- Klíčová slova
- DebriEcaSan, HyalEcaSan,
- MeSH
- dehiscence operační rány etnologie MeSH
- dospělí MeSH
- hojení ran * účinky léků MeSH
- kyselina hyaluronová analogy a deriváty farmakologie terapeutické užití MeSH
- lidé MeSH
- mamoplastika škodlivé účinky MeSH
- pooperační komplikace farmakoterapie MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- amnion MeSH
- amputace MeSH
- biologické krytí * ekonomika klasifikace MeSH
- diabetická noha * komplikace ošetřování patologie terapie MeSH
- estery aplikace a dávkování MeSH
- hojení ran MeSH
- kyselina hyaluronová analogy a deriváty aplikace a dávkování MeSH
- lidé MeSH
- randomizované kontrolované studie jako téma MeSH
- vředy na noze (od hlezna dolů) etiologie ošetřování terapie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
We present water-insoluble hyaluronan films crosslinked by trivalent iron developed as potential resorbable implants. The films were crosslinked by sorption of ferric salt into solid HA films in water/2-propanol bath. These heterogeneously crosslinked films (het-FeHA) remained tough and dimensionally stable when rehydrated in saline. In contrast, films prepared by drying the well-known homogeneous ferric hyaluronate gels (hom-FeHA) softened upon rehydration and expanded rapidly. Differences between hom-FeHA and het-FeHA result from polymer network topology (dominant inter- or intra-molecular crosslink, respectively). Moreover, Mössbauer spectroscopy of het-FeHA revealed diiron complexes, while iron in the hom-FeHA was present exclusively as γ-FeOOH nanoparticles or amorphous FeOOH. The biocompatibility tests of het-FeHA did not show any adverse effect and the sample disintegrated within one day when implanted in mice peritoneum. In conclusion, we developed implantable hyaluronan-based free-standing film with minimal swelling that can be resorbed quickly enough to avoid induction of foreign-body reaction.
- MeSH
- buňky 3T3 MeSH
- hydrogely chemie MeSH
- kyselina hyaluronová analogy a deriváty MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- nanočástice chemie MeSH
- peritoneum chirurgie MeSH
- reagencia zkříženě vázaná chemie MeSH
- vstřebatelné implantáty * MeSH
- železo chemie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Ophthalmic disorders are amid the most common pathologies worldwide. In this regard, hyaluronan (HA) has gained widespread use in eye surgeries and treatment of eye diseases. HA-based materials are well tolerated and show excellent biocompatibility. Therefore, HA is a sought-after component of ophthalmic formulations. However, several parameters have to be adjusted to reach an optimal performance and effectiveness. The complexity of HA properties represents a major challenge for biological and structural characterization. This review covers fundamental aspects and recent developments in HA ophthalmic research. Biological mechanisms underlying the health benefits of HA and their potential therapeutic applications are elaborated. Furthermore, current pharmacokinetic and safety studies are discussed. Noticeably, the benefits of chemical modification and processed HA forms are described to broaden the applications of this unique polysaccharide. The current research challenges and prospects are also outlined and discussed.
- MeSH
- antitumorózní látky chemie metabolismus MeSH
- glaukom farmakoterapie MeSH
- kyselina hyaluronová analogy a deriváty terapeutické užití MeSH
- lidé MeSH
- nosiče léků chemie MeSH
- oční nemoci farmakoterapie MeSH
- oční roztoky chemie MeSH
- viskozita MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Klíčová slova
- Dona, Condrosulf, Hyalgan, Synvisc, Synovial, Artrodal, Piascledine 300,
- MeSH
- chondroitin sulfáty farmakologie terapeutické užití MeSH
- glukosamin aplikace a dávkování farmakologie MeSH
- kyselina hyaluronová analogy a deriváty terapeutické užití MeSH
- lidé MeSH
- osteoartróza * chirurgie farmakoterapie MeSH
- paracetamol aplikace a dávkování farmakologie MeSH
- potravní doplňky MeSH
- proteiny ze sójových bobů terapeutické užití MeSH
- rostlinné extrakty terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Klíčová slova
- Sorelex,
- MeSH
- antiinfekční látky lokální MeSH
- debridement MeSH
- dospělí MeSH
- komplikace těhotenství MeSH
- kožní vředy ošetřování terapie MeSH
- kyselina hyaluronová analogy a deriváty terapeutické užití MeSH
- lidé MeSH
- obvazy hydrokoloidní MeSH
- předloktí MeSH
- pyoderma gangrenosum * diagnóza ošetřování terapie MeSH
- pyridiny terapeutické užití MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation. Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles. These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture. Brno culture bioengineered with a fibrin-based scaffold contains round cells showing features of differentiated chondrocytes expressing S-100 protein and α-smooth muscle actin. In contrast, in the case of Hyalograft C, the scaffold was made up of a fibrillar network composed of biomaterial fibres of the esters of hyaluronic acid and cells resembling fibroblasts and myofibroblasts and expressing only α-smooth muscle actin. The average size of the defects was 2.5 cm2. Patients were evaluated using the standardized guidelines of the International Knee Documentation Committee. During the comparison of bioptic samples obtained from both patient cohorts, we did not observe any important differences in the histological makeup of the newly formed cartilage. The histological analysis of these two groups of homogeneous patients shows that this bioengineered approach, under proper indications, may offer favourable and stable clinical results over time, in spite of the different matrix and cellular composition of the two transplants used.
- MeSH
- aktiny metabolismus MeSH
- artroskopie MeSH
- biokompatibilní materiály chemie MeSH
- biopsie MeSH
- buněčná diferenciace MeSH
- chondrocyty cytologie transplantace MeSH
- chrupavka patologie MeSH
- dospělí MeSH
- femur patologie MeSH
- fibroblasty cytologie MeSH
- kohortové studie MeSH
- koleno patologie MeSH
- kyselina hyaluronová analogy a deriváty chemie MeSH
- lidé MeSH
- proteiny S100 metabolismus MeSH
- tkáňové inženýrství metody MeSH
- transplantace buněk metody MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
Native hyaluronan (HA) has been oxidized to polyaldehyde polymers with a degree of substitution (DS) of up to 50%. Two different procedures enabling the control of the degree of substitution were followed in this study. Selective oxidation of primary hydroxyl groups of N-acetyl-D-glucosamine of hyaluronan was performed either in an aqueous solution containing AcNH-TEMPO/NaBr/NaOCl or in an aprotic solvent containing Dess-Martin periodinane (DMP). It was found that a change of reaction parameters (reaction time and temperature, type of catalyst, oxidant-to-HA ratio, presence of nitrogen, buffer type, and concentration) had an influence on the degree of substitution and molecular weight. The derivatives were characterized by MS, NMR spectroscopy, and SEC-MALLS. Degradation of hyaluronic acid by the oxidant was observed and confirmed by SEC. The effect of oxidized derivatives of hyaluronan on cells was studied by means of NIH 3T3 fibroblast viability, which indicates that prepared hyaluronan polyaldehydes are biocompatible and suitable for medical applications and tissue engineering. The function of polyaldehyde as precursor for other modification was illustrated in the reaction with lysine.
- MeSH
- acetylglukosamin chemie MeSH
- aldehydy chemická syntéza farmakologie MeSH
- biokompatibilní materiály chemická syntéza farmakologie MeSH
- biopolymery chemie farmakologie MeSH
- bromidy chemie MeSH
- buňky NIH 3T3 MeSH
- chlornan sodný chemie MeSH
- cyklické N-oxidy chemie MeSH
- dusík chemie MeSH
- iminopyranosy chemie MeSH
- katalýza MeSH
- kyselina hyaluronová analogy a deriváty chemická syntéza farmakologie MeSH
- magnetická rezonanční spektroskopie MeSH
- molekulová hmotnost MeSH
- myši MeSH
- oxidace-redukce MeSH
- sloučeniny sodíku chemie MeSH
- teplota MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
