- MeSH
- Autoimmune Diseases therapy MeSH
- Cell Differentiation MeSH
- Phenotype MeSH
- Immunosuppressive Agents MeSH
- Humans MeSH
- Mesenchymal Stem Cells cytology metabolism MeSH
- Disease Models, Animal MeSH
- Mice MeSH
- Neoplasms therapy MeSH
- Mesenchymal Stem Cell Transplantation methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
326 s. : il.
Mezenchymálních kmenové buňky (MSC) jsou využívány k regeneraci poškozené mezenchymální tkáně. Jsou schopny se diferencovat ve zralé buňky kostní, chrupavčité, nervové, svalové či vazivové. Jejich účinek spočívá jednak v náhradě poškozené tkáně, ale také v parakrinním působení bioaktivních molekul. Autoři prezentují literární přehled recentního výzkumu MSC v oblasti otorinolaryngologie. V současnosti je výzkum zaměřen především na oblast fonochirurgie, percepční nedoslýchavosti a rekonstrukci rozsáhlých defektů kostních, chrupavčitých a měkkých tkání oblasti hlavy a krku. Také v České republice probíhá výzkum využití MSC v otorinolaryngologii. Prioritní oblastí je náhrada kostní tkáně, výzkum je zaměřen na terapii pooperačních defektů v oblasti spánkové kosti.
Multipotent mesenchymal stromal cells (MSC) are used for regeneration of injured mesenchymal tissue due to their self-renewal capacity and ability to differentiate into cells of mesenchymal tissue (bone, cartilage, muscle, fat, nerve or fibrous tissues). They regenerate various tissues through self-renewal, differentiation capacity, immune modulation and secretion of bioactive molecules. Authors present a review of MSC applications in otorhinolaryngology. The major interest is focused on phonosurgery, sensorineural deafness and reconstruction of large tissue defects with bone, cartilage or soft tissue replacement. The MSCs research in Czech otorhinolaryngology is focused on bone tissue replacement. Current point of interest is the temporal bone and treatment of bone defects after middle ear surgery.
- MeSH
- Cell- and Tissue-Based Therapy methods trends utilization MeSH
- Hematopoietic Stem Cells cytology classification MeSH
- Myocytes, Cardiac cytology transplantation MeSH
- Culture Techniques methods trends utilization MeSH
- Humans MeSH
- Mesenchymal Stem Cells * cytology immunology classification MeSH
- Heart Diseases therapy MeSH
- Osteogenesis Imperfecta etiology therapy MeSH
- Cell Plasticity * physiology genetics immunology MeSH
- Connective Tissue immunology growth & development transplantation MeSH
- Regenerative Medicine * classification methods trends MeSH
- Statistics as Topic MeSH
- Mesenchymal Stem Cell Transplantation methods trends utilization MeSH
- Stem Cell Research MeSH
- Check Tag
- Humans MeSH
Mezenchymální stromální buňky (mesenchymal stromal cells – MSC) jsou heterogenní populací kmenových buněk, které lze izolovat z mnoha různých tkání. MSC mohou diferencovat do buněk mezodermální linie (adipocytů, chondrocytů a osteoblastů) i ostatních zárodečných linií. MSC jsou schopny interagovat s buňkami vrozené i získané imunity, a navozovat tak modulaci ně kte rých funkcí imunitního systému. Po in vivo podání mohou MSC vyvolat toleranci a migrovat do míst poškozených tkání, kde mohou tlumit vznik prozánětlivých cytokinů a podporovat přežití poškozených buněk. Tato práce shrnuje výsledky experimentů s různými zdroji MSC a dále možnosti jejich izolace a kultivace vzhledem k požadavkům správné klinické praxe (good manufacture practice – GMP).
Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells that can be isolated from many adult tissues. They can differentiate into cells of the mesodermal lineage, such as adipocytes, osteocytes and chondrocytes, as well as cells of other embryonic lineages. MSCs can interact with cells of both the innate and adaptive immune systems, leading to the modulation of several effector functions. After in vivo administration, MSCs induce peripheral tolerance and migrate to injured tissues, where they can inhibit the release of pro-infl ammatory cytokines and promote the survival of damaged cells. This article recapitulates experimental data of MSC sources and mechanisms of MSC- isolation and cultivation according to GMP (good manufacture practice) standards.
- MeSH
- Cell Culture Techniques methods standards utilization MeSH
- European Union MeSH
- Clinical Trials, Phase II as Topic MeSH
- Bone Marrow immunology MeSH
- Humans MeSH
- Mesenchymal Stem Cells cytology immunology classification MeSH
- Cordocentesis utilization MeSH
- Flow Cytometry methods utilization MeSH
- Guidelines as Topic MeSH
- Statistics as Topic MeSH
- Blood Platelets cytology immunology MeSH
- Check Tag
- Humans MeSH
- Geographicals
- Czech Republic MeSH
PURPOSE OF THE STUDY Many congenital and acquired disorders as well as sequelae of injury are associated with articular cartilage degenera- tion, which adversely affects the patient's quality of life. The currently used cell therapy with cultured chondrocytes has its disadvantages due to a process of de-differentiation of chondrocytes during cultivation. We believe that the mesenchymal stem cell therapy offers a new treatment options. MATERIAL AND METHODS The adult mesenchymal stem cells (MSCs) for chondrocyte differentiation are usually obtained from bone marrow mesen- chymal stem cells (BMSCs). In this study these cells were compared with mesenchymal stem cells derived from adipose tissue (AMSCs). The aim of the study was to verify the ability of human BMSCs and AMSCs to differentiate into chondro- cytes in vitro in the presence or absence of transforming growth factor beta (TGF-ß1). Human BMSCs and AMSCs were collected from healthy donors during orthopaedic surgeries, in vitro cultured in three passages to obtain the required quan- tity of cells. A pellet culture system was used for chondrocyte differentiation. RESULTS At 21 days of cultivation, cell aggregates grown in the chondrogenic medium were larger than those cultured in the con- trol medium. Both the BMSCs and AMSCs pellet cultures showed spontaneous chondrogenesis. Histological staining with haematoxylin and eosin and Masson's trichrome stains, as well as immunohistochemical staining to detect type II collagen revealed no apparent differences between the pellet cultures with TGF-ß1 presence and those without it. The real-time RT-PCR detected expression of the type II collagen gene in all tested cultures. In the BMSCs pellet culture only, TGF-ß1 presence resulted in a decrease in type I collagen mRNA levels and in an increase in type II collagen mRNA values. DISCUSSION Our results showed an in vitro chondrogenic potential of mature human mesenchymal stem cells derived from both bone marrow and adipose tissue. In agreement with the relevant literature data, we suggest that both cell types have an equal prospect for use in cartilage tissue engineering. Key words: bone marrow, adipose tissue, transforming growth factor beta, cartilage, pellet culture system.
- MeSH
- Cell Differentiation MeSH
- Bone Marrow Cells cytology MeSH
- Chondrocytes cytology MeSH
- Chondrogenesis physiology MeSH
- Adult MeSH
- Financing, Organized MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Mesenchymal Stem Cells cytology MeSH
- Transforming Growth Factor beta1 pharmacology MeSH
- Adipose Tissue cytology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Publication type
- Comparative Study MeSH
