triplex
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Kontinuálny únik moču pri súčasne normálnej mikcii môže byť príznakom ektopického vyústenia močovodu u dievčat. Prezentujem kazuistiku 6 roč- ného dievčatka, ktoré trpelo nepretržitým denným aj nočným únikom moču. Diagnostika a terapia u die- ťaťa bola uskutočnená na Klinike pediatrickej urológie. V diagnostike sme použili sonografiu, vylučovaciu urografiu, cystoskopiu, vaginoskopiu a retrográdnu ureteropyelografiu. Vyšetreniami sme zistili raritne sa vyskytujúci triplexný močovod (II. typu podľa Smithovej klasifikácie) vľavo s ureter fissus dolného segmentu a naviac so sub-sfinkterickým ektopickým vyústením močovodu horného segmentu do uretry. Podozrenie na prítomnosť tretieho močovodu vzniklo potom, keď sme upresnili anatómiu ureteru dolného segmentu v zmysle ureter fissus pri klinicky jasnej ureterickej inkontinencii, aj prítomnej funkcii horného segmentu pri vylučovacej urografii. Ako terapeutický postup sme zvolili hemiureteronefrektómiu. Po výkone u dievčaťa prestal únik moču.
Continuous leakage of urine despite simultaneous normal micturition can be a sign of an ectopic ureter in girls. The case of 6 year old girl who suffered from continuous daylight and night time urine leakage is reported. Diagnosis and treatment of the child was done at the Clinic of paediatric urology. In diagnostics, we used sonography, excretory urography, cystoscopy, vaginoscopy and retrograde ureteropyelography. We found a rarely occurring triplex ureter on the left side and a sub-sphincteric ectopic ureter – with outlet into urethra. It was a case of ureteric incontinence. As a therapeutic approach, we chose hemiureteronephrectomy. In girl stopped urine leakage after surgery
- Klíčová slova
- ektopický močovod, triplexní močovod, ureterická inkontinence,
- MeSH
- dítě MeSH
- inkontinence moči etiologie chirurgie MeSH
- lidé MeSH
- nemoci močovodu * diagnóza chirurgie MeSH
- ureter abnormality chirurgie MeSH
- urologické chirurgické výkony metody MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
51 l. : grafy ; 32 cm
Projekt je zaměřen na studium genově cílených triplax tvořících oligonukleotidů a jejich konjugátů s DNA-destrujícími molekulami aktivovatelnými blízce infračerveným zářením v genové terapii HIV infekce. Projekt je založen na vyhledávání vhodných sekvencí pro TFO v genomu proviru HIV,návrh TFO a studium vazebných a štěpících schopnosti TFO in vitro a v buněčné kultuře metodami blíže popsanými v tomto projektu.; Project is focused on study of gene tergeted triplex forming oligonucleotides (TFO) and their conjugates with red-shifted wavelenghts photoactivable DNA-destructing molecules in anti-HIV gene therapy. The project is based on triplex terget sites in HIV provirus search,TFOs designing and evalution of binding and cleavage properties of TFOs in vitro and in cell culture by in project described methods.
MOTIVATION: Upgrade and integration of triplex software into the R/Bioconductor framework. RESULTS: We combined a previously published implementation of a triplex DNA search algorithm with visualization to create a versatile R/Bioconductor package 'triplex'. The new package provides functions that can be used to search Bioconductor genomes and other DNA sequence data for occurrence of nucleotide patterns capable of forming intramolecular triplexes (H-DNA). Functions producing 2D and 3D diagrams of the identified triplexes allow instant visualization of the search results. Leveraging the power of Biostrings and GRanges classes, the results get fully integrated into the existing Bioconductor framework, allowing their passage to other Genome visualization and annotation packages, such as GenomeGraphs, rtracklayer or Gviz. AVAILABILITY: R package 'triplex' is available from Bioconductor (bioconductor.org). CONTACT: lexa@fi.muni.cz SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
MOTIVATION: Current methods for identification of potential triplex-forming sequences in genomes and similar sequence sets rely primarily on detecting homopurine and homopyrimidine tracts. Procedures capable of detecting sequences supporting imperfect, but structurally feasible intramolecular triplex structures are needed for better sequence analysis. RESULTS: We modified an algorithm for detection of approximate palindromes, so as to account for the special nature of triplex DNA structures. From available literature, we conclude that approximate triplexes tolerate two classes of errors. One, analogical to mismatches in duplex DNA, involves nucleotides in triplets that do not readily form Hoogsteen bonds. The other class involves geometrically incompatible neighboring triplets hindering proper alignment of strands for optimal hydrogen bonding and stacking. We tested the statistical properties of the algorithm, as well as its correctness when confronted with known triplex sequences. The proposed algorithm satisfactorily detects sequences with intramolecular triplex-forming potential. Its complexity is directly comparable to palindrome searching. AVAILABILITY: Our implementation of the algorithm is available at http://www.fi.muni.cz/lexa/triplex as source code and a web-based search tool. The source code compiles into a library providing searching capability to other programs, as well as into a stand-alone command-line application based on this library. CONTACT: lexa@fi.muni.cz SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
- MeSH
- algoritmy MeSH
- chybné párování bází MeSH
- DNA chemie metabolismus MeSH
- Escherichia coli K12 genetika MeSH
- genom MeSH
- konformace nukleové kyseliny MeSH
- lidé MeSH
- obrácené repetice MeSH
- pravděpodobnostní funkce MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
"Mycobacterium avium subsp. hominissuis" often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment. We confirmed the diagnoses by detecting M. avium subsp. hominissuis using qPCR with DNA directly isolated from lymph node biopsy specimens of two patients. In order to trace the source of infection from the environment, a method of DNA isolation from soil and other environmental samples, such as dust, cobwebs, and compost, was developed. The triplex qPCR examination revealed the presence of M. avium subsp. hominissuis in a high proportion of the environmental samples (42.8% in the first patient's house and 47.6% in the second patient's house). Both patients were also exposed to M. avium subsp. avium, which was present due to the breeding of infected domestic hens. The high infectious dose of M. avium subsp. hominissuis or the increased susceptibility of humans to M. avium subsp. hominissuis compared to M. avium subsp. avium could be the reason why the children were infected with M. avium subsp. hominissuis.
- MeSH
- bakteriologické techniky metody MeSH
- dítě MeSH
- krk mikrobiologie MeSH
- lidé MeSH
- mikrobiologie životního prostředí MeSH
- molekulární typizace MeSH
- Mycobacterium avium klasifikace genetika růst a vývoj izolace a purifikace MeSH
- polymerázová řetězová reakce metody MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- předškolní dítě MeSH
- tuberkulóza lymfatických uzlin diagnóza mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- práce podpořená grantem MeSH
The aim of this study was to optimise a two-tube reverse transcription triplex quantitative real time PCR (qRT-PCR) combining amplification of two loci with an internal amplification control (IAC) for detection and quantitation of hepatitis E virus (HEV) RNA and to validate its performance on a pool of biological samples. Optimisation was performed on serially diluted "home-made" RNA standards. The limit of detection was determined experimentally as 10 copies/μl of the RNA standard for both amplification targets. The qRT-PCR was validated on a cohort of samples originating from 48 wild boars (Sus scrofa), 17 fallow deer (Dama dama) and 28 mouflons (Ovis musimon), with nested RT-PCR used as a reference method. qRT-PCR was found to be more specific for the detection of HEV RNA in examined samples. HEV RNA was detected in samples of five more animals (one wild boar and four mouflons) in comparison with nested RT-PCR.
- MeSH
- hepatitida E diagnóza veterinární virologie MeSH
- ovce virologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí metody normy MeSH
- reprodukovatelnost výsledků MeSH
- RNA virová analýza genetika normy MeSH
- senzitivita a specificita MeSH
- Sus scrofa virologie MeSH
- virus hepatitidy E genetika MeSH
- vysoká zvěř virologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
