Projekt má za cíl v preklinické části ověřit vliv přidání plazmy bohaté na trombocyty (Platelet-Rich Plasma; PRP), plazmy bohaté na fibrin (Platelet-Rich Fibrin; PRF), nebo derivátů sklovinné matrix (Emdogain; EMD) do kolagenní matrix na vznik keratinizované gingivy na zvířecím modelu. V klinické části studie budeme kombinovat PRP, PRF, nebo EMD s kolagenní matrix při krytí gingiválních recesů. Případný benefit této kombinované terapie může sloužit pro ostatní příbuzné medicínské obory, které využívají postupů regenerativní medicíny (ortopedie, kožní lékařství, maxilofaciální chirurgie, ORL, atd.).; The project aims to validate the effect of Platelet-Rich Plasma (PRP), Platelet-Rich Fibrin (PRF) and Enamel Matrix Derivate (Emdogain, EMD) with collagen matrix to increase the formation of keratinized gingiva in an animal model. A clinical study will be performed, where the combined therapy of PRP, PRF, or EMD with collagen matrix for coverage of gingival recession will be evaluated. The potential benefit of this combined therapy can be used for other related medical disciplines that use the practice of regenerative medicine (orthopedics, skin medicine, maxillofacial surgery, ENT, etc.).

• MeSH
• Extracellular Matrix MeSH
• Fibrin MeSH
• Gingiva MeSH
• Keratins MeSH
• Disease Models, Animal MeSH
• Mice MeSH
• Platelet-Rich Plasma MeSH
• Regenerative Medicine MeSH
• Gingival Recession MeSH
• Dental Enamel MeSH
• Check Tag
• Mice MeSH

• Conspectus
• Stomatologie
• NML Fields
• zubní lékařství
• biochemie
• NML Publication type
• závěrečné zprávy o řešení grantu IGA MZ ČR

Members of the galectin family of endogenous lectins are potent adhesion/growth-regulatory effectors. Their multifunctionality opens possibilities for their use in bioapplications. We studied whether human galectins induce the conversion of human dermal fibroblasts into myofibroblasts (MFBs) and the production of a bioactive extracellular matrix scaffold is suitable for cell culture. Testing a panel of galectins of all three subgroups, including natural and engineered variants, we detected activity for the proto-type galectin-1 and galectin-7, the chimera-type galectin-3 and the tandem-repeat-type galectin-4. The activity of galectin-1 required the integrity of the carbohydrate recognition domain. It was independent of the presence of TGF-β1, but it yielded an additive effect. The resulting MFBs, relevant, for example, for tumor progression, generated a matrix scaffold rich in fibronectin and galectin-1 that supported keratinocyte culture without feeder cells. Of note, keratinocytes cultured on this substratum presented a stem-like cell phenotype with small size and keratin-19 expression. In vivo in rats, galectin-1 had a positive effect on skin wound closure 21 days after surgery. In conclusion, we describe the differential potential of certain human galectins to induce the conversion of dermal fibroblasts into MFBs and the generation of a bioactive cell culture substratum.

• MeSH
• Extracellular Matrix metabolism   MeSH
• Fibroblasts metabolism   MeSH
• Galectin 1 metabolism   MeSH
• Galectin 3 metabolism   MeSH
• Galectin 4 metabolism   MeSH
• Galectins metabolism   MeSH
• Wound Healing MeSH
• Keratin-19 metabolism   MeSH
• Rats MeSH
• Humans MeSH
• Myofibroblasts metabolism   MeSH
• Tissue Engineering methods   MeSH
• Transforming Growth Factor beta1 metabolism   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Humans MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Previously, we found that treatment of cutaneous wounds with Atropa belladonna L. (AB) revealed shortened process of acute inflammation as well as increased tensile strength and collagen deposition in healing skin wounds (Gál et al. 2009). To better understand AB effect on skin wound healing male Sprague-Dawley rats were submitted to one round full thickness skin wound on the back. In two experimental groups two different concentrations of AB extract were daily applied whereas the control group remained untreated. For histological evaluation samples were removed on day 21 after surgery and stained for wide spectrum cytokeratin, collagen III, fibronectin, galectin-1, and vimentin. In addition, in the in vitro study different concentration of AB extract were used to evaluate differences in HaCaT keratinocytes proliferation and differentiation by detection of Ki67 and keratin-19 expressions. Furthermore, to assess ECM formation of human dermal fibroblasts on the in vitro level fibronectin and galectin-1 were visualized. Our study showed that AB induces fibronectin and galectin-1 rich ECM formation in vitro and in vivo. In addition, the proliferation of keratinocytes was also increased. In conclusion, AB is an effective modulator of skin wound healing. Nevertheless, further research is needed to find optimal therapeutic concentration and exact underlying mechanism of action.

• MeSH
• Atropa belladonna chemistry   MeSH
• Time Factors MeSH
• Extracellular Matrix metabolism   drug effects   MeSH
• Fibroblasts metabolism   pathology   drug effects   MeSH
• Fibronectins metabolism   MeSH
• Galectin 1 metabolism   MeSH
• Wound Healing drug effects   MeSH
• Keratin-19 metabolism   MeSH
• Keratinocytes metabolism   pathology   drug effects   MeSH
• Collagen Type III metabolism   MeSH
• Rats MeSH
• Cells, Cultured MeSH
• Skin metabolism   pathology   drug effects   injuries   MeSH
• Humans MeSH
• Disease Models, Animal MeSH
• Wounds, Penetrating drug therapy   metabolism   pathology   MeSH
• Rats, Sprague-Dawley MeSH
• Plant Extracts pharmacology   chemistry   isolation & purification   MeSH
• Solvents chemistry   MeSH
• Vimentin metabolism   MeSH
• Water chemistry   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Humans MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

The organic components of bones and other mineralized tissues have a high impact on the organization and deposition of calcium, and consequently influence the mechanical properties of those tissues. The extractable proteins of avian eggshells have been studied extensively and many of them have been identified; insoluble (non-extractable) proteins have been sparsely studied, however. In the work discussed in this paper we studied EDTA-insoluble proteins by gradual decalcification of eggshell with EDTA. The insoluble proteinaceous films were chemically treated with cyanogen bromide and the mixtures of large fragments obtained were gradually precipitated with salt. The separated fractions were digested with trypsin and analyzed by HPLC-MS-MS (ion trap mass spectrometer). Analysis of the entire eggshell matrix (without precipitation steps) only enabled 6 proteins to be determined (ovocalyxins 32 and 36, ovocleidin 17 and 116, clusterin, and ovalbumin). Pretreatment of the individual eggshell layers and gradual precipitation with salt markedly increased the number of proteins identified - 28 proteins were determined. We identified for the first time collagens I (two chains) and III in the eggshell matrix, and Kunitz-like protease inhibitor as a major shell matrix protein. Besides the above mentioned proteins we can also mention EDIL3, fibronectin, sulfhydryl oxidase, tubulin alpha 1, lysozyme, Dickkopf-related protein 3, keratins, and ovotransferrin. The relative abundances of proteins in all eggshell layers were determined using the exponentially modified protein abundance index (emPAI). In the cuticle layer seven proteins were identified, whereas 16 proteins were described in the palisade layer and 23 in the mammillary layer.

• MeSH
• Spectrometry, Mass, Electrospray Ionization MeSH
• Chickens MeSH
• Solubility MeSH
• Egg Shell chemistry   MeSH
• Egg Proteins analysis   MeSH
• Chromatography, High Pressure Liquid MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Macrofibrils, the main structural features within the cortical cells of mammalian hair shafts, are long composite bundles of keratin intermediate filaments (KIFs) embedded in a matrix of keratin-associated proteins. The KIFs can be helically arranged around the macrofibril central axis, making a cylinder within which KIF helical angle relative to macrofibril axis increases approximately linearly from macrofibril centre to edge. Mesophase-based self-assembly has been implicated in the early formation of macrofibrils, which first appear as liquid-crystal tactoids in the bulb of hair follicles. Formation appears to be driven initially by interactions between pre-keratinized KIFs. Differences in the nature of these KIF-KIF interactions could result in all macrofibrils being internally twisted in a single handedness, or a 50:50 mixture of handedness within each cortical cell. We data-mined 41 electron tomograms containing three-dimensional macrofibril data from previously published studies of hair and wool. In all 644 macrofibrils examined we found that within each tomogram all macrofibrils had the same handedness. We concluded that earlier reports of left- and right-handed macrofibrils were due to artefacts of imaging or data processing. A handedness marker was used to confirm (using re-imaged sections from earlier studies) that, in both human and sheep, all macrofibrils are left-handed around the macrofibril axis. We conclude that this state is universal within mammalian hair. This also supports the conclusion that the origin of macrofibril twist is the expression of chiral twisting forces between adjacent KIFs, rather than mesophase splay and bending forces relaxing to twisting forces acting within a confined space.

• MeSH
• Cytoskeleton chemistry   ultrastructure   MeSH
• Intermediate Filaments chemistry   ultrastructure   MeSH
• Keratins chemistry   ultrastructure   MeSH
• Humans MeSH
• Sheep genetics   MeSH
• Electron Microscope Tomography MeSH
• Hair chemistry   ultrastructure   MeSH
• Wool chemistry   ultrastructure   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Double-label immunofluorescence was used to monitor basement-membrane composition and integrity in 22 human colon polyps, 36 adenocarcinomas and 2 metastases. Cryostat sections were stained with polyclonal anti-laminin anti-serum combined with monoclonal antibodies (MAbs) to all major basement-membrane components (laminin, entactin/nidogen, collagen type IV and large heparan sulfate proteoglycan), as well as to keratin 8. In all adenocarcinomas, including mucinous, basement membranes were altered more at the invasive front than in the parenchyma. The degree of this alteration was inversely correlated with the level of tumor differentiation. An uncoordinated loss of basement membrane components (dissociation of markers), previously described by us in rat colon adenocarcinomas, was also found in human tumors. In the great majority of adenocarcinomas a pronounced stromal reaction was seen. It was manifested by the presence of fibrillar deposits of basement-membrane components, mainly of collagen type IV and/or heparan sulfate proteoglycan. This reaction was never observed in polyps and may be derived from myofibroblasts reported to accumulate in colon cancer stroma. The combined use of antibodies to basement-membrane components and to a specific keratin may constitute an adequate immunohistochemical test for the presence of invasion, and may be useful in the histologic analysis of polyps, especially in dubious cases.

• MeSH
• Adenocarcinoma metabolism   MeSH
• Basement Membrane metabolism   MeSH
• Extracellular Matrix Proteins metabolism   MeSH
• Fluorescent Antibody Technique MeSH
• Heparan Sulfate Proteoglycans MeSH
• Heparitin Sulfate metabolism   MeSH
• Keratins metabolism   MeSH
• Collagen metabolism   MeSH
• Colon metabolism   MeSH
• Laminin metabolism   MeSH
• Humans MeSH
• Lymphatic Metastasis MeSH
• Membrane Glycoproteins metabolism   MeSH
• Antibodies, Monoclonal MeSH
• Intestinal Polyps metabolism   MeSH
• Proteoglycans metabolism   MeSH
• Intestinal Neoplasms metabolism   MeSH
• Blotting, Western MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH

Cholesteatom je charakterizován přítomností dlaždicobuněčného epitelu ve středoušní dutině; skládá se z buněčné matrix a rohovějící vrstvy epitelu. Je rozlišován získaný a kongenitální typ. Patogeneze cholesteatomu není stále vyřešena. Jednou z teorií vzniku kongenitálního cholesteatomu je Michaelsova teorie epidermoidních ložisek (formací). Epidermoidní ložiska (EF) jsou lokalizována ve stejném místě jako většina kongenitálních cholesteatomů v přední horní části středoušní dutiny. V naší studii bylo vyšetřeno 92 spánkových kostí, u 16 (17 %) z nich byla nalezena epidermoidní ložiska. Celkem bylo identifikováno 39 epidermoidních formací v 16 spánkových kostech, tedy byla zjištěna přítomnost více epidermoidních formací v některých spánkových kostech. V jedné kosti jich bylo nalezeno dokonce sedm. Bylo možné pozorovat různá uspořádání epidermoidních formací: 21krát prominující typ, 13krát sférický smíšený typ, 4krát subepiteliální, invertovaný typ a jedenkrát intraepiteliální typ. Celkem 21 epidermodiních formací (53,8 %) bylo lokalizováno v nejtypičtějším místě, v přední horní části středoušní dutiny. Gestační věk jedinců všech vyšetřených spánkových kostí kolísal od 13. do 42. týdne. Nenalezli jsme epidermoidní formace u plodů starších než 33. týden gestace.

Cholesteatoma is an accumulation of stratified squamous epithelium in the middle ear, comprising both living cellular matrix and the dead keratinous layer. There are two types of cholesteatoma acquired and congenital. The pathogenesis of cholesteatoma is still not well understood. One theory of development of congenital cholesteatoma is Michaels' theory of epidermoid formation. The epidermoid foci are at the same site asmany congenital cholesteatomas, i.e. in the anterior superior part of the middle ear. In our study 92 foetal temporal bones were examined and epidermoid formation was found in 16 i.e. 17%. We found 39 epidermoid formations in 16 temporal bones, thus there were several epidermoid foci, in one temporal bone (maximum 7). The epidermoid formations presented several configurations: 21 superficial, 13 spherical and mixed, 4 elongated into the subepitheliumand one intraepithelial. Twenty - one epidermoid formations (53.8%) were at the most typical site e.g. in the anterior superior part of the middle ear. The gestational age of the foetuses were from 13 to 42 weeks.We did not find any epidermoid formation after the 33rd week of gestation.

• MeSH
• Cholesteatoma, Middle Ear genetics   pathology   MeSH
• Keratins MeSH
• Neoplasms, Glandular and Epithelial MeSH
• Fetal Diseases MeSH
• Temporal Bone anatomy & histology   pathology   MeSH
• Ear, Middle anatomy & histology   pathology   MeSH

Cysteine dioxygenase (CDO, EC 1.13.11.20) catalyses the oxygenation of cysteine to cysteine sulphinic acid leading to the production of sulphite, sulphate and taurine as the final metabolites of cysteine catabolism. Keratinolytic fungi secrete sulphite and sulphate to reduce disulphide bridges in host tissue keratin proteins as the first step of keratinolysis. In the present study, we describe the identification of cDNA, as well as expression and characterisation of recombinant CDO protein from Trichophyton mentagrophytes. The cDNA was amplified using primers designed on the basis of high conservancy CDO regions identified in other fungi. PCR product was cloned and sequenced. Recombinant CDO was expressed in Escherichia coli, and affinity purified and identified by matrix-assisted laser desorption/ionization - time-of-flight mass spectrometry (MALDI-TOF MS). Enzyme activity was assayed by monitoring the production of cysteine sulphinate using mass spectrometry. The Cdo cDNA encodes for a protein consisting of 219 amino acids. Recombinant CDO protein C-terminally fused with a His tag was purified by affinity chromatography. The CDO purified under native condition was proved to be enzymatically active. Protein identity was confirmed by MALDI-TOF MS. Comparison of cDNA sequence with those identified in other fungi revealed significant homology. Identification of T. mentagrophytes CDO provides indispensable tools for future studies of dermatophyte pathogenicity and development of new approaches for prevention and therapy.

• MeSH
• Chromatography, Affinity MeSH
• Cysteine analogs & derivatives   metabolism   MeSH
• Cysteine Dioxygenase genetics   isolation & purification   MeSH
• DNA, Fungal chemistry   genetics   MeSH
• Escherichia coli genetics   MeSH
• Gene Expression MeSH
• Cloning, Molecular MeSH
• DNA, Complementary genetics   MeSH
• Humans MeSH
• Molecular Sequence Data MeSH
• Recombinant Proteins genetics   isolation & purification   metabolism   MeSH
• Amino Acid Sequence MeSH
• Sequence Analysis, DNA MeSH
• Sequence Homology, Amino Acid MeSH
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
• Trichophyton enzymology   genetics   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Úvod: Proliferativní nádorové markery a matrixové metaloproteinázy a jejich tkáňové inhibitory odráží vlastnosti malignity a mohou být užitečné při hodnocení prognózy jaterních metastáz kolorektálního karcinomu (CLM). Přesto není mnoho známo o jejich fyziologických funkcích a chování během regenerace a hojení jaterního parenchymu po jaterním výkonu pro malignitu. Metody: Do studie byli zahrnuti pacienti, kteří byli operováni v našem zařízení v období od 11/2002–12/2004 a podstoupili následující výkony: skupina (kontrolní) A: 22 pacientů s tříselnou kýlou; skupina B: 26 pacientů s benigní ložiskovou jaterní lézí; skupina C: 30 pacientů s CLM léčenou radiofrekvenční ablací; skupina D: 41 pacientů s CLM léčených radikální chirurgickou léčbou a skupina E: 22 pacientů s inoperabilním CLM, kteří podstoupili explorativní laparotomii. U všech pacientů byly stanoveny předoperační a pooperační sérové hladiny CEA, CA19-9, TK, TPA, TPS, MMP-2, MMP-9, TIMP-1 a TIMP-2. Výsledky: Srovnání kontrolní skupiny A se skupinou B ukázalo významné rozdíly v předoperačních sérových hladinách MMP-2 a -9 a TIMP-2 a pooperačních sérových hladinách TPS, TK, MMP-2 a TIMP-2. Srovnání kontrolní skupiny A se skupinou C vykazovalo statisticky významné rozdíly v předoperačních sérových hladinách TPA, TPS, MMP- 2, TIMP-2, CEA a CA19-9 a pooperačních sérových hladinách TK, TPA, TPS, TIMP-1 a -2, CEA a CA19-9. Srovnání kontrolní skupiny A se skupinou D demonstrovalo rozdíly v předoperačních sérových hladinách TPA, TPS, MMP-2 a -9, TIMP-1 a -2, CEA a CA19-9 a pooperačních sérových hladinách TK, TPA, TPS, MMP-9, TIMP-1 a -2, CEA a CA19-9. Srovnání kontrolní skupiny A se skupinou E vykazovalo významné rozdíly v předoperačních sérových hladinách TK, TPA, TPS, MMP-2 a -9, TIMP-1 a -2, CEA a CA19-9 a pooperačních sérových hladinách TK, TPA, TPS, MMP-2, TIMP-1 a -2, CEA a CA19-9. Závěr: Retrospektivní analýza sérových hodnot různých nádorových markerů, metaloproteináz a jejich tkáňových inhibitorů před chirurgickými výkony a po nich ukázala, že sérové koncentrace těchto ukazatelů jsou významně ovlivňovány remodelací tkáně a procesem hojení. Výsledky této studie ukazují, že uvedené markery nejsou spolehlivé pro stanovení prognózy nemocných po chirurgické léčbě CLM.

Introduction: A proliferative tumour markers and matrix metalloproteinases and their tissue inhibitors reflect the features of malignancy and they have a role in prognosis prediction in patients with colorectal metastases (CLM) in the liver. Their physiological functions during liver regeneration is not comptelety known. Methods: Only patients those had been operated in our department between 11/2002 and 12/2004 were included. They underwent the following surgical procedures: Group (control) A: 22 patients with groin hernias, Group B: 26 patients with benign liver lesions, Group C: 30 CLM patients were treated by radiofrequency ablation, Group D: 41 patients with CLM those underwent radical liver surgery and Group E: 22 patients with inoperable CLM those underwent explorative laparotomy only. The preoperative and postoperative serum levels of CEA, CA19-9, K, TPA, TPS, MMP-2, MMP-9, TIMP-1 and TIMP-2 were measured. Results: We proved a asignificat differences of serum levels MMP-2, -9; TIMP-2 and TPS, TK, MMP-2, TIMP-2 in a preoperative and postoperative settings respectively between control Group A and Group B. The comparison of control Group A and Group C displayed a significant differences in the preoperative serum levels of TPA, TPS, MMP-2, TIMP-2, CEA and CA19-9 and postoperative serum levels of TK, TPA, TPS, TIMP-1, -2, CEA, CA19-9 respectively. The comparison of control Group A and Group D demonstrated a significant diferences in the preoperative serum levels of TPA, TPS, MMP-2 and -9, TIMP-1, -2, CEA, CA19-9 and postoperative serum levels of TK, TPA,TPS, MMP-9, TIMP-1, -2, CEA, CA19-9 respectively. The comparison of control Group A with Group E proved a significant differences in the preoperative serum levels of TK, TPA, TPS, MMP-2 and -9, TIMP-1, -2, CEA, CA19-9 and postoperative serum levels of TK, TPA, TPS, MMP-2, TIMP-1, -2, CEA, CA19-9 respectively. Conclusion: The retrospective analysis of serum matrix metalloproteinases and their tissues inhibitors in differenet group of patients has shown that serum levels of tumour markers are influenced significantly due to the regeneration and remodelling of healing tissues. The study findings support a critical view how to use these tumour markers for disease prognosis prediction in patients after surgical procedure for CLM.

• Keywords
• nádorové markery, jaterní chirurgie, průběh nádorových markerů, jaterní resekce, prognostické faktory, jaterní metastázy, kolorektální karcinom, časná recidiva, benigní jaterní choroby,
• MeSH
• Antigens, Tumor-Associated, Carbohydrate blood   MeSH
• Digestive System Surgical Procedures methods   MeSH
• Adult MeSH
• Financing, Organized MeSH
• Hepatectomy MeSH
• Hernia, Inguinal surgery   MeSH
• Carcinoembryonic Antigen blood   MeSH
• Catheter Ablation MeSH
• Keratins blood   MeSH
• Colorectal Neoplasms complications   MeSH
• Middle Aged MeSH
• Humans MeSH
• Metalloproteases blood   MeSH
• Neoplasm Metastasis pathology   MeSH
• Adolescent MeSH
• Young Adult MeSH
• Biomarkers, Tumor blood   MeSH
• Liver Neoplasms surgery   pathology   secondary   MeSH
• Predictive Value of Tests MeSH
• Prognosis MeSH
• Radiofrequency Ablation MeSH
• Retrospective Studies MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Statistics as Topic MeSH
• Tissue Inhibitor of Metalloproteinases blood   MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Adolescent MeSH
• Young Adult MeSH
• Male MeSH
• Aged, 80 and over MeSH
• Aged MeSH
• Female MeSH

Colorectal carcinoma (CRC) is a disease that causes significant morbidity and mortality worldwide. To improve treatment, new biomarkers are needed to allow better patient risk stratification in terms of prognosis. This study aimed to clarify the prognostic significance of colonic-specific transcription factor special AT-rich sequence-binding protein 2 (SATB2), cytoskeletal protein cytokeratin 7 (CK7), and immune checkpoint molecule programmed death-ligand 1 (PD-L1). We analyzed a cohort of 285 patients with surgically treated CRC for quantitative associations among the three markers and five traditional prognostic indicators (i.e., tumor stage, histological grade, variant morphology, laterality, and mismatch-repair/MMR status). The results showed that loss of SATB2 expression had significant negative prognostic implications relative to overall survival (OS) and cancer-specific survival (CSS), significantly shortened 5 years OS and CSS and 10 years CSS in patients with CRC expressing CK7, and borderline insignificantly shortened OS in patients with PD-L1 + CRC. PD-L1 showed a significant negative impact in cases with strong expression (membranous staining in 50-100% of tumor cells). Loss of SATB2 was associated with CK7 expression, advanced tumor stage, mucinous or signet ring cell morphology, high grade, right-sided localization but was borderline insignificant relative to PD-L1 expression. CK7 expression was associated with high grade and SATB2 loss. Additionally, a separate analysis of 248 neoadjuvant therapy-naïve cases was performed with mostly similar results. The loss of SATB2 and CK7 expression were significant negative predictors in the multivariate analysis adjusted for associated parameters and patient age. In summary, loss of SATB2 expression and gain of CK7 and strong PD-L1 expression characterize an aggressive phenotype of CRC.

• MeSH
• B7-H1 Antigen genetics   metabolism   MeSH
• Keratin-7 genetics   MeSH
• Colorectal Neoplasms * pathology   MeSH
• Humans MeSH
• Biomarkers, Tumor metabolism   MeSH
• Prognosis MeSH
• Transcription Factors genetics   MeSH
• Matrix Attachment Region Binding Proteins * genetics   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH