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Recently, we have established that slow soleus (SOL) and fast extensor digitorum longus (EDL) muscles of euthyroid (EU) Lewis rats posses the same proportions between their four myosin heavy chain (MyHC) mRNAs, protein isoforms and fiber types as determined by real time RT-PCR, SDS-PAGE and 2-D stereological fiber type analysis, respectively. In the present paper we investigated if these proportions are maintained in adult Lewis rats with hyperthyroid (HT) and hypothyroid (HY) status. Although HT and HY states change MyHC isoform expression, results from all three methods showed that proportion between MyHC mRNA-1, 2a, -2x/d, -2b, protein isoforms MyHC-1, -2a, -2x/d, -2b and to lesser extent also fiber types 1, 2A, 2X/D, 2B were preserved in both SOL and EDL muscles. Furthermore, in the SOL muscle mRNA expression of slow MyHC-1 remained up to three orders higher compared to fast MyHC transcripts, which explains the predominance of MyHC-1 isoform and fiber type 1 even in HT rats. Although HT status led in the SOL to increased expression of MyHC-2a mRNA, MyHC-2a isoform and 2A fibers, it preserved extremely low expression of MyHC-2x and -2b mRNA and protein isoforms, which explains the absence of pure 2X/D and 2B fibers. HY status, on the other hand, almost completely abolished expression of all three fast MyHC mRNAs, MyHC protein isoforms and fast fiber types in the SOL muscle. Our data present evidence that a correlation between mRNA, protein content and fiber type composition found in EU status is also preserved in HT and HY rats.

Článek

Genetická příčina hyperplázie hypofýzy u chlapce s deficitem růstového hormonu a centrální hypotyreózou

Bulletin dětské endokrinologie. 2013 ; 4 (1) : 5-8.

Medvik
Zdroj

MeSH
anamnéza MeSH
genetické testování MeSH
hyperplazie * diagnóza genetika MeSH
hypofýza * patofyziologie MeSH
hypotyreóza diagnóza genetika MeSH
lidé MeSH
mutace MeSH
předškolní dítě MeSH
růstový hormon nedostatek terapeutické užití MeSH
thyroxin MeSH
transkripční faktor Pit-1 * MeSH
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lidé MeSH
mužské pohlaví MeSH
předškolní dítě MeSH
Publikační typ
kazuistiky MeSH
práce podpořená grantem MeSH

Článek

Kim, B.W., Bianco, A.C.: Pro někoho nemusí být substituční léčba L-tyroxinem dostatečná: genetické údaje

Kalvachová, Blanka
Autor Kalvachová, Blanka

Revue endokrinologie. Supplementum. 2011 ; 5 (1) : 5-6.

Medvik
Zdroj

MeSH
hormonální substituční terapie MeSH
hypotyreóza farmakoterapie genetika MeSH
jodidperoxidasa genetika MeSH
lidé MeSH
thyroxin terapeutické užití MeSH
trijodthyronin terapeutické užití MeSH
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lidé MeSH
Publikační typ
souhrny MeSH

Článek

Glycerol-3-phosphate dehydrogenase expression and oxygen consumption in liver mitochondria of female and male rats with chronic alteration of thyroid status

Hormone and Metabolic Research. 2011 ; 43 (1) : 43-47.

Horm Metab Res
ISSN 1439-4286
Medvik
Zdroj

In our chronic experiments (over several months), the activity and protein amount of glycerol-3-phosphate dehydrogenase (GPDH) in mitochondria isolated from the liver of adult male and female inbred Lewis strain euthyroid (EU), hyperthyroid (TH), and hypothyroid (HY) rats were analyzed by biochemical and Western blot methods. The TH status was induced by intraperitoneal injections of 3,3',5-triiodo- L-thyronine and the HY status with 0.05% solution of methimazole in drinking water. The TH status led to a significant increase and the HY status to a significant decrease of enzyme activity and protein amount in both male and female animals. These changes were, however, more pronounced in females. The EU and TH female rats also showed a significantly higher activity and the TH female rats showed also a significantly higher enzyme amount in comparison with males, while the HY rats showed low levels in both sexes. The glycerol-3-phosphate-dependent oxygen consumption of freshly isolated rat liver mitochondria from the TH animals was higher in comparison with the EU animals and it was activated by idebenone, a synthetic analogue of coenzyme Q, in both the EU and TH rats. Measurements of serum thyroid hormone levels and analysis of anatomical parameters (relative heart and thyroid gland weights) confirmed that our procedures inducing the TH and HY states are efficient and reliable and that determination of GPDH can serve as an additional criterion for the evaluation of the thyroid hormone status.