Liposarkomy patří mezi zhoubné nádory měkkých tkání. Myxoidní liposarkom je druhým nejčastějším podtypem takového nádoru u dospělých a tvoří asi 20 % maligních nádorů měkkých tkání [1,2] s vrcholem výskytu v rozmezí okolo 40–60 let. Je charakteristický pomalým růstem, s relativně dlouhým, klinicky němým průběhem (Matsumoto a spol. [2007] [3], Cho a spol. [2010] [4], Fairman a spol. [2005] [5]). Typickou lokalizací myxoidního liposarkomu je tuková tkáň končetin, zejména dolních v oblasti stehen. Nádor má tendenci metastazovat do extrapulmonárních míst, jako jsou retroperitoneum, mediastinum a kosti. Nález metastáz v srdci je velmi vzácný [2,6]. Předmětem tohoto sdělení je případ 36letého muže s anamnézou recidivujícího myxoidního liposarkomu. Primární ložisko nádoru se nacházelo v levé popliteální oblasti. Po exstirpaci došlo k rekurenci nádoru do pravé axilární oblasti. Obě ložiska byla resekována a byla aplikována brachyterapie a zevní ozáření. V rámci stagingu bylo provedeno vyšetření pomocí PET/CT, které popsalo nově vzniklý nejasný útvar v oblasti srdečního mezikomorového septa. Nemocný byl po subjektivní stránce bez obtíží a byl odeslán k dalšímu vyšetření na naše pracoviště. Zde provedenými zobrazovacími metodami byla lokalizace nádoru potvrzena a byl chirurgicky odstraněn. Histologickým vyšetřením byla potvrzena diagnóza myxoidního liposarkomu.
Liposarcomas are malignant tumors of the soft tissue. Myxoid liposarcoma is the second most common subtype of these tumors in adults. It accounts for approximately 20% of all malignant soft tissue tumors [1,2]. Peak of its incidence occurs between 40 and 60 years of age with relatively indolent clinical course (Matsumoto et al. [2007] [3], Cho et al. [2010] [4], Fairman et al. [2005] [5]). Typical localizations of myxoid liposarcoma comprise limbs, particularly thighs with a tendency to metastasize into extrapulmonary sites such as retroperitoneum, mediastinum, bones. Cardiac metastases are extremely rare. We present a case of a 36-year-old man with a history of recurrent myxoid liposarcoma. Primary location was in the left popliteal area. After extirpation of the tumor, metastatic tumor was subsequently revealed in the right axilla. Each surgical extirpation was followed by actinotherapy and brachytherapy. Cardiac metastasis was diagnosed randomly, with PET CT during the staging process. The patient was asymptomatic and was admitted to our institution for further diagnostics and treatment. After confirmation of its location, the tumor was excised. Histological examination revealed myxoid liposarcoma.
- Keywords
- srdeční metastázy,
- MeSH
- Adult MeSH
- Echocardiography MeSH
- Humans MeSH
- Metastasectomy MeSH
- Neoplasm Metastasis * diagnosis therapy MeSH
- Ventricular Septum * pathology MeSH
- Liposarcoma, Myxoid * diagnosis etiology surgery radiotherapy secondary MeSH
- Neoplasm Staging MeSH
- Tomography, Emission-Computed MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
BACKGROUND: Breast carcinomas represent a heterogeneous group of tumors diverse in behavior, outcome, and response to therapy. Identification of proteins resembling the tumor biology can improve the diagnosis, prediction, treatment selection, and targeting of therapy. Since the beginning of the post-genomic era, the focus of molecular biology gradually moved from genomes to proteins and proteomes and to their functionality. Proteomics can potentially capture dynamic changes in protein expression integrating both genetic and epigenetic influences. METHODS: We prepared primary cultures of epithelial cells from 23 breast cancer tissue samples and performed comparative proteomic analysis. Seven patients developed distant metastases within three-year follow-up. These samples were included into a metastase-positive group, the others formed a metastase-negative group. Two-dimensional electrophoretical (2-DE) gels in pH range 4-7 were prepared. Spot densities in 2-DE protein maps were subjected to statistical analyses (R/maanova package) and data-mining analysis (GUHA). For identification of proteins in selected spots, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed. RESULTS: Three protein spots were significantly altered between the metastatic and non-metastatic groups. The correlations were proven at the 0.05 significance level. Nucleophosmin was increased in the group with metastases. The levels of 2,3-trans-enoyl-CoA isomerase and glutathione peroxidase 1 were decreased. CONCLUSION: We have performed an extensive proteomic study of mammary epithelial cells from breast cancer patients. We have found differentially expressed proteins between the samples from metastase-positive and metastase-negative patient groups.
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Adult MeSH
- Epithelial Cells metabolism MeSH
- Financing, Organized MeSH
- Middle Aged MeSH
- Humans MeSH
- Neoplasm Metastasis physiopathology pathology MeSH
- Tumor Cells, Cultured MeSH
- Neoplasm Proteins metabolism MeSH
- Breast Neoplasms metabolism MeSH
- Peptide Mapping MeSH
- Proteomics MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Comparative Study MeSH
We performed a 2-DE analysis of proteins of the newly established spontaneously immortalized clonal cell line EM-G3 derived from a primary lesion of infiltrating ductal breast carcinoma. EM-G3 cells may represent progenitors of the mammary epithelial cells spontaneously immortalized in early phase of cancerogenesis. We compared the protein profile of EM-G3 line with proteins from populations of normal mammary epithelial cells (NME), and determined the phenotype of both types of cells. NME cells are a mixture of both main cell types in breast epithelia, myoepithelial and luminal cells. The EM-G3 breast cancer cell line has a unique basal-like phenotype. We identified proteins that are differently expressed in these cells. Cytokeratin 16, cytokeratin 19, squamous cell carcinoma antigen 1, caphepsin B and caspase 14 were predominantly expressed by NME cells. Cytokeratin 13, isoelectric variant of annexin 5, isoelectric variant of chloride intracellular channel protein 1, glyoxalase 1 and glutamine synthetase were predominantly expressed by EM-G3 cells. The proteins up-regulated in EM-G3 cells may represent potential protein markers of mammary epithelial cells progenitors and may be important in early phase of carcinogenesis.
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Carcinoma, Ductal, Breast chemistry MeSH
- Phenotype MeSH
- Financing, Organized MeSH
- Stem Cells chemistry MeSH
- Humans MeSH
- Mammary Glands, Human cytology chemistry MeSH
- Cell Line, Tumor MeSH
- Neoplasm Proteins chemistry MeSH
- Breast Neoplasms chemistry MeSH
- Proteome chemistry MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Check Tag
- Humans MeSH
- Publication type
- Comparative Study MeSH
- MeSH
- Electrophoresis, Gel, Two-Dimensional * methods instrumentation utilization MeSH
- Biomedical Technology methods trends MeSH
- Mass Spectrometry * methods utilization MeSH
- Carcinoembryonic Antigen isolation & purification MeSH
- Coculture Techniques methods utilization MeSH
- Medical Oncology trends MeSH
- Humans MeSH
- Mammography methods utilization MeSH
- Mucins diagnostic use isolation & purification MeSH
- Biomarkers, Tumor isolation & purification MeSH
- Breast Neoplasms * diagnosis MeSH
- Mass Screening methods utilization MeSH
- Statistics as Topic MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Azacitidine pharmacology metabolism therapeutic use MeSH
- Drug Resistance, Neoplasm genetics MeSH
- Deoxycytidine pharmacology metabolism therapeutic use MeSH
- Epigenesis, Genetic drug effects MeSH
- Enzyme Inhibitors pharmacology metabolism therapeutic use MeSH
- Humans MeSH
- DNA Methylation * genetics drug effects MeSH
- Neoplasms drug therapy genetics MeSH
- Antimetabolites, Antineoplastic MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
