Cíl studie: Ověření referenčního intervalu nové metody na stanovení volného trijodtyroninu v séru na analyzátoru Architect ci 16200 firmy Abbott. Typ studie: původní práce. Název a sídlo pracoviště: Institut klinické a experimentální medicíny, Vídeňská 1958/9, 140 21 Praha Materiál a metody: Referenční interval byl ověřen celkem na 110 vzorcích séra od zdravých jedinců, z nichž bylo 55 žen a 55 mužů. Všechny použité vzorky pocházely z Biobanky IKEM. Na stanovení byla použita souprava fT3_6, chemiluminiscenční imunoanalýza na mikročásticích firmy Abbott. Výsledky: Všechny naměřené hodnoty volného trijodtyroninu v séru novou metodou fT3_6 byly v referenčním intervalu, který udává výrobce. Referenční rozmezí, stanovené na vzorcích z Biobanky IKEM neparametrickou statistickou metodou s Box-Cox transformací bylo 3,21 - 4,78 pmol/l. Závěr: Referenční rozmezí fT3 stanovené novou metodou fT3_6 firmy Abbott, je možné v klinické praxi použít s rozmezím udávaným výrobcem.
Objective: Verification of the reference interval of a new method for the determination of free triiodothyronine in serum on the Abbott Architect 16200 analyzer. Design: Original Article. Settings: Institute for Clinical and Experimental Medicine, Vídeňská 1958/9, 140 21 Praha Material and methods: The reference interval was validated on a total of 110 serum samples from healthy subjects, comprising 55 females and 55 males. All samples used were from the IKEM Biobanka. The FT3_6 microparticle chemiluminescent immunoassay Abbott kit was used for the assay. Results: All free serum triiodothyronine values measured by the new fT3_6 method were in the reference interval indicated by the manufacturer. The reference range determined by a non-parametric statistical method with the Box-Cox transformation on the IKEM Biobanka samples was 3.21 - 4.78 pmol/L. Conclusion: The fT3 reference range established by the Abbott´s new fT3_6 method can be used in clinical practice with the range specified by the manufacturer.
- Keywords
- referenční interval,
- MeSH
- Adult MeSH
- Immunoassay methods MeSH
- Clinical Studies as Topic MeSH
- Middle Aged MeSH
- Humans MeSH
- Luminescent Measurements methods MeSH
- Reference Values * MeSH
- Serologic Tests methods MeSH
- Triiodothyronine * analysis blood MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- MeSH
- Urinalysis MeSH
- Humans MeSH
- Proteinuria MeSH
- Proteins analysis MeSH
- Reference Values MeSH
- Check Tag
- Humans MeSH
BACKGROUND: Free light chains, type kappa (FLC-K), in cerebrospinal fluid (CSF) were compared to oligoclonal IgG in many studies for sensitive detection of immune reactions in brain. The missing consensus about CSF data interpretation prevents reliable conclusions. This can be overcome by a theory-based hyperbolic reference range in CSF/serum quotient diagrams. METHODS: Mean Quotients for FLC-K, QKappa, and albumin, QAlb, of grouped, biochemically defined controls (N = 433) are fitted with the hyperbolic function QKappa(mean) = a/b (QAlb2 + b2)0.5 - c by a generally applicable procedure excluding outliers. RESULTS: With QKappa(mean), the coefficient of variation CV (22.5%) and the reference range (QKappa(mean) ± 3 CV) we got the discrimination line QKappa(lim) = (3.27(QAlb2 + 33)0.5-8.2) ×10-3 in a FLC-K Reibergram. Intrathecal FLC-K was found in 8% of another control group without OCB (N = 388) but was missed in 7% of patients with definite Multiple sclerosis (N = 95). In MS the mean intrathecal fraction was threefold larger for FLC-K (95%) compared to total IgG (36%). Similar mean quantities of intrathecal FLC-K contradict an immunological conversion between a Clinically isolated syndrome and MS. DISCUSSION: The hyperbolic reference range is superior to linear FLC-K Index (10 to 15% false negatives) and exponential curves (30% false positive interpretations for controls) in the analytical range of MS data, with excellent data fit for up to ten-fold larger QAlb values. Dynamics of the small molecule FLC-K contribute to the understanding of molecular size dependent barrier functions.
- MeSH
- Albumins analysis standards MeSH
- Immunoglobulin kappa-Chains analysis MeSH
- Humans MeSH
- Cerebrospinal Fluid chemistry MeSH
- Reference Values MeSH
- Retrospective Studies MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
3rd ed. 232 s.
- MeSH
- Biochemistry MeSH
- Diagnosis MeSH
- Child MeSH
- Electrolytes MeSH
- Humans MeSH
- Adolescent MeSH
- Reference Values MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Female MeSH
