Magnetic resonance imaging (MRI) relies on appropriate contrast agents, especially for visualizing transplanted cells within host tissue. In recent years, compounds containing fluorine-19 have gained significant attention as MRI probe, particularly in dual 1H/19F-MR imaging. However, various factors affecting probe sensitivity, such as fluorine content and the equivalency of fluorine atoms, must be considered. In this study, we synthesized fluorinated micelles with adjustable surface positive charge density and investigated their physicochemical properties and MRI efficacy in phantoms and labeled cells. While the micelles exhibited clear signals in 19F-MR spectra and imaging, the concentrations required for MRI visualization of labeled cells were relatively high, adversely affecting cell viability. Despite their favourable physicochemical properties, achieving higher labeling rates without compromising cell viability during labeling remains a challenge for potential in vivo applications.

• MeSH
• barvení a značení metody   MeSH
• fantomy radiodiagnostické MeSH
• fluor chemie   MeSH
• halogenace MeSH
• kationty * chemie   MeSH
• kontrastní látky chemie   MeSH
• lidé MeSH
• magnetická rezonanční tomografie metody   MeSH
• micely * MeSH
• myši MeSH
• viabilita buněk * účinky léků   MeSH
• zobrazování fluorovou magnetickou rezonancí metody   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Theranostics is a novel paradigm integrating therapy and diagnostics, thereby providing new prospects for overcoming the limitations of traditional treatments. In this context, perfluorocarbons (PFCs) are the most widely used tracers in preclinical fluorine-19 magnetic resonance (19F MR), primarily for their high fluorine content. However, PFCs are extremely hydrophobic, and their solutions often display reduced biocompatibility, relative instability, and subpar 19F MR relaxation times. This study aims to explore the potential of micellar 19F MR imaging (MRI) tracers, synthesized by polymerization-induced self-assembly (PISA), as alternative theranostic agents for simultaneous imaging and release of the non-steroidal antileprotic drug clofazimine. In vitro, under physiological conditions, these micelles demonstrate sustained drug release. In vivo, throughout the drug release process, they provide a highly specific and sensitive 19F MRI signal. Even after extended exposure, these fluoropolymer tracers show biocompatibility, as confirmed by the histological analysis. Moreover, the characteristics of these polymers can be broadly adjusted by design to meet the wide range of criteria for preclinical and clinical settings. Therefore, micellar 19F MRI tracers display physicochemical properties suitable for in vivo imaging, such as relaxation times and non-toxicity, and high performance as drug carriers, highlighting their potential as both diagnostic and therapeutic tools.

• MeSH
• biokompatibilní materiály chemie   MeSH
• fluor chemie   MeSH
• fluorokarbony chemie   MeSH
• halogenace MeSH
• lidé MeSH
• magnetická rezonanční tomografie metody   MeSH
• micely MeSH
• myši MeSH
• nanočástice * chemie   terapeutické užití   MeSH
• teranostická nanomedicína * MeSH
• zobrazování fluorovou magnetickou rezonancí * metody   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

We developed a multifunctional polymer coating for nanoparticles (NPs) that enables simultaneous detection by 19F MRI and shielding from blood plasma fouling. The coating is based on a water-soluble fluorinated poly(N-(2-fluoroethyl)acrylamide) (PFEAM) that shows high 19F MRI sensitivity, cytocompatibility and excellent antifouling properties, significantly outperforming polyethylene glycol. A proof-of-concept experiment was performed by synthesizing polymer-coated gold NPs that were successfully visualized by 19F MRI at magnetic fields close to the fields used in clinical practice. This universal approach can be used for coating and tracing of various NPs upon suitable polymer chain-end modification.

• MeSH
• akrylové pryskyřice chemie   farmakologie   MeSH
• biokompatibilní potahované materiály chemie   farmakologie   MeSH
• bioznečištění prevence a kontrola   MeSH
• fluor MeSH
• halogenace MeSH
• lidé MeSH
• magnetická rezonanční tomografie * MeSH
• nanostruktury chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

This article describes the function and possible utilization of the fluorine line in the title Institute. It also focuses on the improved procedure for the conversion of the commercial salt 1H-CB11H11 – to 1H-CB11F11 – on a gram scale.

• MeSH
• anorganická chemie metody   trendy   MeSH
• borany chemie   MeSH
• chemické jevy * MeSH
• fluor * chemie   MeSH
• halogenace * MeSH
• kyselina fluorovodíková chemie   MeSH
• lidé MeSH
• molekulární zobrazování * MeSH
• nanostruktury MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH