UNLABELLED: Wolbachia pipientis is an obligate intracellular bacterium, associated with several arthropods and filarial nematodes. Wolbachia establishes a variety of symbiotic relationships with its hosts, with consequent genomic rearrangements, variation in gene content, and loss of regulatory regions. Despite this, experimental studies show that Wolbachia gene expression is coordinated with host developmental stages, but the mechanism is still unknown. In this work, we analyzed published RNA-seq data of four Wolbachia strains, finding a correlation between gene nucleotide composition and gene expression. The strength and direction of this phenomenon changed with the expression of the S-adenosyl-methionine-dependent methyltransferase midA. Specifically, when midA is overexpressed, there is a negative relationship between gene adenine content and gene expression, while downregulation of midA reverses this trend. MidA is known to methylate protein arginine, with potential effect on protein affinity for substrates, including nucleic acids. To expand our understanding of this poorly characterized enzyme, we investigated its ability to methylate DNA expressing it in Escherichia coli. The experiment revealed that the Wolbachia MidA can methylate both adenine and cytosine. Lastly, we found upstream the midA gene, a conserved binding site for the Ccka/CtrA signaling transduction system, and we hypothesize that this mechanism could be involved in the communication between the host and the bacterium. Overall, these findings suggest a cascade mechanism in which the host activates the bacterium Ccka/CtrA signaling system, thus inducing the expression of the midA gene, with subsequent effect on the expression of several Wolbachia genes on the basis of their nucleotide composition. IMPORTANCE: Wolbachia pipientis is one of the most common intracellular bacteria in insects, and it is currently utilized as a tool for the control of vector-borne diseases. As for many other endosymbiont bacteria, Wolbachia experienced important genome rearrangements, gene content changes, and the loss of several regulatory sequences, affecting the integrity of operons and promoters. Nevertheless, experimental studies have shown that Wolbachia gene expression is coordinated with the host physiology (e.g., developmental stages), although the underlying mechanism remains unclear. In this work, based on in silico analyses and an experimental study on wOo methyltransferase, we propose that bacterial DNA methylation could be a key mechanism regulating Wolbachia gene expression. Additionally, we found evidence suggesting that the DNA methylation process in Wolbachia can be activated by the host.

• MeSH
• bakteriální proteiny * genetika   metabolismus   MeSH
• methyltransferasy * genetika   metabolismus   MeSH
• metylace DNA MeSH
• nukleotidy * genetika   MeSH
• regulace genové exprese u bakterií * MeSH
• Wolbachia * genetika   enzymologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Six Dipetalonema species have been reported from Neotropical monkeys, Dipetalonema gracile, Dipetalonema graciliformis and Dipetalonema caudispina being the dominant species found in French Guiana primates. Adult filarioids isolated from the abdominal cavity of tamarins (Saguinus midas) in French Guiana were morphologically and molecularly identified as D. graciliformis. Phylogenetic analysis based on DNA and amino acid sequences of the cox1 gene as well as the concatenated sequences of the cox1 and the 18S genes indicated that D. graciliformis belongs to the clade 4 (ONC4) of Onchocercidae. Blast analysis of the 18S rDNA revealed that D. graciliformis in the studied tamarins is conspecific with the filarioid circulating in howler monkeys (Alouatta macconnelli) in French Guiana, previously referred to as unidentified Onchocercidae species.

• MeSH
• Dipetalonema anatomie a histologie   klasifikace   izolace a purifikace   MeSH
• infekce hlísticemi rodu Dipetalonema epidemiologie   parazitologie   veterinární   MeSH
• nemoci opic epidemiologie   parazitologie   MeSH
• Saguinus parazitologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Francouzská Guyana MeSH

OBJECTIVE: To develop and validate new Juvenile Arthritis Disease Activity Score 10 (JADAS10) and clinical JADAS10 (cJADAS10) cutoffs to separate the states of inactive disease (ID), minimal disease activity (MiDA), moderate disease activity (MoDA), and high disease activity (HDA) in children with oligoarthritis and with rheumatoid factor-negative polyarthritis, based on subjective disease assessment by the treating pediatric rheumatologist. METHODS: The cutoffs definition cohort was composed of 1,936 patients included in the multinational Epidemiology, Treatment and Outcome of Childhood Arthritis (EPOCA) study. Using the subjective physician rating as an external criterion, 4 methods were applied to identify the cutoffs: mapping, Youden index, 90% specificity, and maximum agreement. The validation cohort included 4,014 EPOCA patients, patients from 2 randomized trials, and 88 patients from the PharmaChild registry. Cutoff validation was conducted by assessing discriminative and predictive ability. RESULTS: The JADAS10 cutoffs were 1.4, 4, and 13, respectively, for oligoarthritis and 2.7, 6, and 17, respectively, for polyarthritis. The cJADAS10 cutoffs were 1.1, 4, and 12, respectively, for oligoarthritis and 2.5, 5, and 16, respectively, for polyarthritis. The cutoffs discriminated strongly among different levels of pain and morning stiffness, between patients who were and those who were not prescribed a new medication, and between different levels of improvement in clinical trials. Achievement of ID and MiDA according to the new JADAS cutoffs at least twice in the first year of disease predicted better outcome at 2 years. CONCLUSION: The 2021 JADAS and cJADAS cutoffs revealed good metrologic properties in both definition and validation samples, and are therefore suitable for use in clinical trials and routine practice.

• MeSH
• dítě MeSH
• juvenilní artritida krev   diagnóza   MeSH
• lidé MeSH
• registrace MeSH
• revmatoidní faktor krev   MeSH
• revmatologie * MeSH
• stupeň závažnosti nemoci MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• validační studie MeSH

• MeSH
• internacionalita MeSH
• migrace lidstva MeSH
• populační dynamika MeSH
• práce MeSH
• socioekonomické faktory MeSH
• zaměstnanost MeSH

• Konspekt
• Sociologie
• NLK Obory
• sociologie
• demografie
• NLK Publikační typ
• CD-ROM

PedMIDAS (the Pediatric Migraine Disability Assessment) je dotazník pro určení míry disability specificky navržený pro použití u dětí školního věku a adolescentů trpících migrénou, je obdobou dotazníku MIDAS (the Migraine Disability Assessment) používaného u dospělých pacientů. Zohledňuje specifické aspekty životního stylu dětí i jejich subjektivní vnímání bolesti a jejího vlivu na každodenní život. Skládá se, na rozdíl od dotazníku pro dospělé, z šesti otázek a i jeho bodové hodnocení je odlišné. PedMIDAS poskytuje možnost k jednoduchému a validnímu zhodnocení vlivu migrény na kvalitu života dětských pacientů a dále možnost správného zvolení léčebné strategie, monitoraci odpovědi na léčbu a také vývoje onemocnění v čase. Na rozdíl od široce rozšířeného dotazníku MIDAS u dospělých migreniků, není u nás dětská verze používána.

The PedMIDAS (the Paediatric Migraine Disability Assessment) is a questionnaire which was specifically developed to assess the level of disability due to migraines in children and adolescents. It is analogous to the MIDAS (the Migraine Disability Assessment) tool used in adults. It takes into account the specific aspects of children`s lifestyles and their subjective interpretation of pain and its influence on their life. The PedMIDAS consists, in contrast to questionnaires for adults, of six individual questions and the evaluation thereof also differs from that of MIDAS. The PedMIDAS questionnaire provides the possibility for a simple and valid assessment of the impact of migraine on the quality of life of paediatric patients. There is also an option to select the correct medication, and to monitor the response to treatment and the development of the disorder over time. Unlike the MIDAS questionnaire used in adult migraine patients, the paediatric version is not used in the Czech Republic.

• MeSH
• dítě MeSH
• kvalita života psychologie   MeSH
• lidé MeSH
• migréna diagnóza   psychologie   MeSH
• mladiství MeSH
• poradenství pro děti metody   MeSH
• předškolní dítě MeSH
• průzkumy a dotazníky využití   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• Publikační typ
• přehledy MeSH

OBJECTIVE: Patients with chronic idiopathic intracranial hypertension (IIH) commonly experience a high level of disability and low satisfaction with medical treatment. We aim to evaluate long-term functional improvement and patient satisfaction in IIH patients with similar symptoms by comparing venous sinus stenting (VSS) to standard medical therapy. METHODS: We conducted a cross-sectional questionnaire study of 111 IIH patients, comparing 37 adult female patients who underwent venous sinus stenting with 74 patients treated medically. Propensity score matching was used to balance age and presence of papilledema at presentation between groups. Headache-related disability was evaluated using the Migraine Disability Assessment Scale (MIDAS), while general function and treatment satisfaction were assessed using custom questionnaires. Electronic medical records and the results of imaging upon diagnosis were reviewed retrospectively. RESULTS: The stented group reported significantly better outcomes in physical well-being (median 4.0 vs. 1.0, p < 0.001), task completion (4.0 vs. 1.0, p < 0.001), work/school persistence (5.0 vs. 1.0, p < 0.001), and mental well-being (4.0 vs. 1.0, p < 0.001). Additionally, the stented group had a lower proportion of patients with severe MIDAS (MIDAS > 4, 24.3% vs. 47.9%, p = 0.017). Logistic regression suggested venous stenting as a protective factor against severe MIDAS scores (OR = 0.174, p = 0.004). CONCLUSION: Cerebral venous stenting in patients with IIH is associated with lower disability and higher patient satisfaction from medical treatment compared to those treated with medications only. These findings suggest that venous sinus stenting may be a valuable treatment option for selected IIH patients. However, larger prospective studies are needed to further validate our results.

• Publikační typ
• časopisecké články MeSH

V rámci biologického monitoringu byly v roce 2005 sledovány hladiny kadmia, olova, rtuti, mìdi, selenu a zinku v krvi a moèi dospìlé populace. Koncentrace kadmia v krvi kuøákù (medián) byla vyšší než u nekuøákù (1,30 vs. 0,50 μg/l). U žen byly nalezeny vyšší koncentrace kadmia v moèi ve srovnání s muži, u obou pohlaví byl pozorován vzestup hladin kadmia v moèi s vìkem. Koncentrace olova v krvi byly vyšší u mužù (35,4 μg/l) než u žen (26,6 μg/l) a stoupaly s vìkem. Koncentrace rtuti v krvi u mužù a žen byly 0,91 a 1,16 μg/l. Hladina selenu v krvi dospìlých byla 111 μg/l, hodnoty mìdi a zinku v krvi byly v normì a stabilizované. Muži mìli významnì nižší koncentrace mìdi v krvi ve srovnání se ženami neužívajícími a užívajícími orální antikoncepci (870 vs. 950 vs. 1305 μg/l).

In 2005 the concentrations of trace elements (cadmium, lead, mercury, copper, selenium, zinc) in blood and urine of adults were measured in the framework of the Biological Monitoring Project in the Czech Republic. Blood cadmium levels in adults were significantly influenced by smoking (medians 1.30 and 0.50 μg/l in smokers and non-smokers, respectively). The levels of cadmium in urine were higher in women than in men. Higher blood lead levels were observed in men (35,4 μg/l) in comparison to women (26,6 μg/l), plumbemia correlated with age. Blood mercury levels were 0.91 and 1.16 μg/l in men and women, respectively. Blood selenium levels were 111 μg/l. Blood copper and zinc levels are stabilized. Significantly lower blood copper levels were found in men in comparison with female non-users and users of oral contraception (870, 950 and 1305 μg/l, respectively).

• MeSH
• kadmium krev   moč   MeSH
• kontraceptiva orální MeSH
• lidé MeSH
• měď krev   moč   MeSH
• olovo krev   moč   MeSH
• poruchy vyvolané užíváním tabáku MeSH
• referenční hodnoty MeSH
• rozložení podle pohlaví MeSH
• rtuť krev   moč   MeSH
• selen krev   moč   MeSH
• vystavení vlivu životního prostředí MeSH
• xenobiotika krev   moč   MeSH
• zinek krev   moč   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

Mid-dermální elastolýza (MDE) je vzácné kožní onemocnění nejasné etiologie, postihující především mladší ženy. V patogenezi se uvažuje o roli ultrafialového záření, histopatologicky charakterizované ztrátou elastických vláken ve střední úrovni dermis a klinicky se projevující okrsky jemně svraštělého povrchu kůže. Autoři popisují případ 34leté nemocné, která v minulosti navštěvovala solária, s dvouletou anamnézou kožních projevů histologicky potvrzené MDE postihující trup, zejména horní přední polovinu hrudníku. Po dvou letech sledování a aplikaci volně dostupných preparátů s obsahem sóji byl nález stacionární, bez progrese. Klíčová slova: mid-dermální elastolýza – svraštělá kůže

Mid-dermal elastolysis (MDE) is a rare skin disease of unknown etiology affecting epecially young women. The role of ultraviolet light is speculated in the pathogenesis of the disease which is histolopathologically characterised by the loss of elastic fibres in the middle dermis and clinically by the wrinked skin surface. Authors report a case of 34-year old women with history of tanning bed exposure presenting with skin lesions on the trunk especially the upper anterior part of the chest histopathologically verified as MDE. After 2 years of follow-up and local therapy with over the counter soya preparations the skin lesions remained stable. Key words: mid-dermal elastolysis – wrinkled skin

• MeSH
• dospělí MeSH
• elastická tkáň * patologie   MeSH
• kožní nemoci diagnóza   etiologie   terapie   MeSH
• lidé MeSH
• slunění MeSH
• ultrafialové záření * škodlivé účinky   MeSH
• výsledek terapie MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

Background: The Medical Information Database Network (MID-NET) is a national project that promotes effective safety measures for the active surveillance of drug safety assessments through pharmacoepidemiological methods, using real-world data in Japan. The MID-NET contains the data of approximately 5.05 million patients (as of December 2019) across 10 medical institutions, including 23 hospitals. One of the most important conditions for conducting pharmacoepidemiological research using multiple medical databases is to systematically verify of data standardization. Objectives: To evaluate the effect of improving the accuracy of standard data quality control by the development of a validation model for standard code mapping in multiple medical information databases. Methods: We established the standard code mapping validation center at one of the cooperating medical institutions of the MID-NET that could collect and manage information about the standard code interoperability. Additionally, we used the mapping table for the four standard codes, including the Japan Laboratory Test Standard Code, 10th Revision (JLAC10) code were collected from MID-NET cooperating institutions, and the accuracy of the mapping table was evaluated. Results: The observed four standard codes mapping ratio between institutions varied from >2,000 to <100. Moreover, the accuracies of standard codes were not standardized. We used a centralized standard code mapping validation model to provide feedback for standardizing JLAC-10 for each institution and meaningful differences between institutions were improved. Conclusions: The developed model visualized information differences and improved the data quality between multiple medical institutions.

• MeSH
• farmakoepidemiologie MeSH
• informační systémy * MeSH
• lidé MeSH
• řízení kvality MeSH
• správnost dat MeSH
• validační studie jako téma MeSH
• výměna zdravotnických informací MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH

High golf performance depends on ability to repeat golf swing execution with all golf clubs and reach repetitive contact between the golf club and the ball. The aim of the study was to determine the variability of golf swing execution in lower and upper body parameters for two golf clubs. Eleven elite female golfers of junior age volunteered in the study and performed 10 trials with mid-iron and with driver at laboratory conditions. 3D motion capture system was used to measure the lower and upper body movement parameters and club head speed. To assess variability the coefficient of variation and biological coefficient of variation were used. Two-tailed t test was used to identify difference between mid-iron and driver variability for all kinematic variables. Club effect was found at top of the backswing in shoulders tilt (p = 0.02) and at impact in shoulders rotation (p = 0.01), shoulders tilt (p = 0.02) and club head speed (p = 0.001). We did not found any significant difference in variability of body movement kinematic parameters and temporal parameters between mid-iron and driver. We found high stability of performance in temporal parameters and for lower limbs kinematic parameters at three selected moments of the golf swing for both clubs. We suggest that stability of temporal parameters is the key to achieve the effective and repeatable golf swing in all golf clubs.

High golf performance depends on ability to repeat golf swing execution with all golf clubs and reach repetitive contact between the golf club and the ball. The aim of the study was to determine the variability of golf swing execution in lower and upper body parameters for two golf clubs. Eleven elite female golfers of junior age volunteered in the study and performed 10 trials with mid-iron and with driver at laboratory conditions. 3D motion capture system was used to measure the lower and upper body movement parameters and club head speed. To assess variability the coefficient of variation and biological coefficient of variation were used. Two-tailed t test was used to identify difference between mid-iron and driver variability for all kinematic variables. Club effect was found at top of the backswing in shoulders tilt (p = 0.02) and at impact in shoulders rotation (p = 0.01), shoulders tilt (p = 0.02) and club head speed (p = 0.001). We did not found any significant difference in variability of body movement kinematic parameters and temporal parameters between mid-iron and driver. We found high stability of performance in temporal parameters and for lower limbs kinematic parameters at three selected moments of the golf swing for both clubs. We suggest that stability of temporal parameters is the key to achieve the effective and repeatable golf swing in all golf clubs.

• MeSH
• biomechanika * MeSH
• golf MeSH
• kineziologie aplikovaná metody   MeSH
• lidé MeSH
• pánev MeSH
• rameno MeSH
• výzkum MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH