Common Provenance Model
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The distributed nature of modern research emphasizes the importance of collecting and sharing the history of digital and physical material, to improve the reproducibility of experiments and the quality and reusability of results. Yet, the application of the current methodologies to record provenance information is largely scattered, leading to silos of provenance information at different granularities. To tackle this fragmentation, we developed the Common Provenance Model, a set of guidelines for the generation of interoperable provenance information, and to allow the reconstruction and the navigation of a continuous provenance chain. This work presents the first version of the model, available online, based on the W3C PROV Data Model and the Provenance Composition pattern.
- MeSH
- biologické vědy * MeSH
- reprodukovatelnost výsledků MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Compared with the general population, the incidence of young-onset (YO) colorectal cancer (CRC) is increasing. However, a significant knowledge gap exists in the clinical characteristics, treatment patterns, and outcomes for these patients. MATERIALS AND METHODS: Six international tertiary cancer centers conducted a retrospective study. Patients with YO CRC (aged 18-44 years) and LO CRC (aged > 44 years) diagnosed with histologically proven colorectal adenocarcinoma from June 2003 to June 2014 were enrolled. Patients were randomly chosen from each center's database, and the patient demographics and treatment information were collected. The data were then centralized, and the final analysis was performed at a single institution. Cox proportional hazards models were used to estimate the crude and adjusted hazard ratios (HRs) with 95% confidence intervals (CIs) for progression-free survival and mortality, and YO was compared with LO. Site-specific HRs were pooled using a random-effects meta-analysis. RESULTS: Overall, 498 patients, including 224 with YO (129 men; mean age, 37 ± 5.5 years) and 274 with LO (167 men; mean age, 64.8 ± 9.5 years) CRC, were included. At the diagnosis, 137 patients (61.2%) and 122 patients (44.5%) with YO and LO CRC had metastatic disease, respectively. For both cohorts, the 3 most common presenting symptoms were pain, hematochezia, and weight loss. Surgery was performed in 141 YO (63.0%) and 219 LO (79.9%) patients. The longitudinal noncurative treatment patterns were similar, but more biologic therapy was used for these YO patients. The pooled progression-free survival analysis results for first-line noncurative treatment favored LO (HR, 1.96; 95% CI, 1.04-3.68). The mortality analysis showed no significant differences between the 2 groups (YO: HR, 1.53; 95% CI, 0.91-2.58). CONCLUSION: Despite similar treatment patterns and survival outcomes, YO disease might be clinically more aggressive.
- MeSH
- adenokarcinom epidemiologie patologie terapie MeSH
- biologická terapie metody MeSH
- dospělí MeSH
- incidence MeSH
- kolorektální nádory epidemiologie patologie terapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- metastázy nádorů MeSH
- mezinárodní spolupráce MeSH
- mladý dospělý MeSH
- přežití bez známek nemoci MeSH
- proporcionální rizikové modely MeSH
- retrospektivní studie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- věk při počátku nemoci MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
BACKGROUND: Serum uric acid (UA) elevation is common in patients with cardiovascular, renal and metabolic diseases. However, no study to date has analysed the role of UA in Fabry disease (FD). OBJECTIVES: To evaluate the association between serum UA levels and mortality and morbidity in FD. MATERIALS AND METHODS: We conducted a post-hoc analysis of a prospectively followed-up cohort of 124 patients with genetically proven FD. Serum UA levels were acquired at baseline; clinical events and mortality were assessed during regular visits every 6 to 12 months. The primary endpoint was a composite of multiple secondary outcomes: all-cause mortality, adverse cardiovascular events, progression of renal dysfunction and stroke or transient ischaemic attack (TIA). Predictive value was assessed using the Cox proportional hazards model and the Kaplan Meyer estimator. RESULTS: During follow-up of 7.4 ± 3.7 years, 64 (52%) patients reached the primary combined endpoint. Overall, UA levels were significantly associated with combined outcome (p < 0.001) and remained independently associated after correcting for age, sex and estimated glomerular filtration rate (hazard ratio [HR] per 20 μmol/l increase 1.09, 95% confidence interval [95%CI] (1.00-1.19), p = 0.04). UA was associated with overall mortality in univariate analysis (p = 0.021); however, the association did not reach statistical significance after multivariate correction (HR per 20 μmol/l increase 1.07 95%CI 0.93-1.25, p = 0.32). Higher UA levels were also associated with cardiac adverse outcomes, progression of left ventricular hypertrophy and progression of renal dysfunction (ps < 0.001). No association was observed between UA levels and stroke or TIA (p = 0.323). CONCLUSION AND IMPLICATIONS: Increased serum UA levels may represent an independent risk factor for adverse clinical outcomes in Fabry patients and are associated with all-cause mortality. UA is a widely available and cheap biomarker that may improve risk stratification of Fabry patients in clinical practice.
- MeSH
- biologické markery krev MeSH
- cévní mozková příhoda epidemiologie MeSH
- dospělí MeSH
- Fabryho nemoc krev epidemiologie mortalita MeSH
- hodnocení výsledků zdravotní péče metody statistika a číselné údaje MeSH
- Kaplanův-Meierův odhad MeSH
- kyselina močová krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- míra přežití MeSH
- mladý dospělý MeSH
- morbidita MeSH
- následné studie MeSH
- progrese nemoci MeSH
- proporcionální rizikové modely MeSH
- prospektivní studie MeSH
- tranzitorní ischemická ataka epidemiologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
We recently developed a new light source that allows for the continuous monitoring of light-induced changes using common spectrophotometric devices adapted for microplate analyses. This source was designed primarily to induce photodynamic processes in cell models. Modern light components, such as LED chips, were used to improve the irradiance homogeneity. In addition, this source forms a small hermetic chamber and thus allows for the regulation of the surrounding atmosphere, which plays a significant role in these light-dependent reactions. The efficacy of the new light source was proven via kinetic measurements of reactive oxygen species generated during the photodynamic reaction of chloroaluminium phthalocyanine disulfonate (ClAlPcS2) in three cell lines: human melanoma cells (G361), human breast adenocarcinoma cells (MCF7), and human fibroblasts (BJ).
Understanding mitochondrial role in normal physiology and pathological conditions has proven to be of high importance as mitochondrial dysfunction is connected with a number of disorders as well as some of the most common diseases (e.g. diabetes or Parkinson's disease). Modeling mitochondrial dysfunction has been difficult mainly due to unique features of mitochondrial genetics. Here we discuss some of the most important mouse models generated so far and lessons learned from them.
- MeSH
- diabetes mellitus etiologie genetika imunologie MeSH
- financování organizované MeSH
- genetické techniky využití MeSH
- metaanalýza jako téma MeSH
- metabolický syndrom etiologie genetika imunologie MeSH
- mitochondriální DNA fyziologie genetika imunologie MeSH
- mitochondriální encefalomyopatie etiologie genetika imunologie MeSH
- mitochondriální nemoci etiologie genetika imunologie MeSH
- modely u zvířat MeSH
- myši MeSH
- neurodegenerativní nemoci etiologie genetika imunologie MeSH
- Parkinsonova nemoc etiologie genetika imunologie MeSH
- stárnutí genetika imunologie MeSH
- Check Tag
- myši MeSH
- Publikační typ
- přehledy MeSH
Objectives: Presentation of an overview of the reasons why data integration initiatives should be seen as enablers for effective decision support in data-intensive healthcare settings. Methods: Typical challenges rising from the information requirements of clinical decision support systems are highlighted. We then propose a methodological solution where several heterogeneous data sources are integrated by the means of a common data model on top of which the DSS is built. Results: We report on two successful case studies based on the DSSs developed in the context of the MobiGuide and Mosaic projects, funded by the European Union in the Seventh Framework Program. The MobiGuide patient guidance system has been successfully validated during a recent pilot study involving 30 patients (10 with atrial fibrillation and 20 with gestational diabetes), while Mosaic is currently undergoing a validation phase involving 1000 type 2 Diabetes patients. Conclusions: In the era of big data, effective data integration strategies are an essential need for medical informatics solutions and even more for those intended to support decision processes. Building generic DSSs based on a stable (but easily extensible) data model, specifically designed to meet the information requirements of DSSs and analytics, has proven to be a successful solution in the two presented use cases.
- Klíčová slova
- projekt MOSAIC, projekt MobiGuide,
- MeSH
- diabetes mellitus 2. typu diagnóza terapie MeSH
- fibrilace síní diagnóza terapie MeSH
- Health Level Seven MeSH
- informace pro uživatele zdravotní péče * MeSH
- lidé MeSH
- rozhodování pomocí počítače MeSH
- systémová integrace MeSH
- systémy pro podporu klinického rozhodování * MeSH
- teoretické modely MeSH
- zapojení pacienta MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Peak shapes in electrophoresis are often distorted from the ideal Gaussian shape due to disturbing phenomena, of which the most important is electromigration dispersion. For fully dissociated analytes, there is a tight analogy between nonlinear models describing a separation process in chromatography and electrophoresis. When the velocity of the separated analyte depends on the concentration of the co-analyte, the consequence is a mutual influence of the analytes couples, which distorts both analyte zones. In this paper, we introduce a nonlinear model of electromigration for the analysis of two co-migrating fully dissociated analytes. In the initial stages of separation, they influence each other, which causes much more complicated peak shapes. The analysis has revealed that the two most important phenomena-the displacement and the tag-along effects-are common both for nonlinear chromatography and electrophoresis, though their description is partly based on rather different phenomena. The comparison between the nonlinear model of electromigration we describe and the numerical computer solution of the original continuity equations has proven an almost perfect agreement. The predicted features in peak shapes in initial stages of separation have been fully confirmed by the experiments.
Gastric cancer is a common oncological disease. Although enormous efforts have been expended, possible therapeutic modalities are still limited. For this reason, new therapeutic approaches and agents are highly requested and intensively developed. One strategy is the application of natural agents, such as curcumin, with proven anticancer effects and low toxicity for patients. Therefore, this review discusses the potential application of curcumin in the therapy of gastric cancer and its potential incorporation in therapeutic regimens. Because one of the largest impediments for widespread curcumin application is its limited bioavailability (caused mainly by its very low water solubility), studied strategies (drug delivery systems and curcumin derivatization) aimed to solve this obstacle are discussed in more detail.
- MeSH
- biologické modely MeSH
- kurkumin chemie terapeutické užití MeSH
- lékové transportní systémy MeSH
- lidé MeSH
- nádory žaludku farmakoterapie MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Carp from breeding strains with different genetic background present diverse levels of resistance to viral pathogens. Carp strains of Asian origin, currently being treated as Cyprinus rubrofuscus L., especially Amur wild carp (AS), were proven to be more resistant to koi herpesvirus disease (KHVD; caused by cyprinid herpesvirus 3, CyHV-3) than strains originating from Europe and belonging to Cyprinus carpio L., like the Prerov scale carp (PS) or koi carp from a breed in the Czech Republic. We hypothesised that it can be associated with a higher magnitude of type I interferon (IFN) response as a first line of innate defence mechanisms against viral infections. To evaluate this hypothesis, four strains of common carp (AS, Rop, PS and koi) were challenged using two viral infection models: Rhabdovirus SVCV (spring viremia of carp virus) and alloherpesvirus CyHV-3. The infection with SVCV induced a low mortality rates and the most resistant was the Rop strain (no mortalities), whereas the PS strain was the most susceptible (survival rate of 78%). During CyHV-3 infection, Rop and AS strains performed better (survival rates of 78% and 53%, respectively) than PS and koi strains (survival rates of 35% and 10%, respectively). The evaluation of virus loads and virus replication showed significant differences between the carp strains, which correlated with the mortality rate. The evaluation of type I IFN responses showed that there were fundamental differences between the virus infection models. While responses to the SVCV were high, the CyHV-3 generally induced low responses. Furthermore, the results demonstrated that the magnitude of type I IFN responses did not correlate with a higher resistance in infected carp. In the case of a CyHV-3 infection, reduced type I IFN responses could be related to the potential ability of the virus to interfere with cellular sensing of foreign nucleic acids. Taken together, the results broaden our understanding of how common carp from different genetic strains interact with various viral pathogens.
- MeSH
- Herpesviridae fyziologie MeSH
- herpetické infekce imunologie veterinární MeSH
- infekce viry z čeledi Rhabdoviridae imunologie veterinární MeSH
- interferon typ I genetika imunologie MeSH
- kapři genetika imunologie MeSH
- nemoci ryb imunologie MeSH
- odolnost vůči nemocem genetika MeSH
- Rhabdoviridae fyziologie MeSH
- rybí proteiny genetika imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Mesial temporal lobe epilepsy (MTLE) with hippocampal sclerosis (HS) constitutes a distinct clinical syndrome with variable pathogenesis. Extrahippocampal regions may be affected in MTLE/HS, association with cortical dysplasia is common and temporal polar cortex is frequently involved in seizure onset. Patients with dual pathology may have favourable outcome from the surgery provided that both pathologies are removed. The aim of the study was to review clinical variables of MTLE/HS patients in order to distinguish preoperatively patients with associated microscopic cortical dysplasia in the temporal pole. METHODS: A series of 38 patients with the clinical diagnosis of MTLE and histopathologically proven HS were analysed. Patients were divided into two groups on the basis of histopathological finding in the temporal polar cortex: HS associated with malformation of cortical development (group HS+, n = 19) and a group with isolated HS (group HS, n = 19). Demographic, clinical, electrographic and seizure semiology variables were obtained and their prevalence compared between both groups. RESULTS: At least one insult was identified in early childhood history of 18 patients in the HS group in comparison to 10 patients in the HS+ group (p < 0.01). Complicated febrile seizures were found in both groups with similar prevalence, the history of early childhood CNS infection prevailed in the HS group (p < 0.01). Absence of aura was reported in HS group only. Patients in the the HS+ group had earlier surgery (p < 0.05) but the seizure outcome was comparable between groups. CONCLUSIONS: Microscopic dual pathology is common in MTLE/HS patients. This group of patients is difficult to distinguish preoperatively on the basis of noninvasive electrographic features or ictal clinical semiology. Detailed information regarding the possible precipitating insult in the history may be of critical importance.
- MeSH
- demografie MeSH
- dítě MeSH
- dospělí MeSH
- epilepsie temporálního laloku chirurgie komplikace patologie MeSH
- financování organizované MeSH
- hipokampus chirurgie patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- malformace mozkové kůry chirurgie komplikace patologie MeSH
- mladiství MeSH
- skleróza chirurgie komplikace patologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
