microscopy techniques
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19 sv.
- MeSH
- elektronová mikroskopie MeSH
- mikrobiologické techniky MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- radiologie, nukleární medicína a zobrazovací metody
Melissopalynology is an important analytical method to identify botanical origin of honey. Pollen grain recognition is commonly performed by visual inspection by a trained person. An alternative method for visual inspection is automated pollen analysis based on the image analysis technique. Image analysis transfers visual information to mathematical descriptions. In this work, the suitability of three microscopic techniques for automatic analysis of pollen grains was studied. 2D and 3D morphological characteristics, textural and colour features, and extended depth of focus characteristics were used for the pollen discrimination. In this study, 7 botanical taxa and a total of 2482 pollen grains were evaluated. The highest correct classification rate of 93.05% was achieved using the phase contrast microscopy, followed by the dark field microscopy reaching 91.02%, and finally by the light field microscopy reaching 88.88%. The most significant discriminant characteristics were morphological (2D and 3D) and colour characteristics. Our results confirm the potential of using automatic pollen analysis to discriminate pollen taxa in honey. This work provides the basis for further research where the taxa dataset will be increased, and new descriptors will be studied.
- MeSH
- barva MeSH
- med analýza MeSH
- mikroskopie metody MeSH
- počítačové zpracování obrazu metody MeSH
- pyl klasifikace MeSH
- včelařství * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
12, 462 s. : il.
Microscopy handbooks ; 40
2nd ed. xiv, 170 s. : il.
- Klíčová slova
- Laboratorní technika,
- MeSH
- fluorescenční mikroskopie MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- chemie, klinická chemie
Practical approach series
1st ed. xxiv, 231 s.
- Klíčová slova
- imunofluorescence,
- MeSH
- fluorescence MeSH
- fluorescenční mikroskopie MeSH
- proteiny MeSH
- Publikační typ
- příručky MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- chemie, klinická chemie
- biochemie
The present study is bringing a comparison of surface morphology for various types of contact lenses. A novel method--scanning electron microscopy under aqueous conditions (cryo-SEM)--was tested for visualization of lenses at magnifications up to 2000x. For imaging lens surface on nanometre scale, we employed atomic force microscopy (AFM) in aqueous media. Various materials of lenses, based on silicone hydrogels or conventional hydrogels, were investigated. Total, 10 types of contact lenses from five manufacturers were selected and probed. We found that different methods of lens manufacture (lathe-cutting, cast-moulding, and spin casting) led to different values of surface roughness. In the swollen state, roughness values of lens surfaces lie between 4 and 140 nm. Lenses manufactured by lathe-cutting exhibit notable higher values, so that they could be easily distinguished from others. In cast-moulded lenses, the surface roughness decreased with increasing water content. Moreover, additional treatments of lenses introduced unique structural motifs onto surface. For instance, porous structure was found on lens surface finalized with plasma oxidation.
- MeSH
- biomedicínské technologie MeSH
- financování organizované MeSH
- hydrogely MeSH
- kontaktní čočky MeSH
- lidé MeSH
- mikroskopie atomárních sil metody MeSH
- mikroskopie elektronová rastrovací metody MeSH
- nanotechnologie MeSH
- povrchové vlastnosti MeSH
- roztoky MeSH
- silikony MeSH
- zmrazování MeSH
- Check Tag
- lidé MeSH
- MeSH
- elektronová mikroskopie MeSH
- fosfolipidy MeSH
- histologické techniky MeSH
- myši MeSH
- Check Tag
- myši MeSH
