• MeSH
• hormony štítné žlázy krev   MeSH
• kolostrum MeSH
• novorozená zvířata fyziologie   MeSH
• skot MeSH
• Check Tag
• skot MeSH

• MeSH
• aminokyseliny izolace a purifikace   MeSH
• fyziologie výživy kojenců MeSH
• hormon uvolňující gonadotropiny izolace a purifikace   MeSH
• kojení MeSH
• kolostrum analýza   MeSH
• mateřské mléko analýza   MeSH

• MeSH
• alergeny MeSH
• imunita MeSH
• imunoglobuliny MeSH
• kolostrum imunologie   MeSH
• mateřské mléko imunologie   MeSH

• MeSH
• antiinfekční látky MeSH
• dítě MeSH
• imunoglobuliny MeSH
• kojenec MeSH
• kolostrum analýza   imunologie   MeSH
• lidé MeSH
• muramidasa MeSH
• novorozenec MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH

• MeSH
• alergie farmakoterapie   MeSH
• dítě MeSH
• imunitní systém MeSH
• imunizace MeSH
• imunoglobuliny MeSH
• kolostrum imunologie   MeSH
• lidé MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH

• Klíčová slova
• multiImun,
• MeSH
• kolostrum imunologie   MeSH
• lidé MeSH
• odolnost vůči nemocem imunologie   účinky léků   MeSH
• přirozená imunita účinky léků   MeSH
• Check Tag
• lidé MeSH

• MeSH
• buněčná imunita MeSH
• epitopy imunologie   MeSH
• kur domácí imunologie   MeSH
• nemoci zvířat prevence a kontrola   MeSH
• skot MeSH
• techniky in vitro MeSH
• transfer faktor MeSH
• viry imunologie   MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH

• MeSH
• antilymfocytární sérum imunologie   MeSH
• kolostrum imunologie   MeSH
• skot MeSH
• Check Tag
• skot MeSH

BACKGROUND: Human colostrum and milk contain components that influence development. Our aim was to use a protein array to determine the cytokine profile of human lacteal secretions and changes that occur during the early postpartum period. METHODS: We collected 17 samples of colostrum during the first 2 days postpartum and a 2nd group of 5 sets of 2 to 3 sequential colostrum or milk samples (at 20- to 30-h intervals). We analyzed the samples with array membranes consisting of 42 or 79 antibodies directed against cytokines. RESULTS: In most samples, we detected the previously described cytokines interleukin-8 (IL-8)/CXCL8, epidermal growth factor (EGF), growth-related oncoprotein (GRO)/CXCL1-3, angiogenin, transforming growth factor beta-2, and monocyte chemotactic protein 1 (MCP-1/CCL2). In addition, we found 32 cytokines that have not been described before in colostrum. Cytokine concentrations differed among mothers, and the spectrum of cytokines changed with time after delivery. A significant decrease occurred in IL-12 and macrophage inflammatory protein-1delta/CCL15 and a significant increase in MCP-1/CCL2. The production of angiogenin, vascular endothelial growth factor, GRO/CXCL1-3, EGF, and IL-8/CXCL8 remained high throughout. The concentrations of 2 selected cytokines measured with the array technique and ELISA showed moderate to strong correlation (r = 0.63 for EGF and r = 0.84 for IL-8/CXCL8). CONCLUSION: Despite the lack of precise quantification, the protein array might be suitable for cytokine screening. It allows simultaneous detection of a broad spectrum of cytokines (including those not described before) in lacteal secretions.

• MeSH
• časové faktory MeSH
• chemokiny analýza   MeSH
• čipová analýza proteinů MeSH
• cytokiny analýza   MeSH
• dospělí MeSH
• ELISA MeSH
• financování organizované MeSH
• kolostrum chemie   MeSH
• lidé MeSH
• mateřské mléko chemie   MeSH
• mezibuněčné signální peptidy a proteiny analýza   MeSH
• poporodní období MeSH
• proteomika MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• srovnávací studie MeSH

The first aim of our study was to obtain information on the transmission of antigen-specific antibodies from colostrum to respiratory tract mucosa in piglets. The second aim was to confirm the biological relevance of the presence of lymphocytes in colostrum and the already described fact that these cells can penetrate the intestinal barrier and "colonize" peripheral blood and lymphatic tissues of piglets. Therefore, we performed an experiment in which sows were immunized with a model antigen Keyhole Limpet Hemocyanin and their piglets were euthanized at different intervals after birth and colostrum intake. Colostrum, bronchoalveolar lavage fluid and blood samples were collected for serological detection of antigen-specific antibodies. Lymphocytes isolated from peripheral blood and lymphatic tissues (mesenteric and tracheobronchial lymph nodes and spleen) of piglets were in vitro activated with the antigen. We found that colostrum-derived antibodies can cross into the respiratory tract mucosa. Furthermore, we found that antigen-specific lymphocytes were detectable in mesenteric lymph nodes and peripheral blood, but very rarely in spleen and tracheobronchial lymph nodes.

• MeSH
• aktivace lymfocytů imunologie   MeSH
• buněčná imunita imunologie   MeSH
• humorální imunita imunologie   MeSH
• imunita získaná od matky imunologie   MeSH
• kolostrum imunologie   MeSH
• lymfoidní tkáň imunologie   MeSH
• novorozená zvířata imunologie   MeSH
• prasata imunologie   MeSH
• respirační sliznice imunologie   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH