186 s.
sv.
- MeSH
- Genes MeSH
- Nucleic Acids MeSH
- Protein Biosynthesis MeSH
- Publication type
- Periodical MeSH
- Conspectus
- Biochemie. Molekulární biologie. Biofyzika
- NML Fields
- biochemie
- genetika, lékařská genetika
Nanofibrous materials produced from natural polymers have wide range of potential uses in regenerative medicine. This paper focuses on preparation of nanofibrous layers produced from intentionally hydrophobized derivatives of hyaluronan, which is known for its ability to promote wound healing. This structural modification of hyaluronan expands the range of potential uses of this promising material, which is otherwise limited due to the hydrophilic nature of hyaluronic acid. The aim of this research was preparation of nanofibrous material that would retain its fibrous structure and dimensional stability even after getting into contact with an aqueous medium, which is impossible to achieve with layers composed solely of native hyaluronan. As a result, such material would be able to retain its breathability and good mechanical properties when both dry and wet. Furthermore, all prepared materials were proved non-toxic for cells. This self-supporting nanofibrous matrix can be used as a scaffold, or porous wound dressing.
- Publication type
- Journal Article MeSH
Here we present a new effective antibacterial material suitable for a coating, e.g., surface treatment of textiles, which is also time and financially undemanding. The most important role is played by hydrophobic carbon quantum dots, as a new type of photosensitizer, produced by carbonization of different carbon precursors, which are incorporated by swelling from solution into various polymer matrices in the form of thin films, in particular polyurethanes, which are currently commercially used for industrial surface treatment of textiles. The role of hydrophobic carbon quantum dots is to work as photosensitizers upon irradiation and produce reactive oxygen species, namely singlet oxygen, which is already known as the most effective radical for elimination different kinds of bacteria on the surface or in close proximity to such modified material. Therefore, we have mainly studied the effect of hydrophobic carbon quantum dots on Staphylococcus aureus and the cytotoxicity tests, which are essential for the safe handling of such material. Also, the production of singlet oxygen by several methods (electron paramagnetic spectroscopy, time-resolved near-infrared spectroscopy), surface structures (atomic force microscopy and contact angle measurement), and the effect of radiation on polymer matrices were studied. The prepared material is easily modulated by end-user requirements.
- MeSH
- Anti-Bacterial Agents chemistry MeSH
- Biofilms MeSH
- Coated Materials, Biocompatible chemistry MeSH
- Smart Materials chemistry MeSH
- Photosensitizing Agents chemistry MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Quantum Dots chemistry MeSH
- Mice MeSH
- Surface Properties MeSH
- Reactive Oxygen Species metabolism MeSH
- Singlet Oxygen chemistry MeSH
- Staphylococcus aureus MeSH
- Carbon chemistry MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
