dnešní době jsme zahlceni informacemi o tom, jak správně žít, budovat vztahy a pracovat na sobě. Avšak nadměrná teoretická příprava často vede k informačnímu přetížení a paralýze místo skutečné změny. Vztahy nevyžadují další kurzy či rady, ale odvahu jednat a žít přítomností. Sebepoznání samo o sobě nestačí – klíčové je sebepřijetí a integrace poznatků do života. Autor upozorňuje na past nekonečné přípravy, která brání prožívání skutečných vztahů, a zdůrazňuje, že vztahová spokojenost pramení z autenticity a ochoty se vztahům aktivně věnovat, nikoli z hledání dokonalosti.
Nowadays, we are overwhelmed with information on how to live correctly, build relationships, and work on ourselves. However, excessive theoretical preparation often leads to information overload and paralysis instead of real change. Relationships do not require more courses or advice but rather the courage to act and live in the present. Self-discovery alone is not enough—the key lies in self- acceptance and integrating knowledge into everyday life. The author highlights the trap of endless preparation, which prevents people from experiencing real relationships, emphasizing that relationship satisfaction stems from authenticity and the willingness to engage actively, rather than from pursuing perfection.
V dnešnej dobe je dôležité vedieť, čo naozaj jeme – najmä pri mäsových výrobkoch, kde sa čoraz častejšie objavujú prípady falšovania. Správna identifikácia a určenie množstva mäsa v produktoch pomáha odhaliť nepoctivé praktiky, chrániť zdravie spotrebiteľov a zároveň budovať dôveru voči výrobcom. Jednou z najspoľahlivejších metód, ako to dosiahnuť, je analýza proteínov. Najmä technika LC‐MS/MS (kvapalinová chromatografia spojená s hmotnostnou spektrometriou) sa ukazuje ako veľmi presný a citlivý nástroj, ktorý dokáže odhaliť aj malé množstvá mäsa, a to aj v zložitých alebo tepelne spracovaných potravinách. Vďaka tomu sa proteínové metódy stávajú čoraz dôležitejšou súčasťou potravinovej kontroly.
Knowing what we really eat is more important than ever – especially when it comes to meat products, where cases of fraud are still a concern. Being able to identify what kind of meat is present, and in what quantity, helps prevent dishonest practices, protects consumer health, and supports trust in the food industry. One of the most reliable ways to achieve this is through protein analysis. Techniques based on LC‐MS/MS (liquid chromatography coupled with mass spectrometry) stand out for their high accuracy and sensitivity. They can detect even small amounts of specific meat types, even in complex or processed foods. That’s why protein‐based methods are becoming a key part of modern food quality control and authenticity. testing.
Background: The COVID-19 pandemic disrupted traditional qualitative research methods, necessitating innovative approaches to data collection that avoided physical contact. Aim: To introduce and reflect upon audio diaries as a novel qualitative technique for capturing healthcare workers' lived experiences in crisis contexts. Methods: Healthcare professionals in Barcelona, Spain, recorded their thoughts, reflections, and concerns using WhatsApp audio notes. The study was conducted in two phases: the first during the initial pandemic wave (March-July 2020) and the second during a less restrictive period. Results: In the first phase, seven healthcare professionals provided 147 audio entries (2-10 minutes each). In the second phase, five professionals submitted 12 entries (26 seconds to 23 minutes). Audio diaries demonstrated several strengths, including efficiency, authenticity promotion, establishment of an informal therapeutic space, and the capturing of emotional content through voice tonality. Conclusion: Audio diaries offer a valuable alternative for collecting rich, transferable data in situations where traditional face-to-face techniques are not feasible, particularly in crisis contexts. This methodological innovation has implications for future qualitative research, especially in challenging or restricted environments.
JBI has long held the view that an inclusive approach to the conceptualization of what counts as evidence is important to the evidence-based movement. JBI's approach for appraising textual evidence had encompassed all forms of text (narrative, opinion, and policy), with one general tool used to guide critical appraisal. The proliferation of textual evidence and increase in textual evidence reviews demonstrate the need to reconceptualize JBI's methodological approach to critically appraising textual evidence. The objective of this paper is to outline the updated methodological approach to systematic reviews of textual evidence, especially in relation to the development of 3 separate critical appraisal tools for narrative, expert opinion, and policy text. Using an adapted Delphi approach, the JBI Textual Evidence Methodology Group convened over several rounds of meetings and discussions with international experts to reach consensus on the reconceptualization of critical appraisal tools for textual evidence sources. Strategies to effectively interrogate the legitimacy and authenticity of sources were found to be dependent upon the type of textual evidence under review. Therefore, 3 separate critical appraisal tools for narrative, expert opinion, and policy text were developed. This paper provides an overview of the development of 3 separate critical appraisal tools, highlighting the complex nature of textual evidence data sources.
BACKGROUND: The social space of birth-the birth environment, its occupants, and the human activities taking place-is interconnected with birth experiences. AIM: To investigate how the reality of the social space of birth affects women's positive birth experiences. METHODS: We combined open-text responses to the Babies Born Better survey from 3633 postpartum women in Austria, Belgium, the Czech Republic, Germany, Spain, the Netherlands, and the United Kingdom and 39 interview transcripts from Czech and Dutch postpartum women. We conducted a textual and thematic analysis. FINDINGS: Three themes and 11 categories were generated: (1) Exercising fundamental human agency in the birth space consists of the categories: 'exercising rights', 'the protection of human vulnerability', and 'the freedom to be authentic', which women regard as prerequisite components of the birth space. (2) Regulatory frameworks & care philosophies in maternity services, including the categories '(financial) regulations', 'values of the care provider and the institution', and 'model of care', are regarded as attributes of the birth space. Theme (3) Building a nest for comfort and connection comprises the categories 'relational and affective atmosphere during labour & birth', 'performative atmosphere during labour & birth', 'shelter', 'implicit and explicit tacit doing & being' and 'symbol of deeper meaning'. DISCUSSION/CONCLUSION: The reality of the birth space of women with positive birth experiences consists of human rights and birth rights, the quality of interactions with care providers during labour and birth in a relationship-centred and relation-continuity model of care, and a place to retreat from the world.
- MeSH
- Adult MeSH
- Qualitative Research MeSH
- Humans MeSH
- Mothers * psychology MeSH
- Postpartum Period psychology MeSH
- Parturition * psychology MeSH
- Surveys and Questionnaires MeSH
- Social Environment * MeSH
- Pregnancy MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Europe MeSH
Lipidomics and metabolomics communities comprise various informatics tools; however, software programs handling multimodal mass spectrometry (MS) data with structural annotations guided by the Lipidomics Standards Initiative are limited. Here, we provide MS-DIAL 5 for in-depth lipidome structural elucidation through electron-activated dissociation (EAD)-based tandem MS and determining their molecular localization through MS imaging (MSI) data using a species/tissue-specific lipidome database containing the predicted collision-cross section values. With the optimized EAD settings using 14 eV kinetic energy, the program correctly delineated lipid structures for 96.4% of authentic standards, among which 78.0% had the sn-, OH-, and/or C = C positions correctly assigned at concentrations exceeding 1 μM. We showcased our workflow by annotating the sn- and double-bond positions of eye-specific phosphatidylcholines containing very-long-chain polyunsaturated fatty acids (VLC-PUFAs), characterized as PC n-3-VLC-PUFA/FA. Using MSI data from the eye and n-3-VLC-PUFA-supplemented HeLa cells, we identified glycerol 3-phosphate acyltransferase as an enzyme candidate responsible for incorporating n-3 VLC-PUFAs into the sn1 position of phospholipids in mammalian cells, which was confirmed using EAD-MS/MS and recombinant proteins in a cell-free system. Therefore, the MS-DIAL 5 environment, combined with optimized MS data acquisition methods, facilitates a better understanding of lipid structures and their localization, offering insights into lipid biology.
- MeSH
- Data Mining * methods MeSH
- Phosphatidylcholines metabolism chemistry MeSH
- HeLa Cells MeSH
- Mass Spectrometry methods MeSH
- Humans MeSH
- Lipidomics * methods MeSH
- Lipids chemistry analysis MeSH
- Metabolomics methods MeSH
- Fatty Acids, Unsaturated metabolism chemistry MeSH
- Software MeSH
- Tandem Mass Spectrometry methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.
- MeSH
- Ceramides * blood MeSH
- Mass Spectrometry methods MeSH
- Calibration MeSH
- Laboratories * standards MeSH
- Humans MeSH
- Lipidomics methods MeSH
- Reference Standards * MeSH
- Reproducibility of Results MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
This article aims to describe the strategies that women suffering from dysmenorrhea (painful menstruation) use to cope with the pain and other physical and psychological difficulties accompanying this disease. Semi-structured interviews were conducted with eighteen women with dysmenorrhea and processed using thematic analysis. The results pointed to a significant connection between psychological and physical aspects and showed several passive and active strategies that women use. Pharmacotherapy in the form of analgesics and thermotherapy was used as first aid. What helped were rest and relaxation, reduction of external stimuli, and withdrawal into oneself, as well as emotional and physical catharsis and authentic pain relief, diversion of attention from pain, and social and emotional support of the immediate environment. Active strategies included various techniques such as yoga and breathing exercises, while passive strategies mainly included drug therapy and rest. Psychological support from family and friends also played a vital role. The study showed that a combination of these strategies can significantly contribute to better pain management and psychological well-being in women suffering from dysmenorrhea.
Autentizace rybích výrobků s využitím analýzy DNA vyžaduje zisk kvalitní DNA bez přítomnosti inhibitorů. V současné době jsou dostupné různé metody pro izolaci nukleových kyselin; pro svou rychlost a nenáročnost extrakčního postupu se staly velmi oblíbenými zejména silikátové centrifugační kolonky. Jejich nevýhodou však může být princip využívající záporný náboj DNA, který může být ovlivněn složením potravin, nebo jejich ucpání v důsledku špatné předúpravy vzorků. Cílem této práce bylo porovnat tři metody izolace DNA využívající různé principy (silikátové centrifugační kolonky, modifikované magnetické kuličky, cetyltrimethylamonium-bromid (CTAB) a chloroformová extrakce) a zhodnotit jejich vhodnost pro izolaci DNA z rybí svaloviny. Posuzovanými kritérii byla výtěžnost, čistota a amplifikovatelnost izolované DNA. Analyzována byla tkáň makrely obecné bez a s přídavkem přídatných látek běžně používaných při výrobě rybích produktů, konkrétně difosforečnanů (E 450) a barviv (E 110 a E 124), a následně byla vybraná metoda aplikována i na komerčně nabízené výrobky z ryb. Jako nejvhodnější se ukázala upravená metoda využívající detergent CTAB.
Authentication of fish products by DNA analysis requires the extraction of high quality DNA without the presence of inhibitors. Many nucleic acid isolation methods are currently available; silicate centrifugal columns have become very popular due to their speed and ease of extraction. However, their disadvantage may be the principle based on DNA charge, which may be affected by food composition, or clogging due to a poor sample pretreatment. The aim of this work was to compare three DNA isolation methods using different principles (silicate centrifugal columns, modified magnetic beads, Cetrimonium bromide and chloroform extraction) and to evaluate their suitability for DNA isolation from fish muscle. The criteria assessed were the recovery, purity and amplifiability of the isolated DNA. Mackerel tissue was analysed without and with the addition of additives commonly used in the manufacture of fish products, namely diphosphates (E 450) and colorants (E 110 and E 124), and the selected method was subsequently applied to commercial fish products. The modified method using the detergent CTAB proved to be the most suitable.
- MeSH
- Food Analysis methods MeSH
- DNA analysis isolation & purification MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Seafood analysis MeSH
- Food Additives analysis MeSH
- Fish Products * analysis MeSH
- Fishes MeSH
- Nucleic Acid Amplification Techniques methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
Autoři uvádějí přehled základních členění pornografického materiálu dle jeho obsahu a jeho znaky, které jej diferencují od erotiky. Kategorizace, například na mírnou, explicitní a násilnou, je důležitá pro účely sexuologických výzkumů, kde pornografie slouží jako podnětový materiál. Specifičtější členění je u pornografie dětské, což je přínosné pro následnou soudněznaleckou činnost při posuzování deliktu. Dominujícím médiem je v posledních letech internet. Pornografie se na něm rozvinula do nových alternativních forem, jako je netporno, altporno či etické porno. Jde o inovativní přístupy porno průmyslu nejen ve snaze o co nejrealističtější vyobrazení sexuálních praktik, ale i o poskytnutí rovnějších a příjemnějších podmínek zúčastněným aktérům. Vzrůst obliby, díky větší autentičnosti, oproti klasické pornografii, je i u pornografie amatérské, kterou si aktéři natáčejí sami a umísťují ji následně na internet. Problematické však může být zveřejnění obsahu bez konsensu všech zúčastněných. I to se bohužel děje a jde o jeden z dalších nových fenoménů v pornografii - revenge porno.
The authors give an overview of the basic breakdown of pornographic material according to its content and its features that differentiate it from erotica. The categorizations, such as mild, explicit, and violent, are important for the purposes of sexological research, where pornography serves as stimulus material. A more specific categorisation is for child pornography, which is useful for subsequent forensic work in assessing the offence. The dominant medium in recent years has been the Internet. Pornography has evolved into new alternative forms such as netporn, altporn and ethical porn. These are innovative approaches by the porn industry, not only in an attempt to portray sexual practices as realistically as possible, but also to provide more equal and pleasurable conditions for the actors involved. Amateur pornography, which is filmed by the actors themselves and posted on the Internet, is also growing in popularity due to its greater authenticity compared to ́classic pornography ́. However, publishing content without the consensus of all those involved can be problematic. Unfortunately, this is also happening and is one of the other new phenomena in pornography - revenge porn.
