Cíl práce: Demonstrovat potenciální reverzibilitu ischemického selhání ledviny způsobené těsnou stenózou/uzávěrem renální arterie (RA) po úspěšné angioplastice u šesti nemocných. Materiál, metodika a výsledky: Pacientka č. 1: Žena, 34 let. Úplný akutní uzávěr obou RA, jedna přídatná RA je průchocbiá. Dialyzována dva týdny. Oboustranná PŤA se zavedením stentu obnovila ledvinnou funkci. Sérový kreatinin je stabilní kolem 120 μmol/l, sledována čtyři roky. Pacient č. 2: Muž, 70 let. Uzávěr tepny solitární ledviny. Dialyzován jeden týden. Rekanalizace RÄ se zavedením stentu obnovila funkci ledviny. Pacient umírá 13 dní po PTA na infarkt myokardu, funkce ledviny je normální. Pacient č. 3: Muž, 49 let. CHRI. Uzávěr tepny solitámí ledviny. Dialyzován 12 dní. Rekanalizace ledvinné tepny zavedením stentu obnovila fuiďcci ledviny. Sledován 18 měsíců: Sérový kreatinin 380 μmol/l. Pacientka č. 4: Žena, 72 let. Příbuzenská transplantace před 29 lety. Selhání Štěpu pro těsnou stenózu tepny. Dialyzována dva týdny. PTA obnovila funkci ledviny. Funkce štěpu je stabilní 12 měsíců, potom zánik funkce. Pacient č. 5: Muž, 47 let. Transplantace kadaverózní ledviny před dvěma roky. Selhání štěpu pro těsnou stenózu tepny. Intermitentně dialyzován devět mesicu. PTA obnovila funkci stepu. Sledován 42 měsíců, bez dialýzy, stabilní funkce štěpu. Pacientka Č. 6: Zena, 30 let. Druhá kadaverózní transplantace. Dva měsíce po transplantaci selhání štěpu pro ostiální zúžení tepny štěpu. Dialyzována jeden týden. PTA obnovila funkci štěpu. Sledování:: Funkce ledviny je 11 měsíců stabilní, poté selhává pro rejekci. Závěr : Zkušenost autorů potvrzuje, že ischemické selhání ledviny způsobené stenózou/okluzí RA muze být reverzibilní dokonce dny či týdny po vzniku stenózy/uzávěru a že i pozdní rekanalizace RA může být úspěšná.
Objective: To demonstrate the potential reversibility of ischaemic kidney failure caused by total or subtotal renal artery (RA) occlusion after delayed PTA/stenting in six patients. Material, methods and resiUts: Case 1: F age 34 years. Total acute occlusion of both RA, one polar artery patent. Dialy sed for two weeks. Bilateral RA PTA/stenting restored kidney function. F/U three years: Cr level 120 μmol/l. Case 2: M, age 70 years. Occlusion of a solitary kidney RA. Dialysed for one week. PTA/stenting of the occluded artery restored kidney function. The patient died two weeks later of MI, kidney function normal. Case 3: M, age 49 years. CRI. Occlusion of a solitary kidney artery. Dialysed for 12 days. Recanalisation of the occluded RA by stent implantation restored kidney function. F/U 18 months: Cr 380 μmol/l. Case 4: F, age 72 years. Living related donor transplantation 29 years . ago. Graft failure due to subtotal occlusion of RA. Dialysed for two weeks. PTA restored graft function. F/U: Good graft function for 12 months, then again graft failure. Case 5: M, age 47 years. Cadaverous transplantation two years ago. Graft failure due to subtotal occlusion of graft artery. Dialysed intermittently for 9 months. PTA restored kidney function. F/U 42 months: Dialysis free, graft function stable. Case 6: F, age 30 years. Second cadaverous transplantation. Two months after transplantation graft failure due to ostial RA stenosis. Dialysed for one week. PTA restored graft function. F/U: 11 months dialysis free, then again graft failure due to rejection. Conclusion: Authors experience confirms, that ischaemic kidney failure due to RA occlusion may be reversible even days to weeks after occlusion and that even delayed RA recanalisation should be attempted.
- MeSH
- Acute Kidney Injury MeSH
- Angioplasty, Balloon methods MeSH
- Humans MeSH
- Renal Artery Obstruction MeSH
- Kidney Transplantation MeSH
- Check Tag
- Humans MeSH
- Publication type
- Case Reports MeSH
- MeSH
- Cells, Cultured MeSH
- Composite Resins toxicity MeSH
- Dental Restoration, Permanent MeSH
- Publication type
- Comparative Study MeSH
Since 2009, the face transplant team at Brigham and Women's Hospital in Boston has performed 6 successful partial and full facial transplantations on carefully selected patients. The development of these techniques has led to a new era in facial reconstruction which now more correctly can be described as facial restoration. Besides the obvious facts of giving someone with a missing or severely disfigured face a new appearance, facial restoration has led to many other interesting observations in terms of immunologic models, airway functionality, sensory recovery and cerebral cortical functioning. In this article, we present an overview of our experience, and where we are today-also presenting some of the interesting avenues that have opened and will lead us further in the daunting experience of facial allotransplantation.
- Publication type
- Journal Article MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Hundred years ago, Sir Harold Gillies laid a foundation to the modern plastic surgery trying to reconstruct facial defects of severely disfigured soldiers of World War I. Some years later, Joseph Murray experimented with rejection of skin grafts aimed for treatment of burned patients who sustained their injuries on battlefields of World War II. In 1954, the acquired expertise and intensive research allowed him to perform the first successful kidney transplantation in the world at Peter Bent Brigham Hospital in Boston. For his achievements in organ transplantation he was awarded Nobel Prize in 1990. The face transplantation appears to be a natural evolution of the work of these two extraordinary plastic surgeons. The first case of partial face transplant from 2005 in France revealed the world that facial restoration by transplantation is superior to conventional reconstruction methods. Since 2009, our team has performed 7 cases of face transplantation at Brigham and Women's Hospital, which is to our best knowledge the largest living single center face transplant cohort in the world. In this article, we want to reflect on the experience with face transplantation at our institution from the past years. We aim to briefly review the key points of the know-how which was given to us from the care of these unique patients.
- Publication type
- Journal Article MeSH
- Review MeSH
- MeSH
- Animal Experimentation MeSH
- Radiation, Ionizing MeSH
- Bone Marrow metabolism radiation effects MeSH
- Rats MeSH
- Leukocytes metabolism radiation effects MeSH
- Nucleic Acids blood metabolism radiation effects MeSH
- Regeneration MeSH
- Spleen metabolism radiation effects MeSH
- Thymus Gland metabolism radiation effects MeSH
- Check Tag
- Rats MeSH
- MeSH
- Biomedical and Dental Materials MeSH
- Adult MeSH
- Humans MeSH
- Denture Design MeSH
- Orthotic Devices MeSH
- Prosthodontics MeSH
- Dental Restoration, Permanent MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
INTRODUCTION: This study aimed to identify the phytochemical constituents that could target gastric cancer in Kangai injection using a network pharmacology-based approach. METHODS: Protein-protein interactions (PPI), Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were conducted utilizing String and OmicShare tools. In the in vitro experiments, the related mRNA and protein levels were assessed via real-time quantitative polymerase chain reaction and Western blotting, respectively. Cell proliferation was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay. RESULTS: Kangai injection comprises several compounds, which target multiple substrates and pathways related to gastric cancer. The PPI and Gene Ontology analyses revealed that tumor necrosis factor (TNF) was a hub gene. KEGG pathway enrichment analysis indicated that the the TNF pathway was significantly enriched. Kangai injection decreased the mRNA levels of TNFR2, TRAF2, PI3K, AKT, and IκBα and inhibited the phosphorylation of PI3K, AKT, and IκBα phosphorylations. Kangai injection inhibited cell proliferation, while TNFR2 overexpression or treatment with the PI3K activator 740 Y-P partially restored it. CONCLUSION: Kangai injection operates through multiple targets and pathways in gastric cancer, with the TNFR2/PI3K/AKT/NF-κB pathway playing a crucial role in its mechanism against gastric cancer.
- MeSH
- Phosphatidylinositol 3-Kinases MeSH
- Humans MeSH
- Stomach Neoplasms * drug therapy genetics MeSH
- NF-KappaB Inhibitor alpha MeSH
- Proto-Oncogene Proteins c-akt MeSH
- Receptors, Tumor Necrosis Factor, Type II MeSH
- Network Pharmacology MeSH
- Tumor Necrosis Factor-alpha genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The first experiments on the use of wetland plants to treat wastewaters were carried out in the early 1950s by Dr. Käthe Seidel in Germany and the first full-scale systems were put into operation during the late 1960s. Since then, the subsurface systems have been commonly used in Europe while free water surface systems have been more popular in North America and Australia. During the 1970s and 1980s, the information on constructed wetland technology spread slowly. But since the 1990 s the technology has become international, facilitated by exchange among scientists and researchers around the world. Because of the need for more effective removal of ammonia and total nitrogen, during the 1990 s and 2000s vertical and horizontal flow constructed wetlands were combined to complement each other to achieve higher treatment efficiency. Today, constructed wetlands are recognized as a reliable wastewater treatment technology and they represent a suitable solution for the treatment of many types of wastewater.
- MeSH
- Biodegradation, Environmental MeSH
- Wetlands MeSH
- Waste Disposal, Fluid methods MeSH
- Water Movements MeSH
- Environmental Restoration and Remediation methods MeSH
- Water Pollution analysis statistics & numerical data MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
Experts in forensic anthropology and medicine have become gradually accustomed to examining components of the human body in the virtual workspace. While the computer-assisted approach offers numerous benefits, the interactions with digital three-dimensional biological objects are often problematic, particularly if conducted with mouse, keyboard and flat-panel screen. The study focusses on feasibility of a virtual reality (VR) system for virtual restoration of fragmentary skeletal remains. The VR system was confronted with three cases of fragmentary remains. The cases were reassembled manually by twenty participants using a HTC Vive headset combined with an in-house application A.R.T. The same task was performed using a CloudCompare software in conjunction with a desktop peripheral. The two systems were compared in terms of time efficiency, the geometric properties of the resulting restorations, and convenience of use. Restoration using the VR system took approximately half the time the desktop set-up did. The VR system also yielded a lower error rate when a severely fragmented skull was reassembled. Ultimately, although the efficiency of the reassembling was shown to be strongly dependent on the operator's experience, the use of the VR system balanced out the uneven levels of proficiency in computer graphics. The current generation of virtual reality headsets has a strong potential to facilitate and improve tasks relating to the virtual restoration of fragmented skeletal remains. A VR system offers an intuitive digital working environment which is less affected by an operator's computer skills and practical understanding of the technology than the desktop systems are.
- MeSH
- Adult MeSH
- Skull Fractures diagnostic imaging MeSH
- Humans MeSH
- Image Processing, Computer-Assisted MeSH
- Software MeSH
- Forensic Anthropology methods MeSH
- Wounds, Gunshot diagnostic imaging MeSH
- Feasibility Studies MeSH
- Body Remains * MeSH
- Virtual Reality * MeSH
- Imaging, Three-Dimensional MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
