Mikrobiom střeva hraje klíčovou roli v dlouhodobém fyzickém i psychickém zdraví člověka. Jeho vhodné složení během počáteční kolonizace gastrointestinálního traktu novorozenců s dostatečným zastoupením taxonů s komenzálním či probiotickým potenciálem je zásadní pro obranu před infekcemi a správný vývoj imunitního systému. Enterobakterie tvoří nedílnou součást střevní mikrobioty a mají klíčovou úlohu v počáteční kolonizaci střeva novorozence. Zároveň se jedná o potenciální patogeny, které mohou způsobovat závažné infekce. V článku jsou popsány funkce enterobakterií v mikrobiotě kojenců, rizika spojená s jejich nadměrnou přítomností a strategie prevence infekcí. Dále jsou diskutovány faktory ovlivňující formování mikrobioty u dětí, včetně způsobu porodu a vlivu antibiotik. Výzkumy ukazují, že podpora přirozeného porodu, kojení a použití probiotik mohou pozitivně ovlivnit střevní mikrobiotu a eliminovat potenciální rizika spojená s enterobakteriemi. Článek poskytuje přehled současných poznatků o enterobakteriích v mikrobiotě kojenců a zdůrazňuje potřebu dalšího výzkumu a sdílení nových poznatků v klinické praxi, aby byl zajištěn zdravý vývoj dětí.

The gut microbiome plays a key role in a person's long-term physical and psychological health. Its appropriate composition during the initial colonization of the gastrointestinal tract of newborns with the sufficient representation of the taxa with commensal or probiotic potential is essential for defence against infections and proper development of the immune system. Enterobacteria form an integral part of the intestinal microbiota and play a vital role in the initial colonization of the newborn gut. At the same time, these are potential pathogens that can cause serious infections. This article describes the functions of enterobacteria in the microbiota of infants, the risks associated with their excessive presence, and strategies for preventing infections. Furthermore, factors affecting microbiota formation in children are discussed, including the delivery method and the effect of antibiotics. Research shows that promoting natural childbirth, breastfeeding, and probiotic use can positively influence the gut microbiota and eliminate potential risks associated with enterobacteria. The article provides an overview of current knowledge about enterobacteria in the microbiota of infants and highlights the need for further research and the sharing of new knowledge in clinical practice to ensure the healthy development of children.

• MeSH
• Enterobacteriaceae * imunologie   MeSH
• enterovirové infekce imunologie   mikrobiologie   MeSH
• hygiena MeSH
• kojení MeSH
• lidé MeSH
• mikrobiota * imunologie   MeSH
• nemoci novorozenců imunologie   metabolismus   MeSH
• novorozenec MeSH
• přirozený porod MeSH
• probiotika MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH

The ReAct (Recovery, Activity) project is an ENFSI (European Network of Forensic Science Institutes) supported initiative comprising a large consortium of laboratories. Here, the results from more than 23 laboratories are presented. The primary purpose was to design experiments simulating typical casework circumstances; collect data and to implement Bayesian networks to assess the value (i.e., likelihood ratio) of DNA results given activity level propositions. Two different experimental designs were used to simulate a robbery, where a screwdriver was used to force a door or window. Propositions and case information were chosen following laboratory feedback listing typical casework circumstances (included in the paper). In a direct transfer experiment, the defendant owned and used the screwdriver, but he did not force the door/window in question. An unknown person used the defendant's stolen screwdriver. In an indirect transfer experiment, the defendant neither owned, saw, nor used the screwdriver, nor did they force the door or window. For the second experiment, given the defence view, the defendant never held the screwdriver. We envisaged the situation where an object manipulated by the defendant (or the defendant himself/herself) would be touched by the unknown offender who would then force the window. It was found for the direct transfer experiment that unless a single contributor profile aligning with the known person's of interest profile was retrieved, the results did not allow to discriminate between propositions. On the other hand, for the indirect transfer experiment, both single and major contributor profiles that aligned with the person of interest (POI) supported the proposition that the person used the tool rather than an unknown person who had touched an object, when indeed the former was true. There was considerable variation in median recoveries of DNA between laboratories (between 200pg-5ng) for a given experiment if quantities are taken into account. These differences affect the likelihood ratios given activity level propositions. More than 2700 samples were analysed in the course of this study. Two different Bayesian Networks are made available via an open source application written in Shiny R: Shiny_React(). For comparison, all datasets were analysed using a qualitative method categorised into absent, single, major or other given contributors. The importance of standardising methods is emphasised, alongside the necessity of developing new approaches to assign the probability of laboratory-dependent DNA recovery. Freely accessible open databases play a crucial role in supporting these efforts.

• MeSH
• Bayesova věta * MeSH
• DNA fingerprinting * MeSH
• DNA * genetika   MeSH
• laboratoře * MeSH
• lidé MeSH
• mikrosatelitní repetice MeSH
• pravděpodobnostní funkce MeSH
• soudní genetika metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Species belonging to the Mycobacterium kansasii complex (MKC) are frequently isolated from humans and the environment and can cause serious diseases. The most common MKC infections are caused by the species M. kansasii (sensu stricto), leading to tuberculosis-like disease. However, a broad spectrum of virulence, antimicrobial resistance and pathogenicity of these non-tuberculous mycobacteria (NTM) are observed across the MKC. Many genomic aspects of the MKC that relate to these broad phenotypes are not well elucidated. Here, we performed genomic analyses from a collection of 665 MKC strains, isolated from environmental, animal and human sources. We inferred the MKC pangenome, mobilome, resistome, virulome and defence systems and show that the MKC species harbours unique and shared genomic signatures. High frequency of presence of prophages and different types of defence systems were observed. We found that the M. kansasii species splits into four lineages, of which three are lowly represented and mainly in Brazil, while one lineage is dominant and globally spread. Moreover, we show that four sub-lineages of this most distributed M. kansasii lineage emerged during the twentieth century. Further analysis of the M. kansasii genomes revealed almost 300 regions of difference contributing to genomic diversity, as well as fixed mutations that may explain the M. kansasii's increased virulence and drug resistance.

• MeSH
• atypické mykobakteriální infekce * mikrobiologie   MeSH
• fylogeneze * MeSH
• genom bakteriální * MeSH
• genomika * MeSH
• lidé MeSH
• Mycobacterium kansasii * genetika   klasifikace   izolace a purifikace   MeSH
• virulence genetika   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Viral infection may represent a stress condition to the host cell. Cells react to it by triggering the defence programme to restore homeostasis and these events may in turn impact the viral replication. The knowledge about tick-borne encephalitis virus (TBEV) infection-associated stress is limited. Here we investigated the interplay between TBEV infection and stress pathways in PMJ2-R mouse macrophage cell line, as macrophages are the target cells in early phases of TBEV infection. First, to determine how stress influences TBEV replication, the effect of stress inducers H2O2 and tunicamycin (TM) was tested. Viral multiplication was decreased in the presence of both stress inducers suggesting that the stress and cellular stress responses restrict the virus replication. Second, we investigated the induction of oxidative stress and endoplasmic reticulum (ER) stress upon TBEV infection. The level of oxidative stress was interrogated by measuring the reactive oxygen species (ROS). ROS were intermittently increased in infected cells at 12 hpi and at 72 hpi. As mitochondrial dysfunction may result in increased ROS level, we evaluated the mitochondrial homeostasis by measuring the mitochondrial membrane potential (MMP) and found that TBEV infection induced the hyperpolarization of MMP. Moreover, a transient increase of gene expression of stress-induced antioxidative enzymes, like p62, Gclm and Hmox1, was detected. Next, we evaluated the ER stress upon TBEV infection by analysing unfolded protein responses (UPR). We found that infection induced gene expression of two general sensors BiP and CHOP and activated the IRE1 pathway of UPR. Finally, since the natural transmission route of TBEV from its tick vector to the host is mediated via tick saliva, the impact of tick saliva from Ixodes ricinus on stress pathways in TBEV-infected cells was tested. We observed only marginal potentiation of UPR pathway. In conclusion, we found that TBEV infection of PMJ2-R cells elicits the changes in redox balance and triggers cellular stress defences, including antioxidant responses and the IRE1 pathway of UPR. Importantly, our results revealed the negative effect of stress-evoked events on TBEV replication and only marginal impact of tick saliva on stress cellular pathways.

• MeSH
• buněčné linie MeSH
• klíšťová encefalitida * MeSH
• myši MeSH
• peroxid vodíku metabolismus   MeSH
• protein-serin-threoninkinasy metabolismus   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• replikace viru MeSH
• viry klíšťové encefalitidy * genetika   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND AND PURPOSE: Dysfunction of the airway defence system in Huntington's disease (HD) is a significant but often overlooked problem. Although expiratory muscle strength training (EMST) is frequently utilized in cough effectiveness treatment, its specific impact in HD patients has not yet been explored. This study investigated the effects of EMST on voluntary peak cough flow (vPCF) in HD patients and evaluated the retention of potential gains post-intervention. METHODS: In this prospective case-controlled trial, 29 HD patients completed an 8-week wait-to-start period, which served to identify the natural development of expiratory muscle strength and vPCF. This was followed by 8 weeks of EMST training and an additional 8 weeks of follow-up. The study's outcome parameters, vPCF and maximum expiratory pressure (MEP), were measured against those of age- and sex-matched healthy controls. RESULTS: Huntington's disease patients had significantly lower MEP (p < 0.001) and vPCF (p = 0.012) compared to healthy controls at baseline. Following the EMST, significant improvements in MEP (d = 1.39, p < 0.001) and vPCF (d = 0.77, p = 0.001) were observed, with HD patients reaching the cough performance levels of healthy subjects. However, these gains diminished during the follow-up, with a significant decline in vPCF (d = -0.451, p = 0.03) and in MEP (d = -0.71; p = 0.002). CONCLUSIONS: Expiratory muscle strength training improves expiratory muscle strength and voluntary cough effectiveness in HD patients, but an ongoing maintenance programme is necessary to sustain the improvements.

• MeSH
• dechová cvičení metody   MeSH
• dospělí MeSH
• dýchací svaly * patofyziologie   MeSH
• Huntingtonova nemoc * patofyziologie   komplikace   rehabilitace   MeSH
• kašel * patofyziologie   etiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• odporový trénink metody   MeSH
• prospektivní studie MeSH
• studie případů a kontrol MeSH
• svalová síla * fyziologie   MeSH
• výsledek terapie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

OBJECTIVES: The Slovak Medical University (SMU) holds a unique position in the health education system in Slovakia. It has a direct connection to the health sector, allowing health education to reflect the actual needs in this field. Because of increasing importance of public health in the last decades, more attention must be given to disease prevention and the promotion of healthy lifestyles. We aim to highlight the main characteristics of health higher education at one of the specialized health universities in Slovakia, with a particular focus on public health and its practical impacts. METHODS: We analysed the available legal regulations for postgraduate studies in Slovakia and the officially valid documents of the Faculty of Public Health (FPH) and the Slovak Medical University in accordance with the accredited study programme in Public Health. Archived data from the Department of Science, Research, and Doctoral Studies of the Faculty over the past 10 years were used for the analysis of postgraduate studies (2013-2023). RESULTS: PhD studies in Slovakia are conducted in accordance with Act No. 131/2002 Coll. on Higher Education Institutions and on amendments to certain acts. There are two forms of PhD study in Slovakia: full-time and external. The evaluation of study results is based on the credit system. The doctoral study programme proceeds according to an individual study plan under the guidance of the advisor. The PhD study concludes with the defence of the dissertation, which serves as the final thesis. A total of 97 students have graduated at FPH SMU in Public Health in the last 10 years. The majority of graduates were females (68% vs. 32% males) and studied in the external form of study (80.4% vs. 19.6% in the full-time programme). The most frequent research topics at FPH SMU in the last 10 years included Epidemiology and Prevention of Non-communicable (21.7%) and Infectious Diseases (11.3%), Health Management and Policy (17.5%), Environmental Health (15.5%), as well as Occupational Health (13.4%). CONCLUSION: High-quality and innovative postgraduate education in public health plays a crucial role in this field, preparing experts for the public health services. From a quality perspective, it is substantial to share experiences with various study programmes across the European region, as well as with other universities. Graduates of the Faculty of Public Health are highly sought-after professionals with diverse career opportunities not only in Slovakia but also within the European Union, other countries, and various important international institutions.

• MeSH
• akademický sbor lékařské fakulty MeSH
• lidé MeSH
• školy veřejného zdravotnictví organizace a řízení   MeSH
• studium vysokoškolské * MeSH
• univerzity MeSH
• veřejné zdravotnictví * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Slovenská republika MeSH

This paper deals with karate activity in the areas of physical culture in the broad sense. It is a concise and clear approach to the topic, which aimed to conceptualise the inclusion of karate in areas of physical culture as a contemporarily attractive physical activity. Karate was described here as a combat sport, martial art, and self-defence system in the following areas of physical culture: physical education, physiotherapy, recreation, sports, and tourism. Reference here was made to physical activity focused on bunkai (circuit interpretation exercises), kata (circuit exercises), kihon (technical exercises), and kumite (combat exercises). It has been determined that practitioners' involvement in karate culture affects their level of sense of coherence. In addition, the conditioning for using karate in physical culture to the maintenance of health was mentioned. Here, reference was made to the perceptual perspective of the bodily and mental practice of karate for health. It has been established that this can be effective by adopting only the objectives of karate as martial art. Based on the above, it was concluded that practising karate is present in all areas of physical culture as combat sport, martial art, and self-defence system, but practising karate only as martial art provides opportunities for maintenance of health.

• Publikační typ
• časopisecké články MeSH

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and subsequent COVID-19 has spread world-wide and become pandemic with about 7 million deaths reported so far. Interethnic variability of the disease has been described, but a significant part of the differences remain unexplained and may be attributable to genetic factors. AIM: To analyse genetic factors potentially influencing COVID-19 susceptibility and severity in European Roma minority. SUBJECTS AND METHODS: Two genetic determinants, within OAS-1 (2-prime,5-prime-oligoadenylate synthetase 1, a key protein in the defence against viral infection; it activates RNases that degrade viral RNAs; rs4767027 has been analysed) and LZTFL1 (leucine zipper transcription factor-like 1, expressed in the lung respiratory epithelium; rs35044562 has been analysed) genes were screened in a population-sample of Czech Roma (N = 302) and majority population (N = 2,559). RESULTS: For both polymorphisms, Roma subjects were more likely carriers of at least one risky allele for both rs4767027-C (p < 0.001) and rs35044562-G (p < 0.00001) polymorphism. There were only 5.3% Roma subjects without at least one risky allele in comparison with 10.1% in the majority population (p < 0.01). CONCLUSIONS: It is possible that different genetic background plays an important role in increased prevalence of COVID-19 in the Roma minority.

• MeSH
• 2',5'-oligoadenylátsynthetasa genetika   MeSH
• COVID-19 * genetika   epidemiologie   MeSH
• dospělí MeSH
• genetická predispozice k nemoci MeSH
• jednonukleotidový polymorfismus MeSH
• lidé středního věku MeSH
• lidé MeSH
• mutace MeSH
• neandertálci * genetika   MeSH
• prevalence MeSH
• Romové * genetika   MeSH
• SARS-CoV-2 * MeSH
• senioři MeSH
• transkripční faktory genetika   MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

Adhesion G protein-coupled receptors (aGPCRs) play an important role in neurodevelopment, immune defence and cancer; however, their role throughout viral infections is mostly unexplored. We have been searching for specific aGPCRs involved in SARS-CoV-2 infection of mammalian cells. In the present study, we infected human epithelial cell lines derived from lung adenocarcinoma (Calu-3) and colorectal carcinoma (Caco-2) with SARS-CoV-2 in order to analyse changes in the level of mRNA encoding individual aGPCRs at 6 and 12 h post infection. Based on significantly altered mRNA levels, we identified four aGPCR candidates-ADGRB3/BAI3, ADGRD1/GPR133, ADGRG7/GPR128 and ADGRV1/GPR98. Of these receptors, ADGRD1/GPR133 and ADGRG7/GPR128 showed the largest increase in mRNA levels in SARS-CoV-2-infected Calu-3 cells, whereas no increase was observed with heat-inactivated SARS-CoV-2 and virus-cleared conditioned media. Next, using specific siRNA, we downregulated the aGPCR candidates and analysed SARS-CoV-2 entry, replication and infectivity in both cell lines. We observed a significant decrease in the amount of SARS-CoV-2 newly released into the culture media by cells with downregulated ADGRD1/GPR133 and ADGRG7/GPR128. In addition, using a plaque assay, we observed a reduction in SARS-CoV-2 infectivity in Calu-3 cells. In summary, our data suggest that selected aGPCRs might play a role during SARS-CoV-2 infection of mammalian cells.

• MeSH
• adenokarcinom plic * genetika   virologie   patologie   metabolismus   MeSH
• Caco-2 buňky MeSH
• COVID-19 * genetika   virologie   metabolismus   MeSH
• lidé MeSH
• messenger RNA * genetika   metabolismus   MeSH
• nádorové buněčné linie MeSH
• nádory plic genetika   virologie   patologie   metabolismus   MeSH
• receptory spřažené s G-proteiny * metabolismus   genetika   MeSH
• SARS-CoV-2 * genetika   fyziologie   metabolismus   MeSH
• upregulace * genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Cíl práce: Rizikové chování je typické pro období adolescence a mladou dospělost, ale následky mohou přetrvávat po zbytek života – a to ve všech jeho oblastech – fyzické, psychické i sociální. Za spouštěče rizikového chování jsou považovány náročné situace, stres stejně jako pocity nedostatečně podnětného života jedince. Zcela zásadní roli proto představuje prevence. Cílem studie bylo analyzovat a popsat současný stav ve skupině vysokoškolských studentů v oblasti závislostního a rizikového sexuálního chování. Materiál a metodika: Mezi lety 2019–2022 byli studenti vysokých škol ve východních Čechách – Univerzity Hradec Králové (UHK), Univerzity Karlovy (UK), Univerzity obrany (UNOB) a Univerzity Pardubice (UPCE) osloveni a požádáni o vyplnění dotazníkového šetření zaměřeného na závislostní a rizikové sexuální chování. Na základě rešeršní strategie byly sestaveny výzkumné hypotézy, které byly následně zpracovány do tazatelských otázek. Výzkumné hypotézy byly testovány s využitím Pearsonova testu. Návratnost distribuovaného dotazníku dosáhla 84 %. Po vyřazení nevalidních odpovědí se výzkumného šetření zúčastnilo 157 respondentů – 64 % žen, 36 % mužů. Věkové rozložení studentů se pohybovalo v intervalu od 19 do 32 let. Výsledky: V oblasti problematiky návykového chování jsme posuzovali látkové a nelátkové závislosti. Z hlediska návykových látek se jednalo o otázku kouření cigaret, které uvedlo 14,6 % z celkového počtu tázaných vysokoškolských studentů. To je o 50 % méně než vídáme u běžné populace stejného věku (pod běžnou populací rozumíme skupinu oslovených osob do provedených studií zvolenou stratifikovaným náhodným výběrem na základě věku). Naopak, výrazně vyšších hodnot oproti běžné populaci ve stejném věku bylo zjištěno v případě užívání nelegálních návykových látek – marihuany, sedativ bez předpisu lékaře, kokainu či halucinogenů. V oblasti rizikového sexuálního chování se výzkum dotazoval na pohlavní styk s náhodnou známostí. Tuto zkušenost uvedl každý čtvrtý respondent. V 83,3 % bylo toto chování ovlivněno užitím návykové látky. Výzkum poukázal na fakt vysoké frekvence salutoprotektivních faktorů (např. zájem o sport uvedlo 87,9 % dotázaných). Preventivním faktorem v oblasti rizikového chování je pocit sociální opory v podobě přátel (uvedlo 74,5 % respondentů) či rodiny (uvedlo 72,6 % dotázaných). Závěr: Cílem výzkumného šetření bylo zjištění prevalence rizikového chování zaměřeného na chování závislostní a sexuálně rizi- kové mezi studenty vysokých škol a dále jeho prevence – otázka salutoprotektivních faktorů při zvýšeném stresu či uvědomělost sociální opory. U vysokoškolských studentů jsme předpokládali protektivně působící vyšší edukaci, která se ale v našem šetření neprojevila. Naopak rizikovým faktorem se ukázala zátěž vyvolaná vysokoškolským studiem a tlakem na studijní úspěšnost. Výsledky výzkumu přináší možné pohledy na nové způsoby uchopení prevence z hlediska vyšší efektivity a poukazuje na zásadní problémy ve sledovaných oblastech v české populaci vysokoškolských studentů.

Aim: Risk behaviour is typical of adolescence and young adulthood, but the consequences can persist for the rest of life – in all domains – physical, psychological, and social. Challenging situations, stress, as well as feelings of a lack of stimulation in an individual’s life are considered to be triggers for risky behaviours. Prevention therefore plays a crucial role. The aim of the study was to analyse the current situation in addictive and risky sexual behaviours in a group of university students. Material and Methods: Between 2019 and 2022, students of universities in East Bohemia – University of Hradec Králové (UHK), Charles University (UK), University of Defence (UNOB), and University of Pardubice (UPCE) – were approached and asked to complete a questionnaire survey focused on addictive and risky sexual behaviours. Based on the search strategy, research hypotheses were constructed, which were subsequently developed into interview questions. The research hypotheses were tested using Pearson’s test. The questionnaire return rate was 84%. After eliminating invalid responses, 157 participants were included in the survey – 64% female, 36% male, age range 19–32 years. Results: In the area of addictive behaviours, the focus was on substance and non-substance addictions. In terms of addictive substances, a question addressed cigarette smoking, which was reported by 14.6% of the survey participants. This is 50% less than what we see in the general population of the same age (by general population we mean the group of people approached for the surveys conducted, chosen by stratified random sampling based on age). In contrast, significantly higher rates of university students reported the use of illicit substances – marijuana, sedatives/tranquilizers without a doctor’s prescription, cocaine, or hallucinogens compared to the age-matched general population. In the area of risky sexual behaviours, a question was included about sexual intercourse with casual acquaintances. It was experienced by one in four respondents. In 83.3%, this behaviour was influenced by the use of an addictive substance. The survey pointed to the high rates of salutoprotective factors (e.g. interest in sports was reported by 87.9% of respondents). Perceived social support from friends (reported by 74.5% of respondents) or family (reported by 72.6% of respondents) is a preventive factor for risky behaviours. Conclusions: The aim of the survey was to determine the prevalence of high-risk addictive and sexual behaviours among university students and possible prevention measures. Salutoprotective factors during increased stress or awareness of social support seem to be of benefit. We assumed a protective effect of higher education, but this survey did not show it. On the contrary, the stress induced by university studies and the pressure on academic performance appeared to be a risk factor. The results of the survey provide insights into new approaches to the prevention to improve its effectiveness and point to major problems to be addressed in Czech university students.

• MeSH
• dospělí MeSH
• kouření epidemiologie   MeSH
• lidé MeSH
• mladý dospělý MeSH
• návykové chování epidemiologie   etiologie   prevence a kontrola   MeSH
• pití alkoholu na vysoké škole MeSH
• průzkumy a dotazníky MeSH
• psychotropní léky MeSH
• rizikový sex statistika a číselné údaje   MeSH
• studenti * statistika a číselné údaje   MeSH
• univerzity MeSH
• zdravotně rizikové chování * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH