedicínský výzkum přístrojové vybavení   MeSH
• chromatografie kapalinová přístrojové vybavení   MeSH
• laboratoře nemocniční MeSH
• laboratorní automatizace MeSH
• lidé MeSH
• odběr biologického vzorku přístrojové vybavení   metody   MeSH
• plastické hmoty * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

• Publikační typ
• abstrakt z konference MeSH

• Publikační typ
• abstrakt z konference MeSH

INTRODUCTION: Rare cases of pregnancy in women with homozygous familial hypercholesterolemia (HFH) have been reported. HFH might pose significant risks for the mother and her fetus. Statins, the most potent agents for low-density lipoprotein (LDL) cholesterol reduction, are contraindicated; thus lipoprotein apheresis remains the only effective treatment. CASE REPORT: We report on a 34-year-old pregnant woman with HFH who was treated throughout the entire pregnancy by lipoprotein apheresis (immunoadsorption method). Increasing levels of LDL-cholesterol were stabilized at 9-10 mmol/L by lipoprotein apheresis (performed every 10 days). No complications were observed during the treatment procedures. Monitoring of the fetus revealed no impairment of the umbilical cord and blood flow in the uterine arteries, as well as no intrauterine growth retardation. The delivery was spontaneous and the child was breastfed for two months. CONCLUSION: Intensive treatment by lipoprotein apheresis is an effective and safe therapeutic strategy during pregnancy, even in severe cases of HFH, as it can stabilize progressively increasing lipoprotein levels and prevent severe complications.

• MeSH
• biologické markery krev   MeSH
• časové faktory MeSH
• fenotyp MeSH
• genetická predispozice k nemoci MeSH
• homozygot * MeSH
• hyperlipoproteinemie typ II krev   diagnóza   genetika   terapie   MeSH
• imunosorpční techniky * MeSH
• komplikace těhotenství krev   diagnóza   genetika   terapie   MeSH
• LDL-cholesterol krev   MeSH
• LDL-receptory genetika   MeSH
• lidé MeSH
• mutace * MeSH
• mutační analýza DNA MeSH
• narození živého dítěte MeSH
• separace krevních složek metody   MeSH
• stupeň závažnosti nemoci MeSH
• těhotenství MeSH
• výsledek terapie MeSH
• Check Tag
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH

• Publikační typ
• abstrakt z konference MeSH

Lipid apheresis (extracorporeal lipoprotein elimination) is administered to patients with familial hypercholesterolemia who fail to respond to standard therapy. The nature of the treatment process raises the suspicion that it decreases not only cholesterol but also antioxidants. A group of 12 patients (average age 47±17 y, 4 homozygous and 8 heterozygous individuals) with familial hypercholesterolemia treated by LDL-apheresis or rheohaemapheresis for 3-12 y was included in the study. In addition to cholesterol and triacylglycerol levels, vitamin E and vitamin A and also other markers of antioxidant activity were investigated. Nevertheless, the most important determined parameter was the vitamin E/cholesterol ratio in serum and lipoproteins. The results indicate that both extracorporeal elimination methods are effective and suitable ways to treat severe familial hypercholesterolemia, as the LDL fraction of cholesterol decreased by approximately 77% and 66% following LDL-apheresis and rheohaemapheresis, respectively. In addition, the serum vitamin E decreased by 54% and 57% and the decrease of the serum vitamin A was approximately 20%. However, the main marker of antioxidant capacity, vitamin E/cholesterol ratio, in the serum, VLDL and LDL significantly increased. The increase of vitamin E levels in the erythrocyte membranes of 2% following LDL-apheresis and a significant increase of 4% following rheohaemapheresis were confirmed. The presented results indicate that LDL-apheresis and rheohaemapheresis can be considered to be safe procedures according to the antioxidant capacity of the serum, VLDL and LDL lipoprotein fractions and the erythrocyte membrane.

• MeSH
• antioxidancia metabolismus   MeSH
• cholesterol krev   MeSH
• dospělí MeSH
• erytrocyty metabolismus   MeSH
• hyperlipoproteinemie typ II terapie   MeSH
• LDL-cholesterol krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lipoproteiny krev   MeSH
• mladý dospělý MeSH
• separace krevních složek metody   MeSH
• triglyceridy krev   MeSH
• vitamin A krev   MeSH
• vitamin E krev   MeSH
• VLDL-cholesterol krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH