software comparison
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- MeSH
- chemoterapie nádorů pomocí regionální perfúze MeSH
- diagnostické zobrazování metody MeSH
- dospělí MeSH
- funkce levé komory srdeční MeSH
- infarkt myokardu diagnóza patologie MeSH
- lidé MeSH
- nukleární lékařství metody MeSH
- počítačové zpracování obrazu MeSH
- radioisotopová ventrikulografie metody MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- srovnávací studie MeSH
BACKGROUND:Analysis of ciliary function for assessment of patients suspected of primary ciliary dyskinesia (PCD) and for research studies of respiratory and ependymal cilia requires assessment of both ciliary beat pattern and beat frequency. While direct measurement of beat frequency from high-speed video recordings is the most accurate and reproducible technique it is extremely time consuming. The aim of this study was to develop a freely available automated method of ciliary beat frequency analysis from digital video (AVI) files that runs on open-source software (ImageJ) coupled to Microsoft Excel, and to validate this by comparison to the direct measuring high-speed video recordings of respiratory and ependymal cilia. These models allowed comparison to cilia beating between 3 and 52 Hz. METHODS:Digital video files of motile ciliated ependymal (frequency range 34 to 52 Hz) and respiratory epithelial cells (frequency 3 to 18 Hz) were captured using a high-speed digital video recorder. To cover the range above between 18 and 37 Hz the frequency of ependymal cilia were slowed by the addition of the pneumococcal toxin pneumolysin. Measurements made directly by timing a given number of individual ciliary beat cycles were compared with those obtained using the automated ciliaFA system. RESULTS:The overall mean difference (± SD) between the ciliaFA and direct measurement high-speed digital imaging methods was -0.05 ± 1.25 Hz, the correlation coefficient was shown to be 0.991 and the Bland-Altman limits of agreement were from -1.99 to 1.49 Hz for respiratory and from -2.55 to 3.25 Hz for ependymal cilia. CONCLUSIONS:A plugin for ImageJ was developed that extracts pixel intensities and performs fast Fourier transformation (FFT) using Microsoft Excel. The ciliaFA software allowed automated, high throughput measurement of respiratory and ependymal ciliary beat frequency (range 3 to 52 Hz) and avoids operator error due to selection bias. We have included free access to the ciliaFA plugin and installation instructions in Additional file 1 accompanying this manuscript that other researchers may use.
Vzhledem k intermitentní a acyklické povaze sportovního výkonu hráčů sportovních her je nezbytné kombinovat analýzy vnějšího a vnitřního zatížení pro dobrou deskripci kondičních nároků utkání na hráče. Cílem studie bylo ověření reliability měření překonané vzdálenosti hráčů během utkání v házené pomocí systému Video Manual Motion Tracker 1.0 (VMMT 1.0). Videozáznam utkání pro následné posuzování překonané vzdálenosti hráček házené byl pořízen na utkání nejvyšší české soutěže dívek (n=14) v házené v kategorii U18. Čtyřicet zaškolených posuzovatelů vyhodnocovalo třikrát opakovaně překonanou vzdálenost hráček během utkání pro posouzení relativní a absolutní reliability a 2 posuzovatelé zaznamenávali překonanou vzdálenost padesáti odlišných úseků herního děje sledovaných hráček pro posouzení objektivity. Pro výpočet systematické chyby jsme použili jednofaktorovou analýzu rozptylu, dále vnitrotřídní koeficient korelace pro hodnocení intra-subjektové reliability, Pearsonův koeficient korelace pro posouzení objektivity měření a hodnoty absolutní reliability (standardní chyba měření a Bland-Altmanovy 95% limity shody). Studie prokázala srovnatelně vysokou reliabilitu měření s ostatními využívanými systémy
Due to intermittent character of performance in games it is important to combine more methods to describe the load of the players. Former research showed the combination of time-motion analysis and heart rate is appropriate. The goal of this study was to evaluate the reliability of the distance covered measurement by Video Manual Motion Tracker 1.0 during handball match. One warm up handball match (14 players) was recorded to time-motion analysis. Forty instructed observers rated the distance covered repeatedly (three times) to relative and absolute reliability assessment. Then two instructed observers rated the distance covered of fifty match sequences to objectivity assessments. One-way ANOVA was used to detect systematic bias. ICC was calculated to determine intra-rater reliability whereas a coefficient of Pearson product moment correlation was used to express objectivity. The magnitude of absolute reliability was expressed by standard error of measurement (SEM) and by Bland and Altman’s 95% limits of agreement of the two repeated measures. The results of the study showed high reliability of measurement in comparison with other systems.
- Klíčová slova
- analýza herního výkonu, zatížení, utkání, házená,
- MeSH
- lidé MeSH
- mladiství MeSH
- software * MeSH
- sporty fyziologie MeSH
- výkonnost * klasifikace MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- ženské pohlaví MeSH
The linear theory of electromigration, including the first-order nonlinear approximation, is generalized to systems with any equilibria fast enough to be considered instantaneous in comparison with the timescale of peak movement. For example, this theory is practically applied in the electrokinetic chromatography (EKC) mode of the CZE. The model enables the calculation of positions and shapes of analyte and system peaks without restricting the number of selectors, the complexation stoichiometry, or simultaneous acid-base equilibria. The latest version of our PeakMaster software, PeakMaster 6-Next Generation, implements the theory in a user-friendly way. It is a free and open-source software that performs all calculations and shows the properties of the background electrolyte and the expected electropherogram within a few seconds. In this paper, we mathematically derive the model, discuss its applicability to EKC systems, and introduce the PeakMaster 6 software.
PURPOSE: Neuroimaging pipelines have long been known to generate mildly differing results depending on various factors, including software version. While considered generally acceptable and within the margin of reasonable error, little is known about their effect in common research scenarios such as inter-group comparisons between healthy controls and various pathological conditions. The aim of the presented study was to explore the differences in the inferences and statistical significances in a model situation comparing volumetric parameters between healthy controls and type 1 diabetes patients using various FreeSurfer versions. METHODS: T1- and T2-weighted structural scans of healthy controls and type 1 diabetes patients were processed with FreeSurfer 5.3, FreeSurfer 5.3 HCP, FreeSurfer 6.0 and FreeSurfer 7.1, followed by inter-group statistical comparison using outputs of individual FreeSurfer versions. RESULTS: Worryingly, FreeSurfer 5.3 detected both cortical and subcortical volume differences out of the preselected regions of interest, but newer versions such as FreeSurfer 5.3 HCP and FreeSurfer 6.0 reported only subcortical differences of lower magnitude and FreeSurfer 7.1 failed to find any statistically significant inter-group differences. CONCLUSION: Since group averages of individual FreeSurfer versions closely matched, in keeping with previous literature, the main origin of this disparity seemed to lie in substantially higher within-group variability in the model pathological condition. Ergo, until validation in common research scenarios as case-control comparison studies is included into the development process of new software suites, confirmatory analyses utilising a similar software based on analogous, but not fully equivalent principles, might be considered as supplement to careful quality control.
The recent discoveries of regulatory non-coding RNAs changed our view of RNA as a simple information transfer molecule. Understanding the architecture and function of active RNA molecules requires methods for comparing and analyzing their 3D structures. While structural alignment of short RNAs is achievable in a reasonable amount of time, large structures represent much bigger challenge. Here, we present the SETTER web server for the RNA structure pairwise comparison utilizing the SETTER (SEcondary sTructure-based TERtiary Structure Similarity Algorithm) algorithm. The SETTER method divides an RNA structure into the set of non-overlapping structural elements called generalized secondary structure units (GSSUs). The SETTER algorithm scales as O(n(2)) with the size of a GSSUs and as O(n) with the number of GSSUs in the structure. This scaling gives SETTER its high speed as the average size of the GSSU remains constant irrespective of the size of the structure. However, the favorable speed of the algorithm does not compromise its accuracy. The SETTER web server together with the stand-alone implementation of the SETTER algorithm are freely accessible at http://siret.cz/setter.
- MeSH
- algoritmy MeSH
- internet MeSH
- konformace nukleové kyseliny MeSH
- RNA chemie MeSH
- software MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Benchmarking and monitoring of urban design and transport features is crucial to achieving local and international health and sustainability goals. However, most urban indicator frameworks use coarse spatial scales that either only allow between-city comparisons, or require expensive, technical, local spatial analyses for within-city comparisons. This study developed a reusable, open-source urban indicator computational framework using open data to enable consistent local and global comparative analyses. We show this framework by calculating spatial indicators-for 25 diverse cities in 19 countries-of urban design and transport features that support health and sustainability. We link these indicators to cities' policy contexts, and identify populations living above and below critical thresholds for physical activity through walking. Efforts to broaden participation in crowdsourcing data and to calculate globally consistent indicators are essential for planning evidence-informed urban interventions, monitoring policy effects, and learning lessons from peer cities to achieve health, equity, and sustainability goals.
- MeSH
- celosvětové zdraví * MeSH
- lidé MeSH
- prostorová analýza MeSH
- software MeSH
- velkoměsta MeSH
- zdravotní stav * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Research Support, N.I.H., Extramural MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Geografické názvy
- velkoměsta MeSH
